RYK (NM_002958) Human Tagged Lenti ORF Clone

CAT#: RC215509L1

  • LentiORF®

Lenti ORF clone of Human RYK receptor-like tyrosine kinase (RYK), transcript variant 2, Myc-DDK-tagged



  "NM_002958" in other vectors (7)

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CNY 6,920.00

CNY 7,220.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
Lenti-vpak packaging kit - packaging plasmids and transfection reagent
    • 10 reactions

CNY 5,420.00


Forward primer to sequence targets cloned in expression vectors.
    • 1 nmol

CNY 570.00


Reverse primer to sequence targets cloned in expression vectors.
    • 1 nmol

CNY 570.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 100 ul

CNY 600.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Synonyms D3S3195; JTK5; JTK5A; RYK1
Vector pLenti-C-Myc-DDK
E. coli Selection Chloramphenicol (34 ug/mL)
Mammalian Cell Selection None
Sequence Data
The ORF insert of this clone is exactly the same as(RC215509).
Restriction Sites SgfI-RsrII      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_002958
ORF Size 1821 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_002958.2
RefSeq Size 2951 bp
RefSeq ORF 1824 bp
Locus ID 6259
UniProt ID P34925
Domains pkinase, TyrKc, S_TKc, WIF
Protein Families Druggable Genome, Protein Kinase, Transmembrane
MW 67.6 kDa
Gene Summary The protein encoded by this gene is an atypical member of the family of growth factor receptor protein tyrosine kinases, differing from other members at a number of conserved residues in the activation and nucleotide binding domains. This gene product belongs to a subfamily whose members do not appear to be regulated by phosphorylation in the activation segment. It has been suggested that mediation of biological activity by recruitment of a signaling-competent auxiliary protein may occur through an as yet uncharacterized mechanism. The encoded protein has a leucine-rich extracellular domain with a WIF-type Wnt binding region, a single transmembrane domain, and an intracellular tyrosine kinase domain. This protein is involved in stimulating Wnt signaling pathways such as the regulation of axon pathfinding. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Feb 2012]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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