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LGR5 (NM_003667) Human Untagged Clone
CAT＃: SC117852
LGR5 (untagged)-Human leucine-rich repeat containing G protein-coupled receptor 5 (LGR5)
CNY 9,976.00
| Cited in 3 publications. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | FEX; GPR49; GPR67; GRP49; HG38 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC117852 sequence for NM_003667 edited (data generated by NextGen Sequencing)
ATGGACACCTCCCGGCTCGGTGTGCTCCTGTCCTTGCCTGTGCTGCTGCAGCTGGCGACC GGGGGCAGCTCTCCCAGGTCTGGTGTGTTGCTGAGGGGCTGCCCCACACACTGTCATTGC GAGCCCGACGGCAGGATGTTGCTCAGGGTGGACTGCTCCGACCTGGGGCTCTCGGAGCTG CCTTCCAACCTCAGCGTCTTCACCTCCTACCTAGACCTCAGTATGAACAACATCAGTCAG CTGCTCCCGAATCCCCTGCCCAGTCTCCGCTTCCTGGAGGAGTTACGTCTTGCGGGAAAC GCTCTGACATACATTCCCAAGGGAGCATTCACTGGCCTTTACAGTCTTAAAGTTCTTATG CTGCAGAATAATCAGCTAAGACACGTACCCACAGAAGCTCTGCAGAATTTGCGAAGCCTT CAATCCCTGCGTCTGGATGCTAACCACATCAGCTATGTGCCCCCAAGCTGTTTCAGTGGC CTGCATTCCCTGAGGCACCTGTGGCTGGATGACAATGCGTTAACAGAAATCCCCGTCCAG GCTTTTAGAAGTTTATCGGCATTGCAAGCCATGACCTTGGCCCTGAACAAAATACACCAC ATACCAGACTATGCCTTTGGAAACCTCTCCAGCTTGGTAGTTCTACATCTCCATAACAAT AGAATCCACTCCCTGGGAAAGAAATGCTTTGATGGGCTCCACAGCCTAGAGACTTTAGAT TTAAATTACAATAACCTTGATGAATTCCCCACTGCAATTAGGACACTCTCCAACCTTAAA GAACTAGGATTTCATAGCAACAATATCAGGTCGATACCTGAGAAAGCATTTGTAGGCAAC CCTTCTCTTATTACAATACATTTCTATGACAATCCCATCCAATTTGTTGGGAGATCTGCT TTTCAACATTTACCTGAACTAAGAACACTGACTCTGAATGGTGCCTCACAAATAACTGAA TTTCCTGATTTAACTGGAACTGCAAACCTGGAGAGTCTGACTTTAACTGGAGCACAGATC TCATCTCTTCCTCAAACCGTCTGCAATCAGTTACCTAATCTCCAAGTGCTAGATCTGTCT TACAACCTATTAGAAGATTTACCCAGTTTTTCAGTCTGCCAAAAGCTTCAGAAAATTGAC CTAAGACATAATGAAATCTACGAAATTAAAGTTGACACTTTCCAGCAGTTGCTTAGCCTC CGATCGCTGAATTTGGCTTGGAACAAAATTGCTATTATTCACCCCAATGCATTTTCCACT TTGCCATCCCTAATAAAGCTGGACCTATCGTCCAACCTCCTGTCGTCTTTTCCTATAACT GGGTTACATGGTTTAACTCACTTAAAATTAACAGGAAATCATGCCTTACAGAGCTTGATA TCATCTGAAAACTTTCCAGAACTCAAGGTTATAGAAATGCCTTATGCTTACCAGTGCTGT GCATTTGGAGTGTGTGAGAATGCCTATAAGATTTCTAATCAATGGAATAAAGGTGACAAC AGCAGTATGGACGACCTTCATAAGAAAGATGCTGGAATGTTTCAGGCTCAAGATGAACGT GACCTTGAAGATTTCCTGCTTGACTTTGAGGAAGACCTGAAAGCCCTTCATTCAGTGCAG TGTTCACCTTCCCCAGGCCCCTTCAAACCCTGTGAACACCTGCTTGATGGCTGGCTGATC AGAATTGGAGTGTGGACCATAGCAGTTCTGGCACTTACTTGTAATGCTTTGGTGACTTCA ACAGTTTTCAGATCCCCTCTGTACATTTCCCCCATTAAACTGTTAATTGGGGTCATCGCA GCAGTGAACATGCTCACGGGAGTCTCCAGTGCCGTGCTGGCTGGTGTGGATGCGTTCACT TTTGGCAGCTTTGCACGACATGGTGCCTGGTGGGAGAATGGGGTTGGTTGCCATGTCATT GGTTTTTTGTCCATTTTTGCTTCAGAATCATCTGTTTTCCTGCTTACTCTGGCAGCCCTG GAGCGTGGGTTCTCTGTGAAATATTCTGCAAAATTTGAAACGAAAGCTCCATTTTCTAGC CTGAAAGTAATCATTTTGCTCTGTGCCCTGCTGGCCTTGACCATGGCCGCAGTTCCCCTG CTGGGTGGCAGCAAGTATGGCGCCTCCCCTCTCTGCCTGCCTTTGCCTTTTGGGGAGCCC AGCACCATGGGCTACATGGTCGCTCTCATCTTGCTCAATTCCCTTTGCTTCCTCATGATG ACCATTGCCTACACCAAGCTCTACTGCAATTTGGACAAGGGAGACCTGGAGAATATTTGG GACTGCTCTATGGTAAAACACATTGCCCTGTTGCTCTTCACCAACTGCATCCTAAACTGC CCTGTGGCTTTCTTGTCCTTCTCCTCTTTAATAAACCTTACATTTATCAGTCCTGAAGTA ATTAAGTTTATCCTTCTGGTGGTAGTCCCACTTCCTGCATGTCTCAATCCCCTTCTCTAC ATCTTGTTCAATCCTCACTTTAAGGAGGATCTGGTGAGCCTGAGAAAGCAAACCTACGTC TGGACAAGATCAAAACACCCAAGCTTGATGTCAATTAACTCTGATGATGTCGAAAAACAG TCCTGTGACTCAACTCAAGCCTTGGTAACCTTTACCAGCTCCAGCATCACTTATGACCTG CCTCCCAGTTCCGTGCCATCACCAGCTTATCCAGTGACTGAGAGCTGCCATCTTTCCTCT GTGGCATTTGTCCCATGTCTCTAA Clone variation with respect to NM_003667.2 >OriGene 5' read for NM_003667 unedited
NATTTGTATACGACTCATATAGGCGGCCGCGNAATTCGCACGAGGATGCTGCTCCACACC GCTCAGGCCGCGAGCAGCAGCAAGGCGCACCGCCACTGTCGCCGCTGCAGCCAGGGCTGC TCCGAAGGCCGGCGTGGCGGCAACCGGCACCTCTGTCCCCGCCGCGCTTCTCCTCGCCGC CCACGCCGTGGGGTCAGGAACGCGGCGTCTGGCGCTGCAGACGCCCGCTGAGTTGCAGAA GCCCACGGAGCGGCGCCCGGCGCGCCACGGCCCGTAGCAGTCCGGTGCTGCTCTCCGCCC GCGTCCGGCTCGTGGCCCCCTACTTCGGGCACCATGGACACCTCCCGGCTCGGTGTGCTC CTGTCCTTGCCTGTGCTGCTGCAGCTGGCGACCGGGGGCAGCTCTCCCAGGTCTGGTGTG TTGCTGAGGGGCTGCCCCACACACTGTCATTGCGAGCCCGACGGCAGGATGTTGCTCAGG GTGGACTGCTCCGACCTGGGGCTCTCGGAGCTGCCTTCCAACCTCAGCGTCTTCACCTCC TACCTAGACCTCAGTATGAACAACATCAGTCAGCTGCTCCCGAATCCCCTGCCCAGTCTC CGCTTCCTGGAGGAGTTACGTCTTGCGGGAAACGCTCTGACATACATTCCCAAGGGAGCA TTCACTGGCCTTTACAGTCTTAAAGTTCTTATGCTGCAGAATAATCAGCTAAGACACGTA CCCACAGAAGCTCTGCAGAATTTGCGAAGCCTTCAATCCTGCGTCTGGATGCTAACCCAT CAGCTATGTGCCCCAAGCTGGTTTCAGTGGCCTGCATTCCTGAGGCACCTGTGCCTGGAT GACCATGCGTTACAGAAATCCCCGCCAGCTTTTT >OriGene 3' read for NM_003667 unedited
ATGGACCGCGGCACGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTTAATAGTCACAC ATTTTATTACAAATTTATTTTTTACCCAATAGAAAAGCAAATTTGGAATCTATTTACAAG TACTATATATTTACATATATACAGTTAATGAATTTAAAGAAAATGGGCAGAGAAACACAT TATAAATCAAAGAATATGCCACTGTACAAGGTTTATTATCATTATCATGGTCCTTAATGT TACTGAACCTTTACTATAGTAATAAATACAGTTCTATATTTACACATCTTATAAAACATC TCATAAATGTATTTTTTCAAATCCAAGTTAAAACATCTGATCAAAATAAACATGCTTATA TAAAAATAAATCTACCTAACAGCCATTTGGTTTGGATGTATTGAAGCTAATATAGGATAA TAGAAGGTAAGAATTAATACTTTGACTTTTCTTATTTAATAACTTGCTTCTTAAAATACC TAACACAGTATTAATATGGAATAAGCAGAGAAGTAATGTTCCTAACATCAAGTGGGTTAT CCAGAGAGAACACAGCTAAAACCAAGCTAAATAAACAGGATAATACGTTACTGAGTCTCT TGAGTCCAAAGTGGTGTCAGATATTGGGTTTGCCAGAGCTACTAGAGATACATGTGTAAG AAGTTGTATCAGTGGACTTAATTTATGTAATGTGCACATTTGATCATTAAGATGCACATC AGTTTGAATCAACTGATAAAACTTATTGCAAAAATTCTTTACTAACCCAGAAAAAAATCC CAGATTGCTTACTTTNNCTTTTCAGGTATGTCATTGCTGGCAGTAGGAATTCCCTTCTGA GCTTTGCNGCACCAGGAGTTAAAANNNCAATCAGATAGACATACGTCACCTGTGCATGAA TGCCTTANNNTACATTAAGAATTTTGGCCAGTTTTTCTCCAAATACTTGNAGCAGTTTTA CCCATGAGGGTGGAACCATCTAGGGCTGAACATAAACCTGGTATCCCTAAAATGACN |
| Restriction Sites | NotI-NotI |
| ACCN | NM_003667 |
| Insert Size | 2724 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_003667.2, NP_003658.1 |
| RefSeq Size | 2880 bp |
| RefSeq ORF | 2724 bp |
| Locus ID | 8549 |
| UniProt ID | O75473 |
| Domains | 7tm_1, LRRNT, LRR, LRR_TYP, LRR_PS |
| Protein Families | Druggable Genome, GPCR, Transmembrane |
| Gene Summary | The protein encoded by this gene is a leucine-rich repeat-containing receptor (LGR) and member of the G protein-coupled, 7-transmembrane receptor (GPCR) superfamily. The encoded protein is a receptor for R-spondins and is involved in the canonical Wnt signaling pathway. This protein plays a role in the formation and maintenance of adult intestinal stem cells during postembryonic development. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2015] Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Citations (3)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
CBB1003, a lysine-specific demethylase 1 inhibitor, suppresses colorectal cancer cells growth through down-regulation of leucine-rich repeat-containing G-protein-coupled receptor 5 expression
,Hsu, HC;Liu, YS;Tseng, KC;Yang, TS;Yeh, CY;You, JF;Hung, HY;Chen, SJ;Chen, HC;,
J. Cancer Res. Clin. Oncol. July 2014
,PubMed ID 25060070
[LGR5]
|
|
Lgr5 promotes cancer stemness and confers chemoresistance through ABCB1 in colorectal cancer.
,null,
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie
,PubMed ID 24138824
[LGR5]
|
|
Overexpression of Lgr5 correlates with resistance to 5-FU-based chemotherapy in colorectal cancer.
,null,
International journal of colorectal disease
,PubMed ID 23784051
[LGR5]
|
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC212825 | LGR5 (Myc-DDK-tagged)-Human leucine-rich repeat containing G protein-coupled receptor 5 (LGR5) |
CNY 9,960.00 |
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| RC212825L1 | Lenti ORF clone of Human leucine-rich repeat containing G protein-coupled receptor 5 (LGR5), Myc-DDK-tagged |
CNY 12,360.00 |
|
| RC212825L2 | Lenti ORF clone of Human leucine-rich repeat containing G protein-coupled receptor 5 (LGR5), mGFP tagged |
CNY 5,890.00 |
|
| RC212825L3 | Lenti ORF clone of Human leucine-rich repeat containing G protein-coupled receptor 5 (LGR5), Myc-DDK-tagged |
CNY 12,360.00 |
|
| RC212825L4 | Lenti ORF clone of Human leucine-rich repeat containing G protein-coupled receptor 5 (LGR5), mGFP tagged |
CNY 12,360.00 |
|
| RG212825 | LGR5 (tGFP-tagged) - Human leucine-rich repeat containing G protein-coupled receptor 5 (LGR5) |
CNY 11,560.00 |
