GP210 (NUP210) (NM_024923) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	GP210; POM210
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC213658 representing NM_024923 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCGCGGGGCCGGGGGCTGCTGCTGCTGACGCTGTCGGTGCTGTTGGCGGCGGGCCCCTCCGCCG CTGCGGCCAAGCTCAACATCCCCAAAGTGCTGCTGCCCTTCACGCGGGCCACGCGCGTTAACTTCACGCT GGAGGCCTCGGAGGGCTGCTACCGCTGGTTGTCCACCCGGCCGGAGGTGGCCAGCATCGAGCCGCTGGGC CTGGACGAGCAGCAGTGCTCCCAGAAGGCAGTGGTGCAGGCCCGCCTGACCCAGCCTGCCCGCCTCACCA GCATCATCTTCGCAGAGGACATCACCACAGGCCAGGTCCTGCGCTGTGATGCCATTGTGGACCTCATCCA TGACATCCAGATCGTCTCCACCACCCGCGAGCTCTACCTGGAGGACTCCCCCCTGGAGCTGAAGATCCAG GCCCTGGACTCCGAAGGGAACACCTTCAGCACTCTGGCTGGACTGGTCTTCGAGTGGACGATTGTGAAGG ACTCCGAGGCGGACAGGTTCTCAGACTCCCACAATGCGCTGCGAATCCTCACTTTCTTGGAGTCTACGTA CATCCCTCCTTCTTACATCTCAGAGATGGAGAAGGCTGCCAAGCAAGGGGACACCATCCTGGTGTCTGGG ATGAAGACCGGGAGCTCCAAGCTCAAGGCTCGCATCCAGGAGGCTGTCTACAAGAATGTACGCCCTGCAG AAGTCAGGCTGCTGATTTTGGAAAACATCCTTCTGAACCCGGCCTATGACGTCTACCTGATGGTGGGAAC CTCCATTCACTACAAGGTGCAGAAGATCAGGCAAGGGAAAATTACAGAACTCTCCATGCCTTCCGATCAG TACGAGTTGCAGCTTCAGAACAGCATCCCGGGCCCCGAAGGAGACCCAGCCCGGCCGGTGGCTGTCTTGG CCCAGGACACGTCGATGGTCACTGCACTGCAGCTGGGACAGAGCAGCCTCGTCCTTGGCCACAGGAGTAT TCGCATGCAAGGTGCTTCTAGGTTACCCAACAGCACTATCTACGTGGTCGAACCTGGATACCTAGGGTTC ACTGTTCACCCTGGTGACAGGTGGGTGCTGGAGACCGGCCGCCTGTATGAAATCACCATCGAAGTTTTTG ACAAGTTCAGCAACAAGGTCTATGTATCTGACAACATCCGAATTGAAACTGTGCTTCCTGCTGAGTTCTT CGAGGTGCTCTCGTCCTCCCAGAATGGGTCATACCATCGCATCAGGGCACTAAAGAGGGGACAGACGGCC ATTGACGCGGCCCTCACCTCTGTGGTGGACCAGGATGGAGGGGTCCACATACTACAGGTGCCTGTGTGGA ACCAGCAGGAGGTGGAAATTCACATCCCGATCACCCTGTATCCCAGCATCTTGACATTTCCGTGGCAACC AAAGACGGGCGCCTATCAGTACACAATAAGGGCCCACGGTGGCAGTGGGAACTTCAGCTGGTCTTCGTCA AGCCACCTGGTTGCCACAGTTACTGTCAAGGGCGTGATGACCACAGGCAGTGACATCGGGTTCAGTGTGA TCCAGGCACATGATGTGCAGAACCCACTCCATTTCGGTGAGATGAAGGTGTATGTGATCGAGCCCCACAG CATGGAGTTTGCCCCGTGCCAGGTGGAGGCACGTGTGGGCCAGGCCCTGGAGCTGCCCCTGAGGATCAGT GGCCTCATGCCCGGCGGGGCCAGTGAGGTGGTCACCTTGAGCGACTGCTCCCACTTTGACTTGGCTGTCG AGGTGGAGAACCAGGGTGTGTTCCAGCCACTCCCAGGGAGGCTGCCGCCAGGCTCTGAGCACTGCAGCGG CATCCGGGTAAAGGCCGAGGCCCAGGGCTCTACCACGCTTCTTGTGAGCTACAGACACGGCCACGTCCAC CTGAGTGCCAAGATCACCATTGCTGCCTACCTGCCCCTCAAGGCTGTGGATCCCTCCTCTGTTGCCTTGG TAACCCTGGGCTCCTCAAAGGAGATGCTGTTTGAAGGAGGTCCCAGACCTTGGATCCTCGAGCCGTCCAA ATTCTTCCAGAACGTCACCGCTGAGGACACTGACAGCATCGGCCTGGCTCTCTTTGCCCCCCATTCCTCC CGGAATTATCAGCAACACTGGATCCTTGTGACCTGTCAGGCCTTGGGTGAGCAGGTCATCGCCCTGTCGG TGGGGAACAAGCCCAGCCTCACCAACCCCTTTCCTGCGGTGGAGCCTGCCGTGGTGAAGTTCGTCTGCGC CCCACCGTCCAGGCTCACCCTCGCGCCTGTCTACACCAGCCCCCAGCTGGACATGTCCTGTCCGCTGCTG CAGCAGAACAAGCAGGTGGTCCCAGTGTCCAGCCACCGCAACCCCCGGCTGGACCTGGCTGCTTACGACC AGGAGGGCCGCCGGTTCGACAACTTCAGCTCTCTGAGCATCCAGTGGGAGTCCACCAGGCCAGTGTTGGC CAGCATCGAGCCTGAGCTGCCCATGCAGCTGGTGTCCCAGGACGATGAGAGTGGCCAAAAGAAGCTGCAC GGTTTGCAGGCCATTTTGGTTCACGAGGCATCAGGAACCACAGCCATCACTGCCACTGCCACTGGCTACC AGGAGTCCCACCTCAGCTCTGCCAGAACAAAGCAGCCGCATGACCCTCTGGTGCCTCTGTCGGCCTCCAT AGAGCTCATCCTGGTGGAGGACGTGAGGGTGAGCCCAGAAGAGGTGACCATCTACAACCACCCTGGCATC CAGGCAGAGCTCCGCATCAGGGAAGGCTCAGGTTACTTCTTCCTCAACACCAGCACCGCAGATGTTGTCA AGGTGGCCTACCAGGAGGCCAGGGGTGTCGCCATGGTGCACCCTTTGCTCCCGGGCTCATCCACCATCAT GATCCATGACTTGTGCCTCGTCTTCCCGGCCCCAGCCAAGGCTGTCGTTTACGTGTCGGACATTCAGGAG CTGTACATCCGTGTGGTTGACAAGGTGGAGATTGGGAAGACAGTGAAGGCATACGTCCGCGTGCTGGACT TGCACAAGAAGCCCTTCCTTGCCAAATACTTCCCCTTTATGGACCTGAAGCTCCGAGCAGCCTCCCCGAT CATTACATTGGTGGCCCTTGATGAAGCCCTTGACAACTACACCATCACATTCCTCATCCGCGGTGTGGCC ATCGGCCAGACCAGTCTAACTGCAAGTGTGACCAATAAAGCTGGACAGAGAATCAACTCAGCCCCACAAC AGATTGAAGTCTTTCCCCCGTTCAGGCTGATGCCCAGGAAGGTGACACTGCTTATCGGGGCCACGATGCA GGTCACCTCCGAGGGCGGCCCCCAGCCTCAGTCCAACATCCTTTTCTCCATCAGCAATGAGAGCGTTGCG CTGGTGAGCGCTGCTGGGCTGGTACAGGGCCTCGCCATCGGGAACGGCACTGTGTCTGGGCTCGTGCAGG CAGTGGATGCAGAGACCGGCAAGGTGGTCATCATCTCTCAGGACCTCGTGCAGGTGGAGGTGCTGCTGCT AAGGGCCGTGAGGATCCGCGCCCCCATCATGCGGATGAGGACGGGCACCCAGATGCCCATCTATGTCACC GGCATCACCAACCACCAGAACCCTTTCTCCTTTGGCAATGCCGTGCCAGGCCTGACCTTCCACTGGTCTG TCACCAAGCGGGACGTCCTGGACCTCCGAGGGCGGCACCACGAGGCGTCGATCCGACTCCCGTCACAGTA CAACTTTGCCATGAACGTGCTCGGCCGGGTAAAAGGCCGGACCGGGCTGAGGGTGGTGGTCAAGGCTGTG GACCCCACATCGGGGCAGCTGTATGGCCTGGCCAGAGAACTCTCGGATGAGATCCAAGTCCAGGTGTTTG AGAAGCTGCAGCTGCTCAACCCTGAAATAGAAGCAGAACAAATATTAATGTCGCCCAACTCATATATAAA GCTGCAGACAAACAGGGATGGTGCAGCCTCTCTGAGCTACCGCGTCCTGGATGGACCCGAAAAGGTTCCA GTTGTGCATGTTGATGAGAAAGGCTTTCTAGCATCAGGGTCTATGATCGGGACATCCACCATCGAAGTGA TTGCACAAGAGCCCTTTGGGGCCAACCAAACCATCATTGTTGCTGTAAAGGTATCCCCTGTTTCCTACCT GAGGGTTTCCATGAGCCCTGTCCTGCACACCCAGAACAAGGAGGCCCTGGTGGCCGTGCCTTTGGGAATG ACCGTGACCTTCACTGTCCACTTCCACGACAACTCTGGAGATGTCTTCCATGCTCACAGTTCGGTCCTCA ACTTTGCCACTAACAGAGACGACTTTGTGCAGATCGGGAAGGGCCCCACCAACAACACCTGCGTTGTCCG CACAGTCAGCGTGGGCCTGACACTGCTCCGTGTGTGGGACGCAGAGCACCCGGGCCTCTCGGACTTCATG CCCCTGCCTGTCCTACAGGCCATCTCCCCAGAGCTGTCTGGGGCCATGGTGGTGGGGGACGTGCTCTGTC TGGCCACTGTTCTGACCAGCCTGGAAGGCCTCTCAGGAACCTGGAGCTCCTCAGCCAACAGCATCCTCCA CATCGACCCCAAGACGGGTGTGGCTGTGGCCCGGGCCGTGGGATCCGTGACGGTTTACTATGAGGTCGCT GGGCACCTGAGGACCTACAAGGAGGTGGTGGTCAGCGTCCCTCAGAGGATCATGGCCCGTCACCTCCACC CCATCCAGACCAGCTTCCAGGAGGCTACAGCCTCCAAAGTGATTGTTGCCGTGGGAGACAGAAGCTCTAA CCTGAGAGGCGAGTGCACCCCCACCCAGAGGGAAGTCATCCAGGCCTTGCACCCAGAGACCCTCATCAGC TGCCAGTCCCAGTTCAAGCCGGCCGTCTTTGATTTCCCATCTCAAGATGTGTTCACCGTGGAGCCACAGT TTGACACTGCTCTCGGCCAGTACTTCTGCTCAATCACAATGCACAGGCTGACGGACAAGCAGCGGAAGCA CCTGAGCATGAAGAAGACAGCTCTGGTGGTCAGTGCCTCCCTCTCCAGCAGCCACTTCTCCACAGAGCAG GTGGGGGCCGAGGTGCCCTTCAGCCCAGGTCTCTTCGCCGACCAGGCTGAAATCCTTTTGAGCAACCACT ACACCAGTTCCGAGATCAGGGTCTTTGGTGCCCCGGAGGTTCTGGAGAACTTGGAGGTGAAATCCGGGTC CCCGGCCGTGCTGGCATTCGCAAAGGAGAAGTCTTTTGGGTGGCCCAGCTTCATCACATACACGGTCGGC GTCTTGGACCCCGCGGCTGGCAGCCAAGGGCCTCTGTCCACTACCCTGACCTTCTCCAGCCCCGTGACCA ACCAAGCCATTGCCATCCCAGTGACAGTGGCTTTTGTGGTGGATCGCCGTGGGCCCGGTCCTTATGGAGC CAGCCTCTTCCAGCACTTCCTGGATTCCTACCAGGTCATGTTCTTCACGCTCTTCGCCCTGTTGGCTGGG ACAGCGGTCATGATCATAGCCTACCACACTGTCTGCACGCCCCGGGATCTTGCTGTGCCTGCAGCCCTCA CGCCTCGAGCCAGCCCTGGACACAGCCCCCACTATTTCGCTGCCTCATCACCCACATCTCCCAATGCATT GCCTCCTGCTCGCAAAGCCAGCCCTCCCTCAGGGCTGTGGAGCCCAGCCTATGCCTCCCAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC213658 representing NM_024923 Red=Cloning site Green=Tags(s) MAARGRGLLLLTLSVLLAAGPSAAAAKLNIPKVLLPFTRATRVNFTLEASEGCYRWLSTRPEVASIEPLG LDEQQCSQKAVVQARLTQPARLTSIIFAEDITTGQVLRCDAIVDLIHDIQIVSTTRELYLEDSPLELKIQ ALDSEGNTFSTLAGLVFEWTIVKDSEADRFSDSHNALRILTFLESTYIPPSYISEMEKAAKQGDTILVSG MKTGSSKLKARIQEAVYKNVRPAEVRLLILENILLNPAYDVYLMVGTSIHYKVQKIRQGKITELSMPSDQ YELQLQNSIPGPEGDPARPVAVLAQDTSMVTALQLGQSSLVLGHRSIRMQGASRLPNSTIYVVEPGYLGF TVHPGDRWVLETGRLYEITIEVFDKFSNKVYVSDNIRIETVLPAEFFEVLSSSQNGSYHRIRALKRGQTA IDAALTSVVDQDGGVHILQVPVWNQQEVEIHIPITLYPSILTFPWQPKTGAYQYTIRAHGGSGNFSWSSS SHLVATVTVKGVMTTGSDIGFSVIQAHDVQNPLHFGEMKVYVIEPHSMEFAPCQVEARVGQALELPLRIS GLMPGGASEVVTLSDCSHFDLAVEVENQGVFQPLPGRLPPGSEHCSGIRVKAEAQGSTTLLVSYRHGHVH LSAKITIAAYLPLKAVDPSSVALVTLGSSKEMLFEGGPRPWILEPSKFFQNVTAEDTDSIGLALFAPHSS RNYQQHWILVTCQALGEQVIALSVGNKPSLTNPFPAVEPAVVKFVCAPPSRLTLAPVYTSPQLDMSCPLL QQNKQVVPVSSHRNPRLDLAAYDQEGRRFDNFSSLSIQWESTRPVLASIEPELPMQLVSQDDESGQKKLH GLQAILVHEASGTTAITATATGYQESHLSSARTKQPHDPLVPLSASIELILVEDVRVSPEEVTIYNHPGI QAELRIREGSGYFFLNTSTADVVKVAYQEARGVAMVHPLLPGSSTIMIHDLCLVFPAPAKAVVYVSDIQE LYIRVVDKVEIGKTVKAYVRVLDLHKKPFLAKYFPFMDLKLRAASPIITLVALDEALDNYTITFLIRGVA IGQTSLTASVTNKAGQRINSAPQQIEVFPPFRLMPRKVTLLIGATMQVTSEGGPQPQSNILFSISNESVA LVSAAGLVQGLAIGNGTVSGLVQAVDAETGKVVIISQDLVQVEVLLLRAVRIRAPIMRMRTGTQMPIYVT GITNHQNPFSFGNAVPGLTFHWSVTKRDVLDLRGRHHEASIRLPSQYNFAMNVLGRVKGRTGLRVVVKAV DPTSGQLYGLARELSDEIQVQVFEKLQLLNPEIEAEQILMSPNSYIKLQTNRDGAASLSYRVLDGPEKVP VVHVDEKGFLASGSMIGTSTIEVIAQEPFGANQTIIVAVKVSPVSYLRVSMSPVLHTQNKEALVAVPLGM TVTFTVHFHDNSGDVFHAHSSVLNFATNRDDFVQIGKGPTNNTCVVRTVSVGLTLLRVWDAEHPGLSDFM PLPVLQAISPELSGAMVVGDVLCLATVLTSLEGLSGTWSSSANSILHIDPKTGVAVARAVGSVTVYYEVA GHLRTYKEVVVSVPQRIMARHLHPIQTSFQEATASKVIVAVGDRSSNLRGECTPTQREVIQALHPETLIS CQSQFKPAVFDFPSQDVFTVEPQFDTALGQYFCSITMHRLTDKQRKHLSMKKTALVVSASLSSSHFSTEQ VGAEVPFSPGLFADQAEILLSNHYTSSEIRVFGAPEVLENLEVKSGSPAVLAFAKEKSFGWPSFITYTVG VLDPAAGSQGPLSTTLTFSSPVTNQAIAIPVTVAFVVDRRGPGPYGASLFQHFLDSYQVMFFTLFALLAG TAVMIIAYHTVCTPRDLAVPAALTPRASPGHSPHYFAASSPTSPNALPPARKASPPSGLWSPAYASH TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms	CHROMATOGRAMS Sequencher program is needed, download here.
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_024923
ORF Size	5661 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_024923.2, NP_079199.2
RefSeq Size	7191 bp
RefSeq ORF	5664 bp
Locus ID	23225
UniProt ID	Q8TEM1
Protein Families	Transmembrane
MW	204.9 kDa
Gene Summary	The nuclear pore complex is a massive structure that extends across the nuclear envelope, forming a gateway that regulates the flow of macromolecules between the nucleus and the cytoplasm. Nucleoporins are the main components of the nuclear pore complex in eukaryotic cells. The protein encoded by this gene is a membrane-spanning glycoprotein that is a major component of the nuclear pore complex. Multiple pseudogenes related to this gene are located on chromosome 3. [provided by RefSeq, Jul 2013]

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