Endothelin B Receptor (EDNRB) (NM_003991) Human Tagged ORF Clone
CAT#: RC216462
EDNRB (Myc-DDK-tagged)-Human endothelin receptor type B (EDNRB), transcript variant 2
ORF Plasmid: tGFP
"NM_003991" in other vectors (4)
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CNY 3,656.00
CNY 3,990.00
Cited in 1 publication. |
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | ABCDS; ET-B; ET-BR; ETB; ETB1; ETBR; ETRB; HSCR; HSCR2; WS4A |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC216462 representing NM_003991
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAGCCGCCTCCAAGTCTGTGCGGACGCGCCCTGGTTGCGCTGGTTCTTGCCTGCGGCCTGTCGCGGA TCTGGGGAGAGGAGAGAGGCTTCCCGCCTGACAGGGCCACTCCGCTTTTGCAAACCGCAGAGATAATGAC GCCACCCACTAAGACCTTATGGCCCAAGGGTTCCAACGCCAGTCTGGCGCGGTCGTTGGCACCTGCGGAG GTGCCTAAAGGAGACAGGACGGCAGGATCTCCGCCACGCACCATCTCCCCTCCCCCGTGCCAAGGACCCA TCGAGATCAAGGAGACTTTCAAATACATCAACACGGTTGTGTCCTGCCTTGTGTTCGTGCTGGGGATCAT CGGGAACTCCACACTTCTGAGAATTATCTACAAGAACAAGTGCATGCGAAACGGTCCCAATATCTTGATC GCCAGCTTGGCTCTGGGAGACCTGCTGCACATCGTCATTGACATCCCTATCAATGTCTACAAGCTGCTGG CAGAGGACTGGCCATTTGGAGCTGAGATGTGTAAGCTGGTGCCTTTCATACAGAAAGCCTCCGTGGGAAT CACTGTGCTGAGTCTATGTGCTCTGAGTATTGACAGATATCGAGCTGTTGCTTCTTGGAGTAGAATTAAA GGAATTGGGGTTCCAAAATGGACAGCAGTAGAAATTGTTTTGATTTGGGTGGTCTCTGTGGTTCTGGCTG TCCCTGAAGCCATAGGTTTTGATATAATTACGATGGACTACAAAGGAAGTTATCTGCGAATCTGCTTGCT TCATCCCGTTCAGAAGACAGCTTTCATGCAGTTTTACAAGACAGCAAAAGATTGGTGGCTATTCAGTTTC TATTTCTGCTTGCCATTGGCCATCACTGCATTTTTTTATACACTAATGACCTGTGAAATGTTGAGAAAGA AAAGTGGCATGCAGATTGCTTTAAATGATCACCTAAAGCAGAGACGGGAAGTGGCCAAAACCGTCTTTTG CCTGGTCCTTGTCTTTGCCCTCTGCTGGCTTCCCCTTCACCTCAGCAGGATTCTGAAGCTCACTCTTTAT AATCAGAATGATCCCAATAGATGTGAACTTTTGAGCTTTCTGTTGGTATTGGACTATATTGGTATCAACA TGGCTTCACTGAATTCCTGCATTAACCCAATTGCTCTGTATTTGGTGAGCAAAAGATTCAAAAACTGCTT TAAGGCTGGGCCACATGTTGGAAATAAGCTAGTAATGTTGTTTTCTGTCAATATTGAATGTGATGGTACA GTAAACCAAAACCCAACAATGTGGCCAGAAAGAAAGAGCAATAATAAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC216462 representing NM_003991
Red=Cloning site Green=Tags(s) MQPPPSLCGRALVALVLACGLSRIWGEERGFPPDRATPLLQTAEIMTPPTKTLWPKGSNASLARSLAPAE VPKGDRTAGSPPRTISPPPCQGPIEIKETFKYINTVVSCLVFVLGIIGNSTLLRIIYKNKCMRNGPNILI ASLALGDLLHIVIDIPINVYKLLAEDWPFGAEMCKLVPFIQKASVGITVLSLCALSIDRYRAVASWSRIK GIGVPKWTAVEIVLIWVVSVVLAVPEAIGFDIITMDYKGSYLRICLLHPVQKTAFMQFYKTAKDWWLFSF YFCLPLAITAFFYTLMTCEMLRKKSGMQIALNDHLKQRREVAKTVFCLVLVFALCWLPLHLSRILKLTLY NQNDPNRCELLSFLLVLDYIGINMASLNSCINPIALYLVSKRFKNCFKAGPHVGNKLVMLFSVNIECDGT VNQNPTMWPERKSNNN TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_003991 |
ORF Size | 1308 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_003991.3, NP_003982.1 |
RefSeq Size | 1578 bp |
RefSeq ORF | 1311 bp |
Locus ID | 1910 |
UniProt ID | P24530 |
Domains | 7tm_1 |
Protein Families | Druggable Genome, GPCR, Transmembrane |
Protein Pathways | Calcium signaling pathway, Melanogenesis, Neuroactive ligand-receptor interaction |
MW | 48.71 kDa |
Gene Summary | The protein encoded by this gene is a G protein-coupled receptor which activates a phosphatidylinositol-calcium second messenger system. Its ligand, endothelin, consists of a family of three potent vasoactive peptides: ET1, ET2, and ET3. Studies suggest that the multigenic disorder, Hirschsprung disease type 2, is due to mutations in the endothelin receptor type B gene. Alternative splicing and the use of alternative promoters results in multiple transcript variants. [provided by RefSeq, Oct 2016] |
Citations (1)
The use of this cDNA Clones has been cited in the following citations: |
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Differences in Endothelin B Receptor Isoforms Expression and Function in Breast Cancer Cells
,Halaka, M;Hired, ZA;Rutledge, GE;Hedgepath, CM;Anderson, MP;St John, H;Do, JM;Majmudar, PR;Walker, C;Alawawdeh, A;Stephen, HM;Reagor, CC;Adereti, J;Jamison, K;Iglesias, KP;Kirmani, KZ;Conway, RE;,
J Cancer
,PubMed ID 32201539
[Endothelin B Receptor]
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Documents
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Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC216462L3 | Lenti ORF clone of Human endothelin receptor type B (EDNRB), transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
|
RC216462L4 | Lenti ORF clone of Human endothelin receptor type B (EDNRB), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
RG216462 | EDNRB (tGFP-tagged) - Human endothelin receptor type B (EDNRB), transcript variant 2 |
CNY 4,370.00 |
|
SC310392 | EDNRB (untagged)-Human endothelin receptor type B (EDNRB), transcript variant 2 |
CNY 7,030.00 |
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