TRBP (TARBP2) (NM_134323) Human Tagged ORF Clone

CAT#: RC213967

TARBP2 (Myc-DDK-tagged)-Human TAR (HIV-1) RNA binding protein 2 (TARBP2), transcript variant 1

ORF Plasmid: DDK tGFP



  "NM_134323" in other vectors (6)

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CNY 3,656.00

CNY 3,990.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


TARBP2 mouse monoclonal antibody, clone OTI7C1 (formerly 7C1)
    • 100 ul

CNY 1,999.00
CNY 2,700.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms LOQS; TRBP; TRBP1; TRBP2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC213967 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGTGAAGAGGAGCAAGGCTCCGGCACTACCACGGGCTGCGGGCTGCCTAGTATAGAGCAAATGCTGG
CCGCCAACCCAGGCAAGACCCCGATCAGCCTTCTGCAGGAGTATGGGACCAGAATAGGGAAGACGCCTGT
GTACGACCTTCTCAAAGCCGAGGGCCAAGCCCACCAGCCTAATTTCACCTTCCGGGTCACCGTTGGCGAC
ACCAGCTGCACTGGTCAGGGCCCCAGCAAGAAGGCAGCCAAGCACAAGGCAGCTGAGGTGGCCCTCAAAC
ACCTCAAAGGGGGGAGCATGCTGGAGCCGGCCCTGGAGGACAGCAGTTCTTTTTCTCCCCTAGACTCTTC
ACTGCCTGAGGACATTCCGGTTTTTACTGCTGCAGCAGCTGCTACCCCAGTTCCATCTGTAGTCCTAACC
AGGAGCCCCCCCATGGAACTGCAGCCCCCTGTCTCCCCTCAGCAGTCTGAGTGCAACCCCGTTGGTGCTC
TGCAGGAGCTGGTGGTGCAGAAAGGCTGGCGGTTGCCGGAGTACACAGTGACCCAGGAGTCTGGGCCAGC
CCACCGCAAAGAATTCACCATGACCTGTCGAGTGGAGCGTTTCATTGAGATTGGGAGTGGCACTTCCAAA
AAATTGGCAAAGCGGAATGCGGCGGCCAAAATGCTGCTTCGAGTGCACACGGTGCCTCTGGATGCCCGGG
ATGGCAATGAGGTGGAGCCTGATGATGACCACTTCTCCATTGGTGTGGGCTCCCGCCTGGATGGTCTTCG
AAACCGGGGCCCAGGTTGCACCTGGGATTCTCTACGAAATTCAGTAGGAGAGAAGATCCTGTCCCTCCGC
AGTTGCTCCCTGGGCTCCCTGGGTGCCCTGGGCCCTGCCTGCTGCCGTGTCCTCAGTGAGCTCTCTGAGG
AGCAGGCCTTTCACGTCAGCTACCTGGATATTGAGGAGCTGAGCCTGAGTGGACTCTGCCAGTGCCTGGT
GGAACTGTCCACCCAGCCGGCCACTGTGTGTCATGGCTCTGCAACCACCAGGGAGGCAGCCCGTGGTGAG
GCTGCCCGCCGTGCCCTGCAGTACCTCAAGATCATGGCAGGCAGCAAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC213967 protein sequence
Red=Cloning site Green=Tags(s)

MSEEEQGSGTTTGCGLPSIEQMLAANPGKTPISLLQEYGTRIGKTPVYDLLKAEGQAHQPNFTFRVTVGD
TSCTGQGPSKKAAKHKAAEVALKHLKGGSMLEPALEDSSSFSPLDSSLPEDIPVFTAAAAATPVPSVVLT
RSPPMELQPPVSPQQSECNPVGALQELVVQKGWRLPEYTVTQESGPAHRKEFTMTCRVERFIEIGSGTSK
KLAKRNAAAKMLLRVHTVPLDARDGNEVEPDDDHFSIGVGSRLDGLRNRGPGCTWDSLRNSVGEKILSLR
SCSLGSLGALGPACCRVLSELSEEQAFHVSYLDIEELSLSGLCQCLVELSTQPATVCHGSATTREAARGE
AARRALQYLKIMAGSK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_134323
ORF Size 1098 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_134323.2
RefSeq Size 1888 bp
RefSeq ORF 1101 bp
Locus ID 6895
UniProt ID Q15633
Protein Families Stem cell - Pluripotency
MW 39 kDa
Gene Summary HIV-1, the causative agent of acquired immunodeficiency syndrome (AIDS), contains an RNA genome that produces a chromosomally integrated DNA during the replicative cycle. Activation of HIV-1 gene expression by the transactivator Tat is dependent on an RNA regulatory element (TAR) located downstream of the transcription initiation site. The protein encoded by this gene binds between the bulge and the loop of the HIV-1 TAR RNA regulatory element and activates HIV-1 gene expression in synergy with the viral Tat protein. Alternative splicing results in multiple transcript variants encoding different isoforms. This gene also has a pseudogene. [provided by RefSeq, Jul 2008]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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