Myeloperoxidase (MPO) (NM_000250) Human Tagged Lenti ORF Clone

CAT#: RC216029L1

  • LentiORF®

Lenti ORF clone of Human myeloperoxidase (MPO), nuclear gene encoding mitochondrial protein, Myc-DDK-tagged



  "NM_000250" in other vectors (6)

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CNY 7,856.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
Lenti-vpak packaging kit - packaging plasmids and transfection reagent
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Forward primer to sequence targets cloned in expression vectors.
    • 1 nmol

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Reverse primer to sequence targets cloned in expression vectors.
    • 1 nmol

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Myeloperoxidase (MPO) Rabbit polyclonal Antibody
    • 100 ul

CNY 1,999.00
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DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
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Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Vector pLenti-C-Myc-DDK
E. coli Selection Chloramphenicol (34 ug/mL)
Mammalian Cell Selection None
Sequence Data
The ORF insert of this clone is exactly the same as(RC216029).
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_000250
ORF Size 2235 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_000250.1
RefSeq Size 3215 bp
RefSeq ORF 2238 bp
Locus ID 4353
UniProt ID P05164
Domains An_peroxidase
Protein Families Druggable Genome
MW 83.7 kDa
Gene Summary Myeloperoxidase (MPO) is a heme protein synthesized during myeloid differentiation that constitutes the major component of neutrophil azurophilic granules. Produced as a single chain precursor, myeloperoxidase is subsequently cleaved into a light and heavy chain. The mature myeloperoxidase is a tetramer composed of 2 light chains and 2 heavy chains. This enzyme produces hypohalous acids central to the microbicidal activity of neutrophils. [provided by RefSeq, Nov 2014]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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