United States
Germany
Japan
United Kingdom
China
ARHGEF5 (NM_005435) Human Tagged ORF Clone
CAT＃: RC215166
ARHGEF5 (Myc-DDK-tagged)-Human Rho guanine nucleotide exchange factor (GEF) 5 (ARHGEF5)
ORF Plasmid: tGFP
"NM_005435" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 10,928.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | GEF5; P60; TIM; TIM1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC215166 representing NM_005435
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGCTGAGGAGGCCCAGCGTGGAGCCTCTCCTCCCATCTCTGCCATAGAGGAATTCAGCATTATCC CTGAGGCTCCCATGAGGAGCAGCCAGGTCTCTGCCTTGGGGCTTGAAGCTCAAGAAGATGAGGACCCATC CTATAAGTGGAGAGAGGAACACAGACTCTCAGCAACTCAGCAGAGTGAGTTAAGGGATGTGTGTGACTAT GCGATTGAGACGATGCCCTCTTTTCCCAAGGAAGGTTCTGCAGATGTGGAGCCCAATCAGGAAAGCCTTG TGGCTGAGGCCTGTGACACTCCGGAACACTGGGAGGCAGTACCCCAGAGCCTAGCAGGCCGACAAGCAAG GACTCTAGCTCCCCCAGAGCTCTGGGCCTGCCCCATTCAGAGTGAGCATCTAGACATGGCCCCATTTTCC AGTGACCTGGGAAGCGAAGAAGAGGAGGTGGAATTTTGGCCAGGACTTACTTCTTTGACATTGGGATCTG GACAGGCAGAAGAAGAAGAGGAAACCTCTTCAGATAACTCTGGTCAGACCAGATATTATTCTCCCTGCGA AGAGCATCCTGCAGAGACCAACCAGAATGAAGGCTCTGAAAGTGGGACTATCAGGCAGGGGGAAGAGCTG CCACCTGAGGAGCTGCAGGAAAGTCAAGGGCTCTTGCATCCCCAGGAGGTCCAAGTTCTGGAGGAGCAGG GACAGCAGGAAGCAGGATTTCGGGGGGAAGGAACTCTGAGGGAGGATGTTTGTGCCGATGGGCTATTAGG GGAGGAACAGATGATAGAGCAGGTTAATGATGAAAAGGGAGAACAGAAGCAAAAACAGGAACAGGTACAA GATGTGATGCTTGGGAGACAAGGAGAAAGAATGGGGCTCACTGGGGAGCCAGAGGGTCTGAATGACGGTG AGTGGGAGCAGGAGGATATGGAGAGGAAGGCTCAGGGTCAGGGAGGTCCAGAACAGGGAGAAGAGAGGAA GAGGGAGCTGCAGGTGCCAGAAGAGAATAGGGCGGACTCTCAGGACGAAAAGAGTCAAACCTTTTTGGGA AAATCAGAGGAAGTAACTGGAAAGCAAGAAGATCATGGTATAAAGGAGAAAGGGGTGCCAGTCAGCGGGC AGGAGGCGAAAGAGCCAGAGAGTTGGGATGGGGGCAGGCTGGGGGCAGTGGGAAGAGCGAGGAGCAGGGA AGAGGAGAATGAGCATCATGGGCCTTCAATGCCCGCTCTGATAGCCCCTGAGGACTCTCCTCACTGTGAC CTGTTTCCAGGTGCCTCATATCTCATGACTCAGATTCCCGGGACTCAGACAGAGTCCAGGGCTGAGGAAC TGTCCCCCGCAGCTCTGTCTCCCTCGCTAGAGCCCATCAGGTGCTCTCACCAGCCCATTTCTCTACTGGG CTCCTTTTTGACTGAGGAGTCACCTGACAAGGAAATAGATCAAAACAGCCAGCAAGAGGAATCCAGGCTG AGGAAGGGAACAGTGTCCAGCCAAGGGACTGAGGTGGTCTTTGCCAGTGCATCTGTGACTCCTCCAAGGA CACCAGATTCAGCTCCTCCCAGTCCTGCTGAAGCCTACCCCATCACACCTGCCTCGGTATCTGCCAGGCC CCCAGTTGCCTTTCCCAGGAGGGAAACCTCTTGTGCTGCACGTGCTCCAGAAACTGCCAGTGCCCCTCTC TCAATGGATGACCCATCTCCCTGTGGGACTTCTGAGATGTGCCCGGCTGCCCTCTATGGCTTCCCCTCCA CCGGGACCAGCCCTCCGAGGCCCCCAGCCAACTCCACAGGCACCGTCCAGCACTTACGGAGTGACTCCTT CCCTGGTTCTCACAGGACAGAGCAGACTCCAGACCTGGTGGGAATGTTGCTTTCCTACTCCCACTCAGAG CTGCCCCAGAGGCCCCCCAAACCTGCCATCTACAGCTCTGTGACCCCAAGAAGGGACAGAAGGAGTGGTA GGGACTACAGCACCGTTTCAGCATCCCCTACTGCCTTATCCACGCTGAAGCAGGACTCTCAAGAATCCAT CTCAAATCTAGAGAGACCCAGCAGTCCTCCCAGCATCCAGCCCTGGGTCTCCCCACATAATCCAGCCTTT GCCACAGAGTCTCCCGCCTACGGTTCTTCCCCATCCTTTGTCTCCATGGAGGATGTGAGGATCCACGAAC CTCTGCCCCCTCCTCCCCCACAGAGGAGGGACACCCATCCCTCCGTGGTGGAGACAGATGGCCATGCTCG TGTAGTGGTTCCCACGCTGAAGCAGCATAGCCACCCTCCTCCATTGGCCCTAGGTTCAGGGCTGCATGCC CCCCATAAAGGCCCACTTCCCCAAGCCTCTGACCCCGCTGTGGCCAGGCAGCACCGACCTCTGCCATCTA CCCCAGACAGCTCCCACCATGCTCAGGCCACCCCCAGGTGGAGATACAACAAGCCGCTACCCCCTACCCC TGATTTGCCGCAGCCCCACCTTCCTCCCATTTCTGCTCCTGGTAGCTCAAGGATCTACAGGCCTCTACCC CCACTACCCATCATAGACCCTCCCACCGAACCACCCCCATTGCCCCCAAAGTCCAGGGGGAGGAGCAGGA GCACTCGGGGAGGACATATGAACTCAGGGGGTCATGCCAAAACAAGACCTGCTTGTCAAGACTGGACAGT CCCCCTCCCTGCCTCTGCTGGACGCACCTCCTGGCCCCCGGCCACAGCTAGATCAACAGAGTCTTTCACT TCCACCAGCAGGAGTAAGAGCGAAGTGTCCCCTGGCATGGCTTTCAGCAACATGACAAACTTCCTATGCC CCTCTTCCCCTACCACTCCCTGGACTCCGGAGCTCCAGGGACCCACCTCTAAGGATGAAGCAGGGGTCTC AGAACACCCTGAGGCCCCTGCGAGAGAACCTTTGAGAAGGACAACCCCTCAGCAAGGAGCCAGTGGCCCA GGGAGGTCACCTGTGGGCCAAGCAAGGCAGCCAGAAAAACCCAGCCATCTGCACCTGGAGAAGGCGTCCA GCTGGCCCCACAGGCGGGACTCAGGGAGGCCACCAGGGGACAGCAGTGGACAGGCTGTGGCTCCTAGTGA GGGGGCCAACAAGCACAAGGGCTGGAGCCGGCAGGGCCTGCGCAGACCTTCCATCTTGCCTGAGGGCTCT TCAGATTCAAGAGGTCCAGCCGTGGAGAAACATCCGGGACCCTCAGACACTGTTGTTTTTCGGGAGAAAA AACCAAAGGAGGTGATGGGAGGCTTTTCAAGACGCTGCTCCAAACTCATCAACTCCTCCCAGCTGCTTTA CCAGGAGTATAGTGATGTTGTCCTGAATAAGGAGATCCAGAGCCAGCAGCGGCTGGAGAGCCTGTCCGAG ACACCCGGGCCTAGCTCTCCGCGGCAGCCTCGGAAGGCCCTGGTCTCCTCCGAGTCGTACCTGCAGCGGC TCTCCATGGCCTCCAGCGGCTCCCTCTGGCAGGAAATCCCCGTGGTGCGCAACAGCACCGTGCTGCTCTC CATGACCCATGAAGACCAAAAGCTGCAAGAGGTCAAATTTGAGCTGATTGTGTCAGAGGCCTCCTACCTG CGCAGTCTAAACATAGCTGTGGATCATTTCCAACTTTCAACTTCACTCCGGGCCACACTTTCCAACCAGG AGCACCAATGGCTCTTCTCTCGTTTACAGGATGTGCGAGACGTCAGCGCCACGTTCCTTTCAGACCTGGA AGAGAACTTTGAGAACAATATCTTCTCCTTCCAAGTATGTGACGTAGTCCTGAACCACGCCCCAGACTTC CGCCGGGTCTACCTGCCTTATGTCACCAACCAGACCTATCAGGAACGCACCTTCCAGAGCCTGATGAATA GCAACAGCAATTTCCGGGAGGTCTTGGAGAAGCTGGAGAGCGACCCCGTCTGCCAGCGCCTTTCCCTCAA GTCCTTTCTGATTCTGCCCTTCCAACGCATCACCCGCCTCAAACTGCTGCTCCAGAACATTCTGAAGAGA ACACAGCCTGGCTCCTCGGAGGAGGCAGAGGCCACGAAGGCACACCACGCCCTGGAGCAGCTGATCCGGG ACTGCAATAACAATGTCCAGAGTATGCGACGGACAGAGGAACTAATCTACCTGAGCCAGAAGATTGAGTT TGAGTGCAAAATATTCCCGCTCATTTCTCAGTCACGCTGGCTGGTGAAAAGTGGGGAGCTGACAGCCTTG GAGTTCAGTGCTTCCCCAGGGCTACGAAGGAAGCTGAACACGCGTCCAGTCCACCTGCACCTCTTCAATG ACTGTCTGCTGCTGTCTCGGCCCCGAGAGGGTAGCCGATTCCTGGTATTTGACCATGCTCCCTTCTCCTC CATTCGGGGGGAAAAGTGTGAAATGAAGCTACATGGACCTCACAAAAACCTGTTCCGACTCTTTCTGCGG CAGAACACTCAGGGCGCCCAGGCCGAGTTCCTCTTCCGCACGGAGACTCAAAGTGAAAAGCTTCGGTGGA TCTCAGCCTTGGCCATGCCAAGAGAGGAGTTGGACCTTCTGGAGTGTTACAACTCCCCCCAGGTACAGTG CCTTCGAGCCTACAAGCCCCGAGAGAATGATGAATTGGCACTGGAGAAAGCCGACGTGGTGATGGTGACT CAGCAGAGCAGTGACGGCTGGCTGGAGGGCGTGAGGCTCTCAGACGGGGAGCGAGGCTGGTTTCCTGTGC AGCAGGTGGAGTTCATTTCCAACCCAGAGGTCCGTGCACAGAACCTGAAGGAAGCTCATCGAGTCAAGAC TGCCAAACTACAGCTGGTGGAACAGCAAGCC AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA >RC215166 representing NM_005435
Red=Cloning site Green=Tags(s) MEAEEAQRGASPPISAIEEFSIIPEAPMRSSQVSALGLEAQEDEDPSYKWREEHRLSATQQSELRDVCDY AIETMPSFPKEGSADVEPNQESLVAEACDTPEHWEAVPQSLAGRQARTLAPPELWACPIQSEHLDMAPFS SDLGSEEEEVEFWPGLTSLTLGSGQAEEEEETSSDNSGQTRYYSPCEEHPAETNQNEGSESGTIRQGEEL PPEELQESQGLLHPQEVQVLEEQGQQEAGFRGEGTLREDVCADGLLGEEQMIEQVNDEKGEQKQKQEQVQ DVMLGRQGERMGLTGEPEGLNDGEWEQEDMERKAQGQGGPEQGEERKRELQVPEENRADSQDEKSQTFLG KSEEVTGKQEDHGIKEKGVPVSGQEAKEPESWDGGRLGAVGRARSREEENEHHGPSMPALIAPEDSPHCD LFPGASYLMTQIPGTQTESRAEELSPAALSPSLEPIRCSHQPISLLGSFLTEESPDKEIDQNSQQEESRL RKGTVSSQGTEVVFASASVTPPRTPDSAPPSPAEAYPITPASVSARPPVAFPRRETSCAARAPETASAPL SMDDPSPCGTSEMCPAALYGFPSTGTSPPRPPANSTGTVQHLRSDSFPGSHRTEQTPDLVGMLLSYSHSE LPQRPPKPAIYSSVTPRRDRRSGRDYSTVSASPTALSTLKQDSQESISNLERPSSPPSIQPWVSPHNPAF ATESPAYGSSPSFVSMEDVRIHEPLPPPPPQRRDTHPSVVETDGHARVVVPTLKQHSHPPPLALGSGLHA PHKGPLPQASDPAVARQHRPLPSTPDSSHHAQATPRWRYNKPLPPTPDLPQPHLPPISAPGSSRIYRPLP PLPIIDPPTEPPPLPPKSRGRSRSTRGGHMNSGGHAKTRPACQDWTVPLPASAGRTSWPPATARSTESFT STSRSKSEVSPGMAFSNMTNFLCPSSPTTPWTPELQGPTSKDEAGVSEHPEAPAREPLRRTTPQQGASGP GRSPVGQARQPEKPSHLHLEKASSWPHRRDSGRPPGDSSGQAVAPSEGANKHKGWSRQGLRRPSILPEGS SDSRGPAVEKHPGPSDTVVFREKKPKEVMGGFSRRCSKLINSSQLLYQEYSDVVLNKEIQSQQRLESLSE TPGPSSPRQPRKALVSSESYLQRLSMASSGSLWQEIPVVRNSTVLLSMTHEDQKLQEVKFELIVSEASYL RSLNIAVDHFQLSTSLRATLSNQEHQWLFSRLQDVRDVSATFLSDLEENFENNIFSFQVCDVVLNHAPDF RRVYLPYVTNQTYQERTFQSLMNSNSNFREVLEKLESDPVCQRLSLKSFLILPFQRITRLKLLLQNILKR TQPGSSEEAEATKAHHALEQLIRDCNNNVQSMRRTEELIYLSQKIEFECKIFPLISQSRWLVKSGELTAL EFSASPGLRRKLNTRPVHLHLFNDCLLLSRPREGSRFLVFDHAPFSSIRGEKCEMKLHGPHKNLFRLFLR QNTQGAQAEFLFRTETQSEKLRWISALAMPREELDLLECYNSPQVQCLRAYKPRENDELALEKADVVMVT QQSSDGWLEGVRLSDGERGWFPVQQVEFISNPEVRAQNLKEAHRVKTAKLQLVEQQA SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_005435 |
| ORF Size | 4791 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_005435.4 |
| RefSeq Size | 5505 bp |
| RefSeq ORF | 4794 bp |
| Locus ID | 7984 |
| UniProt ID | Q12774 |
| Domains | RhoGEF, SH3, PH |
| Protein Families | Druggable Genome |
| MW | 176.8 kDa |
| Gene Summary | Rho GTPases play a fundamental role in numerous cellular processes initiated by extracellular stimuli that work through G protein coupled receptors. The encoded protein may form a complex with G proteins and stimulate Rho-dependent signals. This protein may be involved in the control of cytoskeletal organization. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC215166L3 | Lenti-ORF clone of ARHGEF5 (Myc-DDK-tagged)-Human Rho guanine nucleotide exchange factor (GEF) 5 (ARHGEF5) |
CNY 13,970.00 |
|
| RC215166L4 | Lenti-ORF clone of ARHGEF5 (mGFP-tagged)-Human Rho guanine nucleotide exchange factor (GEF) 5 (ARHGEF5) |
CNY 13,970.00 |
|
| RG215166 | ARHGEF5 (tGFP-tagged) - Human Rho guanine nucleotide exchange factor (GEF) 5 (ARHGEF5) |
CNY 13,300.00 |
|
| SC308978 | ARHGEF5 (untagged)-Human Rho guanine nucleotide exchange factor (GEF) 5 (ARHGEF5) |
CNY 10,450.00 |
其它ARHGEF5产品
