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KCNH4 (NM_012285) Human Tagged ORF Clone
CAT＃: RC212999
KCNH4 (Myc-DDK-tagged)-Human potassium voltage-gated channel, subfamily H (eag-related), member 4 (KCNH4)
ORF Plasmid: tGFP
"NM_012285" in other vectors (4)
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CNY 10,624.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | BEC2; ELK1; Kv12.3 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC212999 representing NM_012285
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCGGTCATGAAGGGGTTGCTGGCCCCGCAAAACACCTTCCTGGACACCATCGCCACCCGTTTTGACG GAACGCACAGCAACTTCCTGCTGGCCAACGCACAGGGCACACGGGGCTTTCCCATCGTCTACTGCTCCGA CGGCTTCTGCGAGCTCACAGGCTACGGTCGCACCGAGGTCATGCAGAAGACCTGCAGCTGCCGTTTCCTC TACGGCCCAGAGACCAGTGAGCCAGCCCTGCAGCGTCTGCACAAAGCCCTGGAGGGCCACCAGGAGCACC GGGCTGAAATCTGCTTCTACCGCAAGGATGGCTCAGCCTTTTGGTGCCTCCTGGACATGATGCCCATCAA GAATGAGATGGGGGAGGTCGTGCTGTTCCTCTTTTCCTTCAAGGATATCACTCAGAGTGGAAGCCCAGGA CTTGGCCCCCAAGGAGGCCGCGGGGACAGTAATCACGAAAACTCCCTTGGTAGAAGGGGAGCCACCTGGA AATTTCGGTCTGCCAGAAGACGGAGCCGTACTGTCCTACACCGACTGACCGGCCACTTTGGCCGCCGGGG CCAGGGAGGCATGAAGGCCAATAATAACGTGTTTGAGCCAAAGCCATCAGTGCCCGAGTACAAGGTGGCC TCCGTGGGGGGGTCTCGCTGCCTCCTCCTCCACTACAGCGTCTCCAAGGCCATCTGGGACGGCCTTATCC TCCTTGCCACCTTCTACGTTGCGGTCACCGTCCCCTACAATGTCTGTTTCTCGGGTGACGATGACACCCC CATCACTTCGCGACACACCCTTGTCAGCGACATCGCCGTGGAAATGCTCTTCATCCTAGATATCATCCTG AACTTCCGCACCACCTATGTGTCCCAGTCCGGCCAGGTAATCTCTGCTCCTCGTTCCATTGGCCTCCACT ACCTGGCCACCTGGTTCTTCATCGACCTTATTGCTGCTCTGCCCTTTGACCTGCTTTACATCTTCAACAT CACCGTGACCTCGCTGGTGCACCTACTGAAGACAGTGCGGCTGTTGCGGCTGCTGCGGCTGCTGCAGAAG CTGGAGCGGTACTCTCAGTGCAGTGCTGTGGTGCTCACGCTGCTCATGTCGGTCTTTGCGCTCCTTGCCC ACTGGATGGCCTGCATCTGGTATGTCATCGGGCGCCGGGAGATGGAGGCCAATGACCCGCTGCTCTGGGA CATTGGCTGGTTGCATGAGTTGGGCAAGCGTCTGGAGGTGCCCTATGTCAATGGCTCGGTGGGCGGCCCA TCACGGCGCAGCGCCTACATCGCGGCACTGTACTTCACTCTAAGCAGCCTCACCAGTGTGGGCTTTGGCA ACGTGTGTGCCAACACCGACGCGGAGAAGATCTTCTCCATCTGCACGATGCTCATAGGCGCCCTGATGCA CGCTGTGGTGTTCGGGAACGTGACAGCCATCATCCAGCGCATGTACTCGCGCCGCTCGCTCTACCACAGC CGCATGAAGGACCTCAAGGACTTCATCCGTGTGCACCGCCTGCCGCGGCCGCTCAAGCAGCGCATGCTCG AATACTTCCAGACCACGTGGGCCGTCAACAGCGGCATCGACGCCAACGAGTTACTGCGTGACTTCCCAGA CGAGCTGAGAGCTGACATTGCTATGCACCTGAATCGGGAGATCCTGCAGCTGCCGTTGTTCGGGGCAGCG AGCAGGGGCTGCCTGCGGGCCCTATCGCTGCACATCAAGACCTCGTTCTGCGCTCCGGGCGAGTACCTGT TGCGCCGTGGGGATGCCCTGCAGGCACATTACTATGTCTGCTCCGGCTCGCTTGAGGTGCTCCGAGACAA CATGGTGCTGGCCATCCTGGGGAAGGGGGACCTGATTGGAGCAGATATCCCTGAGCCGGGGCAGGAGCCT GGGTTGGGAGCAGACCCAAACTTCGTGCTAAAGACCAGTGCTGATGTGAAAGCTCTGACCTACTGTGGCC TGCAGCAGCTGAGCAGCCGAGGGCTGGCTGAGGTCCTGAGGCTCTATCCTGAGTATGGGGCTGCCTTCCG GGCTGGCCTGCCCCGGGACCTCACCTTCAACCTGCGCCAGGGCTCTGACACCAGTGGCCTCAGCCGCTTT TCCCGATCCCCTCGCCTCTCCCAGCCCCGCTCAGAAAGCCTCGGCTCCTCCTCAGACAAGACGCTGCCAT CCATCACAGAGGCCGAGAGTGGCGCGGAGCCTGGGGGTGGTCCCAGGCCCCGACGGCCCCTCCTGCTGCC CAACCTCAGCCCAGCACGGCCTCGGGGCTCCCTGGTCAGCCTTTTGGGCGAGGAGCTGCCCCCATTCTCA GCCCTTGTCTCCTCTCCTTCCTTATCCCCATCCCTGTCCCCTGCCCTGGCTGGCCAGGGCCACAGTGCCT CCCCTCACGGCCCCCCCAGGTGCTCTGCTGCCTGGAAGCCCCCTCAGCTTCTCATTCCCCCACTGGGAAC CTTTGGACCTCCGGACCTCAGTCCCCGGATAGTGGATGGCATTGAGGACTCTGGCAGCACAGCTGAGGCC CCTTCATTCCGATTCAGCAGGAGGCCTGAACTGCCAAGGCCCCGCTCCCAGGCGCCCCCTACAGGGACCA GGCCCAGCCCAGAATTGGCCAGTGAGGCTGAGGAGGTGAAGGAAAAGGTTTGCCGGCTGAACCAGGAGAT CTCTCGTCTCAATCAGGAGGTGTCTCAGCTTAGCCGGGAGCTGCGGCACATCATGGGCCTGCTGCAGGCC AGGCTGGGTCCCCCAGGCCACCCAGCAGGCTCCGCTTGGACCCCAGACCCTCCTTGTCCACAGCTGAGGC CACCATGCCTCTCTCCTTGTGCGTCCAGACCACCACCCAGCCTCCAGGATACTACGCTTGCTGAAGTTCA CTGCCCAGCCAGTGTGGGGACCATGGAGACAGGGACTGCGCTCCTGGACTTGAGACCTTCCATATTGCCC CCCTACCCCTCAGAGCCTGACCCTCTGGGACCCTCTCCAGTGCCAGAGGCCTCACCCCCAACCCCAAGCC TCTTGAGGCACAGTTTCCAGTCCAGGTCAGACACGTTCCAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC212999 representing NM_012285
Red=Cloning site Green=Tags(s) MPVMKGLLAPQNTFLDTIATRFDGTHSNFLLANAQGTRGFPIVYCSDGFCELTGYGRTEVMQKTCSCRFL YGPETSEPALQRLHKALEGHQEHRAEICFYRKDGSAFWCLLDMMPIKNEMGEVVLFLFSFKDITQSGSPG LGPQGGRGDSNHENSLGRRGATWKFRSARRRSRTVLHRLTGHFGRRGQGGMKANNNVFEPKPSVPEYKVA SVGGSRCLLLHYSVSKAIWDGLILLATFYVAVTVPYNVCFSGDDDTPITSRHTLVSDIAVEMLFILDIIL NFRTTYVSQSGQVISAPRSIGLHYLATWFFIDLIAALPFDLLYIFNITVTSLVHLLKTVRLLRLLRLLQK LERYSQCSAVVLTLLMSVFALLAHWMACIWYVIGRREMEANDPLLWDIGWLHELGKRLEVPYVNGSVGGP SRRSAYIAALYFTLSSLTSVGFGNVCANTDAEKIFSICTMLIGALMHAVVFGNVTAIIQRMYSRRSLYHS RMKDLKDFIRVHRLPRPLKQRMLEYFQTTWAVNSGIDANELLRDFPDELRADIAMHLNREILQLPLFGAA SRGCLRALSLHIKTSFCAPGEYLLRRGDALQAHYYVCSGSLEVLRDNMVLAILGKGDLIGADIPEPGQEP GLGADPNFVLKTSADVKALTYCGLQQLSSRGLAEVLRLYPEYGAAFRAGLPRDLTFNLRQGSDTSGLSRF SRSPRLSQPRSESLGSSSDKTLPSITEAESGAEPGGGPRPRRPLLLPNLSPARPRGSLVSLLGEELPPFS ALVSSPSLSPSLSPALAGQGHSASPHGPPRCSAAWKPPQLLIPPLGTFGPPDLSPRIVDGIEDSGSTAEA PSFRFSRRPELPRPRSQAPPTGTRPSPELASEAEEVKEKVCRLNQEISRLNQEVSQLSRELRHIMGLLQA RLGPPGHPAGSAWTPDPPCPQLRPPCLSPCASRPPPSLQDTTLAEVHCPASVGTMETGTALLDLRPSILP PYPSEPDPLGPSPVPEASPPTPSLLRHSFQSRSDTFH TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_012285 |
| ORF Size | 3051 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_012285.3 |
| RefSeq Size | 3920 bp |
| RefSeq ORF | 3054 bp |
| Locus ID | 23415 |
| UniProt ID | Q9UQ05 |
| Protein Families | Druggable Genome, Ion Channels: Potassium, Transmembrane |
| MW | 111.5 kDa |
| Gene Summary | Voltage-gated potassium (Kv) channels represent the most complex class of voltage-gated ion channels from both functional and structural standpoints. Their diverse functions include regulating neurotransmitter release, heart rate, insulin secretion, neuronal excitability, epithelial electrolyte transport, smooth muscle contraction, and cell volume. This gene encodes a member of the potassium channel, voltage-gated, subfamily H. This member is a pore-forming (alpha) subunit. The gene is brain-specific, and located in the neocortex and the striatum. It may be involved in cellular excitability of restricted neurons in the central nervous system. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC212999L3 | Lenti ORF clone of Human potassium voltage-gated channel, subfamily H (eag-related), member 4 (KCNH4), Myc-DDK-tagged |
CNY 12,070.00 |
|
| RC212999L4 | Lenti ORF clone of Human potassium voltage-gated channel, subfamily H (eag-related), member 4 (KCNH4), mGFP tagged |
CNY 12,070.00 |
|
| RG212999 | KCNH4 (tGFP-tagged) - Human potassium voltage-gated channel, subfamily H (eag-related), member 4 (KCNH4) |
CNY 11,210.00 |
|
| SC310004 | KCNH4 (untagged)-Human potassium voltage-gated channel, subfamily H (eag-related), member 4 (KCNH4) |
CNY 10,632.00 |
