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ALDH1L1 (NM_012190) Human Tagged ORF Clone
CAT＃: RC213720
ALDH1L1 (Myc-DDK-tagged)-Human aldehyde dehydrogenase 1 family, member L1 (ALDH1L1)
ORF Plasmid: tGFP
"NM_012190" in other vectors (4)
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CNY 6,608.00
CNY 7,130.00
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 10-fTHF; 10-FTHFDH; FDH; FTHFD |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC213720 representing NM_012190
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGATTGCAGTGATTGGACAGAGCCTGTTTGGCCAGGAAGTTTACTGCCACCTGAGGAAGGAGGGCC ACGAAGTGGTGGGTGTGTTCACTGTTCCAGACAAGGATGGAAAGGCCGACCCCCTGGGTCTGGAAGCTGA GAAGGATGGAGTGCCGGTATTCAAGTACTCCCGGTGGCGTGCAAAAGGACAGGCTTTGCCTGATGTGGTG GCAAAATACCAGGCTTTGGGGGCCGAGCTCAACGTCCTGCCCTTCTGCAGCCAATTCATCCCCATGGAGA TAATCAGTGCCCCCCGGCATGGCTCCATCATCTATCACCCGTCACTGCTCCCTAGGCACCGAGGGGCCTC GGCCATCAACTGGACCCTCATTCACGGAGATAAGAAAGGGGGGTTTTCCATCTTCTGGGCGGATGATGGT CTGGACACCGGAGACCTGCTGCTGCAGAAGGAGTGTGAGGTGCTCCCGGACGACACCGTGAGCACGCTGT ACAACCGCTTCCTCTTCCCTGAAGGCATCAAAGGGATGGTGCAGGCCGTGAGGCTGATCGCTGAGGGCAA AGCCCCCAGACTCCCTCAGCCTGAGGAAGGAGCCACCTATGAGGGGATTCAGAAGAAGGAGACAGCCAAG ATCAACTGGGACCAGCCGGCAGAGGCCATTCACAACTGGATCCGCGGGAACGACAAGGTGCCGGGAGCCT GGACAGAGGCCTGTGAACAGAAACTGACATTTTTCAACTCAACGCTGAACACTTCAGGCCTGGTGCCCGA GGGAGACGCTTTGCCCATCCCAGGAGCCCATCGGCCAGGGGTGGTCACCAAAGCAGGACTCATCCTCTTT GGGAATGATGACAAAATGCTGCTGGTGAAGAATATTCAGCTGGAGGATGGCAAAATGATCCTGGCCTCGA ACTTCTTTAAGGGGGCAGCCAGCAGTGTCCTTGAGCTGACAGAGGCAGAGCTGGTTACTGCGGAGGCTGT GCGGAGTTTTTGGCAGCGGATCCTCCCCAAAGTCCTGGAGGTTGAAGACTCCACTGATTTCTTCAAGTCA GGGGCCGCGTCTGTGGACGTTGTGAGGCTGGTGGAGGAAGTGAAGGAGCTGTGTGATGGCCTGGAGTTAG AAAATGAAGATGTGTACATGGCATCCACCTTTGGGGACTTCATCCAGCTGTTAGTGAGGAAGCTTCGAGG GCACGATGAGGAGGGCGAGTGCAGCATTGACTACGTGGAAATGGCAGTGAACAAGCGCACTGTCCGCATG CCCCACCAGCTCTTCATTGGGGGGGAGTTCGTGGATGCCGAGGGCGCCAAGACCTCTGAGACCATCAATC CCACCGATGGAAGTGTCATCTGCCAGGTATCCCTGGCCCAAGTCACCGACGTCGACAAGGCAGTGGCCGC AGCCAAGGATGCCTTTGAGAATGGACGGTGGGGGAAGATCGGTGCGCGGGACCGGGGCCGGCTGATGTAC AGGTTGGCAGATCTCATGGAGCAGCACCAGGAGGAGCTGGCCACCATTGAGGCCCTGGATGCGGGTGCCG TCTACACGCTGGCCCTGAAGACCCACGTGGGCATGTCCATCCAGACCTTCCGCTACTTTGCTGGCTGGTG TGACAAGATCCAGGGCTCCACCATCCCCATCAACCAGGCCAGACCCAACCGCAACCTGACCTTGACCAGG AAGGAGCCTGTTGGGGTTTGTGGCATCATCATCCCCTGGAACTATCCCCTGATGATGCTGTCCTGGAAGA CAGCTGCCTGCCTGGCTGCCGGGAACACAGTGGTGATCAAGCCTGCTCAGGTGACCCCACTCACAGCCTT GAAGTTTGCAGAGCTGACATTAAAGGCCGGCATTCCCAAAGGTGTGGTTAACGTCCTCCCAGGATCTGGC TCCCTGGTCGGCCAGAGACTCTCAGACCATCCTGATGTGAGGAAAATCGGGTTCACAGGCTCCACAGAGG TGGGCAAGCACATCATGAAAAGCTGTGCCATAAGTAACGTGAAGAAGGTGTCCCTGGAACTGGGCGGGAA GTCACCCCTCATCATCTTTGCTGACTGTGACCTCAACAAGGCTGTGCAGATGGGGATGAGTTCTGTTTTC TTCAACAAAGGAGAGAATTGCATTGCAGCAGGCCGACTCTTTGTGGAGGACTCCATTCATGATGAGTTCG TGCGGAGAGTGGTAGAAGAGGTGCGGAAGATGAAGGTGGGCAACCCGCTGGACAGGGACACCGACCACGG GCCGCAGAATCACCATGCCCACCTTGTGAAGCTGATGGAGTACTGCCAGCATGGCGTGAAGGAAGGGGCC ACACTGGTCTGCGGCGGGAATCAGGTCCCTCGGCCAGGGTTCTTCTTTGAGCCAACTGTTTTCACAGACG TGGAAGACCACATGTTCATAGCCAAGGAGGAGTCCTTCGGGCCTGTCATGATCATCTCTCGGTTTGCTGA TGGGGACTTGGATGCCGTGCTGTCTCGGGCCAATGCCACGGAATTTGGCCTGGCTTCTGGTGTCTTCACC AGGGACATCAACAAGGCCCTGTATGTCAGTGACAAGCTCCAGGCAGGCACTGTGTTTGTCAACACGTACA ACAAGACCGACGTGGCCGCTCCCTTCGGAGGATTCAAACAGTCTGGATTTGGCAAAGATCTAGGAGAGGC GGCTCTGAACGAGTACCTGCGGGTCAAGACAGTGACCTTCGAATAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC213720 representing NM_012190
Red=Cloning site Green=Tags(s) MKIAVIGQSLFGQEVYCHLRKEGHEVVGVFTVPDKDGKADPLGLEAEKDGVPVFKYSRWRAKGQALPDVV AKYQALGAELNVLPFCSQFIPMEIISAPRHGSIIYHPSLLPRHRGASAINWTLIHGDKKGGFSIFWADDG LDTGDLLLQKECEVLPDDTVSTLYNRFLFPEGIKGMVQAVRLIAEGKAPRLPQPEEGATYEGIQKKETAK INWDQPAEAIHNWIRGNDKVPGAWTEACEQKLTFFNSTLNTSGLVPEGDALPIPGAHRPGVVTKAGLILF GNDDKMLLVKNIQLEDGKMILASNFFKGAASSVLELTEAELVTAEAVRSFWQRILPKVLEVEDSTDFFKS GAASVDVVRLVEEVKELCDGLELENEDVYMASTFGDFIQLLVRKLRGHDEEGECSIDYVEMAVNKRTVRM PHQLFIGGEFVDAEGAKTSETINPTDGSVICQVSLAQVTDVDKAVAAAKDAFENGRWGKIGARDRGRLMY RLADLMEQHQEELATIEALDAGAVYTLALKTHVGMSIQTFRYFAGWCDKIQGSTIPINQARPNRNLTLTR KEPVGVCGIIIPWNYPLMMLSWKTAACLAAGNTVVIKPAQVTPLTALKFAELTLKAGIPKGVVNVLPGSG SLVGQRLSDHPDVRKIGFTGSTEVGKHIMKSCAISNVKKVSLELGGKSPLIIFADCDLNKAVQMGMSSVF FNKGENCIAAGRLFVEDSIHDEFVRRVVEEVRKMKVGNPLDRDTDHGPQNHHAHLVKLMEYCQHGVKEGA TLVCGGNQVPRPGFFFEPTVFTDVEDHMFIAKEESFGPVMIISRFADGDLDAVLSRANATEFGLASGVFT RDINKALYVSDKLQAGTVFVNTYNKTDVAAPFGGFKQSGFGKDLGEAALNEYLRVKTVTFEY TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_012190 |
| ORF Size | 2706 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_012190.4 |
| RefSeq Size | 3125 bp |
| RefSeq ORF | 2709 bp |
| Locus ID | 10840 |
| UniProt ID | O75891 |
| Domains | aldedh, formyl_transf, formyl_trans_C |
| Protein Families | Druggable Genome |
| Protein Pathways | One carbon pool by folate |
| MW | 98.6 kDa |
| Gene Summary | The protein encoded by this gene catalyzes the conversion of 10-formyltetrahydrofolate, nicotinamide adenine dinucleotide phosphate (NADP+), and water to tetrahydrofolate, NADPH, and carbon dioxide. The encoded protein belongs to the aldehyde dehydrogenase family. Loss of function or expression of this gene is associated with decreased apoptosis, increased cell motility, and cancer progression. There is an antisense transcript that overlaps on the opposite strand with this gene locus. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2012] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC213720L3 | Lenti ORF clone of Human aldehyde dehydrogenase 1 family, member L1 (ALDH1L1), Myc-DDK-tagged |
CNY 9,030.00 |
|
| RC213720L4 | Lenti ORF clone of Human aldehyde dehydrogenase 1 family, member L1 (ALDH1L1), mGFP tagged |
CNY 9,030.00 |
|
| RG213720 | ALDH1L1 (tGFP-tagged) - Human aldehyde dehydrogenase 1 family, member L1 (ALDH1L1) |
CNY 8,208.00 |
|
| SC111756 | ALDH1L1 (untagged)-Human aldehyde dehydrogenase 1 family, member L1 (ALDH1L1) |
CNY 6,616.00 |
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