United States
Germany
Japan
United Kingdom
China
CDC2L2 (NM_033532) Human Tagged ORF Clone
CAT＃: RC211124
CDC2L2 (Myc-DDK-tagged)-Human cell division cycle 2-like 2 (PITSLRE proteins) (CDC2L2), transcript variant 6
ORF Plasmid: tGFP
"NM_033532" in other vectors (5)
Need custom modification / cloning service?
Get a free quote
CNY 3,656.00
CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | CDC2L3, p58GTA, PITSLRE, CDK11-p46, CDK11-p58, MGC131975, CDK11-p110 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC211124 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGAACGAGAAAATGAAAACCACCTCTTGGTTGTTCCAGAGTCACGGTTCGACCGAGATTCCGGGGA GAGTGAAGAAGCAGAGGAAGAAGTGGGGCTGCCGGAGCGTCGAGGAGTTCCAGTGCCTGAACAGGATCGA GGAGGGCACCTATGGAGTGGTCTACAGAGCAAAAGACAAGAAAACAGATGAAATTGTGGCTCTAAAGCGG CTGAAGATGGAGAAGGAGAAGGAGGGCTTCCCGATCACGTCCCTGAGGGAGATCAACACCATCCTCAAGG CCCAGCATCCCAACATTGTCACCGTTAGAGAGATTGTGGTGGGCAGCAACATGGACAAGATCTACATCGT GATGAACTATGTGGAGCACGACCTCAAGAGCCTGATGGAGACCATGAAACAGCCCTTCCTGCCAGGGGAG GTGAAGACCCTGATGATCCAGCTGCTGCGTGGGGTGAAACACCTGCACGACAACTGGATCCTGCACCGTG ACCTCAAGACGTCCAACCTGCTGCTGAGCCACGCCGGCATCCTCAAGGTGGGTGATTTTGGGCTGGCGCG GGAGTACGGATCCCCTCTGAAGGCCTACACCCCGGTCGTGGTGACCCAGTGGTACCGCGCCCCAGAGCTG CTGCTTGGTGCCAAGGAATACTCCACGGCCGTGGACATGTGGTCAGTGGGCTGCATCTTCGGGGAGCTGC TGACTCAGAAGCCTCTGTTCCCCGGGAATTCGGAAATCGATCAGATCAACAAAGTGTTCAAGGAGCTGGG GACCCCCAGTGAGAAAATCTGGCCCGGCTACAGTGAGCTCCCAGTAGTCAAGAAGATGACCTTCAGCGAG CACCCCTACAACAACCTCCGCAAGCGCTTCGGGGCTCTGCTCTCAGACCAGGGCTTCGACCTCATGAACA AGTTCCTGACCTACTTCCCCGGGAGGAGGATCAGCGCTGAGGACGGCCTCAAGCATGAGTATTTCCGCGA GACCCCCCTCCCCATCGACCCCTCCATGTTCCCCACGTGGCCCGCCAAGAGCGAGCAGCAGCGTGTGAAG CGGGGCACCAGCCCGAGGCCCCCTGAGGGAGGCCTGGGCTACAGCCAGCTGGGTGACGACGACCTGAAGG AGACGGGCTTCCACCTTACCACCACGAACCAGGGGGCCTCTGCCGCGGGCCCCGGCTTCAGCCTCAAGTT C ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC211124 protein sequence
Red=Cloning site Green=Tags(s) MKNEKMKTTSWLFQSHGSTEIPGRVKKQRKKWGCRSVEEFQCLNRIEEGTYGVVYRAKDKKTDEIVALKR LKMEKEKEGFPITSLREINTILKAQHPNIVTVREIVVGSNMDKIYIVMNYVEHDLKSLMETMKQPFLPGE VKTLMIQLLRGVKHLHDNWILHRDLKTSNLLLSHAGILKVGDFGLAREYGSPLKAYTPVVVTQWYRAPEL LLGAKEYSTAVDMWSVGCIFGELLTQKPLFPGNSEIDQINKVFKELGTPSEKIWPGYSELPVVKKMTFSE HPYNNLRKRFGALLSDQGFDLMNKFLTYFPGRRISAEDGLKHEYFRETPLPIDPSMFPTWPAKSEQQRVK RGTSPRPPEGGLGYSQLGDDDLKETGFHLTTTNQGASAAGPGFSLKF TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_033532 |
| ORF Size | 1191 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_033532.1, NP_277074.1 |
| RefSeq Size | 2362 bp |
| RefSeq ORF | 1193 bp |
| Locus ID | 985 |
| Protein Families | Protein Kinase, Transcription Factors |
| MW | 45.2 kDa |
| Gene Summary | This gene encodes a member of the p34Cdc2 protein kinase family. p34Cdc2 kinase family members are known to be essential for eukaryotic cell cycle control. This gene is in close proximity to CDC2L1, a nearly identical gene in the same chromosomal region. The gene loci including this gene, CDC2L1, as well as metalloprotease MMP21/22, consist of two identical, tandemly linked genomic regions, which are thought to be a part of the larger region that has been duplicated. This gene and CDC2L1 were shown to be deleted or altered frequently in neuroblastoma with amplified MYCN genes. The protein kinase encoded by this gene could be cleaved by caspases and was demonstrated to play roles in cell apoptosis. Many transcript variants encoding several different isoforms have been found for this gene, but the full-length nature of only two have been determined so far. |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC211124L1 | Lenti ORF clone of Human cell division cycle 2-like 2 (PITSLRE proteins) (CDC2L2), transcript variant 6, Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC211124L2 | Lenti ORF clone of Human cell division cycle 2-like 2 (PITSLRE proteins) (CDC2L2), transcript variant 6, mGFP tagged |
CNY 5,890.00 |
|
| RC211124L3 | Lenti ORF clone of Human cell division cycle 2-like 2 (PITSLRE proteins) (CDC2L2), transcript variant 6, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC211124L4 | Lenti ORF clone of Human cell division cycle 2-like 2 (PITSLRE proteins) (CDC2L2), transcript variant 6, mGFP tagged |
CNY 5,890.00 |
|
| RG211124 | CDC2L2 (tGFP-tagged) - Human cell division cycle 2-like 2 (PITSLRE proteins) (CDC2L2), transcript variant 6 |
CNY 5,256.00 |
