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iNOS (NOS2) (NM_000625) Human Tagged ORF Clone
CAT＃: RC211819
NOS2 (Myc-DDK-tagged)-Human nitric oxide synthase 2, inducible (NOS2)
ORF Plasmid: tGFP
"NM_000625" in other vectors (6)
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CNY 12,040.00
| Cited in 4 publications. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | HEP-NOS; INOS; NOS; NOS2A |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC211819 representing NM_000625
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCTGTCCTTGGAAATTTCTGTTCAAGACCAAATTCCACCAGTATGCAATGAATGGGGAAAAAGACA TCAACAACAATGTGGAGAAAGCCCCCTGTGCCACCTCCAGTCCAGTGACACAGGATGACCTTCAGTATCA CAACCTCAGCAAGCAGCAGAATGAGTCCCCGCAGCCCCTCGTGGAGACGGGAAAGAAGTCTCCAGAATCT CTGGTCAAGCTGGATGCAACCCCATTGTCCTCCCCACGGCATGTGAGGATCAAAAACTGGGGCAGCGGGA TGACTTTCCAAGACACACTTCACCATAAGGCCAAAGGGATTTTAACTTGCAGGTCCAAATCTTGCCTGGG GTCCATTATGACTCCCAAAAGTTTGACCAGAGGACCCAGGGACAAGCCTACCCCTCCAGATGAGCTTCTA CCTCAAGCTATCGAATTTGTCAACAAATATTACGGCTCCTTCAAAGAGGCAAAAATAGAGGAACATCTGG CCAGGGTGGAAGCGGTAACAAAGGAGATAGAAACAACAGGAACCTACCAACTGACGGGAGATGAGCTCAT CTTCGCCACCAAGCAGGCCTGGCGCAATGCCCCACGCTGCATTGGGAGGATCCAGTGGTCCAACCTGCAG GTCTTCGATGCCCGCAGCTGTTCCACTGCCCGGGAAATGTTTGAACACATCTGCAGACACGTGCGTTACT CCACCAACAATGGCAACATCAGGTCGGCCATCACCGTGTTCCCCCAGCGGAGTGATGGCAAGCACGACTT CCGGGTGTGGAATGCTCAGCTCATCCGCTATGCTGGCTACCAGATGCCAGATGGCAGCATCAGAGGGGAC CCTGCCAACGTGGAATTCACTCAGCTGTGCATCGACCTGGGCTGGAAGCCCAAGTACGGCCGCTTCGATG TGGTCCCCCTGGTCCTGCAGGCCAATGGCCGTGACCCTGAGCTCTTCGAAATCCCACCTGACCTTGTGCT TGAGGTGGCCATGGAACATCCCAAATACGAGTGGTTTCGGGAACTGGAGCTAAAGTGGTACGCCCTGCCT GCAGTGGCCAACATGCTGCTTGAGGTGGGCGGCCTGGAGTTCCCAGGGTGCCCCTTCAATGGCTGGTACA TGGGCACAGAGATCGGAGTCCGGGACTTCTGTGACGTCCAGCGCTACAACATCCTGGAGGAAGTGGGCAG GAGAATGGGCCTGGAAACGCACAAGCTGGCCTCGCTCTGGAAAGACCAGGCTGTCGTTGAGATCAACATT GCTGTGCTCCATAGTTTCCAGAAGCAGAATGTGACCATCATGGACCACCACTCGGCTGCAGAATCCTTCA TGAAGTACATGCAGAATGAATACCGGTCCCGTGGGGGCTGCCCGGCAGACTGGATTTGGCTGGTCCCTCC CATGTCTGGGAGCATCACCCCCGTGTTTCACCAGGAGATGCTGAACTACGTCCTGTCCCCTTTCTACTAC TATCAGGTAGAGGCCTGGAAAACCCATGTCTGGCAGGACGAGAAGCGGAGACCCAAGAGAAGAGAGATTC CATTGAAAGTCTTGGTCAAAGCTGTGCTCTTTGCCTGTATGCTGATGCGCAAGACAATGGCGTCCCGAGT CAGAGTCACCATCCTCTTTGCGACAGAGACAGGAAAATCAGAGGCGCTGGCCTGGGACCTGGGGGCCTTA TTCAGCTGTGCCTTCAACCCCAAGGTTGTCTGCATGGATAAGTACAGGCTGAGCTGCCTGGAGGAGGAAC GGCTGCTGTTGGTGGTGACCAGTACGTTTGGCAATGGAGACTGCCCTGGCAATGGAGAGAAACTGAAGAA ATCGCTCTTCATGCTGAAAGAGCTCAACAACAAATTCAGGTACGCTGTGTTTGGCCTCGGCTCCAGCATG TACCCTCGGTTCTGCGCCTTTGCTCATGACATTGATCAGAAGCTGTCCCACCTGGGGGCCTCTCAGCTCA CCCCGATGGGAGAAGGGGATGAGCTCAGTGGGCAGGAGGACGCCTTCCGCAGCTGGGCCGTGCAAACCTT CAAGGCAGCCTGTGAGACGTTTGATGTCCGAGGCAAACAGCACATTCAGATCCCCAAGCTCTACACCTCC AATGTGACCTGGGACCCGCACCACTACAGGCTCGTGCAGGACTCACAGCCTTTGGACCTCAGCAAAGCCC TCAGCAGCATGCATGCCAAGAACGTGTTCACCATGAGGCTCAAATCTCGGCAGAATCTACAAAGTCCGAC ATCCAGCCGTGCCACCATCCTGGTGGAACTCTCCTGTGAGGATGGCCAAGGCCTGAACTACCTGCCGGGG GAGCACCTTGGGGTTTGCCCAGGCAACCAGCCGGCCCTGGTCCAAGGTATCCTGGAGCGAGTGGTGGATG GCCCCACACCCCACCAGACAGTGCGCCTGGAGGCCCTGGATGAGAGTGGCAGCTACTGGGTCAGTGACAA GAGGCTGCCCCCCTGCTCACTCAGCCAGGCCCTCACCTACTTCCTGGACATCACCACACCCCCAACCCAG CTGCTGCTCCAAAAGCTGGCCCAGGTGGCCACAGAAGAGCCTGAGAGACAGAGGCTGGAGGCCCTGTGCC AGCCCTCAGAGTACAGCAAGTGGAAGTTCACCAACAGCCCCACATTCCTGGAGGTGCTAGAGGAGTTCCC GTCCCTGCGGGTGTCTGCTGGCTTCCTGCTTTCCCAGCTCCCCATTCTGAAGCCCAGGTTCTACTCCATC AGCTCCTCCCGGGATCACATGCCCACAGAGATCCACCTGACTGTGGCCGTGGTCACCTACCACACCCGAG ATGGCCAGGGTCCCCTGCACCACGGCGTCTGCAGCACATGGCTCAACAGCCTGAAGCCCCAAGACCCAGT GCCCTGCTTTGTGCGGAATGCCAGCGGCTTCCACCTCCCCGAGGATCCCTCCCATCCTTGCATCCTCATC GGGCCTGGCACAGGCATCGCGCCCTTCCGCAGTTTCTGGCAGCAACGGCTCCATGACTCCCAGCACAAGG GAGTGCGGGGAGGCCGCATGACCTTGGTGTTTGGGTGCCGCCGCCCAGATGAGGACCACATCTACCAGGA GGAGATGCTGGAGATGGCCCAGAAGGGGGTGCTGCATGCGGTGCACACAGCCTATTCCCGCCTGCCTGGC AAGCCCAAGGTCTATGTTCAGGACATCCTGCGGCAGCAGCTGGCCAGCGAGGTGCTCCGTGTGCTCCACA AGGAGCCAGGCCACCTCTATGTTTGCGGGGATGTGCGCATGGCCCGGGACGTGGCCCACACCCTGAAGCA GCTGGTGGCTGCCAAGCTGAAATTGAATGAGGAGCAGGTCGAGGACTATTTCTTTCAGCTCAAGAGCCAG AAGCGCTATCACGAAGATATCTTTGGTGCTGTATTTCCTTACGAGGCGAAGAAGGACAGGGTGGCGGTGC AGCCCAGCAGCCTGGAGATGTCAGCGCTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC211819 representing NM_000625
Red=Cloning site Green=Tags(s) MACPWKFLFKTKFHQYAMNGEKDINNNVEKAPCATSSPVTQDDLQYHNLSKQQNESPQPLVETGKKSPES LVKLDATPLSSPRHVRIKNWGSGMTFQDTLHHKAKGILTCRSKSCLGSIMTPKSLTRGPRDKPTPPDELL PQAIEFVNKYYGSFKEAKIEEHLARVEAVTKEIETTGTYQLTGDELIFATKQAWRNAPRCIGRIQWSNLQ VFDARSCSTAREMFEHICRHVRYSTNNGNIRSAITVFPQRSDGKHDFRVWNAQLIRYAGYQMPDGSIRGD PANVEFTQLCIDLGWKPKYGRFDVVPLVLQANGRDPELFEIPPDLVLEVAMEHPKYEWFRELELKWYALP AVANMLLEVGGLEFPGCPFNGWYMGTEIGVRDFCDVQRYNILEEVGRRMGLETHKLASLWKDQAVVEINI AVLHSFQKQNVTIMDHHSAAESFMKYMQNEYRSRGGCPADWIWLVPPMSGSITPVFHQEMLNYVLSPFYY YQVEAWKTHVWQDEKRRPKRREIPLKVLVKAVLFACMLMRKTMASRVRVTILFATETGKSEALAWDLGAL FSCAFNPKVVCMDKYRLSCLEEERLLLVVTSTFGNGDCPGNGEKLKKSLFMLKELNNKFRYAVFGLGSSM YPRFCAFAHDIDQKLSHLGASQLTPMGEGDELSGQEDAFRSWAVQTFKAACETFDVRGKQHIQIPKLYTS NVTWDPHHYRLVQDSQPLDLSKALSSMHAKNVFTMRLKSRQNLQSPTSSRATILVELSCEDGQGLNYLPG EHLGVCPGNQPALVQGILERVVDGPTPHQTVRLEALDESGSYWVSDKRLPPCSLSQALTYFLDITTPPTQ LLLQKLAQVATEEPERQRLEALCQPSEYSKWKFTNSPTFLEVLEEFPSLRVSAGFLLSQLPILKPRFYSI SSSRDHMPTEIHLTVAVVTYHTRDGQGPLHHGVCSTWLNSLKPQDPVPCFVRNASGFHLPEDPSHPCILI GPGTGIAPFRSFWQQRLHDSQHKGVRGGRMTLVFGCRRPDEDHIYQEEMLEMAQKGVLHAVHTAYSRLPG KPKVYVQDILRQQLASEVLRVLHKEPGHLYVCGDVRMARDVAHTLKQLVAAKLKLNEEQVEDYFFQLKSQ KRYHEDIFGAVFPYEAKKDRVAVQPSSLEMSAL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_000625 |
| ORF Size | 3459 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_000625.4 |
| RefSeq Size | 4221 bp |
| RefSeq ORF | 3462 bp |
| Locus ID | 4843 |
| UniProt ID | P35228 |
| Protein Families | Druggable Genome |
| Protein Pathways | Alzheimer's disease, Amyotrophic lateral sclerosis (ALS), Arginine and proline metabolism, Calcium signaling pathway, Long-term depression, Metabolic pathways, Pathways in cancer, Small cell lung cancer |
| MW | 130.9 kDa |
| Gene Summary | Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. This gene encodes a nitric oxide synthase which is expressed in liver and is inducible by a combination of lipopolysaccharide and certain cytokines. Three related pseudogenes are located within the Smith-Magenis syndrome region on chromosome 17. [provided by RefSeq, Jul 2008] |
Citations (4)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Expression of inducible NOS is indispensable for the antiproliferative and proapoptotic effect of imatinib in BCR-ABL positive cells
,Singh, AK;Awasthi, D;Dubey, M;Nagarkoti, S;Chandra, T;Barthwal, MK;Tripathi, AK;Dikshit, M;,
Journal of leukocyte biology
,PubMed ID 33527482
[iNOS]
|
|
Pyroptotic and apoptotic cell death in iNOS and nNOS overexpressing K562 cells: a mechanistic insight
,Sadaf, S;Awasthi, D;Singh, AK;Nagarkoti, S;Kumar, S;Barthwal, MK;Dikshit, M;,
Biochem. Pharmacol.
,PubMed ID 31881190
[iNOS]
|
|
Augmentation of iNOS expression in myeloid progenitor cells expedites neutrophil differentiation
,Sadaf, S;Singh, AK;Awasthi, D;Nagarkoti, S;Agrahari, AK;Srivastava, RN;Jagavelu, K;Kumar, S;Barthwal, MK;Dikshit, M;,
J. Leukoc. Biol.
,PubMed ID 30861597
[iNOS]
|
|
Bipartite Vectors for co-expression of a Growth Factor cDNA and shRNA against an Apoptotic Gene
,null,
The journal of gene medicine
,PubMed ID 19554613
[iNOS]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC211819L1 | Lenti ORF clone of Human nitric oxide synthase 2, inducible (NOS2), Myc-DDK-tagged |
CNY 14,440.00 |
|
| RC211819L2 | Lenti ORF clone of Human nitric oxide synthase 2, inducible (NOS2), mGFP tagged |
CNY 13,400.00 |
|
| RC211819L3 | Lenti ORF clone of Human nitric oxide synthase 2, inducible (NOS2), Myc-DDK-tagged |
CNY 14,440.00 |
|
| RC211819L4 | Lenti ORF clone of Human nitric oxide synthase 2, inducible (NOS2), mGFP tagged |
CNY 14,440.00 |
|
| RG211819 | NOS2 (tGFP-tagged) - Human nitric oxide synthase 2, inducible (NOS2) |
CNY 13,640.00 |
|
| SC300111 | NOS2 (untagged)-Human nitric oxide synthase 2, inducible (NOS2) |
CNY 12,048.00 |
