SCN7A (NM_002976) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	NaG; Nav2.1; Nav2.2; SCN6A
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC214432 representing NM_002976 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTGGCTTCACCAGAACCTAAGGGCCTTGTTCCCTTCACTAAAGAGTCTTTTGAACTTATAAAACAGC ATATTGCTAAAACACATAATGAAGACCATGAAGAAGAAGACTTAAAGCCAACTCCTGATTTGGAAGTTGG CAAAAAGCTTCCATTTATTTATGGAAACCTTTCTCAAGGAATGGTGTCAGAGCCCTTGGAAGATGTGGAC CCATATTACTACAAGAAAAAAAATACTTTCATAGTATTAAATAAAAATAGAACAATCTTCAGATTCAATG CGGCTTCCATCTTGTGTACATTGTCTCCTTTCAATTGTATTAGAAGAACAACTATCAAGGTTTTGGTACA TCCCTTTTTCCAACTGTTTATTCTAATTAGTGTCCTGATTGATTGCGTATTCATGTCCCTGACTAATTTG CCAAAATGGAGACCAGTATTAGAGAATACTTTGCTTGGAATTTACACATTTGAAATACTTGTAAAACTCT TTGCAAGAGGTGTCTGGGCAGGATCATTTTCCTTCCTCGGTGATCCATGGAACTGGCTCGATTTCAGCGT AACTGTGTTTGAGGTTATTATAAGATACTCACCTCTGGACTTCATTCCAACGCTTCAAACTGCAAGAACT TTGAGAATTTTAAAAATTATTCCTTTAAATCAAGGTCTGAAATCCCTTGTAGGGGTCCTGATCCACTGCT TGAAGCAGCTTATTGGTGTCATTATCCTAACTCTGTTTTTTCTGAGCATATTTTCTCTAATTGGGATGGG GCTCTTCATGGGCAACTTGAAACATAAATGTTTTCGATGGCCCCAAGAGAATGAAAATGAAACCCTGCAC AACAGAACTGGAAACCCATATTATATTCGAGAAACAGAAAACTTTTATTATTTGGAAGGAGAAAGATATG CTCTCCTTTGTGGCAACAGGACAGATGCTGGTCAGTGTCCTGAAGGATATGTGTGTGTAAAAGCTGGCAT AAATCCTGATCAAGGCTTCACAAATTTTGACAGTTTTGGCTGGGCCTTATTTGCCCTATTTCGGTTAATG GCTCAGGATTACCCTGAAGTACTTTATCACCAGATACTTTATGCTTCTGGGAAGGTCTACATGATATTTT TTGTGGTGGTAAGTTTTTTGTTTTCCTTTTATATGGCAAGTTTGTTCTTAGGCATACTTGCCATGGCCTA TGAAGAAGAAAAGCAGAGAGTTGGTGAAATATCTAAGAAGATTGAACCAAAATTTCAACAGACTGGAAAA GAACTTCAAGAAGGAAATGAAACAGATGAGGCCAAGACCATACAAATAGAAATGAAGAAAAGGTCACCAA TTTCCACAGACACATCATTGGATGTGTTGGAAGATGCTACTCTCAGACATAAGGAAGAACTTGAAAAATC CAAGAAGATATGCCCATTATACTGGTATAAGTTTGCTAAAACTTTCTTGATCTGGAATTGTTCTCCCTGT TGGTTAAAATTGAAAGAGTTTGTCCATAGGATTATAATGGCACCATTTACTGATCTTTTCCTTATCATAT GCATAATTTTAAACGTATGTTTTCTGACCTTGGAGCATTATCCAATGAGTAAACAAACTAACACTCTTCT CAACATTGGAAACCTGGTTTTCATTGGAATTTTCACAGCAGAAATGATTTTTAAAATAATTGCAATGCAT CCATATGGGTATTTCCAAGTAGGTTGGAACATTTTTGATAGCATGATAGTGTTCCATGGTTTAATAGAAC TTTGTCTAGCAAATGTTGCAGGAATGGCTCTTCTTCGATTATTCAGGATGTTAAGAATTTTCAAGTTGGG AAAGTATTGGCCAACATTCCAGATTTTGATGTGGTCTCTTAGTAACTCATGGGTGGCCCTGAAAGACTTG GTCCTGTTGTTGTTCACATTCATCTTCTTTTCTGCTGCATTCGGCATGAAGCTGTTTGGTAAGAATTATG AAGAATTTGTCTGCCACATAGACAAAGACTGTCAACTCCCACGCTGGCACATGCATGACTTTTTCCACTC CTTCCTGAATGTGTTCCGAATTCTCTGTGGAGAGTGGGTAGAGACCTTGTGGGACTGTATGGAGGTTGCA GGCCAATCCTGGTGTATTCCTTTTTACCTGATGGTCATTTTAATTGGAAATTTACTGGTACTTTACCTGT TTCTGGCATTGGTGAGCTCATTTAGTTCATGCAAGGATGTAACAGCTGAAGAGAATAATGAAGCAAAAAA TCTCCAGCTTGCAGTGGCAAGAATTAAAAAAGGAATAAACTATGTGCTTCTTAAAATACTATGCAAAACA CAAAATGTCCCAAAGGACACAATGGACCATGTAAATGAGGTATATGTTAAAGAAGATATTTCTGACCATA CCCTTTCTGAATTGAGCAACACCCAAGATTTTCTCAAAGATAAGGAAAAAAGCAGTGGCACAGAGAAAAA CGCTACTGAAAATGAGAGCCAATCACTTATCCCCAGTCCTAGTGTCTCAGAAACTGTACCAATTGCTTCA GGAGAATCTGATATAGAAAATCTGGATAATAAGGAGATTCAGAGTAAGTCTGGTGATGGAGGCAGCAAAG AGAAAATAAAGCAATCTAGCTCATCTGAATGCAGTACTGTTGATATTGCTATCTCTGAAGAAGAAGAAAT GTTCTATGGAGGTGAAAGATCAAAGCATCTGAAAAATGGTTGCAGACGCGGATCTTCACTTGGTCAAATC AGTGGAGCATCCAAGAAAGGAAAAATCTGGCAGAACATCAGGAAAACCTGCTGCAAGATTGTAGAGAACA ATTGGTTTAAGTGTTTTATTGGGCTTGTTACTCTGCTCAGCACTGGCACTCTGGCTTTTGAAGATATATA TATGGATCAGAGAAAGACAATTAAAATTTTATTAGAATATGCTGACATGATCTTTACTTATATCTTCATT CTGGAAATGCTTCTAAAATGGATGGCATATGGTTTTAAGGCCTATTTCTCTAATGGCTGGTACAGGCTGG ACTTCGTGGTTGTTATTGTGTTTTGTCTTAGCTTAATAGGCAAAACTCGGGAAGAACTAAAACCTCTTAT TTCCATGAAATTCCTTCGGCCCCTCAGAGTTCTATCTCAATTTGAAAGAATGAAGGTGGTTGTGAGAGCT TTGATCAAAACAACCTTACCCACTTTGAATGTGTTTCTTGTCTGCCTGATGATCTGGCTGATTTTTAGTA TCATGGGAGTAGACTTATTTGCTGGCAGATTCTATGAATGCATTGACCCAACAAGTGGAGAAAGGTTTCC TTCATCTGAAGTCATGAATAAGAGTCGGTGTGAAAGCCTTCTGTTTAACGAATCCATGCTATGGGAAAAT GCAAAAATGAACTTTGATAATGTTGGAAATGGTTTCCTTTCTCTGCTTCAAGTAGCAACATTTAATGGAT GGATCACTATTATGAATTCAGCAATTGATTCTGTTGCTGTTAATATACAGCCTCATTTTGAAGTCAACAT CTACATGTATTGTTACTTTATCAACTTTATTATATTTGGAGTATTTCTCCCTCTGAGTATGCTGATTACT GTTATTATTGATAATTTCAACAAGCATAAAATAAAGCTGGGAGGCTCAAATATCTTTATAACGGTTAAAC AGAGAAAACAGTACCGCAGGCTGAAGAAGCTAATGTATGAGGATTCTCAAAGACCAGTACCTCGCCCATT AAACAAGCTCCAAGGATTCATCTTTGATGTGGTAACAAGCCAAGCTTTTAATGTCATTGTTATGGTTCTT ATATGTTTCCAAGCAATAGCCATGATGATAGACACTGATGTTCAGAGTCTACAAATGTCCATTGCTCTCT ACTGGATTAACTCAATTTTTGTTATGCTATATACTATGGAATGTATACTGAAGCTCATCGCTTTCCGTTG TTTTTATTTCACCATTGCGTGGAACATTTTTGATTTTATGGTGGTTATTTTCTCCATCACAGGACTATGT CTGCCTATGACAGTAGGATCCTACCTTGTGCCTCCTTCACTTGTGCAACTGATACTTCTCTCACGGATCA TTCACATGCTGCGTCTTGGAAAAGGACCAAAGGTGTTTCATAATCTGATGCTTCCTTTGATGCTGTCCCT CCCAGCATTATTGAACATCATTCTTCTCATCTTCCTGGTCATGTTCATCTATGCCGTATTTGGAATGTAT AATTTTGCCTATGTTAAAAAAGAAGCTGGAATTAATGATGTGTCTAATTTTGAAACCTTTGGCAACAGTA TGCTCTGTCTTTTTCAAGTTGCAATATTTGCTGGTTGGGATGGGATGCTTGATGCAATTTTCAACAGTAA ATGGTCTGACTGTGATCCTGATAAAATTAACCCTGGGACTCAAGTTAGAGGAGATTGTGGGAACCCCTCT GTTGGGATTTTTTATTTTGTCAGTTATATCCTCATATCATGGCTGATCATTGTAAATATGTACATTGTTG TTGTCATGGAGTTTTTAAATATTGCTTCTAAGAAGAAAAACAAGACCTTGAGTGAAGATGATTTTAGGAA ATTCTTTCAGGTATGGAAAAGGTTTGATCCTGATAGGACCCAGTACATAGACTCTAGCAAGCTTTCAGAT TTTGCAGCTGCTCTTGATCCTCCTCTTTTCATGGCAAAACCAAACAAGGGCCAGCTCATTGCTTTGGACC TCCCCATGGCTGTTGGGGACAGAATTCATTGCCTCGATATCTTACTTGCTTTTACAAAGAGAGTTATGGG TCAAGATGTGAGGATGGAGAAAGTTGTTTCAGAAATAGAATCAGGGTTTTTGTTAGCCAACCCTTTTAAG ATCACATGTGAGCCAATTACGACTACTTTGAAACGAAAACAAGAGGCAGTTTCAGCAACCATCATTCAAC GTGCTTATAAAAATTACCGCTTGAGGCGAAATGACAAAAATACATCAGATATTCATATGATAGATGGTGA CAGAGATGTTCATGCTACTAAAGAAGGTGCCTATTTTGACAAAGCTAAGGAAAAGTCACCTATTCAAAGC CAGATC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC214432 representing NM_002976 Red=Cloning site Green=Tags(s) MLASPEPKGLVPFTKESFELIKQHIAKTHNEDHEEEDLKPTPDLEVGKKLPFIYGNLSQGMVSEPLEDVD PYYYKKKNTFIVLNKNRTIFRFNAASILCTLSPFNCIRRTTIKVLVHPFFQLFILISVLIDCVFMSLTNL PKWRPVLENTLLGIYTFEILVKLFARGVWAGSFSFLGDPWNWLDFSVTVFEVIIRYSPLDFIPTLQTART LRILKIIPLNQGLKSLVGVLIHCLKQLIGVIILTLFFLSIFSLIGMGLFMGNLKHKCFRWPQENENETLH NRTGNPYYIRETENFYYLEGERYALLCGNRTDAGQCPEGYVCVKAGINPDQGFTNFDSFGWALFALFRLM AQDYPEVLYHQILYASGKVYMIFFVVVSFLFSFYMASLFLGILAMAYEEEKQRVGEISKKIEPKFQQTGK ELQEGNETDEAKTIQIEMKKRSPISTDTSLDVLEDATLRHKEELEKSKKICPLYWYKFAKTFLIWNCSPC WLKLKEFVHRIIMAPFTDLFLIICIILNVCFLTLEHYPMSKQTNTLLNIGNLVFIGIFTAEMIFKIIAMH PYGYFQVGWNIFDSMIVFHGLIELCLANVAGMALLRLFRMLRIFKLGKYWPTFQILMWSLSNSWVALKDL VLLLFTFIFFSAAFGMKLFGKNYEEFVCHIDKDCQLPRWHMHDFFHSFLNVFRILCGEWVETLWDCMEVA GQSWCIPFYLMVILIGNLLVLYLFLALVSSFSSCKDVTAEENNEAKNLQLAVARIKKGINYVLLKILCKT QNVPKDTMDHVNEVYVKEDISDHTLSELSNTQDFLKDKEKSSGTEKNATENESQSLIPSPSVSETVPIAS GESDIENLDNKEIQSKSGDGGSKEKIKQSSSSECSTVDIAISEEEEMFYGGERSKHLKNGCRRGSSLGQI SGASKKGKIWQNIRKTCCKIVENNWFKCFIGLVTLLSTGTLAFEDIYMDQRKTIKILLEYADMIFTYIFI LEMLLKWMAYGFKAYFSNGWYRLDFVVVIVFCLSLIGKTREELKPLISMKFLRPLRVLSQFERMKVVVRA LIKTTLPTLNVFLVCLMIWLIFSIMGVDLFAGRFYECIDPTSGERFPSSEVMNKSRCESLLFNESMLWEN AKMNFDNVGNGFLSLLQVATFNGWITIMNSAIDSVAVNIQPHFEVNIYMYCYFINFIIFGVFLPLSMLIT VIIDNFNKHKIKLGGSNIFITVKQRKQYRRLKKLMYEDSQRPVPRPLNKLQGFIFDVVTSQAFNVIVMVL ICFQAIAMMIDTDVQSLQMSIALYWINSIFVMLYTMECILKLIAFRCFYFTIAWNIFDFMVVIFSITGLC LPMTVGSYLVPPSLVQLILLSRIIHMLRLGKGPKVFHNLMLPLMLSLPALLNIILLIFLVMFIYAVFGMY NFAYVKKEAGINDVSNFETFGNSMLCLFQVAIFAGWDGMLDAIFNSKWSDCDPDKINPGTQVRGDCGNPS VGIFYFVSYILISWLIIVNMYIVVVMEFLNIASKKKNKTLSEDDFRKFFQVWKRFDPDRTQYIDSSKLSD FAAALDPPLFMAKPNKGQLIALDLPMAVGDRIHCLDILLAFTKRVMGQDVRMEKVVSEIESGFLLANPFK ITCEPITTTLKRKQEAVSATIIQRAYKNYRLRRNDKNTSDIHMIDGDRDVHATKEGAYFDKAKEKSPIQS QI TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_002976
ORF Size	5046 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_002976.4
RefSeq Size	7186 bp
RefSeq ORF	5049 bp
Locus ID	6332
UniProt ID	Q01118
Protein Families	Druggable Genome, Ion Channels: Other, Transmembrane
MW	193.5 kDa
Gene Summary	This gene encodes one of the many voltage-gated sodium channel proteins. For proper functioning of neurons and muscles during action potentials, voltage-gated sodium channels direct sodium ion diffusion for membrane depolarization. This sodium channel protein has some atypical characteristics; the similarity between the human and mouse proteins is lower compared to other orthologous sodium channel pairs. Also, the S4 segments, which sense voltage changes, have fewer positive charged residues that in other sodium channels; domain 4 has fewer arginine and lysine residues compared to other sodium channel proteins. Several alternatively spliced transcript variants exist, but the full-length natures of all of them remain unknown. [provided by RefSeq, Dec 2011]

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