Securin (PTTG1) (NM_004219) Human Tagged ORF Clone
CAT#: RC211038
PTTG1 (Myc-DDK-tagged)-Human pituitary tumor-transforming 1 (PTTG1)
ORF Plasmid: tGFP
"NM_004219" in other vectors (6)
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CNY 3,600.00
CNY 3,705.00
Cited in 5 publications. |
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | EAP1; HPTTG; PTTG; TUTR1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC211038 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTACTCTGATCTATGTTGATAAGGAAAATGGAGAACCAGGCACCCGTGTGGTTGCTAAGGATGGGC TGAAGCTGGGGTCTGGACCTTCAATCAAAGCCTTAGATGGGAGATCTCAAGTTTCAACACCACGTTTTGG CAAAACGTTCGATGCCCCACCAGCCTTACCTAAAGCTACTAGAAAGGCTTTGGGAACTGTCAACAGAGCT ACAGAAAAGTCTGTAAAGACCAAGGGACCCCTCAAACAAAAACAGCCAAGCTTTTCTGCCAAAAAGATGA CTGAGAAGACTGTTAAAGCAAAAAGCTCTGTTCCTGCCTCAGATGATGCCTATCCAGAAATAGAAAAATT CTTTCCCTTCAATCCTCTAGACTTTGAGAGTTTTGACCTGCCTGAAGAGCACCAGATTGCGCACCTCCCC TTGAGTGGAGTGCCTCTCATGATCCTTGACGAGGAGAGAGAGCTTGAAAAGCTGTTTCAGCTGGGCCCCC CTTCACCTGTGAAGATGCCCTCTCCACCATGGGAATCCAATCTGTTGCAGTCTCCTTCAAGCATTCTGTC GACCCTGGATGTTGAATTGCCACCTGTTTGCTGTGACATAGATATT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC211038 protein sequence
Red=Cloning site Green=Tags(s) MATLIYVDKENGEPGTRVVAKDGLKLGSGPSIKALDGRSQVSTPRFGKTFDAPPALPKATRKALGTVNRA TEKSVKTKGPLKQKQPSFSAKKMTEKTVKAKSSVPASDDAYPEIEKFFPFNPLDFESFDLPEEHQIAHLP LSGVPLMILDEERELEKLFQLGPPSPVKMPSPPWESNLLQSPSSILSTLDVELPPVCCDIDI TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_004219 |
ORF Size | 606 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_004219.4 |
RefSeq Size | 786 bp |
RefSeq ORF | 609 bp |
Locus ID | 9232 |
UniProt ID | O95997 |
Domains | Securin |
Protein Families | Druggable Genome, Transcription Factors |
Protein Pathways | Cell cycle, Oocyte meiosis |
MW | 22 kDa |
Gene Summary | The encoded protein is a homolog of yeast securin proteins, which prevent separins from promoting sister chromatid separation. It is an anaphase-promoting complex (APC) substrate that associates with a separin until activation of the APC. The gene product has transforming activity in vitro and tumorigenic activity in vivo, and the gene is highly expressed in various tumors. The gene product contains 2 PXXP motifs, which are required for its transforming and tumorigenic activities, as well as for its stimulation of basic fibroblast growth factor expression. It also contains a destruction box (D box) that is required for its degradation by the APC. The acidic C-terminal region of the encoded protein can act as a transactivation domain. The gene product is mainly a cytosolic protein, although it partially localizes in the nucleus. Three transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Sep 2013] |
Citations (5)
The use of this cDNA Clones has been cited in the following citations: |
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Upregulation of PTTG1 is associated with poor prognosis in prostate cancer
,Fraune, C;Yehorov, S;Luebke, AM;Steurer, S;Hube-Magg, C;Büscheck, F;Höflmayer, D;Tsourlakis, MC;Clauditz, TS;Simon, R;Sauter, G;Weidemann, S;Dum, D;Kind, S;Minner, S;Schlomm, T;Huland, H;Heinzer, H;Graefen, M;Burandt, E;,
Pathol. Int.
,PubMed ID 32314536
[Securin]
|
aarF domain containing kinase 5 gene promotes invasion and migration of lung cancer cells through ADCK5-SOX9-PTTG1 pathway
,Qiu, M;Li, G;Wang, P;Li, X;Lai, F;Luo, R;Liu, B;Lin, J;,
Exp. Cell Res.
,PubMed ID 32277958
[Securin]
|
Targeting the PTTG1 oncogene impairs proliferation and invasiveness of melanoma cells sensitive or with acquired resistance to the BRAF inhibitor dabrafenib
,Caporali, S;Alvino, E;Lacal, PM;Ruffini, F;Levati, L;Bonmassar, L;Scoppola, A;Marchetti, P;Mastroeni, S;Antonini Cappellini, GC;D'Atri, S;,
Oncotarget
,PubMed ID 29371923
[Securin]
|
Berberine Inhibits Uterine Leiomyoma Cell Proliferation via Downregulation of Cyclooxygenase 2 and Pituitary Tumor-Transforming Gene 1
,Chuang, TY;Min, J;Wu, HL;McCrary, C;Layman, LC;Diamond, MP;Azziz, R;Al-Hendy, A;Chen, YH;,
Reprod Sci
,PubMed ID 27799459
[Securin]
|
Pituitary tumor-transforming gene 1 regulates invasion of prostate cancer cells through MMP13
,Lin, YH;Tian, Y;Wang, JS;Jiang, YG;Luo, Y;Chen, YT;,
Tumour Biol.
,PubMed ID 26201898
[Securin]
|
Documents
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FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC211038L1 | Lenti ORF clone of Human pituitary tumor-transforming 1 (PTTG1), Myc-DDK-tagged |
CNY 6,000.00 |
|
RC211038L2 | Lenti ORF clone of Human pituitary tumor-transforming 1 (PTTG1), mGFP tagged |
CNY 5,890.00 |
|
RC211038L3 | Lenti ORF clone of Human pituitary tumor-transforming 1 (PTTG1), Myc-DDK-tagged |
CNY 6,000.00 |
|
RC211038L4 | Lenti ORF clone of Human pituitary tumor-transforming 1 (PTTG1), mGFP tagged |
CNY 6,000.00 |
|
RG211038 | PTTG1 (tGFP-tagged) - Human pituitary tumor-transforming 1 (PTTG1) |
CNY 5,200.00 |
|
SC117528 | PTTG1 (untagged)-Human pituitary tumor-transforming 1 (PTTG1) |
CNY 3,600.00 |
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