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KIAA0319 (NM_014809) Human Tagged ORF Clone
CAT＃: RC214212
- TrueORF®
KIAA0319 (Myc-DDK-tagged)-Human KIAA0319 (KIAA0319), transcript variant 1
ORF Plasmid: tGFP
"NM_014809" in other vectors (6)
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CNY 7,464.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AAVR; DYLX2; DYX2; NMIG |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC214212 representing NM_014809.
Blue=ORF Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGCGCCCCCCACAGGTGTGCTCTCTTCATTGCTGCTGCTGGTGACAATTGCAGGTTGTGCCCGTAAG CAGTGCAGCGAGGGGAGGACATATTCCAATGCAGTCATTTCACCTAACTTGGAAACCACCAGAATCATG CGGGTGTCTCACACCTTCCCTGTCGTAGACTGCACGGCCGCTTGCTGTGACCTGTCCAGCTGTGACCTG GCCTGGTGGTTCGAGGGCCGCTGCTACCTGGTGAGCTGCCCCCACAAAGAGAACTGTGAGCCCAAGAAG ATGGGCCCCATCAGGTCTTATCTCACTTTTGTGCTCCGGCCTGTTCAGAGGCCTGCACAGCTGCTGGAC TATGGGGACATGATGCTGAACAGGGGCTCCCCCTCGGGGATCTGGGGGGACTCACCTGAGGATATCAGA AAGGACTTGACCTTTCTAGGCAAAGATTGGGGCCTAGAGGAGATGTCTGAGTACTCAGATGACTACCGG GAGCTGGAGAAGGACCTCTTGCAACCCAGTGGCAAGCAGGAGCCCAGAGGGAGTGCCGAGTACACGGAC TGGGGCCTACTGCCGGGCAGCGAGGGGGCCTTCAACTCCTCTGTTGGAGACAGTCCTGCGGTGCCAGCG GAGACGCAGCAGGACCCTGAGCTCCATTACCTGAATGAGTCGGCTTCAACCCCTGCCCCAAAACTCCCT GAGAGAAGTGTGTTGCTTCCCTTGCCGACTACTCCATCTTCAGGAGAGGTGTTGGAGAAAGAAAAGGCT TCTCAGCTCCAGGAACAATCCAGCAACAGCTCTGGAAAAGAGGTTCTAATGCCTTCCCATAGTCTTCCT CCGGCAAGCCTGGAGCTCAGCTCAGTCACCGTGGAGAAAAGCCCAGTGCTCACAGTCACCCCGGGGAGT ACAGAGCACAGCATCCCAACACCTCCCACTAGCGCAGCCCCCTCTGAGTCCACCCCATCTGAGCTACCC ATATCTCCTACCACTGCTCCCAGGACAGTGAAAGAACTTACGGTATCGGCTGGAGATAACCTAATTATA ACTTTACCCGACAATGAAGTTGAACTGAAGGCCTTTGTTGCGCCAGCGCCACCTGTAGAAACAACCTAC AACTATGAATGGAATTTAATAAGCCACCCCACAGACTACCAAGGTGAAATAAAACAAGGACACAAGCAA ACTCTTAACCTCTCTCAATTGTCCGTCGGACTTTATGTCTTCAAAGTCACTGTTTCTAGTGAAAACGCC TTTGGAGAAGGATTTGTCAATGTCACTGTTAAGCCTGCCAGAAGAGTCAACCTGCCACCTGTAGCAGTT GTTTCTCCCCAACTGCAAGAGCTCACTTTGCCTTTGACGTCAGCCCTCATTGATGGCAGCCAAAGTACA GATGATACTGAAATAGTGAGTTATCATTGGGAAGAAATAAACGGGCCCTTCATAGAAGAGAAGACTTCA GTTGACTCTCCCGTCTTACGCTTGTCTAACCTTGATCCTGGTAACTATAGTTTCAGGTTGACTGTTACA GACTCGGACGGAGCCACTAACTCTACAACTGCAGCCCTAATAGTGAACAATGCTGTGGACTACCCACCA GTTGCTAATGCAGGACCAAATCACACCATAACTTTGCCCCAAAACTCCATCACTTTGAATGGAAACCAG AGCAGTGACGATCACCAGATTGTCCTCTATGAGTGGTCCCTGGGTCCTGGGAGTGAGGGCAAACATGTG GTCATGCAGGGAGTACAGACGCCATACCTTCATTTATCTGCAATGCAGGAAGGAGATTATACATTTCAG CTGAAGGTGACAGATTCTTCAAGGCAACAGTCTACTGCTGTGGTGACTGTGATTGTCCAGCCTGAAAAC AATAGACCTCCAGTGGCTGTGGCCGGCCCTGATAAAGAGCTGATCTTCCCAGTGGAAAGTGCTACCCTG GATGGGAGCAGCAGCAGCGATGACCACGGCATTGTCTTCTACCACTGGGAGCACGTCAGAGGCCCCAGT GCAGTGGAGATGGAAAATATTGACAAAGCAATAGCCACTGTGACTGGTCTCCAGGTGGGGACCTACCAC TTCCGTTTGACAGTGAAAGACCAGCAGGGACTGAGCAGCACGTCCACCCTCACTGTGGCTGTGAAGAAG GAAAATAATAGTCCTCCCAGAGCCCGGGCTGGTGGCAGACATGTTCTTGTGCTTCCCAATAATTCCATT ACTTTGGATGGTTCAAGGTCTACTGATGACCAAAGAATTGTGTCCTATCTGTGGATCCGGGATGGCCAG AGTCCAGCAGCTGGAGATGTCATCGATGGCTCTGACCACAGTGTGGCTCTGCAGCTTACGAATCTGGTG GAGGGGGTGTACACTTTCCACTTGCGAGTCACCGACAGTCAGGGGGCCTCGGACACAGACACTGCCACT GTGGAAGTGCAGCCAGACCCTAGGAAGAGTGGCCTGGTGGAGCTGACCCTGCAGGTTGGTGTTGGGCAG CTGACAGAGCAGCGGAAGGACACCCTTGTGAGGCAGCTGGCTGTGCTGCTGAACGTGCTGGACTCGGAC ATTAAGGTCCAGAAGATTCGGGCCCACTCGGATCTCAGCACCGTGATTGTGTTTTATGTACAGAGCAGG CCGCCTTTCAAGGTTCTCAAAGCTGCTGAAGTGGCCCGAAATCTGCACATGCGGCTCTCAAAGGAGAAG GCTGACTTCTTGCTTTTCAAGGTCTTGAGGGTTGATACAGCAGGTTGCCTTCTGAAGTGTTCTGGCCAT GGTCACTGCGACCCCCTCACAAAGCGCTGCATTTGCTCTCACTTATGGATGGAGAACCTTATACAGCGT TATATCTGGGATGGAGAGAGCAACTGTGAGTGGAGTATATTCTATGTGACAGTGTTGGCTTTTACTCTT ATTGTGCTAACAGGAGGTTTCACTTGGCTTTGCATCTGCTGCTGCAAAAGACAAAAAAGGACTAAAATC AGGAAAAAAACAAAGTACACCATCCTGGATAACATGGATGAACAGGAAAGAATGGAACTGAGGCCCAAA TATGGTATCAAGCACCGAAGCACAGAGCACAACTCCAGCCTGATGGTATCCGAGTCTGAGTTTGACAGT GACCAGGACACAATCTTCAGCCGAGAAAAGATGGAGAGAGGGAATCCAAAGGTTTCCATGAATGGTTCC ATCAGAAATGGAGCTTCCTTCAGTTATTGCTCAAAGGACAGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC >Peptide sequence encoded by RC214212
Blue=ORF Red=Cloning site Green=Tag(s) MAPPTGVLSSLLLLVTIAGCARKQCSEGRTYSNAVISPNLETTRIMRVSHTFPVVDCTAACCDLSSCDL AWWFEGRCYLVSCPHKENCEPKKMGPIRSYLTFVLRPVQRPAQLLDYGDMMLNRGSPSGIWGDSPEDIR KDLTFLGKDWGLEEMSEYSDDYRELEKDLLQPSGKQEPRGSAEYTDWGLLPGSEGAFNSSVGDSPAVPA ETQQDPELHYLNESASTPAPKLPERSVLLPLPTTPSSGEVLEKEKASQLQEQSSNSSGKEVLMPSHSLP PASLELSSVTVEKSPVLTVTPGSTEHSIPTPPTSAAPSESTPSELPISPTTAPRTVKELTVSAGDNLII TLPDNEVELKAFVAPAPPVETTYNYEWNLISHPTDYQGEIKQGHKQTLNLSQLSVGLYVFKVTVSSENA FGEGFVNVTVKPARRVNLPPVAVVSPQLQELTLPLTSALIDGSQSTDDTEIVSYHWEEINGPFIEEKTS VDSPVLRLSNLDPGNYSFRLTVTDSDGATNSTTAALIVNNAVDYPPVANAGPNHTITLPQNSITLNGNQ SSDDHQIVLYEWSLGPGSEGKHVVMQGVQTPYLHLSAMQEGDYTFQLKVTDSSRQQSTAVVTVIVQPEN NRPPVAVAGPDKELIFPVESATLDGSSSSDDHGIVFYHWEHVRGPSAVEMENIDKAIATVTGLQVGTYH FRLTVKDQQGLSSTSTLTVAVKKENNSPPRARAGGRHVLVLPNNSITLDGSRSTDDQRIVSYLWIRDGQ SPAAGDVIDGSDHSVALQLTNLVEGVYTFHLRVTDSQGASDTDTATVEVQPDPRKSGLVELTLQVGVGQ LTEQRKDTLVRQLAVLLNVLDSDIKVQKIRAHSDLSTVIVFYVQSRPPFKVLKAAEVARNLHMRLSKEK ADFLLFKVLRVDTAGCLLKCSGHGHCDPLTKRCICSHLWMENLIQRYIWDGESNCEWSIFYVTVLAFTL IVLTGGFTWLCICCCKRQKRTKIRKKTKYTILDNMDEQERMELRPKYGIKHRSTEHNSSLMVSESEFDS DQDTIFSREKMERGNPKVSMNGSIRNGASFSYCSKDR TRTRPLEQKLISEEDLAANDILDYKDDDDKV Recombinant protein using RC214212 also available, TP314212 |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_014809 |
| ORF Size | 3216 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_014809.4 |
| RefSeq Size | 6801 bp |
| RefSeq ORF | 3219 bp |
| Locus ID | 9856 |
| UniProt ID | Q5VV43 |
| Domains | PKD |
| Protein Families | Transmembrane |
| MW | 117.8 kDa |
| Gene Summary | This gene encodes a transmembrane protein that contains a large extracellular domain with multiple polycystic kidney disease (PKD) domains. The encoded protein may play a role in the development of the cerebral cortex by regulating neuronal migration and cell adhesion. Single nucleotide polymorphisms in this gene are associated with dyslexia. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2011] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC214212L1 | Lenti ORF clone of Human KIAA0319 (KIAA0319), transcript variant 1, Myc-DDK-tagged |
CNY 9,864.00 |
|
| RC214212L2 | Lenti ORF clone of Human KIAA0319 (KIAA0319), transcript variant 1, mGFP tagged |
CNY 12,640.00 |
|
| RC214212L3 | Lenti ORF clone of Human KIAA0319 (KIAA0319), transcript variant 1, Myc-DDK-tagged |
CNY 12,640.00 |
|
| RC214212L4 | Lenti ORF clone of Human KIAA0319 (KIAA0319), transcript variant 1, mGFP tagged |
CNY 12,640.00 |
|
| RG214212 | KIAA0319 (tGFP-tagged) - Human KIAA0319 (KIAA0319), transcript variant 1 |
CNY 9,064.00 |
|
| SC315140 | KIAA0319 (untagged)-Human KIAA0319 (KIAA0319), transcript variant 1 |
CNY 10,450.00 |
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