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VR1 (TRPV1) (NM_080704) Human Tagged ORF Clone
CAT＃: RC217653
- TrueORF®
TRPV1 (Myc-DDK-tagged)-Human transient receptor potential cation channel, subfamily V, member 1 (TRPV1), transcript variant 1
ORF Plasmid: tGFP
"NM_080704" in other vectors (6)
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CNY 3,990.00
| Cited in 5 publications. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | VR1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC217653 representing NM_080704
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGAAATGGAGCAGCACAGACTTGGGGGCAGCTGCGGACCCACTCCAAAAGGACACCTGCCCAGACC CCCTGGATGGAGACCCTAACTCCAGGCCACCTCCAGCCAAGCCCCAGCTCTCCACGGCCAAGAGCCGCAC CCGGCTCTTTGGGAAGGGTGACTCGGAGGAGGCTTTCCCAGTGGATTGCCCTCACGAGGAAGGTGAGCTG GACTCCTGCCCGACCATCACAGTCAGCCCTGTTATCACCATCCAGAGGCCAGGAGACGGCCCCACCGGTG CCAGGCTGCTGTCCCAGGACTCTGTCGCCGCCAGCACCGAGAAGACCCTCAGGCTCTATGATCGCAGGAG TATCTTTGAAGCCGTTGCTCAGAATAACTGCCAGGATCTGGAGAGCCTGCTGCTCTTCCTGCAGAAGAGC AAGAAGCACCTCACAGACAACGAGTTCAAAGACCCTGAGACAGGGAAGACCTGTCTGCTGAAAGCCATGC TCAACCTGCACGACGGACAGAACACCACCATCCCCCTGCTCCTGGAGATCGCGCGGCAAACGGACAGCCT GAAGGAGCTTGTCAACGCCAGCTACACGGACAGCTACTACAAGGGCCAGACAGCACTGCACATCGCCATC GAGAGACGCAACATGGCCCTGGTGACCCTCCTGGTGGAGAACGGAGCAGACGTCCAGGCTGCGGCCCATG GGGACTTCTTTAAGAAAACCAAAGGGCGGCCTGGATTCTACTTCGGTGAACTGCCCCTGTCCCTGGCCGC GTGCACCAACCAGCTGGGCATCGTGAAGTTCCTGCTGCAGAACTCCTGGCAGACGGCCGACATCAGCGCC AGGGACTCGGTGGGCAACACGGTGCTGCACGCCCTGGTGGAGGTGGCCGACAACACGGCCGACAACACGA AGTTTGTGACGAGCATGTACAATGAGATTCTGATGCTGGGGGCCAAACTGCACCCGACGCTGAAGCTGGA GGAGCTCACCAACAAGAAGGGAATGACGCCGCTGGCTCTGGCAGCTGGGACCGGGAAGATCGGGGTCTTG GCCTATATTCTCCAGCGGGAGATCCAGGAGCCCGAGTGCAGGCACCTGTCCAGGAAGTTCACCGAGTGGG CCTACGGGCCCGTGCACTCCTCGCTGTACGACCTGTCCTGCATCGACACCTGCGAGAAGAACTCGGTGCT GGAGGTGATCGCCTACAGCAGCAGCGAGACCCCTAATCGCCACGACATGCTCTTGGTGGAGCCGCTGAAC CGACTCCTGCAGGACAAGTGGGACAGATTCGTCAAGCGCATCTTCTACTTCAACTTCCTGGTCTACTGCC TGTACATGATCATCTTCACCATGGCTGCCTACTACAGGCCCGTGGATGGCTTGCCTCCCTTTAAGATGGA AAAAATTGGAGACTATTTCCGAGTTACTGGAGAGATCCTGTCTGTGTTAGGAGGAGTCTACTTCTTTTTC CGAGGGATTCAGTATTTCCTGCAGAGGCGGCCGTCGATGAAGACCCTGTTTGTGGACAGCTACAGTGAGA TGCTTTTCTTTCTGCAGTCACTGTTCATGCTGGCCACCGTGGTGCTGTACTTCAGCCACCTCAAGGAGTA TGTGGCTTCCATGGTATTCTCCCTGGCCTTGGGCTGGACCAACATGCTCTACTACACCCGCGGTTTCCAG CAGATGGGCATCTATGCCGTCATGATAGAGAAGATGATCCTGAGAGACCTGTGCCGTTTCATGTTTGTCT ACGTCGTCTTCTTGTTCGGGTTTTCCACAGCGGTGGTGACGCTGATTGAAGACGGGAAGAATGACTCCCT GCCGTCTGAGTCCACGTCGCACAGGTGGCGGGGGCCTGCCTGCAGGCCCCCCGATAGCTCCTACAACAGC CTGTACTCCACCTGCCTGGAGCTGTTCAAGTTCACCATCGGCATGGGCGACCTGGAGTTCACTGAGAACT ATGACTTCAAGGCTGTCTTCATCATCCTGCTGCTGGCCTATGTAATTCTCACCTACATCCTCCTGCTCAA CATGCTCATCGCCCTCATGGGTGAGACTGTCAACAAGATCGCACAGGAGAGCAAGAACATCTGGAAGCTG CAGAGAGCCATCACCATCCTGGACACGGAGAAGAGCTTCCTTAAGTGCATGAGGAAGGCCTTCCGCTCAG GCAAGCTGCTGCAGGTGGGGTACACACCTGATGGCAAGGACGACTACCGGTGGTGCTTCAGGGTGGACGA GGTGAACTGGACCACCTGGAACACCAACGTGGGCATCATCAACGAAGACCCGGGCAACTGTGAGGGCGTC AAGCGCACCCTGAGCTTCTCCCTGCGGTCAAGCAGAGTTTCAGGCAGACACTGGAAGAACTTTGCCCTGG TCCCCCTTTTAAGAGAGGCAAGTGCTCGAGATAGGCAGTCTGCTCAGCCCGAGGAAGTTTATCTGCGACA GTTTTCAGGGTCTCTGAAGCCAGAGGACGCTGAGGTCTTCAAGAGTCCTGCCGCTTCCGGGGAGAAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC217653 representing NM_080704
Red=Cloning site Green=Tags(s) MKKWSSTDLGAAADPLQKDTCPDPLDGDPNSRPPPAKPQLSTAKSRTRLFGKGDSEEAFPVDCPHEEGEL DSCPTITVSPVITIQRPGDGPTGARLLSQDSVAASTEKTLRLYDRRSIFEAVAQNNCQDLESLLLFLQKS KKHLTDNEFKDPETGKTCLLKAMLNLHDGQNTTIPLLLEIARQTDSLKELVNASYTDSYYKGQTALHIAI ERRNMALVTLLVENGADVQAAAHGDFFKKTKGRPGFYFGELPLSLAACTNQLGIVKFLLQNSWQTADISA RDSVGNTVLHALVEVADNTADNTKFVTSMYNEILMLGAKLHPTLKLEELTNKKGMTPLALAAGTGKIGVL AYILQREIQEPECRHLSRKFTEWAYGPVHSSLYDLSCIDTCEKNSVLEVIAYSSSETPNRHDMLLVEPLN RLLQDKWDRFVKRIFYFNFLVYCLYMIIFTMAAYYRPVDGLPPFKMEKIGDYFRVTGEILSVLGGVYFFF RGIQYFLQRRPSMKTLFVDSYSEMLFFLQSLFMLATVVLYFSHLKEYVASMVFSLALGWTNMLYYTRGFQ QMGIYAVMIEKMILRDLCRFMFVYVVFLFGFSTAVVTLIEDGKNDSLPSESTSHRWRGPACRPPDSSYNS LYSTCLELFKFTIGMGDLEFTENYDFKAVFIILLLAYVILTYILLLNMLIALMGETVNKIAQESKNIWKL QRAITILDTEKSFLKCMRKAFRSGKLLQVGYTPDGKDDYRWCFRVDEVNWTTWNTNVGIINEDPGNCEGV KRTLSFSLRSSRVSGRHWKNFALVPLLREASARDRQSAQPEEVYLRQFSGSLKPEDAEVFKSPAASGEK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_080704 |
| ORF Size | 2517 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_080704.4 |
| RefSeq Size | 4191 bp |
| RefSeq ORF | 2520 bp |
| Locus ID | 7442 |
| UniProt ID | Q8NER1 |
| Protein Families | Druggable Genome, Ion Channels: Transient receptor potential, Transmembrane |
| Protein Pathways | Neuroactive ligand-receptor interaction |
| MW | 95 kDa |
| Gene Summary | Capsaicin, the main pungent ingredient in hot chili peppers, elicits a sensation of burning pain by selectively activating sensory neurons that convey information about noxious stimuli to the central nervous system. The protein encoded by this gene is a receptor for capsaicin and is a non-selective cation channel that is structurally related to members of the TRP family of ion channels. This receptor is also activated by increases in temperature in the noxious range, suggesting that it functions as a transducer of painful thermal stimuli in vivo. Four transcript variants encoding the same protein, but with different 5' UTR sequence, have been described for this gene. [provided by RefSeq, Jul 2008] |
Citations (5)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
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The ubiquitin E3 ligase Nedd4-2 relieves mechanical allodynia through the ubiquitination of TRPA1 channel in db/db mice
,Wang, S;Qi, S;Kogure, Y;Kanda, H;Tian, L;Yamamoto, S;Noguchi, K;Dai, Y;,
The European journal of neuroscience
,PubMed ID 33236491
[VR1]
|
|
Liquiritin, a Novel Inhibitor of TRPV1 and TRPA1, Protects Against LPS-induced Acute Lung Injury
,Liu, Z;Wang, P;Shanshan, L;Guo, R;Gao, W;Tong, H;Yin, Y;Han, X;Liu, T;Chen, X;Zhu, M;Yang, Z;,
Cell Calcium
,PubMed ID 32388008
[VR1]
|
|
4,5-Di-O-Caffeoylquinic Acid from Ligularia fischeri Suppresses Inflammatory Responses Through TRPV1 Activation
,Kim, Y;Kim, JT;Park, J;Son, HJ;Kim, EY;Lee, YJ;Rhyu, MR;,
Phytother Res
,PubMed ID 28782267
[VR1]
|
|
Analgesic effect of dimethyl trisulfide in mice is mediated by TRPA1 and sst4 receptors
,Pozsgai, G;Payrits, M;Sághy, É;Sebestyén-Bátai, R;Steen, E;Szőke, É;Sándor, Z;Solymár, M;Garami, A;Orvos, P;Tálosi, L;Helyes, Z;Pintér, E;,
Nitric Oxide
,PubMed ID 28137611
[VR1]
|
|
Agonist-dependence of functional properties for common nonsynonymous variants of human transient receptor potential vanilloid 1
,Wang, S;Joseph, J;Diatchenko, L;Ro, JY;Chung, MK;,
Pain
,PubMed ID 26967694
[VR1]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC217653L1 | Lenti ORF clone of Human transient receptor potential cation channel, subfamily V, member 1 (TRPV1), transcript variant 1, Myc-DDK-tagged |
CNY 8,544.00 |
|
| RC217653L2 | Lenti ORF clone of Human transient receptor potential cation channel, subfamily V, member 1 (TRPV1), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RC217653L3 | Lenti ORF clone of Human transient receptor potential cation channel, subfamily V, member 1 (TRPV1), transcript variant 1, Myc-DDK-tagged |
CNY 8,544.00 |
|
| RC217653L4 | Lenti ORF clone of Human transient receptor potential cation channel, subfamily V, member 1 (TRPV1), transcript variant 1, mGFP tagged |
CNY 8,544.00 |
|
| RG217653 | TRPV1 (tGFP-tagged) - Human transient receptor potential cation channel, subfamily V, member 1 (TRPV1), transcript variant 1 |
CNY 7,744.00 |
|
| SC128105 | TRPV1 (untagged)-Human transient receptor potential cation channel, subfamily V, member 1 (TRPV1), transcript variant 1 |
CNY 6,152.00 |
