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LIM1 (LHX1) (NM_005568) Human Tagged ORF Clone
CAT＃: RC210977
LHX1 (Myc-DDK-tagged)-Human LIM homeobox 1 (LHX1)
ORF Plasmid: tGFP
"NM_005568" in other vectors (6)
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CNY 3,656.00
CNY 3,990.00
| Cited in 1 publication. |
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | LIM-1; LIM1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC210977 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTTCACTGTGCCGGCTGCAAAAGGCCCATCCTGGACCGCTTTCTCTTGAACGTGCTGGACAGGGCCT GGCACGTCAAGTGCGTCCAGTGCTGTGAATGTAAATGCAACCTGACCGAGAAGTGCTTCTCCAGGGAAGG CAAACTCTACTGCAAGAACGACTTCTTCCGGTGTTTCGGTACCAAATGCGCAGGCTGCGCTCAGGGCATC TCCCCTAGCGACCTGGTGCGGAGAGCGCGGAGCAAAGTGTTTCACCTGAACTGCTTCACCTGCATGATGT GTAACAAGCAGCTCTCCACTGGCGAGGAACTCTACATCATCGACGAGAATAAGTTCGTCTGCAAAGAGGA TTACCTAAGTAACAGCAGTGTTGCCAAAGAGAACAGCCTTCACTCGGCCACCACGGGCAGTGACCCCAGT TTGTCTCCGGATTCCCAAGACCCGTCGCAGGACGACGCCAAGGACTCGGAGAGCGCCAACGTGTCGGACA AGGAAGCGGGTAGCAACGAGAATGACGACCAGAACCTGGGCGCCAAGCGGCGGGGACCGCGCACCACCAT CAAAGCCAAGCAGCTGGAGACGCTGAAGGCCGCCTTCGCTGCTACACCCAAGCCCACCCGCCACATCCGC GAGCAGCTGGCGCAGGAGACCGGCCTCAACATGCGCGTCATTCAGGTCTGGTTCCAGAACCGGCGCTCCA AGGAGCGGAGGATGAAGCAGCTGAGCGCCCTGGGCGCCCGGCGCCACGCCTTCTTCCGCAGTCCGCGCCG GATGCGGCCGCTGGTGGACCGCCTGGAGCCGGGCGAGCTCATCCCCAATGGTCCCTTCTCCTTCTACGGA GATTACCAGAGCGAGTACTACGGGCCCGGGGGCAACTACGACTTCTTCCCGCAAGGCCCCCCGTCCTCGC AGGCCCAGACACCAGTGGACCTACCCTTCGTGCCGTCATCTGGGCCGTCCGGGACGCCCCTGGGTGGCCT GGAGCACCCGCTGCCGGGCCACCACCCGTCGAGCGAGGCGCAGCGGTTTACCGACATCCTGGCGCACCCA CCCGGGGACTCGCCCAGCCCCGAGCCCAGCCTGCCCGGGCCTCTGCACTCCATGTCGGCCGAGGTCTTCG GACCCAGCCCGCCCTTCTCGTCGCTGTCGGTCAACGGTGGGGCGAGCTACGGAAACCACCTGTCCCACCC CCCCGAAATGAACGAGGCGGCCGTGTGG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC210977 protein sequence
Red=Cloning site Green=Tags(s) MVHCAGCKRPILDRFLLNVLDRAWHVKCVQCCECKCNLTEKCFSREGKLYCKNDFFRCFGTKCAGCAQGI SPSDLVRRARSKVFHLNCFTCMMCNKQLSTGEELYIIDENKFVCKEDYLSNSSVAKENSLHSATTGSDPS LSPDSQDPSQDDAKDSESANVSDKEAGSNENDDQNLGAKRRGPRTTIKAKQLETLKAAFAATPKPTRHIR EQLAQETGLNMRVIQVWFQNRRSKERRMKQLSALGARRHAFFRSPRRMRPLVDRLEPGELIPNGPFSFYG DYQSEYYGPGGNYDFFPQGPPSSQAQTPVDLPFVPSSGPSGTPLGGLEHPLPGHHPSSEAQRFTDILAHP PGDSPSPEPSLPGPLHSMSAEVFGPSPPFSSLSVNGGASYGNHLSHPPEMNEAAVW TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_005568 |
| ORF Size | 1218 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_005568.5 |
| RefSeq Size | 3428 bp |
| RefSeq ORF | 1221 bp |
| Locus ID | 3975 |
| UniProt ID | P48742 |
| Domains | homeobox, LIM |
| Protein Families | ES Cell Differentiation/IPS, Transcription Factors |
| MW | 44.8 kDa |
| Gene Summary | This gene encodes a member of a large protein family which contains the LIM domain, a unique cysteine-rich zinc-binding domain. The encoded protein is a transcription factor important for the development of the renal and urogenital systems. This gene is a candidate for Mayer-Rokitansky-Kuster-Hauser syndrome, a disorder characterized by anomalies in the female genital tract. [provided by RefSeq, Dec 2010] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
The nephrogenic potential of the transcription factors osr1, osr2, hnf1b, lhx1 and pax8 assessed in Xenopus animal caps
,null,
BMC Developmental Biology
,PubMed ID 21281489
[LIM1]
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Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC210977L1 | Lenti ORF clone of Human LIM homeobox 1 (LHX1), Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC210977L2 | Lenti ORF clone of Human LIM homeobox 1 (LHX1), mGFP tagged |
CNY 5,890.00 |
|
| RC210977L3 | Lenti ORF clone of Human LIM homeobox 1 (LHX1), Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC210977L4 | Lenti ORF clone of Human LIM homeobox 1 (LHX1), mGFP tagged |
CNY 6,056.00 |
|
| RG210977 | LHX1 (tGFP-tagged) - Human LIM homeobox 1 (LHX1) |
CNY 5,256.00 |
|
| SC310433 | LHX1 (untagged)-Human LIM homeobox 1 (LHX1) |
CNY 3,656.00 |
