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DOCK2 (NM_004946) Human Tagged ORF Clone
CAT＃: RC211198
- TrueORF®
DOCK2 (Myc-DDK-tagged)-Human dedicator of cytokinesis 2 (DOCK2)
ORF Plasmid: tGFP
"NM_004946" in other vectors (3)
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CNY 13,780.00
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | IMD40 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC211198 representing NM_004946
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCCCCTGGCGCAAAGCTGACAAGGAGCGGCACGGCGTGGCCATATACAACTTCCAAGGCAGCGGAG CCCCCCAGCTCTCCCTGCAGATCGGCGATGTGGTGCGAATACAGGAGACGTGTGGAGACTGGTATAGGGG ATACCTCATAAAGCACAAAATGTTACAGGGCATTTTTCCTAAGTCATTTATCCACATCAAGGAAGTGACA GTTGAGAAAAGAAGAAATACTGAGAACATCATTCCTGCAGAAATTCCTCTGGCACAAGAAGTGACAACGA CACTTTGGGAATGGGGAAGCATCTGGAAACAACTCTATGTGGCCAGCAAAAAGGAGCGTTTTCTCCAGGT GCAGTCCATGATGTACGATCTGATGGAGTGGAGGTCCCAGCTTCTCTCAGGAACCTTACCCAAGGATGAG CTGAAGGAACTGAAGCAGAAAGTCACGTCCAAAATTGACTATGGCAACAAAATCCTTGAGCTTGATTTGA TTGTCAGAGATGAAGACGGAAATATCTTGGACCCTGATAATACCAGTGTCATCAGCTTGTTCCATGCACA TGAGGAAGCAACTGATAAAATCACAGAGCGTATCAAAGAAGAAATGTCAAAAGACCAGCCAGATTATGCA ATGTATTCCCGGATCTCCTCATCCCCCACCCATAGCCTCTATGTGTTTGTGAGAAACTTTGTGTGCAGAA TTGGGGAAGATGCTGAGCTCTTCATGTCTCTCTACGACCCCAACAAGCAAACGGTCATAAGTGAGAACTA CCTAGTGCGATGGGGCAGCCGGGGCTTCCCTAAGGAGATTGAGATGCTCAACAATCTGAAGGTGGTCTTC ACGGATCTTGGAAACAAAGACCTCAACAGGGATAAAATTTACTTGATTTGTCAAATAGTCCGGGTCGGCA AGATGGATCTTAAGGATACTGGTGCAAAGAAGTGCACGCAGGGACTGAGGAGGCCCTTTGGGGTGGCAGT TATGGATATAACAGACATCATCAAGGGGAAAGCAGAGAGTGATGAAGAAAAGCAGCACTTCATTCCTTTT CACCCGGTTACAGCTGAGAATGACTTCCTACACAGCCTGCTGGGCAAAGTCATAGCCTCCAAGGGGGACA GTGGAGGGCAAGGCCTCTGGGTGACCATGAAGATGCTGGTGGGTGACATCATTCAGATTCGCAAGGACTA TCCACACCTGGTGGACAGGACCACCGTGGTGGCCAGGAAGCTGGGATTCCCAGAGATCATCATGCCAGGG GATGTCAGGAACGACATCTACATTACTCTCTTACAAGGTGACTTTGACAAGTACAACAAGACCACACAGA GGAATGTGGAAGTCATCATGTGTGTGTGCGCGGAGGATGGCAAAACGCTGCCTAATGCAATTTGCGTGGG AGCAGGGGACAAGCCCATGAATGAGTATCGCTCCGTTGTGTACTATCAAGTCAAACAGCCACGCTGGATG GAAACAGTCAAGGTGGCTGTCCCTATTGAAGACATGCAGAGGATCCATCTGCGATTCATGTTTCGACATC GGTCATCTCTGGAATCTAAAGATAAAGGAGAAAAGAACTTTGCCATGTCCTATGTGAAGCTGATGAAAGA AGATGGGACTACTCTACACGATGGATTCCATGACTTAGTTGTCCTCAAGGGGGACAGCAAGAAGATGGAG GATGCCAGCGCATACCTGACCCTTCCTTCTTATCGACACCATGTGGAAAACAAGGGGGCCACGCTGAGCA GGAGCTCCAGCAGTGTTGGGGGGCTTTCTGTCAGCTCCCGGGATGTGTTCTCCATTTCCACCCTGGTGTG CTCCACAAAGCTCACTCAGAATGTGGGCTTGCTGGGTTTGCTGAAGTGGCGTATGAAGCCTCAACTGCTA CAGGAGAATTTAGAAAAGTTGAAGATTGTGGATGGAGAGGAAGTGGTGAAGTTTCTCCAGGATACTCTGG ATGCCCTCTTCAACATCATGATGGAGCATTCTCAAAGTGATGAATATGACATCCTCGTCTTTGATGCCTT GATTTACATAATAGGACTCATTGCAGACCGGAAATTTCAGCATTTCAACACCGTTCTGGAGGCTTACATC CAACAGCATTTCAGTGCGACCTTGGCTTACAAGAAATTGATGACAGTGCTGAAGACTTACTTGGATACCT CCAGCAGAGGGGAGCAATGTGAGCCAATCCTAAGAACGCTGAAGGCTTTGGAATATGTGTTCAAGTTCAT TGTTCGGTCGAGGACATTATTTTCACAGCTTTATGAAGGCAAAGAACAGATGGAGTTTGAAGAATCCATG AGACGGCTCTTTGAATCCATCAACAATCTGATGAAAAGTCAATACAAAACTACCATCCTTTTGCAGGTGG CGGCTTTGAAATACATCCCATCTGTCCTGCATGATGTAGAAATGGTCTTTGATGCGAAGTTACTCAGCCA ACTCCTGTATGAGTTCTACACCTGCATCCCTCCTGTGAAACTCCAGAAGCAGAAAGTACAGTCTATGAAT GAGATAGTCCAGAGCAACCTCTTTAAAAAGCAAGAATGCCGGGACATTCTGCTTCCTGTCATCACCAAAG AGCTGAAGGAGCTGCTGGAGCAGAAGGATGACATGCAACACCAGGTCCTGGAGAGGAAGTACTGCGTTGA ATTGCTCAACAGCATCTTGGAAGTCCTTAGCTACCAGGATGCGGCCTTCACCTACCACCATATCCAGGAG ATCATGGTCCAGCTGCTGCGGACAGTGAACCGGACAGTCATCACCATGGGCCGGGATCACATTCTGATTA GTCACTTTGTGGCATGTATGACAGCCATCTTAAACCAGATGGGTGACCAGCACTACTCCTTCTACATTGA GACCTTCCAGACCAGCTCTGAACTTGTGGACTTCTTGATGGAGACCTTCATCATGTTCAAGGACCTCATT GGAAAGAACGTGTACCCTGGAGACTGGATGGCCATGAGCATGGTTCAAAACAGGGTCTTCCTGAGAGCTA TCAACAAGTTTGCAGAAACCATGAACCAGAAGTTCCTAGAACACACGAACTTTGAGTTCCAGCTGTGGAA CAACTATTTTCATCTGGCAGTGGCTTTTATCACCCAGGATTCTCTGCAGCTGGAGCAGTTCTCACACGCC AAATACAACAAAATCCTGAATAAGTATGGGGACATGAGACGGCTCATTGGCTTCTCCATCCGTGATATGT GGTACAAGCTTGGTCAGAACAAAATCTGCTTCATCCCAGGCATGGTAGGACCTATATTAGAGATGACACT TATCCCTGAGGCTGAGCTCCGGAAAGCCACCATACCAATCTTCTTCGACATGATGCTGTGTGAATATCAA AGAAGTGGGGATTTCAAAAAGTTTGAAAACGAAATCATCCTGAAGCTGGACCACGAGGTAGAAGGGGGCC GAGGCGACGAGCAGTACATGCAGCTCCTGGAGTCAATCCTGATGGAATGTGCTGCAGAGCACCCAACCAT TGCCAAGTCGGTGGAGAACTTCGTGAACCTGGTCAAAGGCCTCCTGGAGAAGCTGCTGGATTACCGGGGT GTGATGACAGATGAGAGCAAAGACAACCGCATGAGCTGCACCGTGAACCTGCTGAATTTCTACAAAGATA ACAACAGGGAGGAGATGTACATAAGGTACCTGTACAAACTCCGCGATCTTCACCTGGACTGTGACAATTA CACAGAGGCTGCCTACACGCTCCTTCTCCACACCTGGCTTCTCAAGTGGTCGGATGAGCAGTGTGCATCA CAGGTCATGCAGACAGGCCAGCAGCACCCCCAGACACACCGGCAGCTGAAGGAGACGCTCTACGAGACCA TCATAGGCTACTTTGACAAAGGAAAGATGTGGGAAGAGGCCATAAGTCTGTGCAAGGAGCTGGCGGAACA GTACGAGATGGAGATCTTTGACTATGAGCTGCTCAGCCAGAACCTGATCCAGCAGGCAAAATTCTATGAA AGCATCATGAAAATCCTCAGGCCCAAACCAGACTACTTTGCTGTTGGATACTACGGCCAGGGATTCCCCT CCTTCCTGCGGAACAAAGTGTTCATCTACCGCGGGAAGGAATATGAGCGAAGAGAAGATTTCCAGATGCA GCTGATGACCCAGTTCCCCAATGCAGAGAAGATGAACACCACCTCTGCCCCGGGAGATGATGTGAAGAAT GCCCCAGGCCAGTATATCCAGTGCTTCACTGTCCAGCCTGTCTTGGATGAACATCCCAGGTTCAAGAATA AGCCAGTGCCTGACCAGATTATAAACTTCTACAAATCCAACTACGTGCAAAGGTTCCACTACTCCCGGCC CGTGCGCAGGGGGACCGTAGACCCAGAGAATGAGTTTGCTTCCATGTGGATTGAGAGAACCTCCTTCGTG ACTGCATACAAGCTGCCGGGGATCCTGCGCTGGTTTGAGGTGGTGCACATGTCGCAGACCACAATTAGTC CTCTGGAGAATGCCATAGAAACCATGTCCACGGCCAATGAGAAGATCCTGATGATGATAAACCAGTACCA GAGTGATGAGACCCTCCCCATCAACCCACTCTCCATGCTCCTGAACGGGATTGTGGACCCTGCTGTCATG GGAGGCTTCGCCAAGTATGAGAAGGCCTTCTTCACTGAAGAGTATGTCAGGGACCACCCTGAGGACCAGG ACAAGCTGACCCACCTCAAGGACCTGATTGCATGGCAGATCCCCTTCTTGGGAGCTGGGATTAAGATCCA TGAGAAAAGGGTGTCAGATAACTTGCGACCCTTCCATGACCGGATGGAGGAATGTTTCAAGAACCTGAAA ATGAAGGTGGAGAAGGAGTACGGTGTCCGAGAGATGCCTGACTTTGACGACAGGAGAGTGGGCCGTCCCA GGTCTATGCTGCGCTCATACAGACAGATGTCCATCATCTCTCTGGCTTCCATGAATTCTGACTGCAGCAC CCCCAGCAAGCCTACCTCAGAGAGCTTTGACCTGGAATTAGCATCACCCAAGACGCCGAGAGTGGAGCAG GAGGAACCGATCTCCCCGGGGAGCACCCTGCCTGAGGTCAAGCTGCGGAGGTCCAAGAAGAGGACAAAGA GAAGCAGCGTAGTTTTTGCGGATGAGAAAGCAGCTGCAGAGTCGGACCTGAAGCGGCTTTCCAGGAAGCA TGAGTTCATGAGTGACACCAACCTCTCGGAGCATGCGGCCATCCCCCTCAAGGCGTCTGTCCTCTCTCAA ATGAGCTTTGCCAGCCAGTCCATGCCTACCATCCCAGCCCTGGCGCTCTCAGTGGCAGGCATCCCTGGGT TGGATGAGGCCAACACATCTCCCCGCCTCAGCCAGACCTTCCTCCAACTCTCAGATGGTGACAAGAAGAC ACTCACACGGAAGAAGGTCAATCAGTTCTTCAAGACAATGCTGGCCAGCAAATCGGCTGAAGAAGGCAAA CAGATCCCAGACTCGCTGTCCACGGACCTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC211198 representing NM_004946
Red=Cloning site Green=Tags(s) MAPWRKADKERHGVAIYNFQGSGAPQLSLQIGDVVRIQETCGDWYRGYLIKHKMLQGIFPKSFIHIKEVT VEKRRNTENIIPAEIPLAQEVTTTLWEWGSIWKQLYVASKKERFLQVQSMMYDLMEWRSQLLSGTLPKDE LKELKQKVTSKIDYGNKILELDLIVRDEDGNILDPDNTSVISLFHAHEEATDKITERIKEEMSKDQPDYA MYSRISSSPTHSLYVFVRNFVCRIGEDAELFMSLYDPNKQTVISENYLVRWGSRGFPKEIEMLNNLKVVF TDLGNKDLNRDKIYLICQIVRVGKMDLKDTGAKKCTQGLRRPFGVAVMDITDIIKGKAESDEEKQHFIPF HPVTAENDFLHSLLGKVIASKGDSGGQGLWVTMKMLVGDIIQIRKDYPHLVDRTTVVARKLGFPEIIMPG DVRNDIYITLLQGDFDKYNKTTQRNVEVIMCVCAEDGKTLPNAICVGAGDKPMNEYRSVVYYQVKQPRWM ETVKVAVPIEDMQRIHLRFMFRHRSSLESKDKGEKNFAMSYVKLMKEDGTTLHDGFHDLVVLKGDSKKME DASAYLTLPSYRHHVENKGATLSRSSSSVGGLSVSSRDVFSISTLVCSTKLTQNVGLLGLLKWRMKPQLL QENLEKLKIVDGEEVVKFLQDTLDALFNIMMEHSQSDEYDILVFDALIYIIGLIADRKFQHFNTVLEAYI QQHFSATLAYKKLMTVLKTYLDTSSRGEQCEPILRTLKALEYVFKFIVRSRTLFSQLYEGKEQMEFEESM RRLFESINNLMKSQYKTTILLQVAALKYIPSVLHDVEMVFDAKLLSQLLYEFYTCIPPVKLQKQKVQSMN EIVQSNLFKKQECRDILLPVITKELKELLEQKDDMQHQVLERKYCVELLNSILEVLSYQDAAFTYHHIQE IMVQLLRTVNRTVITMGRDHILISHFVACMTAILNQMGDQHYSFYIETFQTSSELVDFLMETFIMFKDLI GKNVYPGDWMAMSMVQNRVFLRAINKFAETMNQKFLEHTNFEFQLWNNYFHLAVAFITQDSLQLEQFSHA KYNKILNKYGDMRRLIGFSIRDMWYKLGQNKICFIPGMVGPILEMTLIPEAELRKATIPIFFDMMLCEYQ RSGDFKKFENEIILKLDHEVEGGRGDEQYMQLLESILMECAAEHPTIAKSVENFVNLVKGLLEKLLDYRG VMTDESKDNRMSCTVNLLNFYKDNNREEMYIRYLYKLRDLHLDCDNYTEAAYTLLLHTWLLKWSDEQCAS QVMQTGQQHPQTHRQLKETLYETIIGYFDKGKMWEEAISLCKELAEQYEMEIFDYELLSQNLIQQAKFYE SIMKILRPKPDYFAVGYYGQGFPSFLRNKVFIYRGKEYERREDFQMQLMTQFPNAEKMNTTSAPGDDVKN APGQYIQCFTVQPVLDEHPRFKNKPVPDQIINFYKSNYVQRFHYSRPVRRGTVDPENEFASMWIERTSFV TAYKLPGILRWFEVVHMSQTTISPLENAIETMSTANEKILMMINQYQSDETLPINPLSMLLNGIVDPAVM GGFAKYEKAFFTEEYVRDHPEDQDKLTHLKDLIAWQIPFLGAGIKIHEKRVSDNLRPFHDRMEECFKNLK MKVEKEYGVREMPDFDDRRVGRPRSMLRSYRQMSIISLASMNSDCSTPSKPTSESFDLELASPKTPRVEQ EEPISPGSTLPEVKLRRSKKRTKRSSVVFADEKAAAESDLKRLSRKHEFMSDTNLSEHAAIPLKASVLSQ MSFASQSMPTIPALALSVAGIPGLDEANTSPRLSQTFLQLSDGDKKTLTRKKVNQFFKTMLASKSAEEGK QIPDSLSTDL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_004946 |
| ORF Size | 5490 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_004946.3 |
| RefSeq Size | 6050 bp |
| RefSeq ORF | 5493 bp |
| Locus ID | 1794 |
| UniProt ID | Q92608 |
| Protein Families | Druggable Genome |
| Protein Pathways | Chemokine signaling pathway, Fc gamma R-mediated phagocytosis |
| MW | 211.8 kDa |
| Gene Summary | The protein encoded by this gene belongs to the CDM protein family. It is specifically expressed in hematopoietic cells and is predominantly expressed in peripheral blood leukocytes. The protein is involved in remodeling of the actin cytoskeleton required for lymphocyte migration in response to chemokine signaling. It activates members of the Rho family of GTPases, for example RAC1 and RAC2, by acting as a guanine nucleotide exchange factor (GEF) to exchange bound GDP for free GTP. Mutations in this gene result in immunodeficiency 40 (IMD40), a combined form of immunodeficiency that affects T cell number and function, also with variable defects in B cell and NK cell function. [provided by RefSeq, May 2018] |
Documents
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| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC211198L1 | Lenti ORF clone of Human dedicator of cytokinesis 2 (DOCK2), Myc-DDK-tagged |
CNY 15,680.00 |
|
| RG211198 | DOCK2 (tGFP-tagged) - Human dedicator of cytokinesis 2 (DOCK2) |
CNY 13,904.00 |
|
| SC107268 | DOCK2 (untagged)-Human dedicator of cytokinesis 2 (DOCK2) |
CNY 12,312.00 |
