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AGO3 (NM_177422) Human Tagged ORF Clone
CAT＃: RC211382
AGO3 (Myc-DDK-tagged)-Human eukaryotic translation initiation factor 2C, 3 (EIF2C3), transcript variant 2
ORF Plasmid: tGFP
"NM_177422" in other vectors (4)
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CNY 4,664.00
CNY 7,315.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | EIF2C3 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC211382 representing NM_177422
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTGTGAAGTTCTTGATATTCATAATATTGATGAGCAACCAAGACCTCTGACTGATTCTCATCGGGTAA AATTCACCAAAGAGATAAAAGGTTTGAAGGTTGAAGTGACTCATTGTGGAACAATGAGACGGAAATACCG TGTTTGTAATGTAACAAGGAGGCCTGCCAGTCATCAAACCTTTCCTTTACAGTTAGAAAACGGCCAAACT GTGGAGAGAACAGTAGCGCAGTATTTCAGAGAAAAGTATACTCTTCAGCTGAAGTACCCGCACCTTCCCT GTCTGCAAGTCGGGCAGGAACAGAAACACACCTACCTGCCACTAGAAGTCTGTAATATTGTGGCAGGGCA ACGATGTATCAAGAAGCTAACAGACAATCAGACTTCCACTATGATCAAGGCAACAGCAAGATCTGCACCA GATAGACAAGAGGAAATTAGCAGATTGGTAAGAAGTGCAAATTATGAAACAGATCCATTTGTTCAGGAGT TTCAATTTAAAGTTCGGGATGAAATGGCTCATGTAACTGGACGCGTACTTCCAGCACCTATGCTCCAGTA TGGAGGACGGAATCGGACAGTAGCAACACCGAGCCATGGAGTATGGGACATGCGAGGGAAACAATTCCAC ACAGGAGTTGAAATCAAAATGTGGGCTATCGCTTGTTTTGCCACACAGAGGCAGTGCAGAGAAGAAATAT TGAAGGGTTTCACAGACCAGCTGCGTAAGATTTCTAAGGATGCAGGGATGCCCATCCAGGGCCAGCCATG CTTCTGCAAATATGCACAGGGGGCAGACAGCGTAGAGCCCATGTTCCGGCATCTCAAGAACACATATTCT GGCCTACAGCTTATTATCGTCATCCTGCCGGGGAAGACACCAGTGTATGCGGAAGTGAAACGTGTAGGAG ACACACTTTTGGGTATGGCTACACAATGTGTTCAAGTCAAGAATGTAATAAAAACATCTCCTCAAACTCT GTCAAACTTGTGCCTAAAGATAAATGTTAAACTCGGAGGGATCAATAATATTCTTGTACCTCATCAAAGA CCTTCTGTGTTCCAGCAACCAGTGATCTTTTTGGGAGCCGATGTCACTCATCCACCTGCTGGTGATGGAA AGAAGCCTTCTATTGCTGCTGTTGTAGGTAGTATGGATGCACACCCAAGCAGATACTGTGCCACAGTAAG AGTTCAGAGACCCCGACAGGAGATCATCCAGGACTTGGCCTCCATGGTCCGGGAACTTCTTATTCAATTT TATAAGTCAACTCGGTTCAAGCCTACTCGTATCATCTTTTATCGGGATGGTGTTTCAGAGGGGCAGTTTA GGCAGGTATTATATTATGAACTACTAGCAATTCGAGAAGCCTGCATCAGTTTGGAGAAAGACTATCAACC TGGAATAACCTACATTGTAGTTCAGAAGAGACATCACACTCGATTATTTTGTGCTGATAGGACAGAAAGG GTTGGAAGAAGTGGCAATATCCCAGCTGGAACAACAGTTGATACAGACATTACACACCCATATGAGTTCG ATTTTTACCTCTGTAGCCATGCTGGAATACAGGGTACCAGTCGTCCTTCACACTATCATGTTTTATGGGA TGATAACTGCTTTACTGCAGATGAACTTCAGCTGCTAACTTACCAGCTCTGCCACACTTACGTACGCTGT ACACGATCTGTTTCTATACCTGCACCAGCGTATTATGCTCACCTGGTAGCATTTAGAGCCAGATATCATC TTGTGGACAAAGAACATGACAGTGCTGAAGGAAGTCACGTTTCAGGACAAAGCAATGGGCGAGATCCACA AGCTCTTGCCAAGGCTGTACAGATTCACCAAGATACCTTACGCACAATGTACTTCGCT AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA >RC211382 representing NM_177422
Red=Cloning site Green=Tags(s) MCEVLDIHNIDEQPRPLTDSHRVKFTKEIKGLKVEVTHCGTMRRKYRVCNVTRRPASHQTFPLQLENGQT VERTVAQYFREKYTLQLKYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDNQTSTMIKATARSAP DRQEEISRLVRSANYETDPFVQEFQFKVRDEMAHVTGRVLPAPMLQYGGRNRTVATPSHGVWDMRGKQFH TGVEIKMWAIACFATQRQCREEILKGFTDQLRKISKDAGMPIQGQPCFCKYAQGADSVEPMFRHLKNTYS GLQLIIVILPGKTPVYAEVKRVGDTLLGMATQCVQVKNVIKTSPQTLSNLCLKINVKLGGINNILVPHQR PSVFQQPVIFLGADVTHPPAGDGKKPSIAAVVGSMDAHPSRYCATVRVQRPRQEIIQDLASMVRELLIQF YKSTRFKPTRIIFYRDGVSEGQFRQVLYYELLAIREACISLEKDYQPGITYIVVQKRHHTRLFCADRTER VGRSGNIPAGTTVDTDITHPYEFDFYLCSHAGIQGTSRPSHYHVLWDDNCFTADELQLLTYQLCHTYVRC TRSVSIPAPAYYAHLVAFRARYHLVDKEHDSAEGSHVSGQSNGRDPQALAKAVQIHQDTLRTMYFA SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_177422 |
| ORF Size | 1878 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_177422.3 |
| RefSeq Size | 3320 bp |
| RefSeq ORF | 1881 bp |
| Locus ID | 192669 |
| UniProt ID | Q9H9G7 |
| Protein Families | Druggable Genome |
| MW | 71 kDa |
| Gene Summary | This gene encodes a member of the Argonaute family of proteins which play a role in RNA interference. The encoded protein is highly basic, contains a PAZ domain and a PIWI domain, and may play a role in short-interfering-RNA-mediated gene silencing. This gene is located on chromosome 1 in a tandem cluster of closely related family members including argonaute 4 and eukaryotic translation initiation factor 2C, 1. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC211382L3 | Lenti ORF clone of Human eukaryotic translation initiation factor 2C, 3 (EIF2C3), transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC211382L4 | Lenti ORF clone of Human eukaryotic translation initiation factor 2C, 3 (EIF2C3), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
| RG211382 | AGO3 (tGFP-tagged) - Human eukaryotic translation initiation factor 2C, 3 (EIF2C3), transcript variant 2 |
CNY 4,370.00 |
|
| SC307093 | AGO3 (untagged)-Human eukaryotic translation initiation factor 2C, 3 (EIF2C3), transcript variant 2 |
CNY 10,070.00 |
