GP210 (NUP210) (NM_024923) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	GP210; POM210
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC305194 representing NM_024923. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGCGGCGCGGGGCCGGGGGCTGCTGCTGCTGACGCTGTCGGTGCTGTTGGCGGCGGGCCCCTCCGCC GCTGCGGCCAAGCTCAACATCCCCAAAGTGCTGCTGCCCTTCACGCGGGCCACGCGCGTTAACTTCACG CTGGAGGCCTCGGAGGGCTGCTACCGCTGGTTGTCCACCCGGCCGGAGGTGGCCAGCATCGAGCCGCTG GGCCTGGACGAGCAGCAGTGCTCCCAGAAGGCAGTGGTGCAGGCCCGCCTGACCCAGCCTGCCCGCCTC ACCAGCATCATCTTCGCAGAGGACATCACCACAGGCCAGGTCCTGCGCTGTGATGCCATTGTGGACCTC ATCCATGACATCCAGATCGTCTCCACCACCCGCGAGCTCTACCTGGAGGACTCCCCCCTGGAGCTGAAG ATCCAGGCCCTGGACTCCGAAGGGAACACCTTCAGCACTCTGGCTGGACTGGTCTTCGAGTGGACGATT GTGAAGGACTCCGAGGCGGACAGGTTCTCAGACTCCCACAATGCGCTGCGAATCCTCACTTTCTTGGAG TCTACGTACATCCCTCCTTCTTACATCTCAGAGATGGAGAAGGCTGCCAAGCAAGGGGACACCATCCTG GTGTCTGGGATGAAGACCGGGAGCTCCAAGCTCAAGGCTCGCATCCAGGAGGCTGTCTACAAGAATGTA CGCCCTGCAGAAGTCAGGCTGCTGATTTTGGAAAACATCCTTCTGAACCCGGCCTATGACGTCTACCTG ATGGTGGGAACCTCCATTCACTACAAGGTGCAGAAGATCAGGCAAGGGAAAATTACAGAACTCTCCATG CCTTCCGATCAGTACGAGTTGCAGCTTCAGAACAGCATCCCGGGCCCCGAAGGAGACCCAGCCCGGCCG GTGGCTGTCTTGGCCCAGGACACGTCGATGGTCACTGCACTGCAGCTGGGACAGAGCAGCCTCGTCCTT GGCCACAGGAGTATTCGCATGCAAGGTGCTTCTAGGTTACCCAACAGCACTATCTACGTGGTCGAACCT GGATACCTAGGGTTCACTGTTCACCCTGGTGACAGGTGGGTGCTGGAGACCGGCCGCCTGTATGAAATC ACCATCGAAGTTTTTGACAAGTTCAGCAACAAGGTCTATGTATCTGACAACATCCGAATTGAAACTGTG CTTCCTGCTGAGTTCTTCGAGGTGCTCTCGTCCTCCCAGAATGGGTCATACCATCGCATCAGGGCACTA AAGAGGGGACAGACGGCCATTGACGCGGCCCTCACCTCTGTGGTGGACCAGGATGGAGGGGTCCACATA CTACAGGTGCCTGTGTGGAACCAGCAGGAGGTGGAAATTCACATCCCGATCACCCTGTATCCCAGCATC TTGACATTTCCGTGGCAACCAAAGACGGGCGCCTATCAGTACACAATAAGGGCCCACGGTGGCAGTGGG AACTTCAGCTGGTCTTCGTCAAGCCACCTGGTTGCCACAGTTACTGTCAAGGGCGTGATGACCACAGGC AGTGACATCGGGTTCAGTGTGATCCAGGCACATGATGTGCAGAACCCACTCCATTTCGGTGAGATGAAG GTGTATGTGATCGAGCCCCACAGCATGGAGTTTGCCCCGTGCCAGGTGGAGGCACGTGTGGGCCAGGCC CTGGAGCTGCCCCTGAGGATCAGTGGCCTCATGCCCGGCGGGGCCAGTGAGGTGGTCACCTTGAGCGAC TGCTCCCACTTTGACTTGGCTGTCGAGGTGGAGAACCAGGGTGTGTTCCAGCCACTCCCAGGGAGGCTG CCGCCAGGCTCTGAGCACTGCAGCGGCATCCGGGTAAAGGCCGAGGCCCAGGGCTCTACCACGCTTCTT GTGAGCTACAGACACGGCCACGTCCACCTGAGTGCCAAGATCACCATTGCTGCCTACCTGCCCCTCAAG GCTGTGGATCCCTCCTCTGTTGCCTTGGTAACCCTGGGCTCCTCAAAGGAGATGCTGTTTGAAGGAGGT CCCAGACCTTGGATCCTCGAGCCGTCCAAATTCTTCCAGAACGTCACCGCTGAGGACACTGACAGCATC GGCCTGGCTCTCTTTGCCCCCCATTCCTCCCGGAATTATCAGCAACACTGGATCCTTGTGACCTGTCAG GCCTTGGGTGAGCAGGTCATCGCCCTGTCGGTGGGGAACAAGCCCAGCCTCACCAACCCCTTTCCTGCG GTGGAGCCTGCCGTGGTGAAGTTCGTCTGCGCCCCACCGTCCAGGCTCACCCTCGCGCCTGTCTACACC AGCCCCCAGCTGGACATGTCCTGTCCGCTGCTGCAGCAGAACAAGCAGGTGGTCCCAGTGTCCAGCCAC CGCAACCCCCGGCTGGACCTGGCTGCTTACGACCAGGAGGGCCGCCGGTTCGACAACTTCAGCTCTCTG AGCATCCAGTGGGAGTCCACCAGGCCAGTGTTGGCCAGCATCGAGCCTGAGCTGCCCATGCAGCTGGTG TCCCAGGACGATGAGAGTGGCCAAAAGAAGCTGCACGGTTTGCAGGCCATTTTGGTTCACGAGGCATCA GGAACCACAGCCATCACTGCCACTGCCACTGGCTACCAGGAGTCCCACCTCAGCTCTGCCAGAACAAAG CAGCCGCATGACCCTCTGGTGCCTCTGTCGGCCTCCATAGAGCTCATCCTGGTGGAGGACGTGAGGGTG AGCCCAGAAGAGGTGACCATCTACAACCACCCTGGCATCCAGGCAGAGCTCCGCATCAGGGAAGGCTCA GGTTACTTCTTCCTCAACACCAGCACCGCAGATGTTGTCAAGGTGGCCTACCAGGAGGCCAGGGGTGTC GCCATGGTGCACCCTTTGCTCCCGGGCTCATCCACCATCATGATCCATGACTTGTGCCTCGTCTTCCCG GCCCCAGCCAAGGCTGTCGTTTACGTGTCGGACATTCAGGAGCTGTACATCCGTGTGGTTGACAAGGTG GAGATTGGGAAGACAGTGAAGGCATACGTCCGCGTGCTGGACTTGCACAAGAAGCCCTTCCTTGCCAAA TACTTCCCCTTTATGGACCTGAAGCTCCGAGCAGCCTCCCCGATCATTACATTGGTGGCCCTTGATGAA GCCCTTGACAACTACACCATCACATTCCTCATCCGCGGTGTGGCCATCGGCCAGACCAGTCTAACTGCA AGTGTGACCAATAAAGCTGGACAGAGAATCAACTCAGCCCCACAACAGATTGAAGTCTTTCCCCCGTTC AGGCTGATGCCCAGGAAGGTGACACTGCTTATCGGGGCCACGATGCAGGTCACCTCCGAGGGCGGCCCC CAGCCTCAGTCCAACATCCTTTTCTCCATCAGCAATGAGAGCGTTGCGCTGGTGAGCGCTGCTGGGCTG GTACAGGGCCTCGCCATCGGGAACGGCACTGTGTCTGGGCTCGTGCAGGCAGTGGATGCAGAGACCGGC AAGGTGGTCATCATCTCTCAGGACCTCGTGCAGGTGGAGGTGCTGCTGCTAAGGGCCGTGAGGATCCGC GCCCCCATCATGCGGATGAGGACGGGCACCCAGATGCCCATCTATGTCACCGGCATCACCAACCACCAG AACCCTTTCTCCTTTGGCAATGCCGTGCCAGGCCTGACCTTCCACTGGTCTGTCACCAAGCGGGACGTC CTGGACCTCCGAGGGCGGCACCACGAGGCGTCGATCCGACTCCCGTCACAGTACAACTTTGCCATGAAC GTGCTCGGCCGGGTAAAAGGCCGGACCGGGCTGAGGGTGGTGGTCAAGGCTGTGGACCCCACATCGGGG CAGCTGTATGGCCTGGCCAGAGAACTCTCGGATGAGATCCAAGTCCAGGTGTTTGAGAAGCTGCAGCTG CTCAACCCTGAAATAGAAGCAGAACAAATATTAATGTCGCCCAACTCATATATAAAGCTGCAGACAAAC AGGGATGGTGCAGCCTCTCTGAGCTACCGCGTCCTGGATGGACCCGAAAAGGTTCCAGTTGTGCATGTT GATGAGAAAGGCTTTCTAGCATCAGGGTCTATGATCGGGACATCCACCATCGAAGTGATTGCACAAGAG CCCTTTGGGGCCAACCAAACCATCATTGTTGCTGTAAAGGTATCCCCTGTTTCCTACCTGAGGGTTTCC ATGAGCCCTGTCCTGCACACCCAGAACAAGGAGGCCCTGGTGGCCGTGCCTTTGGGAATGACCGTGACC TTCACTGTCCACTTCCACGACAACTCTGGAGATGTCTTCCATGCTCACAGTTCGGTCCTCAACTTTGCC ACTAACAGAGACGACTTTGTGCAGATCGGGAAGGGCCCCACCAACAACACCTGCGTTGTCCGCACAGTC AGCGTGGGCCTGACACTGCTCCGTGTGTGGGACGCAGAGCACCCGGGCCTCTCGGACTTCATGCCCCTG CCTGTCCTACAGGCCATCTCCCCAGAGCTGTCTGGGGCCATGGTGGTGGGGGACGTGCTCTGTCTGGCC ACTGTTCTGACCAGCCTGGAAGGCCTCTCAGGAACCTGGAGCTCCTCGGCCAACAGCATCCTCCACATC GACCCCAAGACGGGTGTGGCTGTGGCCCGGGCCGTGGGATCCGTGACGGTTTACTATGAGGTCGCTGGG CACCTGAGGACCTACAAGGAGGTGGTGGTCAGCGTCCCTCAGAGGATCATGGCCCGTCACCTCCACCCC ATCCAGACCAGCTTCCAGGAGGCTACAGCCTCCAAAGTGATTGTTGCCGTGGGAGACAGAAGCTCTAAC CTGAGAGGCGAGTGCACCCCCACCCAGAGGGAAGTCATCCAGGCCTTGCACCCAGAGACCCTCATCAGC TGCCAGTCCCAGTTCAAGCCGGCCGTCTTTGATTTCCCATCTCAAGATGTGTTCACCGTGGAGCCACAG TTTGACACTGCTCTCGGCCAGTACTTCTGCTCAATCACAATGCACAGGCTGACGGACAAGCAGCGGAAG CACCTGAGCATGAAGAAGACAGCTCTGGTGGTCAGTGCCTCCCTCTCCAGCAGCCACTTCTCCACAGAG CAGGTGGGGGCCGAGGTGCCCTTCAGCCCAGGTCTCTTCGCCGACCAGGCTGAAATCCTTTTGAGCAAC CACTACACCAGTTCCGAGATCAGGGTCTTTGGTGCCCCGGAGGTTCTGGAGAACTTGGAGGTGAAATCC GGGTCCCCGGCCGTGCTGGCATTCGCAAAGGAGAAGTCTTTTGGGTGGCCCAGCTTCATCACATACACG GTCGGCGTCTTGGACCCCGCGGCTGGCAGCCAAGGGCCTCTGTCCACTACCCTGACCTTCTCCAGCCCC GTGACCAACCAAGCCATTGCCATCCCAGTGACAGTGGCTTTTGTGGTGGATCGCCGTGGGCCCGGTCCT TATGGAGCCAGCCTCTTCCAGCACTTCCTGGATTCCTACCAGGTCATGTTCTTCACGCTCTTCGCCCTG TTGGCTGGGACAGCGGTCATGATCATAGCCTACCACACTGTCTGCACGCCCCGGGATCTTGCTGTGCCT GCAGCCCTCACGCCTCGAGCCAGCCCTGGACACAGCCCCCACTATTTCGCTGCCTCATCACCCACATCT CCCAATGCATTGCCTCCTGCTCGCAAAGCCAGCCCTCCCTCAGGGCTGTGGAGCCCAGCCTATGCCTCC CACTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_024923
Insert Size	5664 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_024923.3
RefSeq Size	7218 bp
RefSeq ORF	5664 bp
Locus ID	23225
UniProt ID	Q8TEM1
Protein Families	Transmembrane
MW	205.1 kDa
Gene Summary	The nuclear pore complex is a massive structure that extends across the nuclear envelope, forming a gateway that regulates the flow of macromolecules between the nucleus and the cytoplasm. Nucleoporins are the main components of the nuclear pore complex in eukaryotic cells. The protein encoded by this gene is a membrane-spanning glycoprotein that is a major component of the nuclear pore complex. Multiple pseudogenes related to this gene are located on chromosome 3. [provided by RefSeq, Jul 2013]

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