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HUNK (NM_014586) Human Tagged ORF Clone
CAT＃: RC216803
HUNK (Myc-DDK-tagged)-Human hormonally up-regulated Neu-associated kinase (HUNK)
ORF Plasmid: tGFP
"NM_014586" in other vectors (6)
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CNY 5,232.00
CNY 5,800.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC216803 representing NM_014586
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCGGCGGCGGCGGGGGACGGGCTCCTGGGGGAGCCGGCGGCGCCTGGGGGCGGCGGCGGCGCGGAGG ACGCGGCCAGGCCCGCGGCGGCCTGCGAGGGAAGTTTCCTGCCTGCCTGGGTGAGCGGCGTGCCCCGCGA GCGGCTCCGCGACTTCCAGCACCACAAGCGCGTGGGCAACTACCTCATCGGCAGCAGGAAGCTGGGCGAG GGCTCCTTTGCCAAGGTGCGCGAGGGGCTGCACGTGCTGACCGGGGAGAAGGTGGCCATAAAAGTCATTG ATAAGAAGAGAGCCAAAAAGGACACCTATGTCACCAAAAACCTGCGGCGAGAGGGTCAGATCCAGCAGAT GATCCGCCACCCCAATATCACTCAGCTCCTTGATATTTTAGAAACGGAAAACAGCTACTACCTGGTCATG GAGCTGTGCCCTGGGGGCAACCTGATGCACAAGATCTATGAGAAGAAGCGGCTGGAGGAGTCCGAAGCCC GCAGATACATCCGACAGCTCATCTCTGCCGTAGAGCACCTGCACCGGGCCGGGGTGGTCCACAGAGACTT GAAGATAGAGAATTTGCTACTAGATGAAGACAATAATATCAAGCTGATTGACTTTGGTTTGAGCAACTGC GCAGGGATCCTGGGTTACTCGGATCCGTTCAGCACACAGTGTGGCAGCCCTGCCTACGCTGCACCTGAAC TGCTCGCCAGGAAGAAATACGGCCCCAAAATCGATGTCTGGTCCATAGGTGTGAACATGTATGCCATGTT GACCGGGACGCTGCCTTTCACGGTGGAGCCTTTCAGCCTGAGGGCTTTGTACCAGAAGATGGTAGACAAA GAAATGAACCCCCTCCCCACTCAGCTCTCCACAGGTGCCATCAGTTTCCTGCGCTCTCTCCTGGAACCGG ATCCTGTGAAGAGGCCAAATATTCAGCAGGCACTGGCGAATCGCTGGCTTAATGAGAATTACACGGGCAA AGTGCCCTGTAATGTCACCTATCCCAACAGGATTTCTCTGGAAGATCTGAGCCCGAGCGTCGTGCTGCAC ATGACCGAGAAGCTGGGTTACAAGAACAGCGACGTGATCAACACTGTGCTCTCCAACCGCGCCTGCCACA TCCTGGCCATCTACTTCCTCTTAAACAAGAAACTGGAGCGCTATTTGTCAGGGAAATCTGACATCCAGGA CAGCCTCTGCTACAAGACCCGGCTCTACCAGATAGAAAAGTACAGGGCCCCCAAGGAGTCCTATGAGGCC TCTCTGGACACCTGGACACGAGATCTTGAATTCCATGCCGTGCAGGATAAAAAGCCCAAAGAACAAGAAA AAAGAGGGGATTTTCTTCATCGACCATTCTCCAAGAAGTTGGACAAGAACCTGCCCTCGCACAAACAGCC CTCAGGCTCGCTCATGACACAGATTCAGAACACCAAAGCCCTCCTGAAGGACCGGAAGGCCTCCAAGTCC AGCTTCCCCGACAAAGATTCCTTTGGCTGCCGCAATATTTTCCGCAAAACCTCAGATTCCAATTGTGTGG CTTCTTCTTCCATGGAGTTCATCCCCGTGCCACCGCCCAGGACCCCGAGGATTGTGAAGAAACCGGAGCC CCATCAGCCAGGGCCCGGAAGCACTGGCATCCCCCACAAGGAAGACCCCCTGATGCTGGACATGGTGCGC TCCTTCGAGTCTGTGGATCGCGACGACCACGTAGAAGTGCTGTCTCCCTCTCATCACTACAGGATTCTGA ACTCCCCGGTCAGCTTGGCTCGCAGAAATTCCAGCGAGAGGACGCTGTCCCCGGGTCTGCCATCCGGAAG CATGTCGCCTCTCCATACTCCTTTGCATCCAACTCTGGTCTCTTTTGCTCACGAAGATAAGAACAGCCCC CCAAAAGAGGAGGGCCTGTGTTGCCCACCTCCGGTTCCCAGCAATGGCCCCATGCAGCCTCTGGGGAGCC CCAATTGTGTGAAAAGCCGAGGCCGGTTCCCTATGATGGGCATCGGACAGATGTTAAGGAAGCGCCATCA GAGTCTGCAGCCATCTGCAGATAGGCCCCTGGAGGCCAGCCTGCCCCCACTGCAGCCCCTAGCCCCTGTG AACCTTGCCTTTGACATGGCCGATGGGGTCAAGACCCAGTGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC216803 representing NM_014586
Red=Cloning site Green=Tags(s) MPAAAGDGLLGEPAAPGGGGGAEDAARPAAACEGSFLPAWVSGVPRERLRDFQHHKRVGNYLIGSRKLGE GSFAKVREGLHVLTGEKVAIKVIDKKRAKKDTYVTKNLRREGQIQQMIRHPNITQLLDILETENSYYLVM ELCPGGNLMHKIYEKKRLEESEARRYIRQLISAVEHLHRAGVVHRDLKIENLLLDEDNNIKLIDFGLSNC AGILGYSDPFSTQCGSPAYAAPELLARKKYGPKIDVWSIGVNMYAMLTGTLPFTVEPFSLRALYQKMVDK EMNPLPTQLSTGAISFLRSLLEPDPVKRPNIQQALANRWLNENYTGKVPCNVTYPNRISLEDLSPSVVLH MTEKLGYKNSDVINTVLSNRACHILAIYFLLNKKLERYLSGKSDIQDSLCYKTRLYQIEKYRAPKESYEA SLDTWTRDLEFHAVQDKKPKEQEKRGDFLHRPFSKKLDKNLPSHKQPSGSLMTQIQNTKALLKDRKASKS SFPDKDSFGCRNIFRKTSDSNCVASSSMEFIPVPPPRTPRIVKKPEPHQPGPGSTGIPHKEDPLMLDMVR SFESVDRDDHVEVLSPSHHYRILNSPVSLARRNSSERTLSPGLPSGSMSPLHTPLHPTLVSFAHEDKNSP PKEEGLCCPPPVPSNGPMQPLGSPNCVKSRGRFPMMGIGQMLRKRHQSLQPSADRPLEASLPPLQPLAPV NLAFDMADGVKTQC TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_014586 |
| ORF Size | 2142 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_014586.1, NP_055401.1 |
| RefSeq Size | 7385 bp |
| RefSeq ORF | 2145 bp |
| Locus ID | 30811 |
| UniProt ID | P57058 |
| Protein Families | Druggable Genome, Protein Kinase |
| MW | 79.5 kDa |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC216803L1 | Lenti ORF clone of Human hormonally up-regulated Neu-associated kinase (HUNK), Myc-DDK-tagged |
CNY 7,632.00 |
|
| RC216803L2 | Lenti ORF clone of Human hormonally up-regulated Neu-associated kinase (HUNK), mGFP tagged |
CNY 7,700.00 |
|
| RC216803L3 | Lenti ORF clone of Human hormonally up-regulated Neu-associated kinase (HUNK), Myc-DDK-tagged |
CNY 7,700.00 |
|
| RC216803L4 | Lenti ORF clone of Human hormonally up-regulated Neu-associated kinase (HUNK), mGFP tagged |
CNY 7,700.00 |
|
| RG216803 | HUNK (tGFP-tagged) - Human hormonally up-regulated Neu-associated kinase (HUNK) |
CNY 6,832.00 |
|
| SC304134 | HUNK (untagged)-Human hormonally up-regulated Neu-associated kinase (HUNK) |
CNY 5,240.00 |
