United States
Germany
Japan
United Kingdom
China
SEC14 like protein 2 (SEC14L2) (NM_012429) Human Tagged ORF Clone
CAT＃: RC215994
SEC14L2 (Myc-DDK-tagged)-Human SEC14-like 2 (S. cerevisiae) (SEC14L2), transcript variant 1
ORF Plasmid: tGFP
"NM_012429" in other vectors (6)
Need custom modification / cloning service?
Get a free quote
CNY 5,488.00
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | C22orf6; SPF; TAP; TAP1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC215994 representing NM_012429
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCGGCAGAGTCGGCGATCTGAGCCCCAGGCAGAAGGAGGCATTGGCCAAGTTTCGGGAGAATGTCC AGGATGTGCTGCCGGCCCTGCCGAATCCAGATGACTATTTTCTCCTGCGTTGGCTCCGAGCCAGAAGCTT CGACCTGCAGAAGTCGGAGGCCATGCTCCGGAAGCATGTGGAGTTCCGAAAGCAAAAGGACATTGACAAC ATCATTAGCTGGCAGCCTCCAGAGGTGATCCAACAGTATCTGTCAGGGGGTATGTGTGGCTATGACCTGG ATGGCTGCCCAGTCTGGTACGACATAATTGGACCTCTGGATGCCAAGGGTCTGCTGTTCTCAGCCTCCAA ACAGGACCTGCTGAGGACCAAGATGCGGGAGTGTGAGCTGCTTCTGCAAGAGTGTGCCCACCAGACCACA AAGTTGGGGAGGAAGGTGGAGACCATCACCATAATTTATGACTGCGAGGGGCTTGGCCTCAAGCATCTCT GGAAGCCTGCTGTGGAGGCCTATGGAGAGTTTCTCTGCATGTTTGAGGAAAATTATCCCGAAACACTGAA GCGTCTTTTTGTTGTTAAAGCCCCCAAACTGTTTCCTGTGGCCTATAACCTCATCAAACCCTTCCTGAGT GAGGACACTCGTAAGAAGATCATGGTCCTGGGAGCAAATTGGAAGGAGGTTTTACTGAAACATATCAGCC CTGACCAGGTGCCTGTGGAGTATGGGGGCACCATGACTGACCCTGATGGAAACCCCAAGTGCAAATCCAA GATCAACTACGGGGGTGACATCCCCAGGAAGTATTATGTGCGAGACCAGGTGAAACAGCAGTATGAACAC AGCGTGCAGATTTCCCGTGGCTCCTCCCACCAAGTGGAGTATGAGATCCTCTTCCCTGGCTGTGTCCTCA GGTGGCAGTTTATGTCAGATGGAGCGGATGTTGGTTTTGGGATTTTCCTGAAGACCAAGATGGGAGAGAG GCAGCGGGCAGGGGAGATGACAGAGGTGCTGCCCAACCAGAGGTACAACTCCCACCTGGTCCCTGAAGAT GGGACCCTCACCTGCAGTGATCCTGGCATCTATGTCCTGCGGTTTGACAACACCTACAGCTTCATTCATG CCAAGAAGGTCAATTTCACTGTGGAGGTCCTGCTTCCAGACAAAGCCTCAGAAGAGAAGATGAAACAGCT GGGGGCAGGCACCCCGAAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC215994 representing NM_012429
Red=Cloning site Green=Tags(s) MSGRVGDLSPRQKEALAKFRENVQDVLPALPNPDDYFLLRWLRARSFDLQKSEAMLRKHVEFRKQKDIDN IISWQPPEVIQQYLSGGMCGYDLDGCPVWYDIIGPLDAKGLLFSASKQDLLRTKMRECELLLQECAHQTT KLGRKVETITIIYDCEGLGLKHLWKPAVEAYGEFLCMFEENYPETLKRLFVVKAPKLFPVAYNLIKPFLS EDTRKKIMVLGANWKEVLLKHISPDQVPVEYGGTMTDPDGNPKCKSKINYGGDIPRKYYVRDQVKQQYEH SVQISRGSSHQVEYEILFPGCVLRWQFMSDGADVGFGIFLKTKMGERQRAGEMTEVLPNQRYNSHLVPED GTLTCSDPGIYVLRFDNTYSFIHAKKVNFTVEVLLPDKASEEKMKQLGAGTPK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_012429 |
| ORF Size | 1209 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_012429.5 |
| RefSeq Size | 2818 bp |
| RefSeq ORF | 1212 bp |
| Locus ID | 23541 |
| UniProt ID | O76054 |
| Domains | SEC14 |
| Protein Families | Transcription Factors |
| MW | 46 kDa |
| Gene Summary | This gene encodes a cytosolic protein which belongs to a family of lipid-binding proteins including Sec14p, alpha-tocopherol transfer protein, and cellular retinol-binding protein. The encoded protein stimulates squalene monooxygenase which is a downstream enzyme in the cholesterol biosynthetic pathway. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Oct 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC215994L1 | Lenti ORF clone of Human SEC14-like 2 (S. cerevisiae) (SEC14L2), transcript variant 1, Myc-DDK-tagged |
CNY 7,888.00 |
|
| RC215994L2 | Lenti ORF clone of Human SEC14-like 2 (S. cerevisiae) (SEC14L2), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RC215994L3 | Lenti ORF clone of Human SEC14-like 2 (S. cerevisiae) (SEC14L2), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC215994L4 | Lenti ORF clone of Human SEC14-like 2 (S. cerevisiae) (SEC14L2), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RG215994 | SEC14L2 (tGFP-tagged) - Human SEC14-like 2 (S. cerevisiae) (SEC14L2), transcript variant 1 |
CNY 7,088.00 |
|
| SC115384 | SEC14L2 (untagged)-Human SEC14-like 2 (S. cerevisiae) (SEC14L2), transcript variant 1 |
CNY 3,656.00 |
其它SEC14 like protein 2产品
