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USP20 (NM_001008563) Human Tagged ORF Clone
CAT＃: RC213090
USP20 (Myc-DDK-tagged)-Human ubiquitin specific peptidase 20 (USP20), transcript variant 2
ORF Plasmid: tGFP
"NM_001008563" in other vectors (5)
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CNY 6,688.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | hVDU2; LSFR3A; VDU2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC213090 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGGACTCCAGGGACCTTTGCCCTCACCTTGACTCCATAGGAGAGGTGACCAAAGAGGACTTGCTGC TCAAATCTAAGGGAACCTGTCAGTCGTGTGGGGTCACCGGACCAAACCTATGGGCCTGTCTGCAGGTTGC CTGCCCCTATGTTGGCTGCGGAGAATCCTTCGCTGACCACAGCACCATTCATGCACAGGCAAAAAAGCAC AACTTGACCGTGAACCTGACCACGTTCCGACTGTGGTGTTACGCCTGTGAGAAGGAGGTATTCCTGGAGC AGCGGCTGGCAGCCCCTCTGCTGGGCTCCTCTTCCAAGTTCTCTGAACAGGACTCCCCGCCACCCTCCCA CCCTCTGAAAGCTGTTCCTATTGCTGTGGCTGATGAAGGAGAGTCTGAGTCAGAGGATGATGACCTGAAA CCTCGAGGCCTCACGGGCATGAAGAACCTCGGGAACTCCTGCTACATGAACGCCGCCCTGCAGGCCCTGT CCAATTGCCCGCCGCTGACTCAGTTCTTCTTGGAGTGTGGCGGCCTGGTGCGCACAGATAAGAAGCCAGC CCTGTGCAAGAGCTACCAGAAGCTGGTCTCTGAGGTCTGGCATAAGAAACGGCCAAGCTACGTGGTCCCC ACCAGTCTGTCTCATGGGATCAAGTTGGTCAACCCAATGTTCCGAGGCTATGCCCAGCAGGACACCCAAG AGTTCCTTCGCTGCCTGATGGACCAGCTGCACGAGGAGCTCAAGGAGCCGGTGGTGGCCACGGTGGCGCT GACGGAGGCTCGGGACTCAGATTCGAGTGACACGGATGAGAAACGGGAGGGTGACCGGAGCCCATCAGAA GATGAGTTCTTGTCCTGTGACTCGAGCAGTGACCGGGGTGAGGGTGACGGGCAGGGGCGTGGCGGGGGCA GCTCGCAGGCCGAGACGGAGCTGCTGATCCCAGATGAGGCGGGCCGAGCCATCTCTGAGAAGGAGCGGAT GAAGGACCGCAAGTTCTCCTGGGGCCAGCAGCGTACAAACTCGGAGCAAGTGGACGAGGACGCTGATGTG GACACTGCCATGGCTGCCCTTGACCAGCCCGCGGAGGCCCAGCCCCCGTCACCACGGTCCTCCAGCCCCT GCCGGACGCCAGAGCCGGACAATGATGCTCACCTACGCAGCTCCTCTCGCCCCTGCAGCCCCGTCCACCA CCACGAGGGCCATGCCAAGCTGTCTAGCAGCCCCCCTCGTGCAAGCCCCGTGAGGATGGCACCGTCGTAC GTGCTCAAGAAAGCCCAGGTATTGAGTGCTGGCAGCCGGAGGCGGAAGGAGCAGCGCTACCGCAGCGTCA TCTCAGACATCTTTGACGGCTCCATTCTCAGCCTCGTGCAGTGTCTCACCTGTGACCGGGTATCCACCAC AGTGGAAACGTTCCAGGACTTATCACTGCCCATTCCTGGAAAGGAGGACCTGGCCAAGCTCCATTCAGCC ATCTACCAGAATGTGCCGGCCAAGCCAGGCGCCTGTGGGGACAGCTATGCCGCCCAGGGCTGGCTGGCCT TCATTGTGGAGTACATCCGACGGTTTGTGGTATCCTGTACCCCCAGCTGGTTTTGGGGGCCTGTCGTCAC CCTGGAAGACTGCCTTGCTGCCTTCTTTGCCGCTGATGAGTTAAAGGGTGACAACATGTACAGCTGTGAG CGGTGTAAGAAGCTGCGGAACGGAGTGAAGTACTGCAAAGTCCTGCGGTTGCCCGAGATCCTGTGCATTC ACCTAAAGCGCTTTCGGCACGAGGTGATGTACTCATTCAAGATCAACAGCCACGTCTCCTTCCCCCTCGA GGGGCTCGACCTGCGCCCCTTCCTTGCCAAGGAGTGCACATCCCAGATCACCACCTACGACCTCCTCTCG GTCATCTGCCACCACGGCACGGCAGGCAGTGGGCACTACATCGCCTACTGCCAGAACGTGATCAATGGGC AGTGGTACGAGTTTGATGACCAGTACGTCACAGAAGTCCACGAGACGGTGGTGCAGAACGCCGAGGGCTA CGTACTCTTCTACAGGAAGAGCAGCGAGGAGGCCATGCGGGAGCGACAGCAGGTGGTGTCCCTGGCCGCC ATGCGGGAGCCCAGCCTGCTGCGGTTCTACGTGTCCCGCGAGTGGCTCAACAAGTTCAACACCTTCGCGG AGCCAGGCCCCATCACCAACCAGACCTTCCTCTGCTCCCACGGAGGCATCCCGCCCCACAAATACCACTA CATCGACGACCTGGTGGTCATCCTGCCCCAGAACGTCTGGGAGCACCTGTACAACAGATTCGGGGGTGGC CCCGCCGTGAACCACCTGTACGTGTGCTCCATCTGCCAGGTGGAGATCGAGGCACTGGCCAAGCGCAGGA GGATCGAGATCGACACCTTCATCAAGTTGAACAAGGCCTTCCAGGCCGAGGAGTCGCCGGGCGTCATCTA CTGCATCAGCATGCAGTGGTTCCGGGAGTGGGAGGCGTTCGTCAAGGGGAAGGACAACGAGCCCCCCGGG CCCATTGACAACAGCAGGATTGCACAGGTCAAAGGAAGCGGCCATGTCCAGCTGAAGCAGGGAGCTGACT ACGGGCAGATTTCGGAGGAGACCTGGACCTACCTGAACAGCCTGTATGGAGGTGGCCCCGAGATTGCCAT CCGCCAGAGTGTGGCGCAGCCGCTGGGCCCAGAGAACCTGCACGGGGAGCAGAAGATCGAAGCCGAGACG CGGGCCGTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC213090 protein sequence
Red=Cloning site Green=Tags(s) MGDSRDLCPHLDSIGEVTKEDLLLKSKGTCQSCGVTGPNLWACLQVACPYVGCGESFADHSTIHAQAKKH NLTVNLTTFRLWCYACEKEVFLEQRLAAPLLGSSSKFSEQDSPPPSHPLKAVPIAVADEGESESEDDDLK PRGLTGMKNLGNSCYMNAALQALSNCPPLTQFFLECGGLVRTDKKPALCKSYQKLVSEVWHKKRPSYVVP TSLSHGIKLVNPMFRGYAQQDTQEFLRCLMDQLHEELKEPVVATVALTEARDSDSSDTDEKREGDRSPSE DEFLSCDSSSDRGEGDGQGRGGGSSQAETELLIPDEAGRAISEKERMKDRKFSWGQQRTNSEQVDEDADV DTAMAALDQPAEAQPPSPRSSSPCRTPEPDNDAHLRSSSRPCSPVHHHEGHAKLSSSPPRASPVRMAPSY VLKKAQVLSAGSRRRKEQRYRSVISDIFDGSILSLVQCLTCDRVSTTVETFQDLSLPIPGKEDLAKLHSA IYQNVPAKPGACGDSYAAQGWLAFIVEYIRRFVVSCTPSWFWGPVVTLEDCLAAFFAADELKGDNMYSCE RCKKLRNGVKYCKVLRLPEILCIHLKRFRHEVMYSFKINSHVSFPLEGLDLRPFLAKECTSQITTYDLLS VICHHGTAGSGHYIAYCQNVINGQWYEFDDQYVTEVHETVVQNAEGYVLFYRKSSEEAMRERQQVVSLAA MREPSLLRFYVSREWLNKFNTFAEPGPITNQTFLCSHGGIPPHKYHYIDDLVVILPQNVWEHLYNRFGGG PAVNHLYVCSICQVEIEALAKRRRIEIDTFIKLNKAFQAEESPGVIYCISMQWFREWEAFVKGKDNEPPG PIDNSRIAQVKGSGHVQLKQGADYGQISEETWTYLNSLYGGGPEIAIRQSVAQPLGPENLHGEQKIEAET RAV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001008563 |
| ORF Size | 2739 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001008563.2 |
| RefSeq Size | 4459 bp |
| RefSeq ORF | 2745 bp |
| Locus ID | 10868 |
| UniProt ID | Q9Y2K6 |
| Protein Families | Druggable Genome, Protease |
| MW | 101.9 kDa |
| Gene Summary | This gene encodes a ubiquitin specific processing protease that was first identified as a substrate of the VHL (von Hippel-Lindau disease) protein E3 ubiquitin ligase complex. In addition to being ubiquitinated by the VHL-E3 ligase complex, this enzyme deubiquitinates hypoxia-inducible factor (HIF)-1 alpha and thereby causes increased expression of HIF-1alpha targeted genes which play a role in angiogenesis, glucose metabolism, cell proliferation and metastasis. The enzyme encoded by this gene also regulates G-protein coupled receptor signaling by mediating the deubiquitination of beta-2 adrenergic receptor (ADRB2). This enzyme is a ubiquitously expressed thiolester hydrolase. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq, Jan 2013] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC213090L3 | Lenti ORF clone of Human ubiquitin specific peptidase 20 (USP20), transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC213090L4 | Lenti ORF clone of Human ubiquitin specific peptidase 20 (USP20), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
| RG213090 | USP20 (tGFP-tagged) - Human ubiquitin specific peptidase 20 (USP20), transcript variant 2 |
CNY 8,288.00 |
|
| SC301324 | USP20 (untagged)-Human ubiquitin specific peptidase 20 (USP20), transcript variant 2 |
CNY 7,220.00 |
|
| SC328026 | USP20 (untagged)-Human ubiquitin specific peptidase 20 (USP20) transcript variant 2 |
CNY 7,220.00 |
