SPHKAP (NM_030623) Human Tagged ORF Clone
CAT#: RC215958
SPHKAP (Myc-DDK-tagged)-Human SPHK1 interactor, AKAP domain containing (SPHKAP), transcript variant 2
ORF Plasmid: tGFP
"NM_030623" in other vectors (2)
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CNY 11,232.00
Cited in 1 publication. |
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | SKIP |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC215958 representing NM_030623
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATGGCAACTCCCTGCTCTCGGTACCAAGCAACTTGGAGTCATCACGGATGTATGACGTTTTGGAAC CGCAGCAGGGCAGAGGCTGTGGCAGCTCAGGAAGCGGCCCGGGGAACTCCATCACAGCCTGTAAGAAGGT TCTTCGCAGCAATAGCCTGCTGGAGTCAACAGACTACTGGTTGCAGAATCAGAGGATGCCCTGCCAAATT GGTTTTGTAGAAGACAAGTCTGAAAACTGTGCTTCTGTCTGCTTTGTGAATCTTGGTGTGAACAAGGATG AATGCAGCACAGAGCACCTGCAACAGAAACTGGTCAACGTTTCACCAGATCTTCCAAAACTTATCAGTTC CATGAATGTCCAACAACCAAAAGAAAATGAAATTGTTGTCCTAAGTGGGTTAGCCTCTGGAAATCTCCAG GCAGATTTTGAAGTTTCACAGTGCCCTTGGCTGCCAGATATCTGCTTGGTCCAATGTGCAAGAGGGAACA GACCAAACAGTACCAACTGCATCATCTTTGAAATCAACAAATTTCTGATTGGTCTGGAACTGGTGCAGGA GCGACAGCTCCACCTGGAAACAAACATCTTGAAACTGGAGGATGACACGAACTGTTCCTTATCTTCAATC GAGGAAGACTTTCTCACCGCTTCTGAGCACTTGGAGGAGGAAAGCGAGGTGGATGAATCTAGGAACGATT ATGAAAATATAAATGTCTCAGCCAATGTTTTGGAAAGTAAACAGCTAAAGGGAGCCACCCAGGTGGAATG GAATTGCAACAAGGAAAAGTGGCTTTATGCTTTGGAAGACAAATACATCAACAAATATCCCACACCATTG ATTAAAACAGAACGATCTCCAGAAAACCTAACAAAGAACACAGCCTTGCAGAGTCTAGATCCCTCAGCCA AGCCATCACAGTGGAAAAGAGAAGCTGTGGGGAATGGGAGACAAGCCACACATTATTATCATTCAGAAGC TTTTAAAGGTCAAATGGAAAAATCACAGGCACTGTATATTCCAAAAGATGCTTATTTCTCCATGATGGAT AAAGATGTACCTTCTGCATGTGCTGTGGCAGAGCAGAGAAGCAACCTAAACCCAGGAGACCATGAAGACA CAAGAAACGCTCTCCCTCCTAGACAAGATGGAGAAGTCACCACTGGCAAGTATGCTACAAATTTAGCAGA ATCCGTGCTGCAGGATGCATTTATTAGATTATCTCAATCTCAGTCCACATTACCCCAGGAATCTGCAGTC AGTGTTTCTGTAGGAAGTTCTCTGCTTCCCAGTTGCTATTCCACAAAAGATACAGTGGTTTCTCGGTCAT GGAATGAGCTCCCCAAAATCGTCGTTGTTCAGAGTCCAGATGGCAGTGATGCTGCCCCACAGCCAGGCAT CTCCTCCTGGCCTGAGATGGAAGTCTCTGTTGAAACCTCAAGCATCCCCTCTGGAGAGAACTCCAGCAGA CAACCCCAGAGTGCTCTAGAAGTGGCGTTAGCTTGTGCAGCCACTGTGATTGGAACTATTTCCAGTCCAC AGGCCACAGAAAGACTCAAAATGGAGCAAGTGGTCTCGAACTTTCCCCCAGGGAGCAGTGGTGCACTGCA AACTCAAGCACCCCAAGGACTCAAGGAACCTTCCATCAATGAGTACTCCTTTCCATCTGCTTTGTGTGGC ATGACTCAGGTGGCCAGTGCCGTGGCTGTCTGTGGTCTGGGTGAAAGAGAAGAGGTGACATGCTCAGTGG CTCCAAGTGGTAGCCTCCCGCCTGCAGCTGAGGCTTCTGAAGCCATGCCCCCACTTTGTGGTTTAGCAAG CATGGAGCTTGGCAAGGAAGCCATTGCCAAGGGATTGCTCAAGGAGGCTGCTCTGGTTTTAACAAGGCCT AATACCTACAGCAGCATTGGAGACTTTCTGGACTCCATGAACAGGAGAATCATGGAAACTGCTTCAAAGT CTCAGACCCTGTGCTCAGAAAATGTCGTCAGGAATGAACTGGCACATACCCTGTCCAATGTTATCCTGAG GCATTCCATTGATGAAGTTCACCACAAAAATATGATAATCGACCCCAATGACAACAGGCATTCATCTGAA ATTCTGGACACCTTAATGGAAAGTACAAATCAACTGCTTTTAGATGTGATATGCTTCACGTTCAAGAAGA TGAGTCATATTGTACGGCTTGGTGAATGTCCTGCTGTCCTTTCTAAGGAGACCATCAGAAGGAGGGAGAC AGAACCAAGCTGCCAGCCATCTGATCCGGGTGCTAGTCAAGCTTGGACAAAAGCCACTGAATCCTCCAGC AGCTCTCCACTTAGCAATTCACACAACACGAGTCTTGTCATCAACAATCTTGTGGATGGCATGTATTCAA AACAAGACAAGGGTGGAGTGAGGCCAGGCCTCTTCAAGAACCCCACGCTGCAGTCACAATTATCACGTAG TCACAGAGTGCCCGATTCTTCAACTGCTACAACATCCTCCAAGGAAATATATCTGAAAGGAATAGCAGGA GAGGATACAAAAAGCCCTCATCACAGTGAGAATGAATGCAGAGCCTCTTCCGAAGGACAAAGGTCCCCAA CGGTCAGCCAGTCCAGAAGTGGTTCCCAGGAGGCTGAGGAGAGTATCCACCCAAACACCCAAGAAAAGTA CAACTGTGCCACATCTCGCATCAACGAAGTTCAAGTCAACCTGTCCTTGTTAGGGGATGACCTGCTGCTT CCTGCTCAATCCACGCTTCAAACAAAGCATCCAGACATCTACTGCATTACAGACTTTGCGGAAGAATTAG CAGACACGGTCGTCTCCATGGCAACTGAAATTGCAGCGATTTGCCTTGACAACTCCAGTGGAAAACAACC CTGGTTTTGTGCATGGAAAAGAGGGAGTGAGTTTCTGATGACACCCAACGTACCCTGCCGATCCTTGAAG AGGAAGAAAGAGAGCCAGGGGAGCGGGACCGCTGTGAGGAAACACAAGCCTCCCCGGCTCAGTGAGATCA AGAGGAAGACGGACGAGCACCCTGAGCTTAAAGAAAAGCTGATGAACAGGGTTGTGGATGAGTCCATGAA CCTTGAAGATGTCCCAGATTCTGTCAATCTTTTTGCCAATGAAGTGGCAGCCAAGATCATGAACCTAACG GAGTTCTCTATGGTGGACGGCATGTGGCAGGCGCAGGGCTATCCCCGGAATCGGTTACTGAGTGGCGACA GGTGGAGCCGGCTGAAGGCCTCCAGCTGCGAAAGCATTCCTGAGGAAGACTCCGAGGCCAGGGCCTATGT CAACAGCCTGGGCTTAATGAGCACGCTGAGCCAGCCGGTCAGCAGGGCCAGCTCTGTCTCCAAGCAGTCG AGCTGTGAGAGCATCACCGATGAGTTTTCCAGGTTCATGGTGAACCAGATGGAAAATGAAGGGAGAGGAT TTGAGTTACTGCTGGATTACTATGCTGGCAAGAACGCCAGCAGCATTCTGAACTCAGCCATGCAACAGGC GTGCCGGAAAAGTGACCACCTCAGTGTGAGGCCTAGCTGTCCCTCTAAGCAGTCCAGCACAGAGAGCATC ACTGAGGAGTTCTACAGGTACATGCTGAGGGACATCGAAAGAGACAGCAGAGAAAGTGCCTCCTCCAGAC GGAGCAGCCAGATTTGGACAGCCGGCCTGCTGTCTCCTTCTCTGCGATCCCCAGTGTGCCACAGACAGTC GTCCATGCCAGACAGCAGATCCCCATGCTCCAGGCTGACAGTGAATGTGCCCATCAAAGCCAACTCTTTA GATGGCTTTGCTCAGAACTGCCCACAAGATTTCCTAAGCGTGCAGCCGGTCAGTAGCGCGTCCTCATCCG GTCTCTGCAAATCTGACTCTTGCTTGTATCGGAGAGGTGGGACTGACCACATCACCAACATGTTAATTCA TGAAACGTGGGCTAGCTCCATTGAGGCTCTCATGCGCAAGAACAAAATCATTGTGGATGATGCAGAGGAA GCTGACACTGAGCCTGTTTCTGGTGGCTCTCCCTCGCAAGCAGAGAAGTGTGCAAATAGATTAGCTGCGA GCAGGATGTGCAGTGGGCCAACTCTGCTTGTTCAGGAGTCTCTCGATTGCCCGAGGAAAGACTCTGTTAC CGAATGTAAACAGCCCCCAGTGTCATCTTTGAGCAAAACTGCTTCTCTTACAAACCACAGCCCTTTAGAT TCTAAAAAAGAAACTTCCTCGTGCCAGGACCCTGTACCAATAAACCACAAAAGGCGATCACTTTGCTCGA GGGAAGTGCCTTTGATTCAGATTGAAACAGATCAGAGAGAAGCCTGTGCTGGGGAACCTGAACCCTTCCT TTCCAAAAGCAGCCTCCTAGAGGAAGCAGAAGGGCATTCGAATGACAAAAACATCCCAGATGTGGTGAGA GGTGGAGACACAGCCGTGAGCGCTTGTCAAATCCATAGTGACAGCCTTGATACCAGAGATGTACCAGAGG CTGAAGCCTCCACAGAAGCCAGAGCCCCCGATGAGGCCCCCAACCCTCCAAGCAGCAGCGAGGAGAGCAC AGGCAGCTGGACCCAGCTTGCCAATGAGGAAGACAACCCAGATGACACAAGTAGCTTTCTCCAGCTCAGT GAGCGATCCATGAGTGAATTAGTAGAAGAAAAGAAGATTCTTAAAGGACAGTCAGAAAGCACAGAGGCAC CTGCATCTGGACCGCCTACGGGAACAGCCAGCCCCCAGAGGAGCCTGCTGGTGATCAACTTTGACCTGGA GCCAGAGTGTCCAGATGCCGAGCTCCGAGCCACTCTGCAGTGGATAGCTGCCTCTGAACTGGGGATTCCC ACCATCTACTTTAAGAAATCTCAGGAAAACAGAATTGAAAAGTTTCTAGATGTCGTGCAGCTGGTTCATC GGAAGTCCTGGAAAGTGGGTGATATCTTCCATGCAGTTGTCCAGTACTGCAAAATGCATGAGGAGCAGAA GGATGGGAGACTGAGTCTCTTTGACTGGCTCTTGGAACTGGGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC215958 representing NM_030623
Red=Cloning site Green=Tags(s) MDGNSLLSVPSNLESSRMYDVLEPQQGRGCGSSGSGPGNSITACKKVLRSNSLLESTDYWLQNQRMPCQI GFVEDKSENCASVCFVNLGVNKDECSTEHLQQKLVNVSPDLPKLISSMNVQQPKENEIVVLSGLASGNLQ ADFEVSQCPWLPDICLVQCARGNRPNSTNCIIFEINKFLIGLELVQERQLHLETNILKLEDDTNCSLSSI EEDFLTASEHLEEESEVDESRNDYENINVSANVLESKQLKGATQVEWNCNKEKWLYALEDKYINKYPTPL IKTERSPENLTKNTALQSLDPSAKPSQWKREAVGNGRQATHYYHSEAFKGQMEKSQALYIPKDAYFSMMD KDVPSACAVAEQRSNLNPGDHEDTRNALPPRQDGEVTTGKYATNLAESVLQDAFIRLSQSQSTLPQESAV SVSVGSSLLPSCYSTKDTVVSRSWNELPKIVVVQSPDGSDAAPQPGISSWPEMEVSVETSSIPSGENSSR QPQSALEVALACAATVIGTISSPQATERLKMEQVVSNFPPGSSGALQTQAPQGLKEPSINEYSFPSALCG MTQVASAVAVCGLGEREEVTCSVAPSGSLPPAAEASEAMPPLCGLASMELGKEAIAKGLLKEAALVLTRP NTYSSIGDFLDSMNRRIMETASKSQTLCSENVVRNELAHTLSNVILRHSIDEVHHKNMIIDPNDNRHSSE ILDTLMESTNQLLLDVICFTFKKMSHIVRLGECPAVLSKETIRRRETEPSCQPSDPGASQAWTKATESSS SSPLSNSHNTSLVINNLVDGMYSKQDKGGVRPGLFKNPTLQSQLSRSHRVPDSSTATTSSKEIYLKGIAG EDTKSPHHSENECRASSEGQRSPTVSQSRSGSQEAEESIHPNTQEKYNCATSRINEVQVNLSLLGDDLLL PAQSTLQTKHPDIYCITDFAEELADTVVSMATEIAAICLDNSSGKQPWFCAWKRGSEFLMTPNVPCRSLK RKKESQGSGTAVRKHKPPRLSEIKRKTDEHPELKEKLMNRVVDESMNLEDVPDSVNLFANEVAAKIMNLT EFSMVDGMWQAQGYPRNRLLSGDRWSRLKASSCESIPEEDSEARAYVNSLGLMSTLSQPVSRASSVSKQS SCESITDEFSRFMVNQMENEGRGFELLLDYYAGKNASSILNSAMQQACRKSDHLSVRPSCPSKQSSTESI TEEFYRYMLRDIERDSRESASSRRSSQIWTAGLLSPSLRSPVCHRQSSMPDSRSPCSRLTVNVPIKANSL DGFAQNCPQDFLSVQPVSSASSSGLCKSDSCLYRRGGTDHITNMLIHETWASSIEALMRKNKIIVDDAEE ADTEPVSGGSPSQAEKCANRLAASRMCSGPTLLVQESLDCPRKDSVTECKQPPVSSLSKTASLTNHSPLD SKKETSSCQDPVPINHKRRSLCSREVPLIQIETDQREACAGEPEPFLSKSSLLEEAEGHSNDKNIPDVVR GGDTAVSACQIHSDSLDTRDVPEAEASTEARAPDEAPNPPSSSEESTGSWTQLANEEDNPDDTSSFLQLS ERSMSELVEEKKILKGQSESTEAPASGPPTGTASPQRSLLVINFDLEPECPDAELRATLQWIAASELGIP TIYFKKSQENRIEKFLDVVQLVHRKSWKVGDIFHAVVQYCKMHEEQKDGRLSLFDWLLELG TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_030623 |
ORF Size | 5013 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_030623.2 |
RefSeq Size | 6825 bp |
RefSeq ORF | 5016 bp |
Locus ID | 80309 |
UniProt ID | Q2M3C7 |
Protein Families | Druggable Genome |
MW | 183.2 kDa |
Gene Summary | Anchoring protein that binds preferentially to the type I regulatory subunit of c-AMP-dependent protein kinase (PKA type I) and targets it to distinct subcellular compartments. May act as a converging factor linking cAMP and sphingosine signaling pathways. Plays a regulatory role in the modulation of SPHK1.[UniProtKB/Swiss-Prot Function] |
Citations (1)
The use of this cDNA Clones has been cited in the following citations: |
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Repression of sphingosine kinase (SK)-interacting protein (SKIP) in acute myeloid leukemia diminishes SK activity and its re-expression restores SK function
,Ghazaly, EA;Miraki-Moud, F;Smith, P;Gnanaranjan, C;Koniali, L;Oke, A;Saied, MH;Petty, R;Matthews, J;Stronge, R;Joel, SP;Young, BD;Gribben, J;Taussig, DC;,
J. Biol. Chem.
,PubMed ID 32161116
[SPHKAP]
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