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ALOXE3 (NM_021628) Human Tagged ORF Clone
CAT＃: RC212945
ALOXE3 (Myc-DDK-tagged)-Human arachidonate lipoxygenase 3 (ALOXE3), transcript variant 2
ORF Plasmid: tGFP
"NM_021628" in other vectors (6)
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CNY 7,808.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | ARCI3; E-LOX; eLOX-3; eLOX3 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC212945 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAGTGTACCGCCTGTGTGTGACCACTGGTCCCTACCTGAGGGCCGGCACACTGGACAACATCTCTG TCACACTGGTGGGCACGTGTGGTGAAAGCCCCAAGCAGCGGCTAGATCGAATGGGCAGGGACTTCGCCCC TGGATCGGTACAGAAGTACAAGGTGCGTTGCACAGCGGAGCTGGGTGAGCTCTTGCTGCTGCGTGTACAC AAGGAGCGCTACGCTTTCTTCCGCAAGGACTCTTGGTACTGTAGCCGCATCTGTGTCACCGAACCGGATG GTAGTGTATCCCACTTCCCCTGCTATCAGTGGATTGAAGGCTACTGCACCGTGGAGCTGAGGCCAGGAAC AGCAAGAACTATTTGTCAGGACTCTCTTCCCCTCCTCCTGGATCACAGGACACGGGAGCTCCGGGCCCGA CAAGAATGCTACCGCTGGAAGATCTATGCCCCTGGCTTCCCCTGCATGGTAGACGTCAACAGCTTTCAGG AGATGGAGTCAGACAAGAAATTTGCCTTGACAAAGACGACAACTTGTGTAGACCAGGGTGACAGCAGTGG GAATCGGTACCTGCCCGGCTTCCCCATGAAAATTGACATCCCATCCCTGATGTACATGGAGCCCAATGTT CGATACTCAGCCACCAAGACGATCTCGCTGCTCTTCAATGCCATCCCTGCGTCCTTGGGAATGAAGCTTC GAGGGCTGTTGGATCGCAAGGGCTCCTGGAAGAAGCTGGATGACATGCAGAACATCTTCTGGTGCCATAA GACCTTCACGACAAAGTATGTCACAGAGCACTGGTGTGAAGATCACTTCTTTGGGTACCAGTACCTGAAT GGTGTCAATCCCGTCATGCTCCACTGCATCTCTAGCTTGCCCAGCAAGCTGCCTGTCACCAATGACATGG TGGCCCCCTTGCTGGGACAGGACACATGCCTGCAGACAGAGCTAGAGAGGGGGAACATCTTCCTAGCGGA CTACTGGATCCTGGCGGAGGCCCCCACCCACTGCCTAAACGGCCGCCAGCAGTACGTGGCCGCCCCACTG TGCCTGCTGTGGCTCAGCCCCCAGGGGGCGCTGGTGCCCTTGGCCATCCAGCTCAGCCAGACCCCCGGGC CTGACAGCCCCATCTTCCTGCCCACTGACTCCGAATGGGACTGGCTGCTGGCCAAGACGTGGGTGCGCAA CTCTGAGTTCCTGGTGCACGAAAACAACACGCACTTTCTGTGCACGCATTTGCTGTGCGAGGCCTTCGCC ATGGCCACGCTGCGCCAGCTGCCGCTCTGCCACCCCATCTACAAGCTCCTACTCCCCCACACTCGATACA CGCTGCAGGTGAACACCATCGCGAGGGCCACGCTGCTCAACCCCGAGGGCCTCGTGGACCAGGTCACGTC CATCGGGAGGCAAGGCCTCATCTACCTCATGAGCACGGGCCTGGCCCACTTCACCTACACCAATTTCTGC CTTCCGGACAGCCTGCGGGCCCGCGGCGTCCTGGCTATCCCCAACTACCACTACCGAGACGACGGCCTGA AGATCTGGGCGGCCATTGAGAGCTTTGTCTCAGAAATCGTGGGCTACTATTATCCCAGTGACGCATCTGT GCAGCAGGATTCGGAGCTGCAGGCCTGGACTGGCGAGATTTTTGCTCAGGCGTTCCTGGGCCGGGAAAGC TCAGGTTTCCCAAGCCGGCTGTGCACCCCAGGAGAGATGGTGAAGTTCCTCACTGCAATCATCTTCAATT GCTCTGCCCAGCACGCTGCTGTCAACAGTGGGCAGCATGACTTTGGGGCCTGGATGCCCAATGCTCCATC ATCCATGAGGCAGCCCCCACCCCAGACCAAGGGGACCACCACCCTGAAGACTTACCTAGACACCCTCCCT GAAGTGAACATCAGCTGTAACAACCTCCTCCTCTTCTGGTTGGTTAGCCAAGAACCCAAGGACCAGAGGC CCCTGGGCACCTACCCAGATGAGCACTTCACAGAGGAGGCCCCGAGGCGGAGCATCGCCGCCTTCCAGAG CCGCCTGGCCCAGATCTCAAGGGACATCCAGGAGCGGAACCAGGGTCTGGCACTGCCCTACACCTACCTG GACCCTCCCCTCATTGAGAACAGCGTCTCCATC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC212945 protein sequence
Red=Cloning site Green=Tags(s) MAVYRLCVTTGPYLRAGTLDNISVTLVGTCGESPKQRLDRMGRDFAPGSVQKYKVRCTAELGELLLLRVH KERYAFFRKDSWYCSRICVTEPDGSVSHFPCYQWIEGYCTVELRPGTARTICQDSLPLLLDHRTRELRAR QECYRWKIYAPGFPCMVDVNSFQEMESDKKFALTKTTTCVDQGDSSGNRYLPGFPMKIDIPSLMYMEPNV RYSATKTISLLFNAIPASLGMKLRGLLDRKGSWKKLDDMQNIFWCHKTFTTKYVTEHWCEDHFFGYQYLN GVNPVMLHCISSLPSKLPVTNDMVAPLLGQDTCLQTELERGNIFLADYWILAEAPTHCLNGRQQYVAAPL CLLWLSPQGALVPLAIQLSQTPGPDSPIFLPTDSEWDWLLAKTWVRNSEFLVHENNTHFLCTHLLCEAFA MATLRQLPLCHPIYKLLLPHTRYTLQVNTIARATLLNPEGLVDQVTSIGRQGLIYLMSTGLAHFTYTNFC LPDSLRARGVLAIPNYHYRDDGLKIWAAIESFVSEIVGYYYPSDASVQQDSELQAWTGEIFAQAFLGRES SGFPSRLCTPGEMVKFLTAIIFNCSAQHAAVNSGQHDFGAWMPNAPSSMRQPPPQTKGTTTLKTYLDTLP EVNISCNNLLLFWLVSQEPKDQRPLGTYPDEHFTEEAPRRSIAAFQSRLAQISRDIQERNQGLALPYTYL DPPLIENSVSI TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_021628 |
| ORF Size | 2133 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_021628.3 |
| RefSeq Size | 3251 bp |
| RefSeq ORF | 2136 bp |
| Locus ID | 59344 |
| UniProt ID | Q9BYJ1 |
| Protein Families | Druggable Genome |
| MW | 80.5 kDa |
| Gene Summary | This gene is a member of the lipoxygenase family, which are catabolized by arachidonic acid-derived compounds. The encoded enzyme is a hydroperoxide isomerase that synthesizes a unique type of epoxy alcohol (8R-hydroxy-11R,12R-epoxyeicosa-5Z,9E,14Z-trienoic acid) from 12R-hydroperoxyeicosatetraenoic acid (12R-HPETE). This epoxy alcohol can activate the the nuclear receptor peroxisome proliferator-activated receptor alpha (PPARalpha), which is implicated in epidermal differentiation. Loss of function of the enzyme encoded by this gene results in ichthyosis, implicating the function of this gene in the differentiation of human skin. This gene is part of a cluster of lipoxygenase genes on 17p13.1. Mutations in this gene result in nonbullous congenital ichthyosiform erythroderma (NCIE). Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Sep 2009] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC212945L1 | Lenti ORF clone of Human arachidonate lipoxygenase 3 (ALOXE3), transcript variant 2, Myc-DDK-tagged |
CNY 10,208.00 |
|
| RC212945L2 | Lenti ORF clone of Human arachidonate lipoxygenase 3 (ALOXE3), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
| RC212945L3 | Lenti ORF clone of Human arachidonate lipoxygenase 3 (ALOXE3), transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC212945L4 | Lenti ORF clone of Human arachidonate lipoxygenase 3 (ALOXE3), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
| RG212945 | ALOXE3 (tGFP-tagged) - Human arachidonate lipoxygenase 3 (ALOXE3), transcript variant 2 |
CNY 9,408.00 |
|
| SC304947 | ALOXE3 (untagged)-Human arachidonate lipoxygenase 3 (ALOXE3), transcript variant 2 |
CNY 7,824.00 |
