Acrosin (ACR) (NM_001097) Human Tagged ORF Clone
CAT#: RC214256
- TrueORF®
ACR (Myc-DDK-tagged)-Human acrosin (ACR)
ORF Plasmid: tGFP
"NM_001097" in other vectors (6)
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CNY 3,656.00
CNY 3,990.00
Cited in 1 publication. |
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC214256 representing NM_001097
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTTGAGATGCTACCAACTGCCATTCTGCTGGTCTTGGCAGTGTCCGTGGTTGCTAAAGATAACGCCA CGTGTGATGGCCCCTGTGGGTTACGGTTCAGGCAAAACCCACAGGGTGGTGTCCGCATCGTCGGCGGGAA GGCTGCACAGCATGGGGCCTGGCCCTGGATGGTCAGCCTCCAGATCTTCACGTACAACAGCCACAGGTAC CACACATGTGGAGGCAGCTTGCTGAATTCACGATGGGTGCTCACTGCTGCTCACTGCTTCGTCGGCAAAA ATAATGTGCATGACTGGAGACTGGTTTTCGGAGCAAAGGAAATTACATATGGGAACAATAAACCAGTAAA GGCGCCTGTGCAAGAGAGATATGTGGAGAAAATCATCATTCATGAAAAATACAACTCTGCGACAGAGGGA AATGACATTGCCCTCGTGGAGATCACCCCTCCCATTTCGTGTGGGCGCTTCATTGGGCCGGGCTGCCTGC CCCACTTGAAGGCAGGCCTCCCCAGAGGCTCCCAGAGCTGCTGGGTGGCCGGCTGGGGATATATAGAAGA GAAAGCCCCCAGGCCATCATCTATACTGATGGAGGCACGTGTGGATCTCATCGACCTGGACTTGTGTAAC TCGACCCAGTGGTACAATGGGCGCGTTCAGCCAACCAATGTGTGCGCGGGGTATCCTGTAGGCAAGATCG ACACCTGCCAGGGAGACAGCGGCGGGCCTCTCATGTGCAAAGACAGCAAGGAAAGCGCCTATGTGGTCGT GGGAATCACAAGCTGGGGGGTAGGCTGTGCCCGTGCCAAGCGCCCCGGAATCTACACGGCCACCTGGCCT TATCTGAACTGGATCGCCTCCAAGATTGGTTCTAACGCTTTGCGTATGATTCAATCGGCCACCCCTCCAC CGCCCACCACTCGACCGCCCCCGATTCGACCCCCCTTCTCCCACCCTATCTCTGCTCACCTTCCTTGGTA TTTCCAACCGCCCCCTCGACCACTTCCACCCCGACCACCGGCAGCCCAGCCCCGACCCCCACCTTCACCC CCGCCCCCACCCCCACCTCCAGCCTCACCTTTACCCCCACCCCCACCCCCACCCCCACCTACACCCTCAT CTACCACAAAACTTCCCCAAGGACTTTCTTTTGCCAAGCGCCTACAGCAGCTCATAGAGGTCTTGAAGGG GAAGACCTATTCCGACGGAAAGAACCATTATGACATGGAGACCACAGAGCTCCCAGAACTGACCTCGACC TCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC214256 representing NM_001097
Red=Cloning site Green=Tags(s) MVEMLPTAILLVLAVSVVAKDNATCDGPCGLRFRQNPQGGVRIVGGKAAQHGAWPWMVSLQIFTYNSHRY HTCGGSLLNSRWVLTAAHCFVGKNNVHDWRLVFGAKEITYGNNKPVKAPVQERYVEKIIIHEKYNSATEG NDIALVEITPPISCGRFIGPGCLPHLKAGLPRGSQSCWVAGWGYIEEKAPRPSSILMEARVDLIDLDLCN STQWYNGRVQPTNVCAGYPVGKIDTCQGDSGGPLMCKDSKESAYVVVGITSWGVGCARAKRPGIYTATWP YLNWIASKIGSNALRMIQSATPPPPTTRPPPIRPPFSHPISAHLPWYFQPPPRPLPPRPPAAQPRPPPSP PPPPPPPASPLPPPPPPPPPTPSSTTKLPQGLSFAKRLQQLIEVLKGKTYSDGKNHYDMETTELPELTST S TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_001097 |
ORF Size | 1263 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001097.1, NP_001088.1 |
RefSeq Size | 1388 bp |
RefSeq ORF | 1266 bp |
Locus ID | 49 |
UniProt ID | P10323 |
Protein Families | Druggable Genome, Protease |
MW | 45.8 kDa |
Gene Summary | Acrosin is the major proteinase present in the acrosome of mature spermatozoa. It is a typical serine proteinase with trypsin-like specificity. It is stored in the acrosome in its precursor form, proacrosin. The active enzyme functions in the lysis of the zona pellucida, thus facilitating penetration of the sperm through the innermost glycoprotein layers of the ovum. The mRNA for proacrosin is synthesized only in the postmeiotic stages of spermatogenesis. In humans proacrosin first appears in the haploid spermatids. [provided by RefSeq, Jul 2008] |
Citations (1)
The use of this cDNA Clones has been cited in the following citations: |
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SPINK2 deficiency causes infertility by inducing sperm defects in heterozygotes and azoospermia in homozygotes
,null,
EMBO Molecular Medicine
,PubMed ID 28554943
[ACR]
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Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC214256L1 | Lenti ORF clone of Human acrosin (ACR), Myc-DDK-tagged |
CNY 6,056.00 |
|
RC214256L2 | Lenti ORF clone of Human acrosin (ACR), mGFP tagged |
CNY 5,890.00 |
|
RC214256L3 | Lenti ORF clone of Human acrosin (ACR), Myc-DDK-tagged |
CNY 5,890.00 |
|
RC214256L4 | Lenti ORF clone of Human acrosin (ACR), mGFP tagged |
CNY 5,890.00 |
|
RG214256 | ACR (tGFP-tagged) - Human acrosin (ACR) |
CNY 4,370.00 |
|
SC310411 | ACR (untagged)-Human acrosin (ACR) |
CNY 6,750.00 |
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