ASCC3 (NM_006828) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	ASC1p200; HELIC1; RNAH
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC216672 representing NM_006828 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTTTACCTCGTCTCACAGGAGCCTTGCGTTCCTTTTCAAATGTCACCAAGCAAGATAATTATAATG AAGAAGTGGCTGACTTAAAGATAAAGCGATCTAAACTTCATGAACAAGTTTTAGATTTGGGCCTGACATG GAAGAAGATAATAAAATTTTTGAATGAAAAACTGGAGAAGAGTAAAATGCAAAGTATAAATGAAGACTTA AAAGATATATTACATGCTGCAAAGCAGATAGTTGGAACTGATAATGGGAGAGAAGCAATTGAAAGTGGGG CTGCATTTCTCTTCATGACATTTCACTTGAAGGACTCTGTTGGTCACAAGGAAACAAAGGCTATCAAACA GATGTTTGGCCCCTTTCCTTCATCATCTGCCACTGCAGCTTGTAATGCTACTAATCGAATTATTTCTCAT TTTAGTCAAGATGATCTTACTGCTCTTGTGCAGATGACAGAAAAAGAACATGGCGATAGGGTTTTTTTTG GTAAAAATTTAGCATTTTCATTTGACATGCATGATTTGGACCACTTTGACGAACTGCCAATAAATGGTGA AACTCAGAAAACTATAAGCCTAGATTATAAGAAGTTTCTGAATGAACATCTCCAGGAGGCTTGCACCCCA GAACTCAAGCCTGTGGAAAAAACAAATGGCTCCTTTTTGTGGTGTGAAGTTGAAAAGTACCTAAATTCAA CTTTGAAGGAAATGACTGAAGTGCCAAGAGTAGAAGATCTTTGCTGTACTTTATATGATATGCTTGCTTC TATTAAAAGTGGTGATGAACTTCAGGATGAGCTATTTGAACTGCTGGGACCTGAAGGACTTGAACTTATT GAGAAACTCCTCCAGAACAGAATTACAATTGTGGATAGATTTCTTAATTCTTCAAATGATCATAGGTTTC AGGCTCTTCAAGACAATTGTAAAAAAATTTTAGGAGAAAATGCTAAACCCAATTATGGTTGTCAAGTCAC TATTCAGTCTGAACAAGAAAAGCAGTTAATGAAACAATATCGACGTGAAGAAAAAAGAATTGCCAGACGA GAAAAAAAGGCTGGAGAAGATTTAGAAGTTTCAGAAGGACTTATGTGCTTTGATCCTAAGGAATTGCGGA TACAAAGAGAACAGGCACTTCTGAATGCTAGAAGTGTTCCAATTCTGAGCAGGCAGAGAGATGCAGACGT TGAAAAAATACATTATCCCCATGTGTATGATTCCCAGGCTGAAGCCATGAAAACATCAGCATTTATTGCT GGTGCAAAGATGATTTTGCCAGAAGGAATCCAAAGAGAGAATAACAAGCTTTATGAAGAAGTAAGGATTC CCTACAGCGAACCAATGCCACTCAGCTTTGAGGAAAAGCCAGTTTATATCCAAGACTTAGATGAGATCGG ACAGCTGGCTTTTAAAGGAATGAAGAGACTCAATAGAATCCAGTCAATAGTGTTTGAGACTGCCTACAAC ACCAATGAGAACATGCTGATTTGTGCCCCTACAGGAGCTGGAAAAACCAACATTGCAATGCTGACAGTCT TGCATGAAATTCGCCAACATTTTCAACAAGGTGTTATCAAAAAGAATGAATTTAAGATTGTATATGTTGC TCCAATGAAAGCCTTGGCAGCTGAAATGACAGATTACTTCAGCAGACGTCTAGAGCCACTAGGCATCATT GTGAAAGAATTGACTGGTGACATGCAGTTGTCCAAAAGTGAAATTTTACGAACTCAGATGCTTGTGACCA CACCAGAAAAATGGGATGTAGTGACAAGAAAGAGTGTTGGGGATGTAGCTCTTTCCCAGATTGTAAGGCT CCTTATTCTTGATGAAGTTCATTTGCTGCATGAAGATAGAGGACCAGTATTAGAAAGCATAGTTGCCCGT ACTTTACGGCAGGTGGAATCCACACAGAGTATGATAAGGATTCTCGGACTGTCTGCAACTTTACCTAACT ACCTCGATGTTGCCACATTTTTACATGTTAATCCATACATTGGACTTTTCTTCTTTGATGGCCGTTTTCG ACCAGTACCTCTTGGACAGACATTTTTGGGGATTAAATGTGCAAATAAGATGCAGCAGTTGAATAACATG GATGAAGTATGTTATGAAAATGTTTTGAAGCAAGTAAAGGCTGGACACCAGGTGATGGTGTTTGTACATG CTCGAAATGCCACTGTAAGAACAGCTATGTCTCTAATAGAAAGAGCAAAAAATTGTGGCCATATTCCCTT CTTTTTTCCTACCCAAGGACATGACTATGTACTTGCAGAAAAACAGGTACAAAGGTCGAGAAATAAGCAA GTACGAGAATTATTCCCAGATGGTTTTAGTATTCATCATGCAGGAATGCTTCGGCAGGACAGAAATTTAG TTGAAAACTTGTTTTCTAATGGGCATATCAAAGTCCTAGTGTGTACAGCTACGTTAGCCTGGGGTGTCAA TCTTCCCGCCCATGCTGTTATTATTAAGGGAACACAAATATATGCTGCAAAAAGAGGCTCCTTTGTTGAC CTTGGAATTTTAGATGTCATGCAGATATTTGGTCGAGCTGGACGACCACAATTTGACAAATTTGGGGAAG GAATAATTATAACAACGCATGATAAACTCAGCCATTACCTCACTTTGCTCACTCAACGAAACCCAATTGA GAGTCAGTTTCTGGAAAGCCTTGCAGATAACCTAAATGCAGAGATTGCTCTGGGAACAGTTACTAATGTG GAAGAAGCAGTGAAGTGGATAAGTTACACTTATCTTTATGTACGGATGAGAGCAAATCCATTAGCATATG GCATCAGTCACAAGGCTTATCAGATTGACCCAACATTAAGAAAGCATCGAGAACAGTTGGTCATTGAAGT TGGACGAAAACTAGACAAAGCTCAGATGATTCGTTTTGAGGAGCGAACTGGATATTTTTCCTCAACTGAT TTGGGTAGAACTGCCAGCCATTACTATATTAAATACAACACCATTGAGACCTTTAATGAACTCTTTGATG CTCACAAAACAGAAGGTGATATCTTTGCCATAGTCTCCAAAGCTGAAGAATTTGATCAAATTAAGGTCAG AGAAGAGGAAATAGAGGAGTTAGATACCTTATTAAGCAATTTTTGTGAACTCTCCACTCCTGGAGGTGTA GAGAATAGTTATGGGAAAATAAACATCTTACTTCAAACTTATATCAGCCGAGGAGAAATGGACAGTTTCT CCCTTATATCAGATTCTGCATATGTTGCACAGAATGCAGCTAGAATTGTCCGTGCTCTTTTTGAAATTGC TCTGAGGAAACGTTGGCCTACCATGACCTACAGGCTCCTGAATCTTAGTAAAGTCATTGACAAGAGGCTT TGGGGTTGGGCTAGCCCTTTGAGACAATTTTCAATCCTACCACCACACATCCTAACAAGATTAGAAGAAA AAAAGCTTACTGTGGATAAGCTGAAAGACATGAGGAAAGATGAAATAGGTCACATTTTACATCATGTGAA TATTGGACTGAAGGTCAAACAATGTGTTCATCAGATTCCTTCTGTTATGATGGAAGCATCCATTCAGCCT ATCACAAGGACTGTCCTCCGAGTGACACTCAGCATCTATGCTGATTTCACTTGGAATGATCAGGTACATG GGACAGTAGGAGAACCTTGGTGGATTTGGGTAGAAGATCCTACAAATGATCATATTTATCATTCAGAGTA TTTTCTAGCTCTAAAAAAACAAGTCATTAGTAAAGAAGCCCAACTACTGGTATTTACAATCCCTATTTTT GAGCCTTTGCCTTCCCAATACTACATCCGAGCAGTGTCTGATAGATGGTTGGGTGCTGAGGCAGTATGTA TTATCAACTTTCAACATCTAATTCTACCAGAGAGACATCCTCCTCATACAGAATTACTGGATCTTCAGCC TTTACCAATCACAGCTTTGGGATGTAAAGCATATGAAGCCCTGTACAACTTCAGCCACTTTAACCCTGTA CAGACACAAATATTTCATACATTGTATCACACGGATTGTAATGTCCTACTTGGAGCACCTACTGGATCGG GAAAGACTGTTGCAGCTGAATTAGCCATTTTCAGAGTCTTCAACAAATACCCTACTTCAAAGGCGGTATA TATTGCACCCCTAAAAGCCCTAGTACGTGAAAGAATGGATGATTGGAAAGTTAGAATAGAAGAAAAACTT GGTAAAAAAGTTATTGAACTAACAGGGGATGTGACTCCTGATATGAAATCCATTGCCAAGGCTGACCTTA TCGTCACTACGCCAGAGAAGTGGGATGGAGTCAGCAGAAGCTGGCAAAATAGGAACTATGTTCAGCAAGT CACTATTCTCATCATAGATGAGATCCATCTGCTTGGGGAGGAAAGAGGCCCTGTTCTAGAGGTCATTGTA TCTCGAACAAATTTTATCTCATCACACACAGAAAAGCCTGTTAGAATAGTTGGACTATCTACTGCATTAG CTAATGCCAGAGACCTTGCTGATTGGCTCAATATTAAGCAGATGGGCTTGTTTAACTTCCGACCATCAGT ACGCCCAGTTCCACTGGAAGTTCACATTCAAGGCTTTCCAGGTCAACATTACTGTCCTCGTATGGCTAGT ATGAACAAGCCTGCATTTCAGGCAATTAGAAGCCATTCTCCAGCCAAACCTGTTTTGATATTTGTCTCAT CAAGACGTCAAACTCGTCTTACTGCTTTGGAATTGATCGCCTTCCTGGCTACTGAAGAAGATCCAAAGCA GTGGTTAAACATGGATGAAAGAGAGATGGAGAACATCATTGCAACAGTAAGAGATTCCAACCTCAAGCTG ACCCTTGCTTTCGGGATAGGAATGCATCATGCTGGACTACATGAGAGGGACCGAAAAACAGTAGAGGAAC TATTTGTAAACTGTAAAGTTCAGGTTCTTATTGCTACAAGCACATTAGCCTGGGGTGTAAACTTTCCAGC TCATTTAGTAATTATTAAGGGAACAGAATACTATGATGGAAAAACAAGACGTTATGTGGATTTTCCCATT ACAGATGTCCTCCAGATGATGGGGCGTGCTGGGAGGCCGCAGTTCGATGACCAAGGCAAAGCTGTAATTC TAGTTCATGACATAAAGAAAGACTTTTATAAAAAATTTCTTTATGAACCTTTCCCAGTAGAATCAAGTTT ATTAGGAGTGCTCTCTGACCACTTAAATGCAGAGATTGCTGGTGGTACAATTACATCTAAGCAAGATGCA TTGGATTATATCACCTGGACTTACTTTTTCCGACGTCTTATCATGAATCCCAGCTATTACAATTTGGGTG ATGTGAGCCATGATTCTGTGAACAAGTTTCTGTCCCATCTGATTGAGAAGTCCCTGATTGAATTGGAACT TTCCTACTGTATTGAAATTGGAGAGGATAATCGCAGCATTGAACCTCTAACTTATGGCCGAATTGCCTCC TATTACTATTTGAAGCATCAAACAGTTAAAATGTTCAAGGACCGCTTGAAGCCTGAATGCAGTACTGAAG AACTGCTTTCAATTCTAAGTGATGCAGAAGAATATACAGATTTGCCAGTGAGACACAATGAAGATCATAT GAATAGTGAACTGGCAAAATGTCTTCCCATTGAATCAAATCCTCATTCATTTGACAGCCCTCACACCAAA GCACATCTCCTGCTACAGGCACATCTCAGCCGAGCCATGCTACCCTGCCCAGATTATGACACTGATACCA AAACAGTCTTGGACCAAGCTCTCAGAGTATGTCAGGCAATGCTGGACGTGGCTGCAAACCAGGGCTGGCT GGTGACTGTCCTGAATATCACCAACCTGATTCAGATGGTGATCCAGGGTCGGTGGTTAAAGGACTCTTCT CTTCTTACACTACCAAACATAGAAAACCATCATCTTCACCTTTTCAAGAAATGGAAGCCGATTATGAAGG GCCCACATGCTAGGGGTCGGACCTCCATCGAGTCCCTTCCTGAACTGATCCATGCCTGTGGAGGGAAAGA CCATGTATTTAGCTCCATGGTAGAAAGTGAGCTACATGCTGCAAAAACGAAACAGGCATGGAATTTCTTA TCTCACTTGCCAGTGATAAATGTTGGCATAAGTGTTAAAGGCTCGTGGGATGACTTAGTTGAAGGACATA ATGAACTCTCTGTCTCAACTCTGACTGCAGACAAACGAGATGACAACAAATGGATCAAATTGCATGCTGA CCAAGAGTATGTGCTTCAAGTGAGCTTGCAGAGAGTCCACTTTGGGTTCCACAAGGGAAAGCCAGAGAGC TGTGCAGTTACTCCTCGATTTCCCAAATCAAAAGACGAAGGATGGTTTTTGATATTAGGAGAAGTGGATA AGAGAGAACTTATTGCTTTGAAAAGAGTAGGATATATTCGAAATCATCATGTTGCTTCCCTTTCTTTTTA TACCCCTGAAATACCTGGAAGGTATATCTACACATTATATTTCATGAGTGACTGCTACCTTGGCCTGGAC CAGCAGTATGACATCTATCTCAACGTTACACAAGCGAGTCTTTCTGCACAGGTCAACACCAAGGTCTCTG ATTCCCTGACTGACCTGGCATTAAAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC216672 representing NM_006828 Red=Cloning site Green=Tags(s) MALPRLTGALRSFSNVTKQDNYNEEVADLKIKRSKLHEQVLDLGLTWKKIIKFLNEKLEKSKMQSINEDL KDILHAAKQIVGTDNGREAIESGAAFLFMTFHLKDSVGHKETKAIKQMFGPFPSSSATAACNATNRIISH FSQDDLTALVQMTEKEHGDRVFFGKNLAFSFDMHDLDHFDELPINGETQKTISLDYKKFLNEHLQEACTP ELKPVEKTNGSFLWCEVEKYLNSTLKEMTEVPRVEDLCCTLYDMLASIKSGDELQDELFELLGPEGLELI EKLLQNRITIVDRFLNSSNDHRFQALQDNCKKILGENAKPNYGCQVTIQSEQEKQLMKQYRREEKRIARR EKKAGEDLEVSEGLMCFDPKELRIQREQALLNARSVPILSRQRDADVEKIHYPHVYDSQAEAMKTSAFIA GAKMILPEGIQRENNKLYEEVRIPYSEPMPLSFEEKPVYIQDLDEIGQLAFKGMKRLNRIQSIVFETAYN TNENMLICAPTGAGKTNIAMLTVLHEIRQHFQQGVIKKNEFKIVYVAPMKALAAEMTDYFSRRLEPLGII VKELTGDMQLSKSEILRTQMLVTTPEKWDVVTRKSVGDVALSQIVRLLILDEVHLLHEDRGPVLESIVAR TLRQVESTQSMIRILGLSATLPNYLDVATFLHVNPYIGLFFFDGRFRPVPLGQTFLGIKCANKMQQLNNM DEVCYENVLKQVKAGHQVMVFVHARNATVRTAMSLIERAKNCGHIPFFFPTQGHDYVLAEKQVQRSRNKQ VRELFPDGFSIHHAGMLRQDRNLVENLFSNGHIKVLVCTATLAWGVNLPAHAVIIKGTQIYAAKRGSFVD LGILDVMQIFGRAGRPQFDKFGEGIIITTHDKLSHYLTLLTQRNPIESQFLESLADNLNAEIALGTVTNV EEAVKWISYTYLYVRMRANPLAYGISHKAYQIDPTLRKHREQLVIEVGRKLDKAQMIRFEERTGYFSSTD LGRTASHYYIKYNTIETFNELFDAHKTEGDIFAIVSKAEEFDQIKVREEEIEELDTLLSNFCELSTPGGV ENSYGKINILLQTYISRGEMDSFSLISDSAYVAQNAARIVRALFEIALRKRWPTMTYRLLNLSKVIDKRL WGWASPLRQFSILPPHILTRLEEKKLTVDKLKDMRKDEIGHILHHVNIGLKVKQCVHQIPSVMMEASIQP ITRTVLRVTLSIYADFTWNDQVHGTVGEPWWIWVEDPTNDHIYHSEYFLALKKQVISKEAQLLVFTIPIF EPLPSQYYIRAVSDRWLGAEAVCIINFQHLILPERHPPHTELLDLQPLPITALGCKAYEALYNFSHFNPV QTQIFHTLYHTDCNVLLGAPTGSGKTVAAELAIFRVFNKYPTSKAVYIAPLKALVRERMDDWKVRIEEKL GKKVIELTGDVTPDMKSIAKADLIVTTPEKWDGVSRSWQNRNYVQQVTILIIDEIHLLGEERGPVLEVIV SRTNFISSHTEKPVRIVGLSTALANARDLADWLNIKQMGLFNFRPSVRPVPLEVHIQGFPGQHYCPRMAS MNKPAFQAIRSHSPAKPVLIFVSSRRQTRLTALELIAFLATEEDPKQWLNMDEREMENIIATVRDSNLKL TLAFGIGMHHAGLHERDRKTVEELFVNCKVQVLIATSTLAWGVNFPAHLVIIKGTEYYDGKTRRYVDFPI TDVLQMMGRAGRPQFDDQGKAVILVHDIKKDFYKKFLYEPFPVESSLLGVLSDHLNAEIAGGTITSKQDA LDYITWTYFFRRLIMNPSYYNLGDVSHDSVNKFLSHLIEKSLIELELSYCIEIGEDNRSIEPLTYGRIAS YYYLKHQTVKMFKDRLKPECSTEELLSILSDAEEYTDLPVRHNEDHMNSELAKCLPIESNPHSFDSPHTK AHLLLQAHLSRAMLPCPDYDTDTKTVLDQALRVCQAMLDVAANQGWLVTVLNITNLIQMVIQGRWLKDSS LLTLPNIENHHLHLFKKWKPIMKGPHARGRTSIESLPELIHACGGKDHVFSSMVESELHAAKTKQAWNFL SHLPVINVGISVKGSWDDLVEGHNELSVSTLTADKRDDNKWIKLHADQEYVLQVSLQRVHFGFHKGKPES CAVTPRFPKSKDEGWFLILGEVDKRELIALKRVGYIRNHHVASLSFYTPEIPGRYIYTLYFMSDCYLGLD QQYDIYLNVTQASLSAQVNTKVSDSLTDLALK TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms	CHROMATOGRAMS Sequencher program is needed, download here.
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_006828
ORF Size	6606 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_006828.4
RefSeq Size	7598 bp
RefSeq ORF	6609 bp
Locus ID	10973
UniProt ID	Q8N3C0
Domains	DEAD, helicase_C, AAA, Sec63
Protein Families	Transcription Factors
MW	251.3 kDa
Gene Summary	This gene encodes a protein that belongs to a family of helicases that are involved in the ATP-dependent unwinding of nucleic acid duplexes. The encoded protein is the largest subunit of the activating signal cointegrator 1 complex that is involved in DNA repair and resistance to alkylation damage. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013]

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