ROBO3 (NM_022370) Human Tagged Lenti ORF Clone
CAT#: RC216411L4
- LentiORF®
Lenti-ORF clone of ROBO3 (mGFP-tagged)-Human roundabout, axon guidance receptor, homolog 3 (Drosophila) (ROBO3)
"NM_022370" in other vectors (6)
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CNY 15,770.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | mGFP |
Synonyms | HGPPS; HGPPS1; HGPS; RBIG1; RIG1 |
Vector | pLenti-C-mGFP-P2A-Puro |
E. coli Selection | Chloramphenicol (34 ug/mL) |
Mammalian Cell Selection | Puromycin |
Sequence Data |
The ORF insert of this clone is exactly the same as(RC216411).
|
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_022370 |
ORF Size | 4158 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_022370.3 |
RefSeq Size | 4554 bp |
RefSeq ORF | 4161 bp |
Locus ID | 64221 |
UniProt ID | Q96MS0 |
Protein Families | Druggable Genome |
Protein Pathways | Axon guidance |
MW | 148.2 kDa |
Gene Summary | This gene is a member of the Roundabout (ROBO) gene family that controls neurite outgrowth, growth cone guidance, and axon fasciculation. ROBO proteins are a subfamily of the immunoglobulin transmembrane receptor superfamily. SLIT proteins 1-3, a family of secreted chemorepellants, are ligands for ROBO proteins and SLIT/ROBO interactions regulate myogenesis, leukocyte migration, kidney morphogenesis, angiogenesis, and vasculogenesis in addition to neurogenesis. This gene, ROBO3, has a putative extracellular domain with five immunoglobulin (Ig)-like loops and three fibronectin (Fn) type III motifs, a transmembrane segment, and a cytoplasmic tail with three conserved signaling motifs: CC0, CC2, and CC3 (CC for conserved cytoplasmic). Unlike other ROBO family members, ROBO3 lacks motif CC1. The ROBO3 gene regulates axonal navigation at the ventral midline of the neural tube. In mouse, loss of Robo3 results in a complete failure of commissural axons to cross the midline throughout the spinal cord and the hindbrain. Mutations ROBO3 result in horizontal gaze palsy with progressive scoliosis (HGPPS); an autosomal recessive disorder characterized by congenital absence of horizontal gaze, progressive scoliosis, and failure of the corticospinal and somatosensory axon tracts to cross the midline in the medulla. [provided by RefSeq, May 2019] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC216411 | ROBO3 (Myc-DDK-tagged)-Human roundabout, axon guidance receptor, homolog 3 (Drosophila) (ROBO3) |
CNY 14,224.00 |
|
RC216411L1 | Lenti-ORF clone of ROBO3 (Myc-DDK-tagged)-Human roundabout, axon guidance receptor, homolog 3 (Drosophila) (ROBO3) |
CNY 16,624.00 |
|
RC216411L2 | Lenti-ORF clone of ROBO3 (mGFP-tagged)-Human roundabout, axon guidance receptor, homolog 3 (Drosophila) (ROBO3) |
CNY 15,770.00 |
|
RC216411L3 | Lenti-ORF clone of ROBO3 (Myc-DDK-tagged)-Human roundabout, axon guidance receptor, homolog 3 (Drosophila) (ROBO3) |
CNY 15,770.00 |
|
RG216411 | ROBO3 (tGFP-tagged) - Human roundabout, axon guidance receptor, homolog 3 (Drosophila) (ROBO3) |
CNY 15,300.00 |
|
SC308726 | ROBO3 (untagged)-Human roundabout, axon guidance receptor, homolog 3 (Drosophila) (ROBO3) |
CNY 15,576.00 |