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iNOS (NOS2) (NM_000625) Human Untagged Clone
CAT＃: SC300111
NOS2 (untagged)-Human nitric oxide synthase 2, inducible (NOS2)
CNY 12,048.00
| Cited in 2 publications. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | HEP-NOS; INOS; NOS; NOS2A |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene sequence for NM_000625 edited
CAGAAGCTCCTGTTTGTCCCGGCTTGAAGCTGTTTGCTGTGTGACCTTGCCCCACAGTGA AGAACATCTGAGCTCAAATCCAGATAAGTGACATAAGTGACCTGCTTTGTAAAGCCATAG AGATGGCCTGTCCTTGGAAATTTCTGTTCAAGACCAAATTCCACCAGTATGCAATGAATG GGGAAAAAGACATCAACAACAATGTGGAGAAAGCCCCCTGTGCCACCTCCAGTCCAGTGA CACAGGATGACCTTCAGTATCACAACCTCAGCAAGCAGCAGAATGAGTCCCCGCAGCCCC TCGTGGAGACGGGAAAGAAGTCTCCAGAATCTCTGGTCAAGCTGGATGCAACCCCATTGT CCTCCCCACGGCATGTGAGGATCAAAAACTGGGGCAGCGGGATGACTTTCCAAGACACAC TTCACCATAAGGCCAAAGGGATTTTAACTTGCAGGTCCAAATCTTGCCTGGGGTCCATTA TGACTCCCAAAAGTTTGACCAGAGGACCCAGGGACAAGCCTACCCCTCCAGATGAGCTTC TACCTCAAGCTATCGAATTTGTCAACAAATATTACGGCTCCTTCAAAGAGGCAAAAATAG AGGAACATCTGGCCAGGGTGGAAGCGGTAACAAAGGAGATAGAAACAACAGGAACCTACC AACTGACGGGAGATGAGCTCATCTTCGCCACCAAGCAGGCCTGGCGCAATGCCCCACGCT GCATTGGGAGGATCCAGTGGTCCAACCTGCAGGTCTTCGATGCCCGCAGCTGTTCCACTG CCCGGGAAATGTTTGAACACATCTGCAGACACGTGCGTTACTCCACCAACAATGGCAACA TCAGGTCGGCCATCACCGTGTTCCCCCAGCGGAGTGATGGCAAGCACGACTTCCGGGTGT GGAATGCTCAGCTCATCCGCTATGCTGGCTACCAGATGCCAGATGGCAGCATCAGAGGGG ACCCTGCCAACGTGGAATTCACTCAGCTGTGCATCGACCTGGGCTGGAAGCCCAAGTACG GCCGCTTCGATGTGGTCCCCCTGGTCCTGCAGGCCAATGGCCGTGACCCTGAGCTCTTCG AAATCCCACCTGACCTTGTGCTTGAGGTGGCCATGGAACATCCCAAATACGAGTGGTTTC GGGAACTGGAGCTAAAGTGGTACGCCCTGCCTGCAGTGGCCAACATGCTGCTTGAGGTGG GCGGCCTGGAGTTCCCAGGGTGCCCCTTCAATGGCTGGTACATGGGCACAGAGATCGGAG TCCGGGACTTCTGTGACGTCCAGCGCTACAACATCCTGGAGGAAGTGGGCAGGAGAATGG GCCTGGAAACGCACAAGCTGGCCTCGCTCTGGAAAGACCAGGCTGTCGTTGAGATCAACA TTGCTGTGCTCCATAGTTTCCAGAAGCAGAATGTGACCATCATGGACCACCACTCGGCTG CAGAATCCTTCATGAAGTACATGCAGAATGAATACCGGTCCCGTGGGGGCTGCCCGGCAG ACTGGATTTGGCTGGTCCCTCCCATGTCTGGGAGCATCACCCCCGTGTTTCACCAGGAGA TGCTGAACTACGTCCTGTCCCCTTTCTACTACTATCAGGTAGAGGCCTGGAAAACCCATG TCTGGCAGGACGAGAAGCGGAGACCCAAGAGAAGAGAGATTCCATTGAAAGTCTTGGTCA AAGCTGTGCTCTTTGCCTGTATGCTGATGCGCAAGACAATGGCGTCCCGAGTCAGAGTCA CCATCCTCTTTGCGACAGAGACAGGAAAATCAGAGGCGCTGGCCTGGGACCTGGGGGCCT TATTCAGCTGTGCCTTCAACCCCAAGGTTGTCTGCATGGATAAGTACAGGCTGAGCTGCC TGGAGGAGGAACGGCTGCTGTTGGTGGTGACCAGTACGTTTGGCAATGGAGACTGCCCTG GCAATGGAGAGAAACTGAAGAAATCGCTCTTCATGCTGAAAGAGCTCAACAACAAATTCA GGTACGCTGTGTTTGGCCTCGGCTCCAGCATGTACCCTCGGTTCTGCGCCTTTGCTCATG ACATTGATCAGAAGCTGTCCCACCTGGGGGCCTCTCAGCTCACCCCGATGGGAGAAGGGG ATGAGCTCAGTGGGCAGGAGGACGCCTTCCGCAGCTGGGCCGTGCAAACCTTCAAGGCAG CCTGTGAGACGTTTGATGTCCGAGGCAAACAGCACATTCAGATCCCCAAGCTCTACACCT CCAATGTGACCTGGGACCCGCACCACTACAGGCTCGTGCAGGACTCACAGCCTTTGGACC TCAGCAAAGCCCTCAGCAGCATGCATGCCAAGAACGTGTTCACCATGAGGCTCAAATCTC GGCAGAATCTACAAAGTCCGACATCCAGCCGTGCCACCATCCTGGTGGAACTCTCCTGTG AGGATGGCCAAGGCCTGAACTACCTGCCGGGGGAGCACCTTGGGGTTTGCCCAGGCAACC AGCCGGCCCTGGTCCAAGGTATCCTGGAGCGAGTGGTGGATGGCCCCACACCCCACCAGA CAGTGCGCCTGGAGGCCCTGGATGAGAGTGGCAGCTACTGGGTCAGTGACAAGAGGCTGC CCCCCTGCTCACTCAGCCAGGCCCTCACCTACTTCCTGGACATCACCACACCCCCAACCC AGCTGCTGCTCCAAAAGCTGGCCCAGGTGGCCACAGAAGAGCCTGAGAGACAGAGGCTGG AGGCCCTGTGCCAGCCCTCAGAGTACAGCAAGTGGAAGTTCACCAACAGCCCCACATTCC TGGAGGTGCTAGAGGAGTTCCCGTCCCTGCGGGTGTCTGCTGGCTTCCTGCTTTCCCAGC TCCCCATTCTGAAGCCCAGGTTCTACTCCATCAGCTCCTCCCGGGATCACATGCCCACAG AGATCCACCTGACTGTGGCCGTGGTCACCTACCACACCCGAGATGGCCAGGGTCCCCTGC ACCACGGCGTCTGCAGCACATGGCTCAACAGCCTGAAGCCCCAAGACCCAGTGCCCTGCT TTGTGCGGAATGCCAGCGGCTTCCACCTCCCCGAGGATCCCTCCCATCCTTGCATCCTCA TCGGGCCTGGCACAGGCATCGCGCCCTTCCGCAGTTTCTGGCAGCAACGGCTCCATGACT CCCAGCACAAGGGAGTGCGGGGAGGCCGCATGACCTTGGTGTTTGGGTGCCGCCGCCCAG ATGAGGACCACATCTACCAGGAGGAGATGCTGGAGATGGCCCAGAAGGGGGTGCTGCATG CGGTGCACACAGCCTATTCCCGCCTGCCTGGCAAGCCCAAGGTCTATGTTCAGGACATCC TGCGGCAGCAGCTGGCCAGCGAGGTGCTCCGTGTGCTCCACAAGGAGCCAGGCCACCTCT ATGTTTGCGGGGATGTGCGCATGGCCCGGGACGTGGCCCACACCCTGAAGCAGCTGGTGG CTGCCAAGCTGAAATTGAATGAGGAGCAGGTCGAGGACTATTTCTTTCAGCTCAAGAGCC AGAAGCGCTATCACGAAGATATCTTTGGTGCTGTATTTCCTTACGAGGCGAAGAAGGACA GGGTGGCGGTGCAGCCCAGCAGCCTGGAGATGTCAGCGCTCTGAGGGCCTACAGGAGGGG TTAAAGCTGCCGGCACAGAACTTAAGGATGGAGCCAGCTCTGCATTATCTGAGGTCACAG GGCCTGGGGAGATGGAGGAAAGTGATATCCCCCAGCCTCAAGTCTTATTTCCTCAACGTT GCTCCCCATCAAGCCCTTTACTTGACCTCCTAACAAGTAGCACCCTGGATTGATCGGAGC CTCCTCTCTCAAACTGGGGCCTCCCTGGTCCCTTGGAGACAAAATCTTAAATGCCAGGCC TGGCAAGTGGGTGAAAGATGGAACTTGCTGCTGAGTGCACCACTTCAAGTGACCACCAGG AGGTGCTATCGCACCACTGTGTATTTAACTGCCTTGTGTACAGTTATTTATGCCTCTGTA TTTAAAAAACTAACACCCAGTCTGTTCCCCATGGCCACTTGGGTCTTCCCTGTATGATTC CTTGATGGAGATATTTACATGAATTGCATTTTACTTTAATCACAAAAAAAAAAAAAAAAA A >OriGene 5' read for NM_000625 unedited
GGGGAGGATCTGCCATTTTGTATACNACTCACTATTAGGGCGGCCGCGTAATTCGCACGA CANAACTCCTGTTTGTCCCGGCTTGAAGCTGTTTGCTGTGTGACCTTGCCCCACAGTGAA GAACATCTGAGCTCAAATCCAGATAAGTGACATAAGTGACCTGCTTTGTAAAGCCATAGA GATGGCCTGTCCTTGGAAATTTCTGTTCAAGACCAAATTCCACCAGTATGCAATGAATGG GGAAAAAGACATCAACAACAATGTGGAGAAAGCCCCCTGTGCCACCTCCAGTCCAGTGAC ACAGGATGACCTTCAGTATCACAACCTCAGCAAGCAGCAGAATGAGTCCCCGCAGCCCCT CGTGGAGACGGGAAAGAAGTCTCCAGAATCTCTGGTCAAGCTGGATGCAACCCCATTGTC CTCCCCACGGCATGTGAGGATCAAAAACTGGGGCAGCGGGATGACTTTCCAAGACACACT TCACCATAAGGCCAAAGGGATTTTAACTTGCAGGTCCAAATCTTGCCTGGGGTCCATTAT GACTCCCAAAAGTTTGACCAGAGGACCCAGGGACAAGCCTACNCCTCCAGATGAGCTTCT ACCTCAAGCTATCGAATTTGTCAACAAATATTACGGCTCCTTCAAAGAGGCAAAAATAGA GGAACATCTGGCCCAGGGTGGAAAGCGTAACCAAGGAGATAGAAACAACGGAACCTACCC ACTGACGGGAGATGAGCTCATCTTCGCCACCAAGCAGGCCTGGCGCAATGCCCCACGCTG CATTGGGAGGATCCAGTGGGTCCACCCTGCAGTCTCCGATGCCCCAGCTGTTCCACTGTC CGGGAAATGTTTGAACCCTTTGCCGACACTGCGTTACTCCCCAACCATGGCAACTTCAGT CCGGCCTT >OriGene 3' read for NM_000625 unedited
NGCCCCCATTGGGNNGATGGCAACTTNCCAGGTCCAGNAAAGCACTGGGGNAGGGTCACA GGNATGCCACCCGGGCTCTGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTAGAGTCG AGTTTTTTTTTTTTTTTTTTGTGATTAAAGTAAAATGCAATTCATGTAAATATCTCCATC AAGGAATCATACAGGGGAAGACCCAAGTGGCCATGGGGAACAGACTGGGTGTTAGTTTTT TAAATACAGAGGCATAAATAACTGTACACAAGGCAGTTAAATACACAGTGGTGCGATAGC ACCTCCTGGTGGTCACTTGAAGTGGTGCACTCAGCAGCAAGTTCCATCTTTCACCCACTT GCCAGGCCTGGCATTTAAGATTTTGTCTCCAAGGGACCAGGGAGGCCCCAGTTTGAGAGA GGAGGCTCCGATCAATCCAGGGTGCTACTTGTTAGGAGGTCAAGTAAAGGGCTTGATGGG GAGCAACGTTGAGGAAATAAGACTTGAGGCTGGGGGATATCACTTTCCTCCATCTCCCCA GGCCCTGTGACCTCAGATAATGCAGAGCTGGCTCCATCCTTAAGTTCTGTGCCCGCAGCT TTAACCCCTCCTGTANGCCCTCAGAGCGCTGACATCTCCAGGCTGCTGGGCTGCACCGNC ACCCTGTCCTTCTTCGCCTCGTAAGGAAATACAGCACCAAAGATATCTTCGTGATAGCGC TCCTGGCTCTTGGACTGAAAGAAATAGTCCTCGACCTGCTCCTCATTCATTTCAGCTTGG CAGCCACCAGCTGCTTCAGGGGTGTGGGCCACGTTCCGGGCCATGCCCACCATCCCGGAA AACATAGAAGTTGCCTGGCTTCTTGTGGAACACAAGGACCACTTGCTTGGCCACTGCTGC CGAAGGAAGTCCTGAAACTAAACCTGGN |
| Restriction Sites | NotI-NotI |
| ACCN | NM_000625 |
| Insert Size | 4100 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | There is 2 nucleotide difference between the OriGene clone and the NCBI reference ORF. These result in the substitution of 2 aa. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_000625.3, NP_000616.3 |
| RefSeq Size | 4221 bp |
| RefSeq ORF | 3462 bp |
| Locus ID | 4843 |
| UniProt ID | P35228 |
| Protein Families | Druggable Genome |
| Protein Pathways | Alzheimer's disease, Amyotrophic lateral sclerosis (ALS), Arginine and proline metabolism, Calcium signaling pathway, Long-term depression, Metabolic pathways, Pathways in cancer, Small cell lung cancer |
| Gene Summary | Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. This gene encodes a nitric oxide synthase which is expressed in liver and is inducible by a combination of lipopolysaccharide and certain cytokines. Three related pseudogenes are located within the Smith-Magenis syndrome region on chromosome 17. [provided by RefSeq, Jul 2008] |
Citations (2)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Microsomal PGE2 synthase-1 regulates melanoma cell survival and associates with melanoma disease progression
,Kim, SH;Hashimoto, Y;Cho, SN;Roszik, J;Milton, DR;Dal, F;Kim, SF;Menter, DG;Yang, P;Ekmekcioglu, S;Grimm, EA;,
Pigment Cell Melanoma Res
,PubMed ID 26801201
[NOS2]
|
|
C. pneumoniae disrupts eNOS trafficking and impairs NO production in human aortic endothelial cells
,Mueller, KE;Wolf, K;,
Cell. Microbiol.
,PubMed ID 25131610
[NOS2]
|
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC211819 | NOS2 (Myc-DDK-tagged)-Human nitric oxide synthase 2, inducible (NOS2) |
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| RC211819L1 | Lenti ORF clone of Human nitric oxide synthase 2, inducible (NOS2), Myc-DDK-tagged |
CNY 14,440.00 |
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| RC211819L2 | Lenti ORF clone of Human nitric oxide synthase 2, inducible (NOS2), mGFP tagged |
CNY 13,400.00 |
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| RC211819L3 | Lenti ORF clone of Human nitric oxide synthase 2, inducible (NOS2), Myc-DDK-tagged |
CNY 14,440.00 |
|
| RC211819L4 | Lenti ORF clone of Human nitric oxide synthase 2, inducible (NOS2), mGFP tagged |
CNY 14,440.00 |
|
| RG211819 | NOS2 (tGFP-tagged) - Human nitric oxide synthase 2, inducible (NOS2) |
CNY 13,640.00 |
