ADAT2 (NM_182503) Human Tagged ORF Clone
CAT#: RC212395
ADAT2 (Myc-DDK-tagged)-Human adenosine deaminase, tRNA-specific 2 (ADAT2)
ORF Plasmid: tGFP
"NM_182503" in other vectors (4)
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CNY 2,400.00
CNY 3,705.00
Cited in 2 publications. |
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | DEADC1; dJ20N2; dJ20N2.1; TAD2 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC212395 representing NM_182503
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGCGAAGGCGGCACCCAAGCCAGCTGCAAGCGGCGCGTGCTCGGTGTCGGCAGAGGAGACCGAAA AGTGGATGGAGGAGGCGATGCACATGGCCAAAGAAGCCCTCGAAAATACTGAAGTTCCTGTTGGCTGTCT TATGGTCTACAACAATGAAGTTGTAGGGAAGGGGAGAAATGAAGTTAACCAAACCAAAAATGCTACTCGA CATGCAGAAATGGTGGCCATCGATCAGGTCCTCGATTGGTGTCGTCAAAGTGGCAAGAGTCCCTCTGAAG TATTTGAACACACTGTGTTGTATGTCACTGTGGAGCCGTGCATTATGTGTGCAGCTGCTCTCCGCCTGAT GAAAATCCCGCTGGTTGTATATGGCTGTCAGAATGAACGATTTGGTGGTTGTGGCTCTGTTCTAAATATT GCCTCTGCTGACCTACCAAACACTGGGAGACCATTTCAGTGTATCCCTGGATATCGGGCTGAGGAAGCAG TGGAAATGTTAAAGACCTTCTACAAACAAGAAAATCCAAATGCACCAAAATCGAAAGTTCGGAAAAAGGA ATGTCAGAAATCT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC212395 representing NM_182503
Red=Cloning site Green=Tags(s) MEAKAAPKPAASGACSVSAEETEKWMEEAMHMAKEALENTEVPVGCLMVYNNEVVGKGRNEVNQTKNATR HAEMVAIDQVLDWCRQSGKSPSEVFEHTVLYVTVEPCIMCAAALRLMKIPLVVYGCQNERFGGCGSVLNI ASADLPNTGRPFQCIPGYRAEEAVEMLKTFYKQENPNAPKSKVRKKECQKS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_182503 |
ORF Size | 573 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_182503.3 |
RefSeq Size | 6270 bp |
RefSeq ORF | 576 bp |
Locus ID | 134637 |
UniProt ID | Q7Z6V5 |
MW | 20.9 kDa |
Gene Summary | Probably participates in deamination of adenosine-34 to inosine in many tRNAs.[UniProtKB/Swiss-Prot Function] |
Citations (2)
The use of this cDNA Clones has been cited in the following citations: |
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Formation of tRNA Wobble Inosine in Humans Is Disrupted by a Millennia-Old Mutation Causing Intellectual Disability
,Ramos, J;Han, L;Li, Y;Hagelskamp, F;Kellner, SM;Alkuraya, FS;Phizicky, EM;Fu, D;,
Mol. Cell. Biol.
,PubMed ID 31263000
[ADAT2]
|
Formation of tRNA wobble inosine in humans is perturbed by a millennia-old mutation linked to intellectual disability
,Ramos, J;Han, L;Li, Y;Alkuraya, F;Phizicky, E;Fu, D;,
bioRxiv
[ADAT2]
|
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC212395L3 | Lenti ORF clone of Human adenosine deaminase, tRNA-specific 2 (ADAT2), Myc-DDK-tagged |
CNY 5,890.00 |
|
RC212395L4 | Lenti ORF clone of Human adenosine deaminase, tRNA-specific 2 (ADAT2), mGFP tagged |
CNY 5,890.00 |
|
RG212395 | ADAT2 (tGFP-tagged) - Human adenosine deaminase, tRNA-specific 2 (ADAT2) |
CNY 4,370.00 |
|
SC317284 | ADAT2 (untagged)-Human adenosine deaminase, tRNA-specific 2 (ADAT2) |
CNY 3,990.00 |