SLC22A17 (NM_016609) Human Tagged ORF Clone

CAT#: RC214220

SLC22A17 (Myc-DDK-tagged)-Human solute carrier family 22, member 17 (SLC22A17), transcript variant 2

ORF Plasmid: DDK tGFP



  "NM_016609" in other vectors (6)

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CNY 3,872.00

CNY 4,560.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


SLC22A17 mouse monoclonal antibody,clone OTI2D6
    • 100 ul

CNY 1,999.00
CNY 2,700.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms 24p3R; BOCT; BOIT; hBOIT; NGALR; NGALR2; NGALR3
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC214220 representing NM_016609
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCTCGGACCCCATCTTCACGCTGGCGCCCCCGCTGCATTGCCACTACGGGGCCTTCCCCCCTAATG
CCTCTGGCTGGGAGCAGCCTCCCAATGCCAGCGGCGTCAGCGTCGCCAGCGCTGCCCTAGCAGCCAGCGC
CGCCAGCCGTGTCGCCACCAGTACCGACCCCTCGTGCAGCGGCTTCGCCCCGCCGGACTTCAACCATTGC
CTCAAGGATTGGGACTATAATGGCCTTCCTGTGCTCACCACCAACGCCATCGGCCAGTGGGATCTGGTGT
GTGACCTGGGCTGGCAGGTGATCCTGGAGCAGATCCTCTTCATCTTGGGCTTTGCCTCCGGCTACCTGTT
CCTGGGTTACCCCGCAGACAGATTTGGCCGTCGCGGGATTGTGCTGCTGACCTTGGGGCTGGTGGGCCCC
TGTGGAGTAGGAGGGGCTGCTGCAGGCTCCTCCACAGGCGTCATGGCCCTCCGATTCCTCTTGGGCTTTC
TGCTTGCCGGTGTTGACCTGGGTGTCTACCTGATGCGCCTGGAGCTGTGCGACCCAACCCAGAGGCTTCG
GGTGGCCCTGGCAGGGGAGTTGGTGGGGGTGGGAGGGCACTTCCTGTTCCTGGGCCTGGCCCTTGTCTCT
AAGGATTGGCGATTCCTACAGCGAATGATCACCGCTCCCTGCATCCTCTTCCTGTTTTATGGCTGGCCTG
GTTTGTTCCTGGAGTCCGCACGGTGGCTGATAGTGAAGCGGCAGATTGAGGAGGCTCAGTCTGTGCTGAG
GATCCTGGCTGAGCGAAACCGGCCCCATGGGCAGATGCTGGGGGAGGAGGCCCAGGAGGCCCTGCAGGAC
CTGGAGAATACCTGCCCTCTCCCTGCAACATCCTCCTTTTCCTTTGCTTCCCTCCTCAACTACCGCAACA
TCTGGAAAAATCTGCTTATCCTGGGCTTCACCAACTTCATTGCCCATGCCATTCGCCACTGCTACCAGCC
TGTGGGAGGAGGAGGGAGCCCATCGGACTTCTACCTGTGCTCTCTGCTGGCCAGCGGCACCGCAGCCCTG
GCCTGTGTCTTCCTGGGGGTCACCGTGGACCGATTTGGCCGCCGGGGCATCCTTCTTCTCTCCATGACCC
TTACCGGCATTGCTTCCCTGGTCCTGCTGGGCCTGTGGGATTATCTGAACGAGGCTGCCATCACCACTTT
CTCTGTCCTTGGGCTCTTCTCCTCCCAAGCTGCCGCCATCCTCAGCACCCTCCTTGCTGCTGAGGTCATC
CCCACCACTGTCCGGGGCCGTGGCCTGGGCCTGATCATGGCTCTAGGGGCGCTTGGAGGACTGAGCGGCC
CGGCCCAGCGCCTCCACATGGGCCATGGAGCCTTCCTGCAGCACGTGGTGCTGGCGGCCTGCGCCCTCCT
CTGCATTCTCAGCATTATGCTGCTGCCGGAGACCAAGCGCAAGCTCCTGCCCGAGGTGCTCCGGGACGGG
GAGCTGTGTCGCCGGCCTTCCCTGCTGCGGCAGCCACCCCCTACCCGCTGTGACCACGTCCCGCTGCTTG
CCACCCCCAACCCTGCCCTC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC214220 representing NM_016609
Red=Cloning site Green=Tags(s)

MASDPIFTLAPPLHCHYGAFPPNASGWEQPPNASGVSVASAALAASAASRVATSTDPSCSGFAPPDFNHC
LKDWDYNGLPVLTTNAIGQWDLVCDLGWQVILEQILFILGFASGYLFLGYPADRFGRRGIVLLTLGLVGP
CGVGGAAAGSSTGVMALRFLLGFLLAGVDLGVYLMRLELCDPTQRLRVALAGELVGVGGHFLFLGLALVS
KDWRFLQRMITAPCILFLFYGWPGLFLESARWLIVKRQIEEAQSVLRILAERNRPHGQMLGEEAQEALQD
LENTCPLPATSSFSFASLLNYRNIWKNLLILGFTNFIAHAIRHCYQPVGGGGSPSDFYLCSLLASGTAAL
ACVFLGVTVDRFGRRGILLLSMTLTGIASLVLLGLWDYLNEAAITTFSVLGLFSSQAAAILSTLLAAEVI
PTTVRGRGLGLIMALGALGGLSGPAQRLHMGHGAFLQHVVLAACALLCILSIMLLPETKRKLLPEVLRDG
ELCRRPSLLRQPPPTRCDHVPLLATPNPAL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_016609
ORF Size 1560 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_016609.4, NP_057693.3
RefSeq Size 2344 bp
RefSeq ORF 1563 bp
Locus ID 51310
UniProt ID Q8WUG5
Protein Families Druggable Genome, Transmembrane
MW 55.5 kDa
Gene Summary Cell surface receptor for LCN2 (24p3) that plays a key role in iron homeostasis and transport. Able to bind iron-bound LCN2 (holo-24p3), followed by internalization of holo-24p3 and release of iron, thereby increasing intracellular iron concentration and leading to inhibition of apoptosis. Also binds iron-free LCN2 (apo-24p3), followed by internalization of apo-24p3 and its association with an intracellular siderophore, leading to iron chelation and iron transfer to the extracellular medium, thereby reducing intracellular iron concentration and resulting in apoptosis (By similarity).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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