Myeloperoxidase (MPO) (NM_000250) Human Untagged Clone

CAT#: SC119994

MPO (untagged)-Human myeloperoxidase (MPO), nuclear gene encoding mitochondrial protein



  "NM_000250" in other vectors (6)

CNY 5,464.00

CNY 7,220.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
Myeloperoxidase (MPO) Rabbit polyclonal Antibody
    • 100 ul

CNY 1,999.00
CNY 3,280.00

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TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC119994 sequence for NM_000250 edited (data generated by NextGen Sequencing)
ATGGGGGTTCCCTTCTTCTCTTCTCTCAGATGCATGGTGGACTTAGGACCTTGCTGGGCT
GGGGGTCTCACTGCAGAGATGAAGCTGCTTCTGGCCCTAGCAGGGCTCCTGGCCATTCTG
GCCACGCCCCAGCCCTCTGAAGGTGCTGCTCCAGCTGTCCTGGGGGAGGTGGACACCTCG
TTGGTGCTGAGCTCCATGGAGGAGGCCAAGCAGCTGGTGGACAAGGCCTACAAGGAGCGG
CGGGAAAGCATCAAGCAGCGGCTTCGCAGCGGCTCAGCCAGCCCCATGGAACTCCTATCC
TACTTCAAGCAGCCGGTGGCAGCCACCAGGACGGCGGTGAGGGCCGCTGACTACCTGCAC
GTGGCTCTAGACCTGCTGGAGAGGAAGCTGCGGTCCCTGTGGCGAAGGCCATTCAATGTC
ACTGATGTGCTGACGCCCGCCCAGCTGAATGTGTTGTCCAAGTCAAGCGGCTGCGCCTAC
CAGGACGTGGGGGTGACTTGCCCGGAGCAGGACAAATACCGCACCATCACCGGGATGTGC
AACAACAGACGCAGCCCCACGCTGGGGGCCTCCAACCGTGCCTTTGTGCGCTGGCTGCCG
GCGGAGTATGAGGACGGCTTCTCTCTTCCCTACGGCTGGACGCCCGGGGTCAAGCGCAAC
GGCTTCCCGGTGGCTCTGGCTCGCGCGGTCTCCAACGAGATCGTGCGCTTCCCCACTGAT
CAGCTGACTCCGGACCAGGAGCGCTCACTCATGTTCATGCAATGGGGCCAGCTGTTGGAC
CACGACCTCGACTTCACCCCTGAGCCGGCCGCCCGGGCCTCCTTCGTCACTGGCGTCAAC
TGCGAGACCAGCTGCGTTCAGCAGCCGCCCTGCTTCCCGCTCAAGATCCCGCCCAATGAC
CCCCGCATCAAGAACCAAGCCGACTGCATCCCGTTCTTCCGCTCCTGCCCGGCTTGCCCC
GGGAGCAACATCACCATCCGCAACCAGATCAACGCGCTCACTTCCTTCGTGGACGCCAGC
ATGGTGTACGGCAGCGAGGAGCCCCTGGCCAGGAACCTGCGCAACATGTCCAACCAGCTG
GGGCTGCTGGCCGTCAACCAGCGCTTCCAAGACAACGGCCGGGCCCTGCTGCCCTTTGAC
AACCTGCACGATGACCCCTGTCTCCTCACCAACCGCTCAGCGCGCATCCCCTGCTTCCTG
GCAGGGGACACCCGTTCCAGTGAGATGCCCGAGCTCACCTCCATGCACACCCTCTTACTT
CGGGAGCACAACCGGCTGGCCACAGAGCTCAAGAGCCTGAACCCTAGGTGGGATGGGGAG
AGGCTCTACCAGGAAGCCCGGAAGATCGTGGGGGCCATGGTCCAGATCATCACTTACCGG
GACTACCTGCCCCTGGTGCTGGGGCCAACGGCCATGAGGAAGTACCTGCCCACGTACCGT
TCCTACAATGACTCAGTGGACCCACGCATCGCCAACGTCTTCACCAATGCCTTCCGCTAC
GGCCACACCCTCATCCAACCCTTCATGTTCCGCCTGGACAATCGGTACCAGCCCATGGAA
CCCAACCCCCGTGTCCCCCTCAGCAGGGTCTTTTTTGCCTCCTGGAGGGTCGTGCTGGAA
GGTGGCATTGACCCCATCCTCCGGGGCCTCATGGCCACCCCTGCCAAGCTGAATCGTCAG
AACCAAATTGCAGTGGATGAGATCCGGGAGCGATTGTTTGAGCAGGTCATGAGGATTGGG
CTGGACCTGCCTGCTCTGAACATGCAGCGCAGCAGGGACCACGGCCTCCCAGGATACAAT
GCCTGGAGGCGCTTCTGTGGGCTCCCGCAGCCTGAAACTGTGGGCCAGCTGGGCACGGTG
CTGAGGAACCTGAAATTGGCGAGGAAACTGATGGAGCAGTATGGCACGCCCAACAACATC
GACATCTGGATGGGCGGCGTGTCCGAGCCTCTGAAGCGCAAAGGCCGCGTGGGCCCACTC
CTCGCCTGCATCATCGGTACCCAGTTCAGGAAGCTCCGGGATGGTGATCGGTTTTGGTGG
GAGAACGAGGGTGTGTTCAGCATGCAGCAGCGACAGGCCCTGGCCCAGATCTCATTGCCC
CGGATCATCTGCGACAACACAGGCATCACCACCGTGTCTAAGAACAACATCTTCATGTCC
AACTCATATCCCCGGGACTTTGTCAACTGCAGTACACTTCCTGCATTGAACCTGGCTTCC
TGGAGGGAAGCCTCCTAG

Clone variation with respect to NM_000250.1
>OriGene 5' read for NM_000250 unedited
TAAGTTTAAATATTTGTATACGACTCATATAGGCGGCCGCGNAATTCGCACCAGGTCCTT
GGAAGCTGGATGACAGCAGCTGGCAAGGGGATAAGAGAGCAGTGAGCCCCTCCCTCAAGA
GGTCTGGCTTTATCCATAGACAGGGCCCTCTGAGGTGGGGCTGAGGTACAAAGGGGGATT
GAGCAGCCCAGGAGAAGAGAGATGGGGGTTCCCTTCTTCTCTTCTCTCAGATGCATGGTG
GACTTAGGACCTTGCTGGGCTGGGGGTCTCACTGCAGAGATGAAGCTGCTTCTGGCCCTA
GCAGGGCTCCTGGCCATTCTGGCCACGCCCCAGCCCTCTGAAGGTGCTGCTCCAGCTGTC
CTGGGGGAGGTGGACACCTCGTTGGTGCTGAGCTCCATGGAGGAGGCCAAGCAGCTGGTG
GACAAGGCCTACAAGGAGCGGCGGGAAAGCATCAAGCAGCGGCTTCGCAGCGGCTCAGCC
AGCCCCATGGAACTCCTATCCTACTTCAAGCAGCCGGTGGCAGCCACCAGGACGGCGGTG
AGGGCCGCTGACTACCTGCACGTGGCTCTAGACCTGCTGGAGAGGAAGCTGCGGTCCCTG
TGGCGAAGGCCATTCAATGTCACTGATGTGCTGACGCCCGCCCAGCTGAATGTGTTGTCC
AAGTCAAGCGGCTGCGCCTACCAGGACGTGGGGGTGACTTGCCCGGAGCANGACAAATAC
CGCACCATCACCGGNGATGTGCAACAACAGACGCAGCCCCACGCTGGGGGCCTCCAACCG
TGCCTTTGTGCGCTGGCTGCNCGCNGAGTATGANGACGGCTTCTCTCTTCCCTACNGCTG
GACGCCCGGGGTCAAGCGCAAACGCTTACCCGTGGCTCTGGCTCGCGCGGTCTCCACCNA
NATCGTGC
>OriGene 3' read for NM_000250 unedited
AAAAGGGGAAAANNACANNNCNNCTTTCCCCCCCTNTTTTTACTTAGNNACGCGGNCCGC
ATNCTANGATCGAGTTTTTTTTTTTTTTTTTTTCAGCAAACACACACGCATGAAAAAGCC
AATTTATATACTTCGCACATACATGAGCACACAAATGAACGTCTAGTCACTCATTTTCTC
AGCTGCACCCAAACAGGGCTGGGCTCATCTAGGGCAAGGAGATCTCCGTGGTTCCAACTG
GCCAGCCCAGATATACCCCTCACTGCTGCACCCCCTTACCTGGCCTCTAGGAGGCTTCCC
TCCAGGAAGCCAGGTTCAATGCAGGAAGTGTACTGCAGTTGACAAAGTCCCGGGGATATG
AGTTGGACATGAAGATGTTGTTCTTAGACACGGTGGTGATGCCTGTGTTGTCGCAGATGA
TCCGGGGCAATGAGATCTGGGCCAGGGCCTGTCGCTGCTGCATGCTGAACACACCCTCGT
TCTCCCACCAAAACCGATCACCATCCCGGAGCTTCCTGAACTGGGTACCGATGATGCAGG
CGAGGAGTGGGCCCACGCGGCCTTTGCGCTTCAGAGGCTCGGACACGCCGCCCATCCAGA
TGTCGATGTTGTTGGGCGTGCCATACTGCTCCATCAGTTTCCTCGCCAATTTCAGGTTCC
TCAGCACCGTGCCCAGCTGGCCCACAGTTTCAGGCTGCGGGAGCCCACAGAAGCGCCTCC
AGGCATTGTATCCTGGGAGGCCGTGGTCCCTGCTGCGCTGCATGTTCAAAGCAAGCAGGT
CCAGCCCAATCCTATGACCTGCTCAACAATCGCTCCCGGACTCATCCCTGCAATTTGGTT
CTGACCATTCACCTGGCAGGGTGGGCATGAGCCCCCGAAGAAGGGTCAATGCCACCTTCA
GACGACCCTCAGAAGCAAAAAAACCCTCTTAGGGGAACACGGGGTTGGGTCCATGGCTTG
TACCCATGTCCAGCCGCACATGAGGTTGGA
Restriction Sites NotI-NotI     
ACCN NM_000250
Insert Size 2560 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_000250.1, NP_000241.1
RefSeq Size 3215 bp
RefSeq ORF 2238 bp
Locus ID 4353
UniProt ID P05164
Domains An_peroxidase
Protein Families Druggable Genome
Gene Summary Myeloperoxidase (MPO) is a heme protein synthesized during myeloid differentiation that constitutes the major component of neutrophil azurophilic granules. Produced as a single chain precursor, myeloperoxidase is subsequently cleaved into a light and heavy chain. The mature myeloperoxidase is a tetramer composed of 2 light chains and 2 heavy chains. This enzyme produces hypohalous acids central to the microbicidal activity of neutrophils. [provided by RefSeq, Nov 2014]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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