SPHKAP (NM_030623) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	SKIP
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC305270 representing NM_030623. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGATGGCAACTCCCTGCTCTCGGTACCAAGCAACTTGGAGTCATCACGGATGTATGACGTTTTGGAA CCGCAGCAGGGCAGAGGCTGTGGCAGCTCAGGAAGCGGCCCGGGGAACTCCATCACAGCCTGTAAGAAG GTTCTTCGCAGCAATAGCCTGCTGGAGTCAACAGACTACTGGTTGCAGAATCAGAGGATGCCCTGCCAA ATTGGTTTTGTAGAAGACAAGTCTGAAAACTGTGCTTCTGTCTGCTTTGTGAATCTTGATGTGAACAAG GATGAATGCAGCACAGAGCACCTGCAACAGAAACTGGTCAACGTTTCACCAGATCTTCCAAAACTTATC AGTTCCATGAATGTCCAACAACCAAAAGAAAATGAAATTGTTGTCCTAAGTGGGTTAGCCTCTGGAAAT CTCCAGGCAGATTTTGAAGTTTCACAGTGCCCTTGGCTGCCAGATATCTGCTTGGTCCAATGTGCAAGA GGGAACAGACCAAACAGTACCAACTGCATCATCTTTGAAATCAACAAATTTCTGATTGGTCTGGAACTG GTGCAGGAGCGACAGCTCCACCTGGAAACAAACATCTTGAAACTGGAGGATGACACGAACTGTTCCTTA TCTTCAATCGAGGAAGACTTTCTCACCGCTTCTGAGCACTTGGAGGAGGAAAGCGAGGTGGATGAATCT AGGAACGATTATGAAAATATAAATGTCTCAGCCAATGTTTTGGAAAGTAAACAGCTAAAGGGAGCCACC CAGGTGGAATGGAATTGCAACAAGGAAAAGTGGCTTTATGCTTTGGAAGACAAATACATCAACAAATAT CCCACACCATTGATTAAAACAGAACGATCTCCAGAAAACCTAACAAAGAACACAGCCTTGCAGAGTCTA GATCCCTCAGCCAAGCCATCACAGTGGAAAAGAGAAGCTGTGGGGAATGGGAGACAAGCCACACATTAT TATCATTCAGAAGCTTTTAAAGGTCAAATGGAAAAATCACAGGCACTGTATATTCCAAAAGATGCTTAT TTCTCCATGATGGATAAAGATGTACCTTCTGCATGTGCTGTGGCAGAGCAGAGAAGCAACCTAAACCCA GGAGACCATGAAGACACAAGAAACGCTCTCCCTCCTAGACAAGATGGAGAAGTCACCACTGGCAAGTAT GCTACAAATTTAGCAGAATCCGTGCTGCAGGATGCATTTATTAGATTATCTCAATCTCAGTCCACATTA CCCCAGGAATCTGCAGTCAGTGTTTCTGTAGGAAGTTCTCTGCTTCCCAGTTGCTATTCCACAAAAGAT ACAGTGGTTTCTCGGTCATGGAATGAGCTCCCCAAAATCGTCGTTGTTCAGAGTCCAGATGGCAGTGAT GCTGCCCCACAGCCAGGCATCTCCTCCTGGCCTGAGATGGAAGTCTCTGTTGAAACCTCAAGCATCCTC TCTGGAGAGAACTCCAGCAGACAACCCCAGAGTGCTCTAGAAGTGGCGTTAGCTTGTGCAGCCACTGTG ATTGGAACTATTTCCAGTCCACAGGCCACAGAAAGACTCAAAATGGAGCAAGTGGTCTCGAACTTTCCC CCAGGGAGCAGTGGTGCACTGCAAACTCAAGCACCCCAAGGACTCAAGGAACCTTCCATCAATGAGTAC TCCTTTCCATCTGCTTTGTGTGGCATGACTCAGGTGGCCAGTGCCGTGGCTGTCTGTGGTCTGGGTGAA AGAGAAGAGGTGACATGCTCAGTGGCTCCAAGTGGTAGCCTCCCGCCTGCAGCTGAGGCTTCTGAAGCC ATGCCCCCACTTTGTGGTTTAGCAAGCATGGAGCTTGGCAAGGAAGCCATTGCCAAGGGATTGCTCAAG GAGGCTGCTCTGGTTTTAACAAGGCCTAATACCTACAGCAGCATTGGAGACTTTCTGGACTCCATGAAC AGGAGAATCATGGAAACTGCTTCAAAGTCTCAGACCCTGTGCTCAGAAAATGTCGTCAGGAATGAACTG GCACATACCCTGTCCAATGTTATCCTGAGGCATTCCATTGATGAAGTTCACCACAAAAATATGATAATC GACCCCAATGACAACAGGCATTCATCTGAAATTCTGGACACCTTAATGGAAAGTACAAATCAACTGCTT TTAGATGTGATATGCTTCACGTTCAAGAAGATGAGTCATATTGTACGGCTTGGTGAATGTCCTGCTGTC CTTTCTAAGGAGACCATCAGAAGGAGGGAGACAGAACCAAGCTGCCAGCCATCTGATCCGGGTGCTAGT CAAGCTTGGACAAAAGCCACTGAATCCTCCAGCAGCTCTCCACTTAGCAATTCACACAACACGAGTCTT GTCATCAACAATCTTGTGGATGGCATGTATTCAAAACAAGACAAGGGTGGAGTGAGGCCAGGCCTCTTC AAGAACCCCACGCTGCAGTCACAATTATCACGTAGTCACAGAGTGCCCGATTCTTCAACTGCTACAACA TCCTCCAAGGAAATATATCTGAAAGGAATAGCAGGAGAGGATACAAAAAGCCCTCATCACAGTGAGAAT GAATGCAGAGCCTCTTCCGAAGGACAAAGGTCCCCAACGGTCAGCCAGTCCAGAAGTGGTTCCCAGGAG GCTGAGGAGAGTATCCACCCAAACACCCAAGAAAAGTACAACTGTGCCACATCTCGCATCAACGAAGTT CAAGTCAACCTGTCCTTGTTAGGGGATGACCTGCTGCTTCCTGCTCAATCCACGCTTCAAACAAAGCAT CCAGACATCTACTGCATTACAGACTTTGCGGAAGAATTAGCAGACACGGTCGTCTCCATGGCAACTGAA ATTGCAGCGATTTGCCTTGACAACTCCAGTGGAAAACAACCCTGGTTTTGTGCATGGAAAAGAGGGAGT GAGTTTCTGATGACACCCAACGTACCCTGCCGATCCTTGAAGAGGAAGAAAGAGAGCCAGGGGAGCGGG ACCGCTGTGAGGAAACACAAGCCTCCCCGGCTCAGTGAGATCAAGAGGAAGACGGACGAGCACCCTGAG CTTAAAGAAAAGCTGATGAACAGGGTTGTGGATGAGTCCATGAACCTTGAAGATGTCCCAGATTCTGTC AATCTTTTTGCCAATGAAGTGGCAGCCAAGATCATGAACCTAACGGAGTTCTCTATGGTGGACGGCATG TGGCAGGCGCAGGGCTATCCCCGGAATCGGTTACTGAGTGGCGACAGGTGGAGCCGGCTGAAGGCCTCC AGCTGCGAAAGCATTCCTGAGGAAGACTCCGAGGCCAGGGCCTATGTCAACAGCCTGGGCTTAATGAGC ACGCTGAGCCAGCCGGTCAGCAGGGCCAGCTCTGTCTCCAAGCAGTCGAGCTGTGAGAGCATCACCGAT GAGTTTTCCAGGTTCATGGTGAACCAGATGGAAAATGAAGGGAGAGGATTTGAGTTACTGCTGGATTAC TATGCTGGCAAGAACGCCAGCAGCATTCTGAACTCAGCCATGCAACAGGCGTGCCGGAAAAGTGACCAC CTCAGTGTGAGGCCTAGCTGTCCCTCTAAGCAGTCCAGCACAGAGAGCATCACTGAGGAGTTCTACAGG TACATGCTGAGGGACATCGAAAGAGACAGCAGAGAAAGTGCCTCCTCCAGACGGAGCAGCCAGGATTGG ACAGCCGGCCTGCTGTCTCCTTCTCTGCGATCCCCAGTGTGCCACAGACAGTCGTCCATGCCAGACAGC AGATCCCCATGCTCCAGGCTGACAGTGAATGTGCCCATCAAAGCCAACTCTTTAGATGGCTTTGCTCAG AACTGCCCACAAGATTTCCTAAGCGTGCAGCCGGTCAGTAGCGCGTCCTCATCCGGTCTCTGCAAATCT GACTCTTGCTTGTATCGGAGAGGTGGGACTGACCACATCACCAACATGTTAATTCATGAAACGTGGGCT AGCTCCATTGAGGCTCTCATGCGCAAGAACAAAATCATTGTGGATGATGCAGAGGAAGCTGACACTGAG CCTGTTTCTGGTGGCTCTCCCTCGCAAGCAGAGAAGTGTGCAAATAGATTAGCTGCGAGCAGGATGTGC AGTGGGCCAACTCTGCTTGTTCAGGAGTCTCTCGATTGCCCGAGGAAAGACTCTGTTACCGAATGTAAA CAGCCCCCAGTGTCATCTTTGAGCAAAACTGCTTCTCTTACAAACCACAGCCCTTTAGATTCTAAAAAA GAAACTTCCTCGTGCCAGGACCCTGTACCAATAAACCACAAAAGGCGATCACTTTGCTCGAGGGAAGTG CCTTTGATTCAGATTGAAACAGATCAGAGAGAAGCCTGTGCTGGGGAACCTGAACCCTTCCTTTCCAAA AGCAGCCTCCTAGAGGAAGCAGAAGGGCATTCGAATGACAAAAACATCCCAGATGTGGTGAGAGGTGGA GACACAGCCGTGAGCGCTTGTCAAATCCATAGTGACAGCCTTGATACCAGAGATGTACCAGAGGCTGAA GCCTCCACAGAAGCCAGAGCCCCCGATGAGGCCCCCAACCCTCCAAGCAGCAGCGAGGAGAGCACAGGC AGCTGGACCCAGCTTGCCAATGAGGAAGACAACCCAGATGACACAAGTAGCTTTCTCCAGCTCAGTGAG CGATCCATGAGTGAATTAGTAGAAGAAAAGAAGATTCTTAAAGGACAGTCAGAAAGCACAGAGGCACCT GCATCTGGACCGCCTACGGGAACAGCCAGCCCCCAGAGGAGCCTGCTGGTGATCAACTTTGACCTGGAG CCAGAGTGTCCAGATGCCGAGCTCCGAGCCACTCTGCAGTGGATAGCTGCCTCTGAACTGGGGATTCCC ACCATCTACTTTAAGAAATCTCAGGAAAACAGAATTGAAAAGTTTCTAGATGTCGTGCAGCTGGTTCAT CGGAAGTCCTGGAAAGTGGGTGATATCTTCCATGCAGTTGTCCAGTACTGCAAAATGCATGAGGAGCAG AAGGATGGGAGACTGAGTCTCTTTGACTGGCTCTTGGAACTGGGATAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_030623
Insert Size	5016 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_030623.3
RefSeq Size	6826 bp
RefSeq ORF	5016 bp
Locus ID	80309
UniProt ID	Q2M3C7
Protein Families	Druggable Genome
MW	183.4 kDa
Gene Summary	Anchoring protein that binds preferentially to the type I regulatory subunit of c-AMP-dependent protein kinase (PKA type I) and targets it to distinct subcellular compartments. May act as a converging factor linking cAMP and sphingosine signaling pathways. Plays a regulatory role in the modulation of SPHK1.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) lacks an in-frame exon in the 3' coding region, compared to variant 1, and encodes a shorter isoform (2) than isoform 1.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.