United States
Germany
Japan
United Kingdom
China
TLR5 (NM_003268) Human Tagged ORF Clone
CAT＃: RC215293
TLR5 (Myc-DDK-tagged)-Human toll-like receptor 5 (TLR5)
ORF Plasmid: tGFP
"NM_003268" in other vectors (6)
Need custom modification / cloning service?
Get a free quote
CNY 6,288.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | MELIOS; SLE1; SLEB1; TIL3 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC215293 representing NM_003268
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGAGACCACCTGGACCTTCTCCTAGGAGTGGTGCTCATGGCCGGTCCTGTGTTTGGAATTCCTTCCT GCTCCTTTGATGGCCGAATAGCCTTTTATCGTTTCTGCAACCTCACCCAGGTCCCCCAGGTCCTCAACAC CACTGAGAGGCTCCTGCTGAGCTTCAACTATATCAGGACAGTCACTGCTTCATCCTTCCCCTTTCTGGAA CAGCTGCAGCTGCTGGAGCTCGGGAGCCAGTATACCCCCTTGACTATTGACAAGGAGGCCTTCAGAAACC TGCCCAACCTTAGAATCTTGGACCTGGGAAGTAGTAAGATATACTTCTTGCATCCAGATGCTTTTCAGGG ACTGTTCCATCTGTTTGAACTTAGACTGTATTTCTGTGGTCTCTCTGATGCTGTATTGAAAGATGGTTAT TTCAGAAATTTAAAGGCTTTAACTCGCTTGGATCTATCCAAAAATCAGATTCGTAGCCTTTACCTTCATC CTTCATTTGGGAAGTTGAATTCCTTAAAGTCCATAGATTTTTCCTCCAACCAAATATTCCTTGTATGTGA ACATGAGCTCGAGCCCCTACAAGGGAAAACGCTCTCCTTTTTTAGCCTCGCAGCTAATAGCTTGTATAGC AGAGTCTCAGTGGACTGGGGAAAATGTATGAACCCATTCAGAAACATGGTGCTGGAGATACTAGATGTTT CTGGAAATGGCTGGACAGTGGACATCACAGGAAACTTTAGCAATGCCATCAGCAAAAGCCAGGCCTTCTC TTTGATTCTTGCCCACCACATCATGGGTGCCGGGTTTGGCTTCCATAACATCAAAGATCCTGACCAGAAC ACATTTGCTGGCCTGGCCAGAAGTTCAGTGAGACACCTGGATCTTTCACATGGGTTTGTCTTCTCCCTGA ACTCACGAGTCTTTGAGACACTCAAGGATTTGAAGGTTCTGAACCTTGCCTACAACAAGATAAATAAGAT TGCAGATGAAGCATTTTACGGACTTGACAACCTCCAAGTTCTCAATTTGTCATATAACCTTCTGGGGGAA CTTTACAGTTCGAATTTCTATGGACTACCTAAGGTAGCCTACATTGATTTGCAAAAGAATCACATTGCAA TAATTCAAGACCAAACATTCAAATTCCTGGAAAAATTACAGACCTTGGATCTCCGAGACAATGCTCTTAC AACCATTCATTTTATTCCAAGCATACCCGATATCTTCTTGAGTGGCAATAAACTAGTGACTTTGCCAAAG ATCAACCTTACAGCGAACCTCATCCACTTATCAGAAAACAGGCTAGAAAATCTAGATATTCTCTACTTTC TCCTACGGGTACCTCATCTCCAGATTCTCATTTTAAATCAAAATCGCTTCTCCTCCTGTAGTGGAGATCA AACCCCTTCAGAGAATCCCAGCTTAGAACAGCTTTTCCTTGGAGAAAATATGTTGCAACTTGCCTGGGAA ACTGAGCTCTGTTGGGATGTTTTTGAGGGACTTTCTCATCTTCAAGTTCTGTATTTGAATCATAACTATC TTAATTCCCTTCCACCAGGAGTATTTAGCCATCTGACTGCATTAAGGGGACTAAGCCTCAACTCCAACAG GCTGACAGTTCTTTCTCACAATGATTTACCTGCTAATTTAGAGATCCTGGACATATCCAGGAACCAGCTC CTAGCTCCTAATCCTGATGTATTTGTATCACTTAGTGTCTTGGATATAACTCATAACAAGTTCATTTGTG AATGTGAACTTAGCACTTTTATCAATTGGCTTAATCACACCAATGTCACTATAGCTGGGCCTCCTGCAGA CATATATTGTGTGTACCCTGACTCGCTCTCTGGGGTTTCCCTCTTCTCTCTTTCCACGGAAGGTTGTGAT GAAGAGGAAGTCTTAAAGTCCCTAAAGTTCTCCCTTTTCATTGTATGCACTGTCACTCTGACTCTGTTCC TCATGACCATCCTCACAGTCACAAAGTTCCGGGGCTTCTGTTTTATCTGTTATAAGACAGCCCAGAGACT GGTGTTCAAGGACCATCCCCAGGGCACAGAACCTGATATGTACAAATATGATGCCTATTTGTGCTTCAGC AGCAAAGACTTCACATGGGTGCAGAATGCTTTGCTCAAACACCTGGACACTCAATACAGTGACCAAAACA GATTCAACCTGTGCTTTGAAGAAAGAGACTTTGTCCCAGGAGAAAACCGCATTGCCAATATCCAGGATGC CATCTGGAACAGTAGAAAGATCGTTTGTCTTGTGAGCAGACACTTCCTTAGAGATGGCTGGTGCCTTGAA GCCTTCAGTTATGCCCAGGGCAGGTGCTTATCTGACCTTAACAGTGCTCTCATCATGGTGGTGGTTGGGT CCTTGTCCCAGTACCAGTTGATGAAACATCAATCCATCAGAGGCTTTGTACAGAAACAGCAGTATTTGAG GTGGCCTGAGGATCTCCAGGATGTTGGCTGGTTTCTTCATAAACTCTCTCAACAGATACTAAAGAAAGAA AAAGAAAAGAAGAAAGACAATAACATTCCGTTGCAAACTGTAGCAACCATCTCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC215293 representing NM_003268
Red=Cloning site Green=Tags(s) MGDHLDLLLGVVLMAGPVFGIPSCSFDGRIAFYRFCNLTQVPQVLNTTERLLLSFNYIRTVTASSFPFLE QLQLLELGSQYTPLTIDKEAFRNLPNLRILDLGSSKIYFLHPDAFQGLFHLFELRLYFCGLSDAVLKDGY FRNLKALTRLDLSKNQIRSLYLHPSFGKLNSLKSIDFSSNQIFLVCEHELEPLQGKTLSFFSLAANSLYS RVSVDWGKCMNPFRNMVLEILDVSGNGWTVDITGNFSNAISKSQAFSLILAHHIMGAGFGFHNIKDPDQN TFAGLARSSVRHLDLSHGFVFSLNSRVFETLKDLKVLNLAYNKINKIADEAFYGLDNLQVLNLSYNLLGE LYSSNFYGLPKVAYIDLQKNHIAIIQDQTFKFLEKLQTLDLRDNALTTIHFIPSIPDIFLSGNKLVTLPK INLTANLIHLSENRLENLDILYFLLRVPHLQILILNQNRFSSCSGDQTPSENPSLEQLFLGENMLQLAWE TELCWDVFEGLSHLQVLYLNHNYLNSLPPGVFSHLTALRGLSLNSNRLTVLSHNDLPANLEILDISRNQL LAPNPDVFVSLSVLDITHNKFICECELSTFINWLNHTNVTIAGPPADIYCVYPDSLSGVSLFSLSTEGCD EEEVLKSLKFSLFIVCTVTLTLFLMTILTVTKFRGFCFICYKTAQRLVFKDHPQGTEPDMYKYDAYLCFS SKDFTWVQNALLKHLDTQYSDQNRFNLCFEERDFVPGENRIANIQDAIWNSRKIVCLVSRHFLRDGWCLE AFSYAQGRCLSDLNSALIMVVVGSLSQYQLMKHQSIRGFVQKQQYLRWPEDLQDVGWFLHKLSQQILKKE KEKKKDNNIPLQTVATIS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_003268 |
| ORF Size | 2574 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_003268.6 |
| RefSeq Size | 3369 bp |
| RefSeq ORF | 2577 bp |
| Locus ID | 7100 |
| UniProt ID | O60602 |
| Domains | TIR, LRRCT, LRR, LRR_TYP, LRR_SD22, LRR_PS |
| Protein Families | Druggable Genome, Transmembrane |
| Protein Pathways | Pathogenic Escherichia coli infection, Toll-like receptor signaling pathway |
| MW | 97.77 kDa |
| Gene Summary | This gene encodes a member of the toll-like receptor (TLR) family, which plays a fundamental role in pathogen recognition and activation of innate immune responses. These receptors recognize distinct pathogen-associated molecular patterns that are expressed on infectious agents. The protein encoded by this gene recognizes bacterial flagellin, the principal component of bacterial flagella and a virulence factor. The activation of this receptor mobilizes the nuclear factor NF-kappaB, which in turn activates a host of inflammatory-related target genes. Mutations in this gene have been associated with both resistance and susceptibility to systemic lupus erythematosus, and susceptibility to Legionnaire disease.[provided by RefSeq, Dec 2009] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC215293L1 | Lenti ORF clone of Human toll-like receptor 5 (TLR5), Myc-DDK-tagged |
CNY 8,688.00 |
|
| RC215293L2 | Lenti ORF clone of Human toll-like receptor 5 (TLR5), mGFP tagged |
CNY 5,890.00 |
|
| RC215293L3 | Lenti ORF clone of Human toll-like receptor 5 (TLR5), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC215293L4 | Lenti ORF clone of Human toll-like receptor 5 (TLR5), mGFP tagged |
CNY 8,688.00 |
|
| RG215293 | TLR5 (tGFP-tagged) - Human toll-like receptor 5 (TLR5) |
CNY 7,888.00 |
|
| SC112789 | TLR5 (untagged)-Human toll-like receptor 5 (TLR5) |
CNY 6,288.00 |
