PGK1 (NM_000291) Human Tagged Lenti ORF Clone
CAT#: RC211172L3
- LentiORF®
Lenti ORF clone of Human phosphoglycerate kinase 1 (PGK1), Myc-DDK-tagged
"NM_000291" in other vectors (6)
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CNY 5,890.00
Product images
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CNY 600.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | HEL-S-68p; MIG10; PGKA |
Vector | pLenti-C-Myc-DDK-P2A-Puro |
E. coli Selection | Chloramphenicol (34 ug/mL) |
Mammalian Cell Selection | Puromycin |
Sequence Data |
The ORF insert of this clone is exactly the same as(RC211172).
|
Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
ACCN | NM_000291 |
ORF Size | 1251 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_000291.2 |
RefSeq Size | 2439 bp |
RefSeq ORF | 1254 bp |
Locus ID | 5230 |
UniProt ID | P00558 |
Domains | PGK |
Protein Families | Druggable Genome |
Protein Pathways | Glycolysis / Gluconeogenesis, Metabolic pathways |
MW | 44.6 kDa |
Gene Summary | The protein encoded by this gene is a glycolytic enzyme that catalyzes the conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate. The encoded protein may also act as a cofactor for polymerase alpha. Additionally, this protein is secreted by tumor cells where it participates in angiogenesis by functioning to reduce disulfide bonds in the serine protease, plasmin, which consequently leads to the release of the tumor blood vessel inhibitor angiostatin. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Deficiency of the enzyme is associated with a wide range of clinical phenotypes hemolytic anemia and neurological impairment. Pseudogenes of this gene have been defined on chromosomes 19, 21 and the X chromosome. [provided by RefSeq, Jan 2014] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC211172 | PGK1 (Myc-DDK-tagged)-Human phosphoglycerate kinase 1 (PGK1) |
CNY 3,656.00 |
|
RC211172L1 | Lenti ORF clone of Human phosphoglycerate kinase 1 (PGK1), Myc-DDK-tagged |
CNY 6,056.00 |
|
RC211172L2 | Lenti ORF clone of Human phosphoglycerate kinase 1 (PGK1), mGFP tagged |
CNY 5,890.00 |
|
RC211172L4 | Lenti ORF clone of Human phosphoglycerate kinase 1 (PGK1), mGFP tagged |
CNY 5,890.00 |
|
RG211172 | PGK1 (tGFP-tagged) - Human phosphoglycerate kinase 1 (PGK1) |
CNY 5,256.00 |
|
SC119988 | PGK1 (untagged)-Human phosphoglycerate kinase 1 (PGK1) |
CNY 3,656.00 |