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SPAG16 (NM_001025436) Human Tagged ORF Clone
CAT＃: RC215059
SPAG16 (Myc-DDK-tagged)-Human sperm associated antigen 16 (SPAG16), transcript variant 2
ORF Plasmid: tGFP
"NM_001025436" in other vectors (4)
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CNY 2,400.00
CNY 3,705.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | PF20; WDR29 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC215059 representing NM_001025436
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGCTCAGCGAGGGATGCCCAGCTCCGCCGTGAGGGTCCTGGAAGAGGCGTTGGGCATGGGTTTGA CGGCAGCCGGGGACGCGAGGGACACGGCGGACGCGGTGGCGGCTGAGGGCGCCTACTACCTGGAACAGGT CACCATAACTGAAGCATCTGAAGATGACTATGAATATGAAGAGATACCAGATGACAATTTTAGCATCCCA GAAGGTGAAGAAGATCTGGCAAAAGCAATTCAGATGGCCCAAGAACAGGCTACAGATACTGAAATTTTGG AACGGAAAACAGTTCTTCCTTCAAAGCATGCAGTACCTGAAGTAATAGAAGACTTTCTCTGCAATTTCTT GATCAAAATGGGAATGACCAGAACTCTTGATTGCTTTCAGTCTGAATGGTATGAGTTAATACAGAAAGGA GTGACTGAACTTAGAACTGTTGGGAATGTTCCAGATGTCTACACCCAGATTATGCTTTTGGAAAATGAGA ACAAAAATTTAAAGAAAGATTTGAAGCACTACAAACAAGCAGCTGAGTATGTTATTTTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC215059 representing NM_001025436
Red=Cloning site Green=Tags(s) MAAQRGMPSSAVRVLEEALGMGLTAAGDARDTADAVAAEGAYYLEQVTITEASEDDYEYEEIPDDNFSIP EGEEDLAKAIQMAQEQATDTEILERKTVLPSKHAVPEVIEDFLCNFLIKMGMTRTLDCFQSEWYELIQKG VTELRTVGNVPDVYTQIMLLENENKNLKKDLKHYKQAAEYVIF TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001025436 |
| ORF Size | 549 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001025436.2, NP_001020607.1 |
| RefSeq Size | 1296 bp |
| RefSeq ORF | 552 bp |
| Locus ID | 79582 |
| UniProt ID | Q8N0X2 |
| MW | 20.4 kDa |
| Gene Summary | Cilia and flagella are comprised of a microtubular backbone, the axoneme, which is organized by the basal body and surrounded by plasma membrane. SPAG16 encodes 2 major proteins that associate with the axoneme of sperm tail and the nucleus of postmeiotic germ cells, respectively (Zhang et al., 2007 [PubMed 17699735]).[supplied by OMIM, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC215059L3 | Lenti ORF clone of Human sperm associated antigen 16 (SPAG16), transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC215059L4 | Lenti ORF clone of Human sperm associated antigen 16 (SPAG16), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
| RG215059 | SPAG16 (tGFP-tagged) - Human sperm associated antigen 16 (SPAG16), transcript variant 2 |
CNY 4,370.00 |
|
| SC302404 | SPAG16 (untagged)-Human sperm associated antigen 16 (SPAG16), transcript variant 2 |
CNY 3,990.00 |
