NuMA (NUMA1) (NM_006185) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	NMP-22; NUMA
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC215124 representing NM_006185. Blue=ORF Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGACACTCCACGCCACCCGGGGGGCTGCACTCCTCTCTTGGGTGAACAGTCTACACGTGGCTGACCCT GTGGAGGCTGTGCTGCAGCTCCAGGACTGCAGCATCTTCATCAAGATCATTGACAGAATCCATGGCACT GAAGAGGGACAGCAAATCTTGAAGCAGCCGGTGTCAGAGAGACTGGACTTTGTGTGCAGTTTTCTGCAG AAAAATCGAAAACATCCCTCTTCCCCAGAATGCCTGGTATCTGCACAGAAGGTGCTAGAGGGATCAGAG CTGGAACTGGCGAAGATGACCATGCTGCTCTTATACCACTCTACCATGAGCTCCAAAAGTCCCAGGGAC TGGGAACAGTTTGAATATAAAATTCAGGCTGAGTTGGCTGTCATTCTTAAATTTGTGCTGGACCATGAG GACGGGCTAAACCTTAATGAGGACCTAGAGAACTTCCTACAGAAAGCTCCTGTGCCTTCTACCTGTTCT AGCACATTCCCTGAAGAGCTCTCCCCACCTAGCCACCAGGCCAAGAGGGAGATTCGCTTCCTAGAGCTA CAGAAGGTTGCCTCCTCTTCCAGTGGGAACAACTTTCTCTCAGGTTCTCCAGCTTCTCCCATGGGTGAT ATCCTGCAGACCCCACAGTTCCAGATGAGACGGCTGAAGAAGCAGCTTGCTGATGAGAGAAGTAATAGG GATGAGCTGGAGCTGGAGCTAGCTGAGAACCGCAAGCTCCTCACCGAGAAGGATGCACAGATAGCCATG ATGCAGCAGCGCATTGACCGCCTAGCCCTGCTGAATGAGAAGCAGGCGGCCAGCCCACTGGAGCCCAAG GAGCTTGAGGAGCTGCGTGACAAGAATGAGAGCCTTACCATGCGGCTGCATGAAACCCTGAAGCAGTGC CAGGACCTGAAGACAGAGAAGAGCCAGATGGATCGCAAAATCAACCAGCTTTCGGAGGAGAATGGAGAC CTTTCCTTTAAGCTGCGGGAGTTTGCCAGTCATCTGCAGCAGCTACAGGATGCCCTCAATGAGCTGACG GAGGAGCACAGCAAGGCCACTCAGGAGTGGCTAGAGAAGCAGGCCCAGCTGGAGAAGGAGCTCAGCGCA GCCCTGCAGGACAAGAAATGCCTTGAAGAGAAGAACGAAATCCTTCAGGGAAAACTTTCACAGCTGGAA GAACACTTGTCCCAGCTGCAGGATAACCCACCCCAGGAGAAGGGCGAGGTGCTGGGTGATGTCTTGCAG CTGGAAACCTTGAAGCAAGAGGCAGCCACTCTTGCTGCAAACAACACACAGCTCCAAGCCAGGGTAGAG ATGCTGGAGACTGAGCGAGGCCAGCAGGAAGCCAAGCTGCTTGCTGAGCGGGGCCACTTCGAAGAAGAA AAGCAGCAGCTGTCTAGCCTGATCACTGACCTGCAGAGCTCCATCTCCAACCTCAGCCAGGCCAAGGAA GAGCTGGAGCAGGCCTCCCAGGCTCATGGGGCCCGGTTGACTGCCCAGGTGGCCTCTCTGACCTCTGAG CTCACCACACTCAATGCCACCATCCAGCAACAGGATCAAGAACTGGCTGGCCTGAAGCAGCAGGCCAAA GAGAAGCAGGCCCAGCTAGCACAGACCCTCCAACAGCAAGAACAGGCCTCCCAGGGCCTCCGCCACCAG GTGGAGCAGCTAAGCAGTAGCCTGAAGCAGAAGGAGCAGCAGTTGAAGGAGGTAGCGGAGAAGCAGGAG GCAACTAGGCAGGACCATGCCCAGCAACTGGCCACTGCTGCAGAGGAGCGAGAGGCCTCCTTAAGGGAG CGGGATGCGGCTCTCAAGCAGCTGGAGGCACTGGAGAAGGAGAAGGCTGCCAAGCTGGAGATTCTGCAG CAGCAACTTCAGGTGGCTAATGAAGCCCGGGACAGTGCCCAGACCTCAGTGACACAGGCCCAGCGGGAG AAGGCAGAGCTGAGCCGGAAGGTGGAGGAACTCCAGGCCTGTGTTGAGACAGCCCGCCAGGAACAGCAT GAGGCCCAGGCCCAGGTTGCAGAGCTAGAGTTGCAGCTGCGGTCTGAGCAGCAAAAAGCAACTGAGAAA GAAAGGGTGGCCCAGGAGAAGGACCAGCTCCAGGAGCAGCTCCAGGCCCTCAAAGAGTCCTTGAAGGTC ACCAAGGGCAGCCTTGAAGAGGAGAAGCGCAGGGCTGCAGATGCCCTGGAAGAGCAGCAGCGTTGTATC TCTGAGCTGAAGGCAGAGACCCGAAGCCTGGTGGAGCAGCATAAGCGGGAACGAAAGGAGCTGGAAGAA GAGAGGGCTGGGCGCAAGGGGCTGGAGGCTCGATTACAGCAGCTTGGGGAGGCCCATCAGGCTGAGACT GAAGTCCTGCGGCGGGAGCTGGCAGAGGCCATGGCTGCCCAGCACACAGCTGAGAGTGAGTGTGAGCAG CTCGTCAAAGAAGTAGCTGCCTGGCGTGAGCGGTATGAGGATAGCCAGCAAGAGGAGGCACAGTATGGC GCCATGTTCCAGGAACAGCTGATGACTTTGAAGGAGGAATGTGAGAAGGCCCGCCAGGAGCTGCAGGAG GCAAAGGAGAAGGTGGCAGGCATAGAATCCCACAGCGAGCTCCAGATAAGCCGGCAGCAGAACGAACTA GCTGAGCTCCATGCCAACCTGGCCAGAGCACTCCAGCAGGTCCAAGAGAAGGAAGTCAGGGCCCAGAAG CTTGCAGATGACCTCTCCACTCTGCAGGAAAAGATGGCTGCCACCAGCAAAGAGGTGGCCCGCTTGGAG ACCTTGGTGCGCAAGGCAGGTGAGCAGCAGGAAACAGCCTCCCGGGAGTTAGTCAAGGAGCCTGCGAGG GCAGGAGACAGACAGCCCGAGTGGCTGGAAGAGCAACAGGGACGCCAGTTCTGCAGCACACAGGCAGCG CTGCAGGCTATGGAGCGGGAGGCAGAGCAGATGGGCAATGAGCTGGAACGGCTGCGGGCCGCGCTGATG GAGAGCCAGGGGCAGCAGCAGGAGGAGCGTGGGCAGCAGGAAAGGGAGGTGGCGCGGCTGACCCAGGAG CGGGGCCGTGCCCAGGCTGACCTTGCCCTGGAGAAGGCGGCCAGAGCAGAGCTTGAGATGCGGCTGCAG AACGCCCTCAACGAGCAGCGTGTGGAGTTCGCTACCCTGCAAGAGGCACTGGCTCATGCCCTGACGGAA AAGGAAGGCAAGGACCAGGAGTTGGCCAAGCTTCGTGGTCTGGAGGCAGCCCAGATAAAAGAGCTGGAG GAACTTCGGCAAACCGTGAAGCAACTGAAGGAACAGCTGGCTAAGAAAGAAAAGGAGCACGCATCTGGC TCAGGAGCCCAATCTGAGGCTGCTGGCAGGACAGAGCCAACAGGCCCCAAGCTGGAGGCACTGCGGGCA GAGGTGAGCAAGCTGGAACAGCAATGCCAGAAGCAGCAGGAGCAGGCTGACAGCCTGGAACGCAGCCTC GAGGCTGAGCGGGCCTCCCGGGCTGAGCGGGACAGTGCTCTGGAGACTCTGCAGGGCCAGTTAGAGGAG AAGGCCCAGGAGCTAGGGCACAGTCAGAGTGCCTTAGCCTCGGCCCAACGGGAGTTGGCTGCCTTCCGC ACCAAGGTACAAGACCACAGCAAGGCTGAAGATGAGTGGAAGGCCCAGGTGGCCCGGGGCCGGCAAGAG GCTGAGAGGAAAAATAGCCTCATCAGCAGCTTGGAGGAGGAGGTGTCCATCCTGAATCGCCAGGTCCTG GAGAAGGAGGGGGAGAGCAAGGAGTTGAAGCGGCTGGTGATGGCCGAGTCAGAGAAGAGCCAGAAGCTG GAGGAGAGGCTGCGCCTGCTGCAGGCAGAGACAGCCAGCAACAGTGCCAGAGCTGCAGAACGCAGCTCT GCTCTGCGGGAGGAGGTGCAGAGCCTCCGGGAGGAGGCTGAGAAACAGCGGGTGGCTTCAGAGAACCTG CGGCAGGAGCTGACCTCACAGGCTGAGCGTGCGGAGGAGCTGGGCCAAGAATTGAAGGCGTGGCAGGAG AAGTTCTTCCAGAAAGAGCAGGCCCTCTCCACCCTGCAGCTCGAGCACACCAGCACACAGGCCCTGGTG AGTGAGCTGCTGCCAGCTAAGCACCTCTGCCAGCAGCTGCAGGCCGAGCAGGCCGCTGCCGAGAAACGC CACCGTGAGGAGCTGGAGCAGAGCAAGCAGGCCGCTGGGGGACTGCGGGCAGAGCTGCTGCGGGCCCAG CGGGAGCTTGGGGAGCTGATTCCTCTGCGGCAGAAGGTGGCAGAGCAGGAGCGAACAGCTCAGCAGCTG CGGGCAGAGAAGGCCAGCTATGCAGAGCAGCTGAGCATGCTGAAGAAGGCGCATGGCCTGCTGGCAGAG GAGAACCGGGGGCTGGGTGAGCGGGCCAACCTTGGCCGGCAGTTTCTGGAAGTGGAGTTGGACCAGGCC CGGGAGAAGTATGTCCAAGAGTTGGCAGCCGTACGTGCTGATGCTGAGACCCGTCTGGCTGAGGTGCAG CGAGAAGCACAGAGCACTGCCCGGGAGCTGGAGGTGATGACTGCCAAGTATGAGGGTGCCAAGGTCAAG GTCCTGGAGGAGAGGCAGCGGTTCCAGGAAGAGAGGCAGAAACTCACTGCCCAGGTGGAGCAGCTAGAG GTATTTCAGAGAGAGCAAACTAAGCAGGTGGAAGAACTGAGTAAGAAACTGGCTGACTCTGACCAAGCC AGCAAGGTGCAGCAGCAGAAGCTGAAGGCTGTCCAGGCTCAGGGAGGCGAGAGCCAGCAGGAGGCCCAG CGCCTCCAGGCCCAGCTGAATGAACTGCAAGCCCAGTTGAGCCAGAAGGAGCAGGCAGCTGAGCACTAT AAGCTGCAGATGGAGAAAGCCAAAACACATTATGATGCCAAGAAGCAGCAGAACCAAGAGCTGCAGGAG CAGCTGCGGAGCCTGGAGCAGCTGCAGAAGGAAAACAAAGAGCTGCGAGCTGAAGCTGAACGGCTGGGC CATGAGCTACAGCAGGCTGGGCTGAAGACCAAGGAGGCTGAACAGACCTGCCGCCACCTTACTGCCCAG GTGCGCAGCCTGGAGGCACAGGTTGCCCATGCAGACCAGCAGCTTCGAGACCTGGGCAAATTCCAGGTG GCAACTGATGCTTTAAAGAGCCGTGAGCCCCAGGCTAAGCCCCAGCTGGACTTGAGTATTGACAGCCTG GATCTGAGCTGCGAGGAGGGGACCCCACTCAGTATCACCAGCAAGCTGCCTCGTACCCAGCCAGACGGC ACCAGCGTCCCTGGAGAACCAGCCTCACCTATCTCCCAGCGCCTGCCCCCCAAGGTAGAATCCCTGGAG AGTCTCTACTTCACTCCCATCCCTGCTCGGAGTCAGGCCCCCCTGGAGAGCAGCCTGGACTCCCTGGGA GACGTCTTCCTGGACTCGGGTCGTAAGACCCGCTCCGCTCGTCGGCGCACCACGCAGATCATCAACATC ACCATGACCAAGAAGCTAGATGTGGAAGAGCCAGACAGCGCCAACTCATCGTTCTACAGCACGCGGTCT GCTCCTGCTTCCCAGGCTAGCCTGCGAGCCACCTCCTCTACTCAGTCTCTAGCTCGCCTGGGTTCTCCC GATTATGGCAACTCAGCCCTGCTCAGCTTGCCTGGCTACCGCCCCACCACTCGCAGTTCTGCTCGTCGT TCCCAGGCCGGGGTGTCCAGTGGGGCCCCTCCAGGAAGGAACAGCTTCTACATGGGCACTTGCCAGGAT GAGCCTGAGCAGCTGGATGACTGGAACCGCATTGCAGAGCTGCAGCAGCGCAATCGAGTGTGCCCCCCA CATCTGAAGACCTGCTATCCCCTGGAGTCCAGGCCTTCCCTGAGCCTGGGCACCATCACAGATGAGGAG ATGAAAACTGGAGACCCCCAAGAGACCCTGCGCCGAGCCAGCATGCAGCCAATCCAGATAGCCGAGGGC ACTGGCATCACCACCCGGCAGCAGCGCAAACGGGTCTCCCTAGAGCCCCACCAGGGCCCTGGAACTCCT GAGTCTAAGAAGGCCACCAGCTGTTTCCCACGCCCCATGACTCCCCGAGACCGACATGAAGGGCGCAAA CAGAGCACTACTGAGGCCCAGAAGAAAGCAGCTCCAGCTTCTACTAAACAGGCTGACCGGCGCCAGTCG ATGGCCTTCAGCATCCTCAACACACCCAAGAAGCTAGGGAACAGCCTTCTGCGGCGGGGAGCCTCAAAG AAGGCCCTGTCCAAGGCTTCCCCCAACACTCGCAGTGGAACCCGCCGTTCTCCGCGCATTGCCACCACC ACAGCCAGCGCCGCCACTGCTGCCGCCATTGGTGCCACCCCTCGAGCCAAGGGCAAGGCAAAGCAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC Protein Sequence (showhide) >Peptide sequence encoded by RC215124 Blue=ORF Red=Cloning site Green=Tag(s) MTLHATRGAALLSWVNSLHVADPVEAVLQLQDCSIFIKIIDRIHGTEEGQQILKQPVSERLDFVCSFLQ KNRKHPSSPECLVSAQKVLEGSELELAKMTMLLLYHSTMSSKSPRDWEQFEYKIQAELAVILKFVLDHE DGLNLNEDLENFLQKAPVPSTCSSTFPEELSPPSHQAKREIRFLELQKVASSSSGNNFLSGSPASPMGD ILQTPQFQMRRLKKQLADERSNRDELELELAENRKLLTEKDAQIAMMQQRIDRLALLNEKQAASPLEPK ELEELRDKNESLTMRLHETLKQCQDLKTEKSQMDRKINQLSEENGDLSFKLREFASHLQQLQDALNELT EEHSKATQEWLEKQAQLEKELSAALQDKKCLEEKNEILQGKLSQLEEHLSQLQDNPPQEKGEVLGDVLQ LETLKQEAATLAANNTQLQARVEMLETERGQQEAKLLAERGHFEEEKQQLSSLITDLQSSISNLSQAKE ELEQASQAHGARLTAQVASLTSELTTLNATIQQQDQELAGLKQQAKEKQAQLAQTLQQQEQASQGLRHQ VEQLSSSLKQKEQQLKEVAEKQEATRQDHAQQLATAAEEREASLRERDAALKQLEALEKEKAAKLEILQ QQLQVANEARDSAQTSVTQAQREKAELSRKVEELQACVETARQEQHEAQAQVAELELQLRSEQQKATEK ERVAQEKDQLQEQLQALKESLKVTKGSLEEEKRRAADALEEQQRCISELKAETRSLVEQHKRERKELEE ERAGRKGLEARLQQLGEAHQAETEVLRRELAEAMAAQHTAESECEQLVKEVAAWRERYEDSQQEEAQYG AMFQEQLMTLKEECEKARQELQEAKEKVAGIESHSELQISRQQNELAELHANLARALQQVQEKEVRAQK LADDLSTLQEKMAATSKEVARLETLVRKAGEQQETASRELVKEPARAGDRQPEWLEEQQGRQFCSTQAA LQAMEREAEQMGNELERLRAALMESQGQQQEERGQQEREVARLTQERGRAQADLALEKAARAELEMRLQ NALNEQRVEFATLQEALAHALTEKEGKDQELAKLRGLEAAQIKELEELRQTVKQLKEQLAKKEKEHASG SGAQSEAAGRTEPTGPKLEALRAEVSKLEQQCQKQQEQADSLERSLEAERASRAERDSALETLQGQLEE KAQELGHSQSALASAQRELAAFRTKVQDHSKAEDEWKAQVARGRQEAERKNSLISSLEEEVSILNRQVL EKEGESKELKRLVMAESEKSQKLEERLRLLQAETASNSARAAERSSALREEVQSLREEAEKQRVASENL RQELTSQAERAEELGQELKAWQEKFFQKEQALSTLQLEHTSTQALVSELLPAKHLCQQLQAEQAAAEKR HREELEQSKQAAGGLRAELLRAQRELGELIPLRQKVAEQERTAQQLRAEKASYAEQLSMLKKAHGLLAE ENRGLGERANLGRQFLEVELDQAREKYVQELAAVRADAETRLAEVQREAQSTARELEVMTAKYEGAKVK VLEERQRFQEERQKLTAQVEQLEVFQREQTKQVEELSKKLADSDQASKVQQQKLKAVQAQGGESQQEAQ RLQAQLNELQAQLSQKEQAAEHYKLQMEKAKTHYDAKKQQNQELQEQLRSLEQLQKENKELRAEAERLG HELQQAGLKTKEAEQTCRHLTAQVRSLEAQVAHADQQLRDLGKFQVATDALKSREPQAKPQLDLSIDSL DLSCEEGTPLSITSKLPRTQPDGTSVPGEPASPISQRLPPKVESLESLYFTPIPARSQAPLESSLDSLG DVFLDSGRKTRSARRRTTQIINITMTKKLDVEEPDSANSSFYSTRSAPASQASLRATSSTQSLARLGSP DYGNSALLSLPGYRPTTRSSARRSQAGVSSGAPPGRNSFYMGTCQDEPEQLDDWNRIAELQQRNRVCPP HLKTCYPLESRPSLSLGTITDEEMKTGDPQETLRRASMQPIQIAEGTGITTRQQRKRVSLEPHQGPGTP ESKKATSCFPRPMTPRDRHEGRKQSTTEAQKKAAPASTKQADRRQSMAFSILNTPKKLGNSLLRRGASK KALSKASPNTRSGTRRSPRIATTTASAATAAAIGATPRAKGKAKH TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms	CHROMATOGRAMS Sequencher program is needed, download here.
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_006185
ORF Size	6345 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_006185.4
RefSeq Size	7182 bp
RefSeq ORF	6348 bp
Locus ID	4926
UniProt ID	Q14980
Domains	M
MW	238.3 kDa
Gene Summary	This gene encodes a large protein that forms a structural component of the nuclear matrix. The encoded protein interacts with microtubules and plays a role in the formation and organization of the mitotic spindle during cell division. Chromosomal translocation of this gene with the RARA (retinoic acid receptor, alpha) gene on chromosome 17 have been detected in patients with acute promyelocytic leukemia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2013]

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