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DLC1 (NM_006094) Human Tagged ORF Clone
CAT＃: RC213320
DLC1 (Myc-DDK-tagged)-Human deleted in liver cancer 1 (DLC1), transcript variant 2
ORF Plasmid: tGFP
"NM_006094" in other vectors (4)
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CNY 7,600.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | ARHGAP7; HP; p122-RhoGAP; STARD12 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC213320 representing NM_006094
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTGCAGAAAGAAGCCGGACACCATGATCCTAACACAAATTGAAGCCAAGGAAGCTTGTGATTGGCTAC GGGCAACTGGTTTCCCCCAGTATGCACAGCTTTATGAAGATTTCCTGTTCCCCATCGATATTTCCTTGGT CAAGAGAGAGCATGATTTTTTGGACAGAGATGCCATTGAGGCTCTATGCAGGCGTCTAAATACTTTAAAC AAATGTGCGGTGATGAAGCTAGAAATTAGTCCTCATCGGAAACGAAGTGACGATTCAGACGAGGATGAGC CTTGTGCCATCAGTGGCAAATGGACTTTCCAAAGGGACAGCAAGAGGTGGTCCCGGCTTGAAGAGTTTGA TGTCTTTTCTCCAAAACAAGACCTGGTCCCTGGGTCCCCAGACGACTCCCACCCGAAGGACGGCCCCAGC CCCGGAGGCACGCTGATGGACCTCAGCGAGCGCCAGGAGGTGTCTTCCGTCCGCAGCCTCAGCAGCACTG GCAGCCTCCCCAGCCACGCGCCCCCCAGCGAGGATGCTGCCACCCCCCGGACTAACTCCGTCATCAGCGT TTGCTCCTCCAGCAACTTGGCAGGCAATGACGACTCTTTCGGCAGCCTGCCCTCTCCCAAGGAACTGTCC AGCTTCAGCTTCAGCATGAAAGGCCACGAAAAAACTGCCAAGTCCAAGACGCGCAGTCTGCTGAAACGGA TGGAGAGCCTGAAGCTCAAGAGCTCCCATCACAGCAAGCACAAAGCGCCCTCAAAGCTGGGGTTGATCAT CAGCGGGCCCATCTTGCAAGAGGGGATGGATGAGGAGAAGCTGAAGCAGCTCAACTGCGTGGAGATCTCC GCCCTCAATGGCAACCGCATCAACGTCCCCATGGTACGAAAGAGGAGCGTTTCCAACTCCACGCAGACCA GCAGCAGCAGCAGCCAGTCGGAGACCAGCAGCGCGGTCAGCACGCCCAGCCCTGTTACGAGGACCCGGAG CCTCAGTGCGTGCAACAAGCGGGTGGGCATGTACTTAGAGGGCTTCGATCCTTTCAATCAGTCAACATTT AACAACGTGATGGAGCAGAACTTTAAGAACCGCGAGAGCTACCCAGAGGACACGGTGTTCTACATCCCTG AAGATCACAAGCCTGGCACTTTCCCCAAAGCTCTCACCAATGGCAGTTTCTCCCCCTCGGGGAATAACGG CTCTGTGAACTGGAGGACGGGAAGCTTCCACGGCCCTGGCCACATCAGCCTCAGGAGGGAAAACAGTAGC GACAGCCCCAAGGAACTGAAGAGACGCAATTCTTCCAGCTCCATGAGCAGCCGCCTGAGCATCTACGACA ACGTGCCGGGCTCCATCCTCTACTCCAGTTCAGGGGACCTGGCGGATCTGGAGAACGAGGACATCTTCCC CGAGCTGGACGACATCCTCTACCACGTGAAGGGGATGCAGCGGATAGTCAATCAGTGGTCGGAGAAGTTT TCTGATGAGGGAGATTCGGACTCAGCCCTGGACTCGGTCTCTCCCTGCCCGTCCTCTCCAAAACAGATAC ACCTGGATGTGGACAACGACCGAACCACACCCAGCGACCTGGACAGCACAGGCAACTCCCTGAATGAACC GGAAGAGCCCTCCGAGATCCCGGAAAGAAGGGATTCTGGGGTTGGGGCTTCCCTAACCAGGTCCAACAGG CACCGACTGAGATGGCACAGTTTCCAGAGCTCACATCGGCCAAGCCTCAACTCTGTATCACTACAGATTA ACTGCCAGTCTGTGGCCCAGATGAACCTGCTGCAGAAATACTCACTCCTAAAGCTAACGGCCCTGCTGGA GAAATACACACCTTCTAACAAGCATGGTTTTAGCTGGGCCGTGCCCAAGTTCATGAAGAGGATCAAGGTT CCAGACTACAAGGACCGGAGTGTGTTTGGGGTCCCACTGACGGTCAACGTGCAGCGCACAGGACAACCGT TGCCTCAGAGCATCCAGCAGGCCATGCGATACCTCCGGAACCATTGTTTGGATCAGGTTGGGCTCTTCAG AAAATCGGGGGTCAAGTCCCGGATTCAGGCTCTGCGCCAGATGAATGAAGGTGCCATAGACTGTGTCAAC TACGAAGGACAGTCTGCTTATGACGTGGCAGACATGCTGAAGCAGTATTTTCGAGATCTTCCTGAGCCAC TAATGACGAACAAACTCTCGGAAACCTTTCTACAGATCTACCAATATGTGCCCAAGGACCAGCGCCTGCA GGCCATCAAGGCTGCCATCATGCTGCTGCCTGACGAGAACCGGGAGGTTCTGCAGACCCTGCTTTATTTC CTGAGCGATGTCACAGCAGCCGTAAAAGAAAACCAGATGACCCCAACCAACCTGGCCGTGTGCTTAGCGC CTTCCCTCTTCCATCTCAACACCCTGAAGAGAGAGAATTCCTCTCCCAGGGTAATGCAAAGAAAACAAAG TTTGGGCAAACCAGATCAGAAAGATTTGAATGAAAACCTAGCTGCCACTCAAGGGCTGGCCCATATGATC GCCGAGTGCAAGAAGCTTTTCCAGGTTCCCGAGGAAATGAGCCGATGTCGTAATTCCTATACCGAACAAG AGCTGAAGCCCCTCACTCTGGAAGCACTCGGGCACCTGGGTAATGATGACTCAGCTGACTACCAACACTT CCTCCAGGACTGTGTGGATGGCCTGTTTAAAGAAGTCAAAGAGAAGTTTAAAGGCTGGGTCAGCTACTCC ACTTCGGAGCAGGCTGAGCTGTCCTATAAGAAGGTGAGCGAAGGACCCCCTCTGAGGCTTTGGAGGTCAG TCATTGAAGTCCCTGCTGTGCCAGAGGAAATCTTAAAGCGCCTACTTAAAGAACAGCACCTCTGGGATGT AGACCTGTTGGATTCAAAAGTGATCGAAATTCTGGACAGCCAAACTGAAATTTACCAGTATGTCCAAAAC AGTATGGCACCTCATCCTGCTCGAGACTACGTTGTTTTAAGAACCTGGAGGACTAATTTACCCAAAGGAG CCTGTGCCCTTTTACTAACCTCTGTGGATCACGATCGCGCACCTGTGGTGGGTGTGAGGGTTAATGTGCT CTTGTCCAGGTATTTGATTGAACCCTGTGGGCCAGGAAAATCCAAACTCACCTACATGTGCAGAGTTGAC TTAAGGGGCCACATGCCAGAATGGTACACAAAATCTTTTGGACATTTGTGTGCAGCTGAAGTTGTAAAGA TCCGGGATTCCTTCAGTAACCAGAACACTGAAACCAAAGACACCAAATCTAGG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC213320 representing NM_006094
Red=Cloning site Green=Tags(s) MCRKKPDTMILTQIEAKEACDWLRATGFPQYAQLYEDFLFPIDISLVKREHDFLDRDAIEALCRRLNTLN KCAVMKLEISPHRKRSDDSDEDEPCAISGKWTFQRDSKRWSRLEEFDVFSPKQDLVPGSPDDSHPKDGPS PGGTLMDLSERQEVSSVRSLSSTGSLPSHAPPSEDAATPRTNSVISVCSSSNLAGNDDSFGSLPSPKELS SFSFSMKGHEKTAKSKTRSLLKRMESLKLKSSHHSKHKAPSKLGLIISGPILQEGMDEEKLKQLNCVEIS ALNGNRINVPMVRKRSVSNSTQTSSSSSQSETSSAVSTPSPVTRTRSLSACNKRVGMYLEGFDPFNQSTF NNVMEQNFKNRESYPEDTVFYIPEDHKPGTFPKALTNGSFSPSGNNGSVNWRTGSFHGPGHISLRRENSS DSPKELKRRNSSSSMSSRLSIYDNVPGSILYSSSGDLADLENEDIFPELDDILYHVKGMQRIVNQWSEKF SDEGDSDSALDSVSPCPSSPKQIHLDVDNDRTTPSDLDSTGNSLNEPEEPSEIPERRDSGVGASLTRSNR HRLRWHSFQSSHRPSLNSVSLQINCQSVAQMNLLQKYSLLKLTALLEKYTPSNKHGFSWAVPKFMKRIKV PDYKDRSVFGVPLTVNVQRTGQPLPQSIQQAMRYLRNHCLDQVGLFRKSGVKSRIQALRQMNEGAIDCVN YEGQSAYDVADMLKQYFRDLPEPLMTNKLSETFLQIYQYVPKDQRLQAIKAAIMLLPDENREVLQTLLYF LSDVTAAVKENQMTPTNLAVCLAPSLFHLNTLKRENSSPRVMQRKQSLGKPDQKDLNENLAATQGLAHMI AECKKLFQVPEEMSRCRNSYTEQELKPLTLEALGHLGNDDSADYQHFLQDCVDGLFKEVKEKFKGWVSYS TSEQAELSYKKVSEGPPLRLWRSVIEVPAVPEEILKRLLKEQHLWDVDLLDSKVIEILDSQTEIYQYVQN SMAPHPARDYVVLRTWRTNLPKGACALLLTSVDHDRAPVVGVRVNVLLSRYLIEPCGPGKSKLTYMCRVD LRGHMPEWYTKSFGHLCAAEVVKIRDSFSNQNTETKDTKSR TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_006094 |
| ORF Size | 3273 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_006094.5 |
| RefSeq Size | 6044 bp |
| RefSeq ORF | 3276 bp |
| Locus ID | 10395 |
| UniProt ID | Q96QB1 |
| Domains | RhoGAP, START |
| MW | 122.6 kDa |
| Gene Summary | This gene encodes a GTPase-activating protein (GAP) that is a member of the rhoGAP family of proteins which play a role in the regulation of small GTP-binding proteins. GAP family proteins participate in signaling pathways that regulate cell processes involved in cytoskeletal changes. This gene functions as a tumor suppressor gene in a number of common cancers, including prostate, lung, colorectal, and breast cancers. Multiple transcript variants due to alternative promoters and alternative splicing have been found for this gene.[provided by RefSeq, Apr 2010] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC213320L3 | Lenti ORF clone of Human deleted in liver cancer 1 (DLC1), transcript variant 2, Myc-DDK-tagged |
CNY 12,830.00 |
|
| RC213320L4 | Lenti ORF clone of Human deleted in liver cancer 1 (DLC1), transcript variant 2, mGFP tagged |
CNY 12,830.00 |
|
| RG213320 | DLC1 (tGFP-tagged) - Human deleted in liver cancer 1 (DLC1), transcript variant 2 |
CNY 9,200.00 |
|
| SC116320 | DLC1 (untagged)-Human deleted in liver cancer 1 (DLC1), transcript variant 2 |
CNY 7,608.00 |
