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Nudel (NDEL1) (NM_001025579) Human Tagged ORF Clone
CAT＃: RC212323
NDEL1 (Myc-DDK-tagged)-Human nudE nuclear distribution gene E homolog (A. nidulans)-like 1 (NDEL1), transcript variant 1
ORF Plasmid: tGFP
"NM_001025579" in other vectors (4)
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CNY 2,400.00
CNY 3,990.00
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | EOPA; MITAP1; NDE1L1; NDE2; NUDEL |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC212323 representing NM_001025579
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATGGTGAAGATATACCAGATTTTTCAAGTTTAAAGGAGGAAACTGCTTATTGGAAGGAACTTTCCT TGAAGTATAAGCAAAGCTTCCAGGAAGCTCGGGATGAGCTAGTTGAATTCCAGGAAGGAAGCAGAGAATT AGAAGCAGAGTTGGAGGCACAATTAGTACAGGCTGAACAAAGAAATAGAGACTTGCAGGCTGATAACCAA AGACTGAAATATGAAGTGGAGGCATTAAAGGAGAAGCTAGAGCATCAATATGCACAGAGCTATAAGCAGG TCTCAGTGTTAGAAGATGATTTAAGTCAGACTCGGGCCATTAAGGAGCAGTTGCATAAGTATGTGAGAGA GCTGGAGCAGGCCAACGACGACCTGGAGCGAGCCAAAAGGGCAACAATAGTTTCACTGGAAGACTTTGAA CAAAGGCTAAACCAGGCCATTGAACGAAATGCATTTTTAGAAAGTGAACTTGATGAAAAGGAATCTTTGT TGGTCTCTGTACAGAGGTTAAAGGATGAAGCAAGAGATTTAAGGCAAGAACTAGCAGTTCGGGAAAGACA ACAGGAAGTAACTAGAAAGTCGGCTCCTAGCTCTCCAACTCTAGACTGTGAAAAGATGGACTCCGCCGTC CAAGCATCACTTTCTTTGCCAGCTACCCCTGTTGGCAAAGGAACGGAGAACACTTTTCCTTCACCGAAAG CTATACCAAATGGTTTTGGTACCAGTCCACTAACTCCCTCTGCTAGGATATCAGCACTAAACATCGTGGG GGATCTCTTACGGAAAGTAGGGGCTTTAGAATCCAAATTAGCAGCTTGCAGGAATTTTGCAAAGGACCAA GCATCACGAAAATCCTATATTTCAGGGAATGTTAACTGTGGGGTGCTGAATGGCAATGGCACAAAGTTCT CTCGATCAGGGCATACATCTTTCTTCGACAAAGGGCAAGAAAAAGTCATATTTCCCACGTTGTTCATGGG GCAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC212323 representing NM_001025579
Red=Cloning site Green=Tags(s) MDGEDIPDFSSLKEETAYWKELSLKYKQSFQEARDELVEFQEGSRELEAELEAQLVQAEQRNRDLQADNQ RLKYEVEALKEKLEHQYAQSYKQVSVLEDDLSQTRAIKEQLHKYVRELEQANDDLERAKRATIVSLEDFE QRLNQAIERNAFLESELDEKESLLVSVQRLKDEARDLRQELAVRERQQEVTRKSAPSSPTLDCEKMDSAV QASLSLPATPVGKGTENTFPSPKAIPNGFGTSPLTPSARISALNIVGDLLRKVGALESKLAACRNFAKDQ ASRKSYISGNVNCGVLNGNGTKFSRSGHTSFFDKGQEKVIFPTLFMGQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001025579 |
| ORF Size | 984 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001025579.3 |
| RefSeq Size | 2420 bp |
| RefSeq ORF | 987 bp |
| Locus ID | 81565 |
| UniProt ID | Q9GZM8 |
| MW | 36.9 kDa |
| Gene Summary | This gene encodes a coiled-coil protein that plays a role in multiple processes including cytoskeletal organization, cell signaling and neuron migration, outgrowth and maintenance. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome X. [provided by RefSeq, Mar 2012] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC212323L3 | Lenti ORF clone of Human nudE nuclear distribution gene E homolog (A. nidulans)-like 1 (NDEL1), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC212323L4 | Lenti ORF clone of Human nudE nuclear distribution gene E homolog (A. nidulans)-like 1 (NDEL1), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RG212323 | NDEL1 (tGFP-tagged) - Human nudE nuclear distribution gene E homolog (A. nidulans)-like 1 (NDEL1), transcript variant 1 |
CNY 4,000.00 |
|
| SC302418 | NDEL1 (untagged)-Human nudE nuclear distribution gene E homolog (A. nidulans)-like 1 (NDEL1), transcript variant 1 |
CNY 2,400.00 |
