ORP1 (OSBPL1A) (NM_080597) Human Tagged ORF Clone

CAT#: RC213441

OSBPL1A (Myc-DDK-tagged)-Human oxysterol binding protein-like 1A (OSBPL1A), transcript variant OSBPL1B

ORF Plasmid: DDK tGFP



  "NM_080597" in other vectors (6)

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CNY 6,960.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


Rabbit Polyclonal antibody to ORP1 (oxysterol binding protein-like 1A)
    • 100 ul

CNY 6,281.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms ORP-1; ORP1; OSBPL1B
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC213441 representing NM_080597
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAACACAGAAGCGGAGCAACAGCTTCTCCATCACGCCAGAAATGGCAATGCTGAAGAAGTAAGACAAC
TATTAGAGACCATGGCGAGGAATGAAGTGATTGCTGACATTAATTGCAAAGGAAGAAGTAAGTCTAACTT
GGGCTGGACACCTCTACATCTGGCATGCTATTTTGGACACAGACAAGTGGTCCAGGATCTGTTGAAGGCT
GGTGCAGAAGTGAATGTGTTGAATGACATGGGAGACACGCCGCTTCATCGAGCTGCCTTTACAGGACGAA
AGGAGTTGGTAATGCTTCTCTTAGAATATAATGCTGATACTACTATTGTTAATGGGAGTGGACAGACAGC
AAAAGAAGTTACTCATGCTGAAGAAATCAGAAGCATGCTTGAAGCTGTAGAAAGGACTCAACAAAGAAAG
CTTGAAGAATTACTTTTAGCAGCAGCAAGAGAAGGCAAAACAACAGAACTCACAGCTCTGCTCAACAGGC
CCAATCCTCCTGATGTTAACTGTTCGGATCAGTTAGGAAATACACCCTTGCATTGTGCAGCTTACCGGGC
CCATAAACAATGTGCCTTAAAGCTTCTAAGAAGTGGAGCAGACCCTAATCTGAAGAACAAAAATGATCAG
AAACCTCTTGACCTTGCCCAGGGTGCTGAAATGAAACACATTCTTGTTGGTAATAAGGTCATCTACAAAG
CATTGAAACGATATGAGGGCCCTCTCTGGAAGAGTTCAAGATTTTTTGGCTGGAGATTATTCTGGGTAGT
GTTAGAGCATGGAGTCCTTTCATGGTATAGGAAACAGCCTGATGCAGTTCATAATATTTATCGCCAGGGA
TGCAAACACCTGACTCAAGCAGTATGCACGGTAAAATCCACTGATAGCTGCCTCTTCTTTATTAAATGCT
TTGATGACACCATTCATGGCTTCCGGGTTCCTAAGAATAGCCTTCAGCAGTCAAGAGAGGACTGGCTGGA
AGCAATAGAAGAACATTCTGCTTACAGCACTCACTACTGTTCCCAGGACCAGCTGACTGATGAGGAGGAG
GAAGATACGGTTTCTGCTGCAGACCTGAAGAAATCATTAGAGAAAGCACAGTCATGCCAACAGCGACTAG
ATAGGGAAATTTCCAACTTTCTCAAAATGATTAAGGAGTGTGACATGGCTAAAGAAATGCTTCCATCATT
TCTTCAGAAAGTTGAAGTTGTCTCAGAAGCTTCTAGAGAAACTTGTGTAGCTTTGACTGATTGCCTTAAT
CTCTTCACCAAACAAGAAGGGGTGAGGAATTTTAAATTGGAACAAGAGCAAGAAAAAAACAAAATCTTGT
CAGAAGCACTGGAGACGCTGGCCACTGAACATCATGAATTAGAGCAGTCTCTGGTGAAAGGCTCTCCACC
CGCCAGCATCCTTAGCGAGGACGAGTTCTATGATGCGCTGTCAGATTCCGAGTCCGAAAGGTCCCTGAGT
AGATTGGAAGCAGTGACAGCACGCTCCTTTGAAGAGGAAGGAGAGCATTTGGGCAGTAGAAAACACAGAA
TGTCCGAAGAAAAAGACTGTGGTGGCGGAGATGCTCTCTCCAATGGCATCAAGAAACACAGAACAAGTTT
GCCTTCTCCTATGTTTTCCAGAAATGACTTCAGTATCTGGAGCATCCTCAGAAAATGTATTGGAATGGAA
CTATCCAAGATCACGATGCCAGTTATATTTAATGAGCCTCTGAGCTTCCTACAGCGCCTAACTGAATACA
TGGAGCATACTTACCTCATCCACAAGGCCAGTTCACTCTCTGATCCTGTGGAAAGGATGCAGTGTGTAGC
TGCGTTTGCTGTATCTGCTGTTGCTTCTCAGTGGGAACGGACTGGAAAACCTTTCAACCCACTGCTGGGA
GAGACTTATGAATTAGTGCGAGATGACCTTGGATTTAGACTCATCTCCGAACAGGTCAGCCATCACCCAC
CAATCAGTGCATTTCATGCTGAAGGATTAAACAATGACTTCATCTTTCATGGCTCTATCTATCCCAAACT
GAAATTCTGGGGGAAGAGTGTAGAAGCAGAACCCAAAGGAACCATCACCTTGGAGCTCCTTGAACACAAT
GAGGCATATACATGGACAAATCCCACCTGCTGTGTGCATAATATCATTGTGGGTAAACTGTGGATCGAAC
AGTATGGCAATGTGGAAATTATAAACCACAAGACTGGGGACAAATGTGTGTTGAATTTTAAGCCATGTGG
CCTTTTTGGTAAGGAATTACACAAAGTTGAAGGCTACATTCAAGATAAAAGCAAAAAGAAGCTCTGTGCC
CTCTATGGGAAGTGGACTGAATGTTTATACAGTGTTGACCCTGCCACGTTTGACGCTTACAAAAAAAATG
ATAAGAAAAATACAGAAGAGAAGAAGAACAGCAAACAGATGAGCACCTCTGAGGAGTTGGATGAAATGCC
AGTGCCGGATTCTGAAAGTGTATTCATTATCCCTGGAAGCGTTCTTCTATGGCGAATAGCCCCACGGCCT
CCAAATTCTGCCCAGATGTATAATTTTACTAGTTTTGCAATGGTTTTGAATGAAGTAGACAAAGACATGG
AGAGTGTGATTCCCAAGACAGACTGCAGGTTACGGCCTGACATCAGAGCCATGGAAAATGGAGAGATAGA
TCAAGCTAGTGAAGAAAAAAAACGACTTGAGGAAAAACAAAGAGCAGCCCGCAAAAACAGGTCCAAGTCA
GAAGAGGACTGGAAGACGAGGTGGTTCCATCAAGGTCCTAATCCCTACAATGGAGCACAGGACTGGATTT
ACTCTGGCAGCTACTGGGACAGAAATTACTTCAATTTGCCTGACATTTAT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC213441 representing NM_080597
Red=Cloning site Green=Tags(s)

MNTEAEQQLLHHARNGNAEEVRQLLETMARNEVIADINCKGRSKSNLGWTPLHLACYFGHRQVVQDLLKA
GAEVNVLNDMGDTPLHRAAFTGRKELVMLLLEYNADTTIVNGSGQTAKEVTHAEEIRSMLEAVERTQQRK
LEELLLAAAREGKTTELTALLNRPNPPDVNCSDQLGNTPLHCAAYRAHKQCALKLLRSGADPNLKNKNDQ
KPLDLAQGAEMKHILVGNKVIYKALKRYEGPLWKSSRFFGWRLFWVVLEHGVLSWYRKQPDAVHNIYRQG
CKHLTQAVCTVKSTDSCLFFIKCFDDTIHGFRVPKNSLQQSREDWLEAIEEHSAYSTHYCSQDQLTDEEE
EDTVSAADLKKSLEKAQSCQQRLDREISNFLKMIKECDMAKEMLPSFLQKVEVVSEASRETCVALTDCLN
LFTKQEGVRNFKLEQEQEKNKILSEALETLATEHHELEQSLVKGSPPASILSEDEFYDALSDSESERSLS
RLEAVTARSFEEEGEHLGSRKHRMSEEKDCGGGDALSNGIKKHRTSLPSPMFSRNDFSIWSILRKCIGME
LSKITMPVIFNEPLSFLQRLTEYMEHTYLIHKASSLSDPVERMQCVAAFAVSAVASQWERTGKPFNPLLG
ETYELVRDDLGFRLISEQVSHHPPISAFHAEGLNNDFIFHGSIYPKLKFWGKSVEAEPKGTITLELLEHN
EAYTWTNPTCCVHNIIVGKLWIEQYGNVEIINHKTGDKCVLNFKPCGLFGKELHKVEGYIQDKSKKKLCA
LYGKWTECLYSVDPATFDAYKKNDKKNTEEKKNSKQMSTSEELDEMPVPDSESVFIIPGSVLLWRIAPRP
PNSAQMYNFTSFAMVLNEVDKDMESVIPKTDCRLRPDIRAMENGEIDQASEEKKRLEEKQRAARKNRSKS
EEDWKTRWFHQGPNPYNGAQDWIYSGSYWDRNYFNLPDIY

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_080597
ORF Size 2850 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_080597.4
RefSeq Size 4165 bp
RefSeq ORF 2853 bp
Locus ID 114876
UniProt ID Q9BXW6
Domains Oxysterol_BP, PH, ANK
MW 108.3 kDa
Gene Summary This gene encodes a member of the oxysterol-binding protein (OSBP) family, a group of intracellular lipid receptors. Most members contain an N-terminal pleckstrin homology domain and a highly conserved C-terminal OSBP-like sterol-binding domain, although some members contain only the sterol-binding domain. Transcript variants derived from alternative promoter usage and/or alternative splicing exist; they encode different isoforms. [provided by RefSeq, Jul 2008]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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