GARNL1 (RALGAPA1) (NM_014990) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	GARNL1; GRIPE; NEDHRIT; p240; RalGAPalpha1; TULIP1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC216040 representing NM_014990 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTCTCCAAGAAGCCGCACGGGGACGTGAAGAAGTCCACCCAGAAGGTGCTAGACACCAAGAAGGACG CACTGACTCGCCTCAAGCACCTGCGCATCGTCATCGAGAATGCAGAATCTATTGATCTTAAACAGTTTTT CGACCAACATTTTTCACATATATACTATGTGTTCTTTGAAAATTTTGTGACTATTGAAGCTAGTCTTAAA CAGAAAGGTCACAAGTCTCAAAGAGAGGAATTGGATGCTATACTTTTTATTTTTGAGAAAATTTTACAAC TTCTTCCAGAAAGAATTCATCAGCGATGGCAGTTTCATAGTATTGGATTGATTTTAAAGAAGCTACTTCA CACAGGAAACTCCTTAAAGATTAGGCGGGAAGGTGTTCGTCTTTTCTTACTATGGTTGCAAGCTCTTCAG AATAACTGTAGCAAAGAACAGCTCTGGATGTTTTCATGCTTAATCCCTGGATTTTCAGCACCACAGTCTG AACATGGACCTCGAACTTTAGATAATCTCATTAATCCTCCACTCAACCTTCAAGAAACTCAAGTCACTAT AGAAGAAATCACTCCTCTTGTCCCCCCACAATCAGGAGATAAAGGGCAAGAAGATCTCACAAGCTATTTT CTTGAAGCACTTCTAAAATACATAGTCATTCAGGTAAAAAGTTTAGAATGGAAGAACAAAGAAAACCAAG AAAGGGGATTTTCATTTTTGTTTTCACATTTTAAGAAATATTACTTGCCTTATATTTTTCCAAACATCTG TAAGGAAAACAGTTTATATCATCCTATACTTGACATCCCGCAGATGAGACCAAAGCCACATTATGTCGTG ATAAAGAAAGATGCTGAAACCAATGAAGCAATCTATTGTACAAAGGAGCCTTTCATTAAGGCTCGTGTTA TTGTCATTCGTTGGCTGGTTTCTTTCTGGCTGGAGCCAAAACCACATACAGGACCTCATATTCCTGGGAT GGAAGGTGAAGTCTTGCCAAAGAATATTCAGAGAGCAGCTGCTAGTTTAGTATCCAGAGAAGAAAGCAAA AATGATAATGCTGATAAAACAGACAGAACTACAGAACCCGAACAGTCTCATTCCAATACAAGCACTCTCA CGGAGCGAGAACCTAGCTCATCTAGTCTCTGTAGTATTGATGAAGAACATCTCACAGACATTGAAATAGT TCGCAGAGTTTTTTCTTCTAAAAGGAGTAATGTAAACTTTGTGACAGAGATATTTCGTCAGGCATTTTTA TTACCAATTTGTGAAGCAGCAGCTATGAGAAAAGTGGTAAAAGTATATCAAGAATGGATCCAACAAGAGG AAAAACCTTTGTTCATGCAAGAGCCTGAAGAAATTGTGATCACTTCTTCAGACCTCCCTTGCATTGAAAA TGTCACAGACCATGATATTTCAATGGAAGAAGGAGAAAAAAGAGAAGAGGAAAATGGGACCAATACTGCT GATCATGTTCGAAATTCCAGTTGGGCAAAAAACGGCTCCTACCAAGGTGCTCTTCATAACGCCTCTGAAG AAGCCACAGAACAAAACATACGAGCTGGTACCCAGGCAGTTTTGCAGGTGTTTATTATAAACTCATCAAA TATATTTCTTCTTGAACCTGCAAATGAAATAAAAAATCTTCTGGATGAACACACAGATATGTGTAAACGC ATTCTTAACATTTATCGGTACATGGTTGTACAAGTATCAATGGACAAAAAGACTTGGGAACAGATGCTGC TTGTGTTGCTCAGAGTCACGGAATCTGTACTGAAGATGCCATCACAAGCTTTTCTACAGTTCCAAGGGAA AAAAAATATGACCTTGGCAGGTCGACTTGCAGGACCACTTTTCCAGACCCTTATAGTTGCCTGGATCAAA GCAAACCTAAATGTGTACATCTCCCGAGAACTTTGGGATGACTTACTGTCAGTATTGTCATCGCTGACCT ATTGGGAAGAGTTGGCCACTGAGTGGTCACTGACTATGGAGACATTAACTAAAGTTTTAGCTAGGAATTT ATATAGTTTGGATCTCAGTGATTTACCATTGGATAAGCTGAGTGAACAGAAACAAAAAAAGCACAAAGGG AAAGGAGTTGGACATGAATTTCAGAAAGTTTCAGTTGACAAGTCATTTTCTAGAGGATGGAGTCGTGATC AGCCTGGCCAAGCCCCAATGAGACAGAGGAGTGCAACAACCACTGGTTCTCCAGGAACCGAAAAGGCGAG GAGTATAGTACGGCAAAAAACTGTCGATATTGATGATGCTCAAATACTTCCCCGCTCAACTAGAGTCAGA CATTTTTCACAAAGTGAAGAAACTGGAAATGAAGTTTTTGGTGCTTTGAATGAGGAGCAGCCATTGCCTC GAAGTAGCAGCACTTCTGACATCTTGGAACCATTCACTGTTGAACGAGCCAAAGTCAATAAAGAGGACAT GAGCCAAAAACTGCCTCCTCTTAATAGTGATATTGGCGGCAGCAGTGCTAATGTTCCTGATCTGATGGAT GAGTTTATAGCAGAACGACTTCGAAGTGGTAATGCCTCGACTATGACAAGAAGAGGAAGTAGTCCAGGCA GCCTGGAAATTCCCAAAGACCTCCCTGATATTCTAAACAAGCAGAACCAGATGCGCCCTATTGATGACCC AGGTGTGCCCTCAGAATGGACTTCTCCTGCCAGTGCAGGGAGCAGTGATCTTATCAGCTCAGATAGTCAT TCGGATTCTTTCAGCGCTTTCCAATATGATGGCCGAAAATTTGACAATTTTGGCTTTGGAACCGACACTG GGGTTACGTCCTCTGCTGATGTGGATTCAGGTTCTGGCCATCATCAGAGTGCTGAAGAGCAGGAAGTGGC TAGTCTAACTACTCTTCATATAGATTCTGAAACAAGCAGTCTTAATCAGCAAGCTTTCTCTGCTGAAGTT GCAACTATTACTGGTTCAGAAAGTGCTTCCCCAGTCCACTCACCTCTGGGCTCCAGGTCACAGACTCCTT CCCCTTCTACATTGAATATAGATCACATGGAACAGAAGGATCTGCAGCTCGACGAGAAGCTCCACCACTC TGTTCTTCAGACACCAGATGATCTAGAAATTAGTGAATTTCCATCAGAATGTTGTAGTGTGATGGCAGGA GGTACTCTGACTGGATGGCATGCTGATGTTGCTACTGTAATGTGGCGAAGAATGCTAGGCATTTTGGGAG ATGTAAATTCAATCATGGATCCTGAAATACATGCTCAAGTTTTTGATTACCTCTGTGAACTTTGGCAGAA TCTAGCTAAGATTAGAGATAACCTTGGCATTTCAACTGATAACCTGACCTCCCCTTCTCCACCAGTTTTA ATTCCTCCACTGAGAATTCTTACACCTTGGCTTTTTAAGGCAACCATGTTGACTGATAAATATAAACAAG GTAAATTACATGCATATAAACTTATTTGTAATACAATGAAAAGAAGACAAGATGTTTCTCCAAATAGAGA TTTTCTAACACATTTCTACAATATAATGCATTGTGGATTACTTCATATTGACCAGGATATTGTCAATACA ATCATCAAACACTGCTCACCTCAATTTTTTTCACTTGGTTTGCCTGGTGCCACAATGCTTATTATGGATT TTATTGTAGCAGCTGGTAGAGTGGCTTCTTCAGCTTTTCTCAATGCACCAAGAGTAGAAGCACAAGTTCT TCTGGGATCTTTGGTTTGCTTTCCCAACTTATATTGTGAACTGCCTTCTCTTCATCCCAACATTCCTGAT GTTGCTGTGTCTCAGTTTACAGATGTTAAGGAACTTATAATCAAAACTGTATTAAGCTCGGCAAGAGATG AGCCCTCTGGTCCTGCACGATGTGTAGCACTTTGTAGTTTAGGTATTTGGATTTGTGAAGAACTAGTCCA TGAGTCTCATCATCCTCAAATTAAGGAAGCTCTGAATGTGATTTGTGTTTCCTTAAAGTTTACTAATAAA ACAGTAGCCCACGTAGCTTGTAACATGCTTCACATGCTGGTTCATTATGTACCTAGACTTCAGATTTACC AGCCTGATTCTCCCTTGAAAATTATTCAAATCCTAATAGCTACCATCACCCATCTTTTACCAAGTACAGA GGCTTCATCTTATGAAATGGACAAGAGGTTGGTAGTATCTTTACTTCTCTGCCTTCTGGACTGGATCATG GCCTTACCTCTAAAGACACTGCTCCAACCATTTCATGCTACGGGAGCAGAAAGCGATAAAACAGAAAAAT CTGTTCTCAATTGCATTTATAAGGTTTTACATGGGTGTGTTTATGGAGCTCAGTGTTTTAGCAATCCAAG GTATTTTCCCATGAGCCTCTCTGATTTGGCATCTGTAGATTATGATCCTTTTATGCATTTGGAAAGTCTG AAAGAGCCTGAGCCTCTGCACTCTCCTGACTCAGAACGATCTTCTAAACTCCAGCCAGTAACAGAAGTGA AAACTCAAATGCAGCATGGATTAATCTCTATAGCAGCCCGCACTGTTATTACACATCTGGTAAATCACCT GGGCCATTATCCAATGAGCGGTGGTCCTGCTATGCTAACAAGTCAGGTGTGTGAAAATCACGACAATCAT TACAGTGAAAGTACTGAACTTTCTCCTGAACTCTTTGAGAGTCCAAATATCCAGTTCTTTGTGTTAAATA ATACAACCTTAGTGTCCTGTATCCAGATCAGATCAGAAGAGAATATGCCTGGAGGAGGTTTATCTGCTGG CCTTGCATCAGCCAATTCAAATGTCAGAATCATAGTACGTGATCTCTCTGGAAAATATTCATGGGATTCT GCTATACTGTATGGCCCACCTCCTGTAAGTGGCTTGTCAGAACCTACATCTTTCATGCTTTCATTGTCTC ACCAAGAGAAGCCAGAAGAGCCTCCGACATCTAATGAATGCTTAGAAGATATAACCGTAAAAGATGGACT TTCTCTCCAGTTTAAAAGATTTAGAGAAACTGTACCAACTTGGGATACAATAAGAGATGAAGAAGATGTT CTTGATGAGCTCTTGCAGTATTTGGGTGTTACTAGTCCTGAATGCTTACAGAGAACTGGAATCTCACTTA ATATTCCTGCTCCACAACCTGTGTGCATTTCTGAAAAACAAGAAAATGATGTTATTAATGCTATCCTTAA GCAACATACAGAAGAAAAAGAATTTGTTGAGAAGCACTTTAATGACTTAAACATGAAAGCTGTGGAACAA GATGAACCAATACCTCAAAAACCTCAGTCAGCATTTTATTATTGCAGATTGCTTCTTAGTATATTGGGAA TGAATTCCTGGGACAAACGGAGGAGCTTTCATCTCCTGAAGAAAAATGAAAAGCTACTTAGAGAACTTAG GAACTTGGATTCAAGGCAGTGCCGAGAGACACACAAGATTGCAGTATTTTATGTTGCTGAAGGACAAGAA GACAAACACTCCATTCTCACCAATACAGGAGGAAGTCAAGCATATGAAGATTTTGTAGCTGGTCTTGGTT GGGAGGTAAATCTTACAAACCATTGTGGTTTTATGGGAGGACTACAAAAAAACAAAAGCACTGGATTGAC CACTCCATATTTTGCTACCTCTACAGTAGAGGTAATATTTCACGTGTCAACAAGAATGCCTTCTGATTCT GATGATTCTTTGACCAAAAAATTGAGACATTTGGGAAATGATGAAGTGCACATTGTTTGGTCAGAGCATA CTAGAGACTACAGGAGAGGAATTATTCCCACAGAATTTGGTGATGTCCTTATTGTAATATATCCAATGAA AAATCACATGTTCAGTATTCAGATAATGAAAAAACCAGAGGTTCCCTTCTTTGGTCCCCTTTTTGATGGT GCTATTGTGAATGGAAAGGTTCTACCCATTATGGTTAGAGCAACAGCTATAAATGCAAGCCGTGCTCTGA AATCTCTGATTCCATTGTATCAAAACTTCTATGAGGAGAGAGCACGATACCTGCAAACAATTGTCCAGCA CCACTTAGAACCAACAACATTTGAAGATTTTGCAGCACAGGTTTTTTCTCCAGCTCCCTACCACCATTTA CCATCTGATGCCGATCAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC216040 representing NM_014990 Red=Cloning site Green=Tags(s) MFSKKPHGDVKKSTQKVLDTKKDALTRLKHLRIVIENAESIDLKQFFDQHFSHIYYVFFENFVTIEASLK QKGHKSQREELDAILFIFEKILQLLPERIHQRWQFHSIGLILKKLLHTGNSLKIRREGVRLFLLWLQALQ NNCSKEQLWMFSCLIPGFSAPQSEHGPRTLDNLINPPLNLQETQVTIEEITPLVPPQSGDKGQEDLTSYF LEALLKYIVIQVKSLEWKNKENQERGFSFLFSHFKKYYLPYIFPNICKENSLYHPILDIPQMRPKPHYVV IKKDAETNEAIYCTKEPFIKARVIVIRWLVSFWLEPKPHTGPHIPGMEGEVLPKNIQRAAASLVSREESK NDNADKTDRTTEPEQSHSNTSTLTEREPSSSSLCSIDEEHLTDIEIVRRVFSSKRSNVNFVTEIFRQAFL LPICEAAAMRKVVKVYQEWIQQEEKPLFMQEPEEIVITSSDLPCIENVTDHDISMEEGEKREEENGTNTA DHVRNSSWAKNGSYQGALHNASEEATEQNIRAGTQAVLQVFIINSSNIFLLEPANEIKNLLDEHTDMCKR ILNIYRYMVVQVSMDKKTWEQMLLVLLRVTESVLKMPSQAFLQFQGKKNMTLAGRLAGPLFQTLIVAWIK ANLNVYISRELWDDLLSVLSSLTYWEELATEWSLTMETLTKVLARNLYSLDLSDLPLDKLSEQKQKKHKG KGVGHEFQKVSVDKSFSRGWSRDQPGQAPMRQRSATTTGSPGTEKARSIVRQKTVDIDDAQILPRSTRVR HFSQSEETGNEVFGALNEEQPLPRSSSTSDILEPFTVERAKVNKEDMSQKLPPLNSDIGGSSANVPDLMD EFIAERLRSGNASTMTRRGSSPGSLEIPKDLPDILNKQNQMRPIDDPGVPSEWTSPASAGSSDLISSDSH SDSFSAFQYDGRKFDNFGFGTDTGVTSSADVDSGSGHHQSAEEQEVASLTTLHIDSETSSLNQQAFSAEV ATITGSESASPVHSPLGSRSQTPSPSTLNIDHMEQKDLQLDEKLHHSVLQTPDDLEISEFPSECCSVMAG GTLTGWHADVATVMWRRMLGILGDVNSIMDPEIHAQVFDYLCELWQNLAKIRDNLGISTDNLTSPSPPVL IPPLRILTPWLFKATMLTDKYKQGKLHAYKLICNTMKRRQDVSPNRDFLTHFYNIMHCGLLHIDQDIVNT IIKHCSPQFFSLGLPGATMLIMDFIVAAGRVASSAFLNAPRVEAQVLLGSLVCFPNLYCELPSLHPNIPD VAVSQFTDVKELIIKTVLSSARDEPSGPARCVALCSLGIWICEELVHESHHPQIKEALNVICVSLKFTNK TVAHVACNMLHMLVHYVPRLQIYQPDSPLKIIQILIATITHLLPSTEASSYEMDKRLVVSLLLCLLDWIM ALPLKTLLQPFHATGAESDKTEKSVLNCIYKVLHGCVYGAQCFSNPRYFPMSLSDLASVDYDPFMHLESL KEPEPLHSPDSERSSKLQPVTEVKTQMQHGLISIAARTVITHLVNHLGHYPMSGGPAMLTSQVCENHDNH YSESTELSPELFESPNIQFFVLNNTTLVSCIQIRSEENMPGGGLSAGLASANSNVRIIVRDLSGKYSWDS AILYGPPPVSGLSEPTSFMLSLSHQEKPEEPPTSNECLEDITVKDGLSLQFKRFRETVPTWDTIRDEEDV LDELLQYLGVTSPECLQRTGISLNIPAPQPVCISEKQENDVINAILKQHTEEKEFVEKHFNDLNMKAVEQ DEPIPQKPQSAFYYCRLLLSILGMNSWDKRRSFHLLKKNEKLLRELRNLDSRQCRETHKIAVFYVAEGQE DKHSILTNTGGSQAYEDFVAGLGWEVNLTNHCGFMGGLQKNKSTGLTTPYFATSTVEVIFHVSTRMPSDS DDSLTKKLRHLGNDEVHIVWSEHTRDYRRGIIPTEFGDVLIVIYPMKNHMFSIQIMKKPEVPFFGPLFDG AIVNGKVLPIMVRATAINASRALKSLIPLYQNFYEERARYLQTIVQHHLEPTTFEDFAAQVFSPAPYHHL PSDADH TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_014990
ORF Size	6108 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_014990.3
RefSeq Size	7882 bp
RefSeq ORF	6111 bp
Locus ID	253959
UniProt ID	Q6GYQ0
MW	229.7 kDa
Gene Summary	This gene encodes a major subunit of the RAL-GTPase activating protein. A similar protein in mouse binds E12, a transcriptional regulator of immunoglobulin genes. The mouse protein also functions in skeletal muscle by binding to the regulatory 14-3-3 proteins upon stimulation with insulin or muscle contraction. A pseudogene of this gene has been identified on chromosome 9. [provided by RefSeq, Oct 2016]

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