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RAD1 (NM_002853) Human Tagged ORF Clone
CAT＃: RC216420
RAD1 (Myc-DDK-tagged)-Human RAD1 homolog (S. pombe) (RAD1), transcript variant 1
ORF Plasmid: tGFP
"NM_002853" in other vectors (6)
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CNY 2,400.00
CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | HRAD1; REC1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC216420 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCCCTTCTGACCCAACAGATCCAAGACGAGGATGATCAGTACAGCCTTGTGGCCAGCCTTGACAACG TTAGGAATCTCTCCACTATCTTGAAAGCTATTCATTTCCGAGAACATGCCACGTGTTTCGCAACTAAAAA TGGTATCAAAGTAACAGTGGAAAATGCAAAGTGTGTGCAAGCAAATGCTTTTATTCAGGCTGGAATATTT CAGGAGTTTAAAGTTCAGGAAGAGTCTGTTACTTTTCGAATTAATTTAACTGTCCTTTTAGACTGTTTAT CTATTTTTGGATCAAGTCCTATGCCAGGGACTTTAACTGCACTTCGAATGTGTTACCAAGGTTATGGTTA CCCTTTGATGCTGTTCCTGGAAGAAGGAGGAGTGGTGACAGTCTGCAAAATCAATACACAGGAACCTGAG GAGACCCTGGACTTTGATTTCTGCAGCACCAATGTTATTAATAAAATTATTCTGCAGTCAGAGGGGCTCC GTGAAGCATTTTCTGAATTGGATATGACGAGTGAAGTCCTACAAATTACCATGTCTCCTGACAAGCCTTA TTTCAGGTTATCTACTTTTGGAAATGCAGGAAGTTCCCACCTTGACTATCCCAAAGATTCTGATTTGATG GAAGCATTTCATTGTAATCAGACCCAAGTCAACAGATACAAGATTTCCTTACTGAAACCCTCTACAAAGG CATTAGTCCTATCTTGTAAGGTATCTATTCGGACAGATAACAGAGGCTTCCTTTCATTACAGTATATGAT TAGAAATGAAGATGGACAAATATGTTTTGTGGAATATTACTGCTGCCCTGATGAAGAAGTTCCTGAATCT GAGTCT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC216420 protein sequence
Red=Cloning site Green=Tags(s) MPLLTQQIQDEDDQYSLVASLDNVRNLSTILKAIHFREHATCFATKNGIKVTVENAKCVQANAFIQAGIF QEFKVQEESVTFRINLTVLLDCLSIFGSSPMPGTLTALRMCYQGYGYPLMLFLEEGGVVTVCKINTQEPE ETLDFDFCSTNVINKIILQSEGLREAFSELDMTSEVLQITMSPDKPYFRLSTFGNAGSSHLDYPKDSDLM EAFHCNQTQVNRYKISLLKPSTKALVLSCKVSIRTDNRGFLSLQYMIRNEDGQICFVEYYCCPDEEVPES ES TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_002853 |
| ORF Size | 846 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_002853.4 |
| RefSeq Size | 4683 bp |
| RefSeq ORF | 849 bp |
| Locus ID | 5810 |
| UniProt ID | O60671 |
| Domains | Rad1 |
| Protein Families | Druggable Genome |
| MW | 31.8 kDa |
| Gene Summary | This gene encodes a component of a heterotrimeric cell cycle checkpoint complex, known as the 9-1-1 complex, that is activated to stop cell cycle progression in response to DNA damage or incomplete DNA replication. The 9-1-1 complex is recruited by RAD17 to affected sites where it may attract specialized DNA polymerases and other DNA repair effectors. Alternatively spliced transcript variants of this gene have been described. [provided by RefSeq, Jan 2009] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC216420L1 | Lenti ORF clone of Human RAD1 homolog (S. pombe) (RAD1), transcript variant 1, Myc-DDK-tagged |
CNY 4,800.00 |
|
| RC216420L2 | Lenti ORF clone of Human RAD1 homolog (S. pombe) (RAD1), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RC216420L3 | Lenti ORF clone of Human RAD1 homolog (S. pombe) (RAD1), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC216420L4 | Lenti ORF clone of Human RAD1 homolog (S. pombe) (RAD1), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RG216420 | RAD1 (tGFP-tagged) - Human RAD1 homolog (S. pombe) (RAD1), transcript variant 1 |
CNY 4,000.00 |
|
| SC118373 | RAD1 (untagged)-Human RAD1 homolog (S. pombe) (RAD1), transcript variant 1 |
CNY 2,400.00 |
