RANBP9 (NM_005493) Human Tagged ORF Clone

CAT#: RC212902

RANBP9 (Myc-DDK-tagged)-Human RAN binding protein 9 (RANBP9)

ORF Plasmid: DDK tGFP



  "NM_005493" in other vectors (6)

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CNY 5,344.00

CNY 7,700.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


Rabbit monoclonal antibody against RanBP9 (C-term) (EP1353Y )
    • 100 ul

CNY 6,336.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms BPM-L; BPM90; RanBP7; RANBPM
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC212902 representing NM_005493
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCCGGGCAGCCGCCGCCGCCGCCGCCGCAGCAGCAGCAACAGCAGCAGCAGCTGTCGCCGCCACCGC
CGGCGGCCTTGGCCCCAGTCTCCGGAGTCGTCCTGCCGGCGCCCCCGGCCGTCAGCGCCGGCTCTTCTCC
GGCCGGCTCGCCCGGCGGCGGTGCGGGCGGCGAAGGCTTAGGGGCCGCGGCGGCCGCCCTGCTCCTCCAC
CCTCCGCCGCCGCCGCCCCCGGCCACCGCGGCCCCGCCGCCCCCGCCGCCGCCCCCGCCTCCCCCTGCCT
CAGCGGCTGCCCCCGCCAGCGGGCCGCCCGCTCCCCCGGGCCTTGCAGCGGGCCCCGGCCCGGCTGGAGG
AGCCCCGACCCCAGCTCTGGTGGCGGGCAGCAGCGCCGCGGCCCCCTTCCCTCACGGGGACTCGGCCCTG
AACGAGCAGGAGAAGGAGTTGCAGCGGCGGCTGAAGCGTCTCTACCCGGCCGTGGACGAACAAGAGACGC
CGCTGCCTCGGTCCTGGAGCCCGAAGGACAAGTTCAGCTACATCGGCCTCTCTCAGAACAACCTGCGGGT
GCACTACAAAGGTCATGGCAAAACCCCAAAAGATGCCGCGTCAGTTCGAGCCACGCATCCAATACCAGCA
GCCTGTGGGATTTATTATTTTGAAGTAAAAATTGTCAGTAAGGGAAGAGATGGTTACATGGGAATTGGTC
TTTCTGCTCAAGGTGTGAACATGAATAGACTACCAGGTTGGGATAAGCATTCATATGGTTACCATGGGGA
TGATGGACATTCGTTTTGTTCTTCTGGAACTGGACAACCTTATGGACCAACTTTCACTACTGGTGATGTC
ATTGGCTGTTGTGTTAATCTTATCAACAATACCTGCTTTTACACCAAGAATGGACATAGTTTAGGTATTG
CTTTCACTGACCTACCGCCAAATTTGTATCCTACTGTGGGGCTTCAAACACCAGGAGAAGTGGTCGATGC
CAATTTTGGGCAACATCCTTTCGTGTTTGATATAGAAGACTATATGCGGGAGTGGAGAACCAAAATCCAG
GCACAGATAGATCGATTTCCTATCGGAGATCGAGAAGGAGAATGGCAGACCATGATACAAAAAATGGTTT
CATCTTATTTAGTCCACCATGGGTACTGTGCCACAGCAGAGGCCTTTGCCAGATCTACAGACCAGACCGT
TCTAGAAGAATTAGCTTCCATTAAGAATAGACAAAGAATTCAGAAATTGGTATTAGCAGGAAGAATGGGA
GAAGCCATTGAAACAACACAACAGTTATACCCAAGTTTACTTGAAAGAAATCCTAATCTCCTTTTCACAT
TAAAAGTGCGTCAGTTTATAGAAATGGTGAATGGTACAGATAGTGAAGTACGATGTTTGGGAGGCCGAAG
TCCAAAGTCTCAAGACAGTTATCCTGTTAGTCCTCGACCTTTTAGTAGTCCAAGTATGAGCCCCAGCCAT
GGAATGAATATCCACAATTTAGCATCAGGCAAAGGAAGCACCGCACATTTTTCAGGTTTTGAAAGTTGTA
GTAATGGTGTAATATCAAATAAAGCACATCAATCATATTGCCATAGTAATAAACACCAGTCATCCAACTT
GAATGTACCAGAACTAAACAGTATAAATATGTCAAGATCACAGCAAGTTAATAACTTCACCAGTAATGAT
GTAGACATGGAAACAGATCACTACTCCAATGGAGTTGGAGAAACTTCATCCAATGGTTTCCTAAATGGTA
GCTCTAAACATGACCACGAAATGGAAGATTGTGACACCGAAATGGAAGTTGATTCAAGTCAGTTGAGACG
CCAGTTGTGTGGAGGAAGTCAGGCCGCCATAGAAAGAATGATCCACTTTGGACGAGAGCTGCAAGCAATG
AGTGAACAGCTAAGGAGAGACTGTGGCAAGAACACTGCAAACAAAAAAATGTTGAAGGATGCATTCAGTC
TACTAGCATATTCAGATCCCTGGAACAGCCCAGTTGGAAATCAGCTTGACCCGATTCAGAGAGAACCTGT
GTGCTCAGCTCTTAACAGTGCAATATTAGAAACCCACAATCTGCCAAAGCAACCTCCACTTGCCCTAGCA
ATGGGACAGGCCACACAATGTCTAGGACTGATGGCTCGATCAGGAATTGGATCCTGCGCATTTGCCACAG
TGGAAGACTACCTACAT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC212902 representing NM_005493
Red=Cloning site Green=Tags(s)

MSGQPPPPPPQQQQQQQQLSPPPPAALAPVSGVVLPAPPAVSAGSSPAGSPGGGAGGEGLGAAAAALLLH
PPPPPPPATAAPPPPPPPPPPPASAAAPASGPPAPPGLAAGPGPAGGAPTPALVAGSSAAAPFPHGDSAL
NEQEKELQRRLKRLYPAVDEQETPLPRSWSPKDKFSYIGLSQNNLRVHYKGHGKTPKDAASVRATHPIPA
ACGIYYFEVKIVSKGRDGYMGIGLSAQGVNMNRLPGWDKHSYGYHGDDGHSFCSSGTGQPYGPTFTTGDV
IGCCVNLINNTCFYTKNGHSLGIAFTDLPPNLYPTVGLQTPGEVVDANFGQHPFVFDIEDYMREWRTKIQ
AQIDRFPIGDREGEWQTMIQKMVSSYLVHHGYCATAEAFARSTDQTVLEELASIKNRQRIQKLVLAGRMG
EAIETTQQLYPSLLERNPNLLFTLKVRQFIEMVNGTDSEVRCLGGRSPKSQDSYPVSPRPFSSPSMSPSH
GMNIHNLASGKGSTAHFSGFESCSNGVISNKAHQSYCHSNKHQSSNLNVPELNSINMSRSQQVNNFTSND
VDMETDHYSNGVGETSSNGFLNGSSKHDHEMEDCDTEMEVDSSQLRRQLCGGSQAAIERMIHFGRELQAM
SEQLRRDCGKNTANKKMLKDAFSLLAYSDPWNSPVGNQLDPIQREPVCSALNSAILETHNLPKQPPLALA
MGQATQCLGLMARSGIGSCAFATVEDYLH

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_005493
ORF Size 2187 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_005493.3
RefSeq Size 3132 bp
RefSeq ORF 2190 bp
Locus ID 10048
UniProt ID Q96S59
Domains SPRY, LisH, CTLH
Protein Families Druggable Genome
MW 77.7 kDa
Gene Summary This gene encodes a protein that binds RAN, a small GTP binding protein belonging to the RAS superfamily that is essential for the translocation of RNA and proteins through the nuclear pore complex. The protein encoded by this gene has also been shown to interact with several other proteins, including met proto-oncogene, homeodomain interacting protein kinase 2, androgen receptor, and cyclin-dependent kinase 11. [provided by RefSeq, Jul 2008]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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