FMNL1 (NM_005892) Human Tagged ORF Clone

CAT#: RC214008

FMNL1 (Myc-DDK-tagged)-Human formin-like 1 (FMNL1)

ORF Plasmid: DDK tGFP



  "NM_005892" in other vectors (4)

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CNY 11,488.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


Rabbit Polyclonal Anti-FMNL1 Antibody
    • 100 ul

CNY 1,999.00
CNY 3,280.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms C17orf1; C17orf1B; FHOD4; FMNL; KW-13
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC214008 representing NM_005892
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGCAACGCGGCCGGCAGCGCCGAGCAGCCCGCGGGCCCCGCCGCGCCGCCCCCCAAGCAGCCCGCGC
CTCCCAAGCAGCCGATGCCCGCGGCCGGAGAGCTGGAGGAGAGGTTCAACCGCGCCCTGAACTGCATGAA
CTTGCCCCCAGACAAGGTCCAGCTGCTGAGCCAGTATGACAACGAGAAGAAGTGGGAGCTCATCTGTGAT
CAGGAGCGGTTTCAAGTCAAGAATCCCCCCGCAGCCTACATCCAGAAGCTGAAGAGCTATGTGGATACTG
GTGGGGTCAGCCGAAAGGTAGCAGCTGATTGGATGTCCAACCTGGGGTTTAAGAGGCGAGTTCAGGAGTC
CACGCAGGTGCTACGGGAGCTGGAGACCTCCCTGAGGACCAACCACATTGGGTGGGTGCAGGAGTTCCTC
AATGAAGAGAACCGTGGCCTGGATGTGCTGCTCGAGTACCTGGCCTTTGCCCAGTGCTCTGTCACGTATG
ACATGGAGAGCACAGACAACGGGGCTTCCAACTCAGAGAAAAACAAGCCCCTGGAGCAGTCTGTGGAAGA
CCTCAGCAAGGGTCCACCCTCCTCCGTGCCCAAAAGCCGCCACCTGACCATCAAGCTGACCCCAGCCCAC
AGCAGGAAGGCCCTGCGGAATTCCCGCATCGTCAGCCAGAAGGACGACGTCCACGTCTGTATTATGTGCC
TACGCGCCATCATGAACTACCAGTCTGGCTTCAGCCTTGTCATGAACCACCCAGCCTGTGTCAATGAGAT
TGCTCTGAGCCTCAACAACAAGAACCCCAGAACCAAGGCTCTGGTGCTGGAGCTGCTGGCGGCCGTGTGC
TTGGTGCGGGGAGGACATGACATCATCCTTGCAGCCTTTGACAACTTCAAGGAGGTGTGTGGGGAGCAGC
ACCGCTTTGAAAAGCTGATGGAATATTTCCGGAATGAGGACAGCAACATCGACTTCATGGTGGCCTGCAT
GCAGTTCATCAACATTGTGGTACATTCAGTGGAGAACATGAACTTCCGTGTCTTCCTGCAATATGAGTTC
ACCCACTTGGGCCTGGACCTGTACTTGGAGAGGCTTCGGCTCACCGAGAGTGACAAGCTGCAGGTGCAGA
TCCAGGCGTACCTGGACAATATTTTTGATGTGGGGGCGCTGCTGGAGGACACAGAGACCAAGAACGCTGT
GCTGGAGCACATGGAGGAACTGCAGGAGCAAGTGGCGCTGCTGACAGAGCGGCTTCGGGACGCGGAGAAC
GAATCCATGGCCAAGATTGCAGAACTGGAAAAACAGCTAAGCCAGGCGCGCAAGGAGTTGGAGACCCTGC
GGGAGCGCTTCAGCGAATCGACCGCCATGGGCGCCTCCAGGCGTCCCCCAGAGCCTGAGAAAGCGCCTCC
CGCTGCCCCGACGCGGCCCTCGGCCCTGGAGCTGAAGGTGGAGGAGCTGGAGGAGAAGGGGTTAATCCGT
ATTCTGCGGGGGCCGGGGGATGCTGTCTCCATCGAGATCCTCCCCGTCGCTGTGGCAACTCCGAGCGGCG
GTGATGCTCCGACTCCGGGGGTGCCGACCGGCTCCCCCAGCCCAGATCTCGCACCTGCAGCAGAGCCGGC
TCCCGGAGCAGCGCCACCGCCGCCGCCCCCACTGCCCGGCCTCCCCTCCCCGCAGGAAGCCCCGCCCTCT
GCGCCCCCACAGGCCCCGCCTCTCCCTGGCAGCCCGGAGCCCCCGCCTGCGCCGCCGCTGCCCGGAGACC
TGCCGCCCCCACCCCCGCCACCGCCACCACCTCCGGGCACTGACGGGCCGGTGCCTCCGCCGCCGCCGCC
GCCGCCGCCGCCTCCCGGAGGTCCTCCTGATGCCCTAGGAAGACGCGACTCAGAATTGGGCCCAGGAGTG
AAGGCCAAGAAGCCCATCCAGACTAAGTTCCGAATGCCACTCTTGAACTGGGTGGCACTGAAACCCAGCC
AGATCACCGGCACTGTCTTCACAGAGCTCAATGATGAGAAGGTGCTGCAGGAGCTAGACATGAGTGATTT
TGAGGAACAGTTCAAGACCAAGTCCCAAGGCCCCAGCCTGGACCTCAGCGCTCTCAAGAGTAAGGCAGCC
CAGAAGGCCCCCAGCAAGGCGACACTCATTGAGGCCAACCGGGCCAAGAACTTGGCCATCACCCTGCGGA
AGGGCAACCTGGGGGCCGAGCGCATCTGCCAAGCCATTGAGGCGTACGACCTGCAGGCTCTGGGCCTGGA
CTTCCTGGAGCTGCTGATGCGCTTCCTGCCCACAGAGTATGAGCGCAGCCTCATCACCCGCTTTGAGCGG
GAGCAGCGGCCAATGGAGGAGCTGTCAGAGGAGGACCGCTTCATGCTATGCTTCAGCCGCATCCCGCGCC
TGCCGGAGCGCATGACCACACTCACCTTCCTGGGCAACTTCCCGGACACAGCCCAGCTGCTCATGCCGCA
ACTGAATGCCATCATTGCAGCCTCAATGTCCATCAAGTCCTCTGACAAACTCCGCCAGATCCTGGAGATT
GTCCTGGCCTTTGGCAACTACATGAACAGTAGCAAGCGTGGGGCAGCCTATGGCTTCCGGCTCCAGAGCC
TGGATGCGCTGTTGGAGATGAAGTCGACTGATCGCAAGCAGACGCTGCTGCACTACCTGGTGAAGGTCAT
TGCTGAGAAGTACCCGCAACTCACAGGCTTCCACAGCGACCTGCACTTCCTGGACAAGGCGGGCTCAGTG
TCCCTGGACAGTGTCCTGGCGGACGTGCGCTCCCTGCAGCGAGGCCTAGAGTTGACACAGAGAGAGTTTG
TGCGGCAGGATGACTGCATGGTGCTCAAGGAGTTCCTGAGGGCCAACTCGCCCACCATGGACAAGCTGCT
GGCAGACAGCAAGACGGCTCAGGAGGCCTTTGAGTCTGTGGTGGAGTACTTCGGAGAGAACCCCAAGACC
ACATCCCCAGGCCTGTTCTTCTCCCTCTTTAGCCGCTTCATTAAGGCCTACAAGAAAGCTGAGCAGGAGG
TGGAACAGTGGAAAAAAGAAGCCGCTGCCCAGGAGGCAGGCGCTGATACCCCGGGCAAAGGGGAGCCCCC
AGCACCCAAGTCACCGCCAAAGGCCCGGCGGCCACAGATGGACCTCATCTCTGAGCTGAAACGGAGGCAG
CAGAAGGAGCCACTCATTTATGAGAGCGACCGTGATGGGGCCATTGAAGACATCATCACAGTGATCAAGA
CGGTGCCCTTCACGGCCCGCACCGGCAAGCGGACATCCCGGCTCCTCTGTGAGGCCAGCCTGGGAGAAGA
GATGCCCCTC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC214008 representing NM_005892
Red=Cloning site Green=Tags(s)

MGNAAGSAEQPAGPAAPPPKQPAPPKQPMPAAGELEERFNRALNCMNLPPDKVQLLSQYDNEKKWELICD
QERFQVKNPPAAYIQKLKSYVDTGGVSRKVAADWMSNLGFKRRVQESTQVLRELETSLRTNHIGWVQEFL
NEENRGLDVLLEYLAFAQCSVTYDMESTDNGASNSEKNKPLEQSVEDLSKGPPSSVPKSRHLTIKLTPAH
SRKALRNSRIVSQKDDVHVCIMCLRAIMNYQSGFSLVMNHPACVNEIALSLNNKNPRTKALVLELLAAVC
LVRGGHDIILAAFDNFKEVCGEQHRFEKLMEYFRNEDSNIDFMVACMQFINIVVHSVENMNFRVFLQYEF
THLGLDLYLERLRLTESDKLQVQIQAYLDNIFDVGALLEDTETKNAVLEHMEELQEQVALLTERLRDAEN
ESMAKIAELEKQLSQARKELETLRERFSESTAMGASRRPPEPEKAPPAAPTRPSALELKVEELEEKGLIR
ILRGPGDAVSIEILPVAVATPSGGDAPTPGVPTGSPSPDLAPAAEPAPGAAPPPPPPLPGLPSPQEAPPS
APPQAPPLPGSPEPPPAPPLPGDLPPPPPPPPPPPGTDGPVPPPPPPPPPPPGGPPDALGRRDSELGPGV
KAKKPIQTKFRMPLLNWVALKPSQITGTVFTELNDEKVLQELDMSDFEEQFKTKSQGPSLDLSALKSKAA
QKAPSKATLIEANRAKNLAITLRKGNLGAERICQAIEAYDLQALGLDFLELLMRFLPTEYERSLITRFER
EQRPMEELSEEDRFMLCFSRIPRLPERMTTLTFLGNFPDTAQLLMPQLNAIIAASMSIKSSDKLRQILEI
VLAFGNYMNSSKRGAAYGFRLQSLDALLEMKSTDRKQTLLHYLVKVIAEKYPQLTGFHSDLHFLDKAGSV
SLDSVLADVRSLQRGLELTQREFVRQDDCMVLKEFLRANSPTMDKLLADSKTAQEAFESVVEYFGENPKT
TSPGLFFSLFSRFIKAYKKAEQEVEQWKKEAAAQEAGADTPGKGEPPAPKSPPKARRPQMDLISELKRRQ
QKEPLIYESDRDGAIEDIITVIKTVPFTARTGKRTSRLLCEASLGEEMPL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_005892
ORF Size 3300 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_005892.3, NP_005883.2
RefSeq Size 3973 bp
RefSeq ORF 3303 bp
Locus ID 752
UniProt ID O95466
Domains FH2
MW 121.6 kDa
Gene Summary This gene encodes a formin-related protein. Formin-related proteins have been implicated in morphogenesis, cytokinesis, and cell polarity. An alternative splice variant has been described but its full length sequence has not been determined. [provided by RefSeq, Jul 2008]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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