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AKR7A2 (NM_003689) Human Tagged ORF Clone
CAT＃: RC214673
AKR7A2 (Myc-DDK-tagged)-Human aldo-keto reductase family 7, member A2 (aflatoxin aldehyde reductase) (AKR7A2)
ORF Plasmid: tGFP
"NM_003689" in other vectors (4)
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CNY 3,656.00
CNY 3,990.00
Product images
CNY 300.00
CNY 6,281.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AFAR; AFAR1; AFB1-AR1; AKR7 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC214673 representing NM_003689
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGAGTGCCGCGTCTCGCGTAGTCTCCCGCGCCGCCGTCCACTGCGCGCTTCGCTCTCCGCCGCCCG AGGCCCGCGCGCTCGCCATGTCCCGGCCACCGCCACCGCGGGTCGCCTCGGTGCTGGGCACCATGGAGAT GGGGCGCCGCATGGACGCGCCCGCCAGCGCCGCGGCCGTGCGCGCCTTTCTGGAGCGCGGCCACACCGAA CTGGACACGGCCTTCATGTACAGCGACGGCCAGTCCGAGACCATCCTGGGCGGCCTGGGGCTCGGGCTGG GCGGTGGCGACTGCAGAGTGAAAATTGCCACCAAGGCCAACCCTTGGGATGGAAAATCACTAAAGCCTGA CAGTGTCCGGTCCCAGCTGGAGACGTCATTGAAGAGGCTGCAGTGTCCCCAAGTGGACCTCTTCTACCTA CACGCACCTGACCACGGCACCCCGGTGGAAGAGACGCTGCATGCCTGCCAGCGGCTGCACCAGGAGGGCA AGTTCGTGGAGCTTGGCCTCTCCAACTATGCTAGCTGGGAAGTGGCCGAGATCTGTACCCTCTGCAAGAG CAATGGCTGGATCCTGCCCACTGTGTACCAGGGCATGTACAACGCCACCACCCGGCAGGTGGAAACGGAG CTCTTCCCCTGCCTCAGGCACTTTGGACTGAGGTTCTATGCCTACAACCCTCTGGCTGGGGGCCTGCTGA CTGGCAAGTACAAGTATGAGGACAAGGACGGGAAACAGCCTGTGGGCCGCTTCTTTGGGAATAGCTGGGC TGAGACCTACAGGAATCGCTTCTGGAAGGAGCACCACTTCGAGGCCATTGCGTTGGTGGAGAAGGCCCTG CAGGCCGCATATGGCGCCAGCGCCCCCAGTGTGACCTCGGCTGCCCTCCGGTGGATGTACCACCACTCAC AGCTGCAGGGTGCCCACGGGGACGCGGTCATCCTGGGCATGTCCAGCCTGGAGCAGCTGGAGCAGAACTT GGCAGCAACAGAGGAAGGGCCCCTGGAGCCGGCTGTCGTGGATGCCTTTAATCAAGCCTGGCATTTGGTT GCTCACGAATGTCCCAACTACTTCCGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC214673 representing NM_003689
Red=Cloning site Green=Tags(s) MLSAASRVVSRAAVHCALRSPPPEARALAMSRPPPPRVASVLGTMEMGRRMDAPASAAAVRAFLERGHTE LDTAFMYSDGQSETILGGLGLGLGGGDCRVKIATKANPWDGKSLKPDSVRSQLETSLKRLQCPQVDLFYL HAPDHGTPVEETLHACQRLHQEGKFVELGLSNYASWEVAEICTLCKSNGWILPTVYQGMYNATTRQVETE LFPCLRHFGLRFYAYNPLAGGLLTGKYKYEDKDGKQPVGRFFGNSWAETYRNRFWKEHHFEAIALVEKAL QAAYGASAPSVTSAALRWMYHHSQLQGAHGDAVILGMSSLEQLEQNLAATEEGPLEPAVVDAFNQAWHLV AHECPNYFR TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_003689 |
| ORF Size | 1077 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_003689.3 |
| RefSeq Size | 1377 bp |
| RefSeq ORF | 1080 bp |
| Locus ID | 8574 |
| UniProt ID | O43488 |
| Domains | aldo_ket_red |
| Protein Families | Druggable Genome |
| MW | 39.4 kDa |
| Gene Summary | The protein encoded by this gene belongs to the aldo/keto reductase (AKR) superfamily and AKR7 family, which are involved in the detoxification of aldehydes and ketones. The AKR7 family consists of 3 genes that are present in a cluster on the p arm of chromosome 1. This protein, thought to be localized in the golgi, catalyzes the NADPH-dependent reduction of succinic semialdehyde to the endogenous neuromodulator, gamma-hydroxybutyrate. It may also function as a detoxication enzyme in the reduction of aflatoxin B1 and 2-carboxybenzaldehyde. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2016] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC214673L3 | Lenti ORF clone of Human aldo-keto reductase family 7, member A2 (aflatoxin aldehyde reductase) (AKR7A2), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC214673L4 | Lenti ORF clone of Human aldo-keto reductase family 7, member A2 (aflatoxin aldehyde reductase) (AKR7A2), mGFP tagged |
CNY 5,890.00 |
|
| RG214673 | AKR7A2 (tGFP-tagged) - Human aldo-keto reductase family 7, member A2 (aflatoxin aldehyde reductase) (AKR7A2) |
CNY 4,370.00 |
|
| SC117811 | AKR7A2 (untagged)-Human aldo-keto reductase family 7, member A2 (aflatoxin aldehyde reductase) (AKR7A2) |
CNY 3,656.00 |
