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LGR5 (NM_003667) Human Tagged ORF Clone
CAT＃: RC212825
LGR5 (Myc-DDK-tagged)-Human leucine-rich repeat containing G protein-coupled receptor 5 (LGR5)
ORF Plasmid: tGFP
"NM_003667" in other vectors (6)
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CNY 9,960.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | FEX; GPR49; GPR67; GRP49; HG38 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC212825 representing NM_003667
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACACCTCCCGGCTCGGTGTGCTCCTGTCCTTGCCTGTGCTGCTGCAGCTGGCGACCGGGGGCAGCT CTCCCAGGTCTGGTGTGTTGCTGAGGGGCTGCCCCACACACTGTCATTGCGAGCCCGACGGCAGGATGTT GCTCAGGGTGGACTGCTCCGACCTGGGGCTCTCGGAGCTGCCTTCCAACCTCAGCGTCTTCACCTCCTAC CTAGACCTCAGTATGAACAACATCAGTCAGCTGCTCCCGAATCCCCTGCCCAGTCTCCGCTTCCTGGAGG AGTTACGTCTTGCGGGAAACGCTCTGACATACATTCCCAAGGGAGCATTCACTGGCCTTTACAGTCTTAA AGTTCTTATGCTGCAGAATAATCAGCTAAGACACGTACCCACAGAAGCTCTGCAGAATTTGCGAAGCCTT CAATCCCTGCGTCTGGATGCTAACCACATCAGCTATGTGCCCCCAAGCTGTTTCAGTGGCCTGCATTCCC TGAGGCACCTGTGGCTGGATGACAATGCGTTAACAGAAATCCCCGTCCAGGCTTTTAGAAGTTTATCGGC ATTGCAAGCCATGACCTTGGCCCTGAACAAAATACACCACATACCAGACTATGCCTTTGGAAACCTCTCC AGCTTGGTAGTTCTACATCTCCATAACAATAGAATCCACTCCCTGGGAAAGAAATGCTTTGATGGGCTCC ACAGCCTAGAGACTTTAGATTTAAATTACAATAACCTTGATGAATTCCCCACTGCAATTAGGACACTCTC CAACCTTAAAGAACTAGGATTTCATAGCAACAATATCAGGTCGATACCTGAGAAAGCATTTGTAGGCAAC CCTTCTCTTATTACAATACATTTCTATGACAATCCCATCCAATTTGTTGGGAGATCTGCTTTTCAACATT TACCTGAACTAAGAACACTGACTCTGAATGGTGCCTCACAAATAACTGAATTTCCTGATTTAACTGGAAC TGCAAACCTGGAGAGTCTGACTTTAACTGGAGCACAGATCTCATCTCTTCCTCAAACCGTCTGCAATCAG TTACCTAATCTCCAAGTGCTAGATCTGTCTTACAACCTATTAGAAGATTTACCCAGTTTTTCAGTCTGCC AAAAGCTTCAGAAAATTGACCTAAGACATAATGAAATCTACGAAATTAAAGTTGACACTTTCCAGCAGTT GCTTAGCCTCCGATCGCTGAATTTGGCTTGGAACAAAATTGCTATTATTCACCCCAATGCATTTTCCACT TTGCCATCCCTAATAAAGCTGGACCTATCGTCCAACCTCCTGTCGTCTTTTCCTATAACTGGGTTACATG GTTTAACTCACTTAAAATTAACAGGAAATCATGCCTTACAGAGCTTGATATCATCTGAAAACTTTCCAGA ACTCAAGGTTATAGAAATGCCTTATGCTTACCAGTGCTGTGCATTTGGAGTGTGTGAGAATGCCTATAAG ATTTCTAATCAATGGAATAAAGGTGACAACAGCAGTATGGACGACCTTCATAAGAAAGATGCTGGAATGT TTCAGGCTCAAGATGAACGTGACCTTGAAGATTTCCTGCTTGACTTTGAGGAAGACCTGAAAGCCCTTCA TTCAGTGCAGTGTTCACCTTCCCCAGGCCCCTTCAAACCCTGTGAACACCTGCTTGATGGCTGGCTGATC AGAATTGGAGTGTGGACCATAGCAGTTCTGGCACTTACTTGTAATGCTTTGGTGACTTCAACAGTTTTCA GATCCCCTCTGTACATTTCCCCCATTAAACTGTTAATTGGGGTCATCGCAGCAGTGAACATGCTCACGGG AGTCTCCAGTGCCGTGCTGGCTGGTGTGGATGCGTTCACTTTTGGCAGCTTTGCACGACATGGTGCCTGG TGGGAGAATGGGGTTGGTTGCCATGTCATTGGTTTTTTGTCCATTTTTGCTTCAGAATCATCTGTTTTCC TGCTTACTCTGGCAGCCCTGGAGCGTGGGTTCTCTGCGAAATATTCTGCAAAATTTGAAACGAAAGCTCC ATTTTCTAGCCTGAAAGTAATCATTTTGCTCTGTGCCCTGCTGGCCTTGACCATGGCCGCAGTTCCCCTG CTGGGTGGCAGCAAGTATGGCGCCTCCCCTCTCTGCCTGCCTTTGCCTTTTGGGGAGCCCAGCACCATGG GCTACATGGTCGCTCTCATCTTGCTCAATTCCCTTTGCTTCCTCATGATGACCATTGCCTACACCAAGCT CTACTGCAATTTGGACAAGGGAGACCTGGAGAATATTTGGGACTGCTCTATGGTAAAACACATTGCCCTG TTGCTCTTCACCAACTGCATCCTAAACTGCCCTGTGGCTTTCTTGTCCTTCTCCTCTTTAATAAACCTTA CATTTATCAGTCCTGAAGTAATTAAGTTTATCCTTCTGGTGGTAGTCCCACTTCCTGCATGTCTCAATCC CCTTCTCTACATCTTGTTCAATCCTCACTTTAAGGAGGATCTGGTGAGCCTGAGAAAGCAAACCTACGTC TGGACAAGATCAAAACACCCAAGCTTGATGTCAATTAACTCTGATGATGTCGAAAAACAGTCCTGTGACT CAACTCAAGCCTTGGTAACCTTTACCAGCTCCAGCATCACTTATGACCTGCCTCCCAGTTCCGTGCCATC ACCAGCTTATCCAGTGACTGAGAGCTGCCATCTTTCCTCTGTGGCATTTGTCCCATGTCTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC212825 representing NM_003667
Red=Cloning site Green=Tags(s) MDTSRLGVLLSLPVLLQLATGGSSPRSGVLLRGCPTHCHCEPDGRMLLRVDCSDLGLSELPSNLSVFTSY LDLSMNNISQLLPNPLPSLRFLEELRLAGNALTYIPKGAFTGLYSLKVLMLQNNQLRHVPTEALQNLRSL QSLRLDANHISYVPPSCFSGLHSLRHLWLDDNALTEIPVQAFRSLSALQAMTLALNKIHHIPDYAFGNLS SLVVLHLHNNRIHSLGKKCFDGLHSLETLDLNYNNLDEFPTAIRTLSNLKELGFHSNNIRSIPEKAFVGN PSLITIHFYDNPIQFVGRSAFQHLPELRTLTLNGASQITEFPDLTGTANLESLTLTGAQISSLPQTVCNQ LPNLQVLDLSYNLLEDLPSFSVCQKLQKIDLRHNEIYEIKVDTFQQLLSLRSLNLAWNKIAIIHPNAFST LPSLIKLDLSSNLLSSFPITGLHGLTHLKLTGNHALQSLISSENFPELKVIEMPYAYQCCAFGVCENAYK ISNQWNKGDNSSMDDLHKKDAGMFQAQDERDLEDFLLDFEEDLKALHSVQCSPSPGPFKPCEHLLDGWLI RIGVWTIAVLALTCNALVTSTVFRSPLYISPIKLLIGVIAAVNMLTGVSSAVLAGVDAFTFGSFARHGAW WENGVGCHVIGFLSIFASESSVFLLTLAALERGFSAKYSAKFETKAPFSSLKVIILLCALLALTMAAVPL LGGSKYGASPLCLPLPFGEPSTMGYMVALILLNSLCFLMMTIAYTKLYCNLDKGDLENIWDCSMVKHIAL LLFTNCILNCPVAFLSFSSLINLTFISPEVIKFILLVVVPLPACLNPLLYILFNPHFKEDLVSLRKQTYV WTRSKHPSLMSINSDDVEKQSCDSTQALVTFTSSSITYDLPPSSVPSPAYPVTESCHLSSVAFVPCL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_003667 |
| ORF Size | 2721 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_003667.4 |
| RefSeq Size | 2880 bp |
| RefSeq ORF | 2724 bp |
| Locus ID | 8549 |
| UniProt ID | O75473 |
| Domains | 7tm_1, LRRNT, LRR, LRR_TYP, LRR_PS |
| Protein Families | Druggable Genome, GPCR, Transmembrane |
| MW | 99.8 kDa |
| Gene Summary | The protein encoded by this gene is a leucine-rich repeat-containing receptor (LGR) and member of the G protein-coupled, 7-transmembrane receptor (GPCR) superfamily. The encoded protein is a receptor for R-spondins and is involved in the canonical Wnt signaling pathway. This protein plays a role in the formation and maintenance of adult intestinal stem cells during postembryonic development. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2015] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Depletion of death-associated protein-3 induces chemoresistance in gastric cancer cells through the β-catenin/LGR5/Bcl-2 axis
,Jia, Y;Li, Z;Cheng, X;Wu, X;Pang, F;Shi, J;Li, S;Li, X;Hu, Y;Zhang, L;Ji, J;,
J. Investig. Med.
,PubMed ID 30792218
[LGR5]
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Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC212825L1 | Lenti ORF clone of Human leucine-rich repeat containing G protein-coupled receptor 5 (LGR5), Myc-DDK-tagged |
CNY 12,360.00 |
|
| RC212825L2 | Lenti ORF clone of Human leucine-rich repeat containing G protein-coupled receptor 5 (LGR5), mGFP tagged |
CNY 5,890.00 |
|
| RC212825L3 | Lenti ORF clone of Human leucine-rich repeat containing G protein-coupled receptor 5 (LGR5), Myc-DDK-tagged |
CNY 12,360.00 |
|
| RC212825L4 | Lenti ORF clone of Human leucine-rich repeat containing G protein-coupled receptor 5 (LGR5), mGFP tagged |
CNY 12,360.00 |
|
| RG212825 | LGR5 (tGFP-tagged) - Human leucine-rich repeat containing G protein-coupled receptor 5 (LGR5) |
CNY 11,560.00 |
|
| SC117852 | LGR5 (untagged)-Human leucine-rich repeat containing G protein-coupled receptor 5 (LGR5) |
CNY 9,976.00 |
