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TRF1 (TERF1) (NM_017489) Human Tagged ORF Clone
CAT＃: RC217957
TERF1 (Myc-DDK-tagged)-Human telomeric repeat binding factor (NIMA-interacting) 1 (TERF1), transcript variant 1
ORF Plasmid: tGFP
"NM_017489" in other vectors (6)
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CNY 5,488.00
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | hTRF1-AS; PIN2; t-TRF1; TRBF1; TRF; TRF1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC217957 representing NM_017489
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGAGGATGTTTCCTCAGCGGCCCCGAGCCCGCGGGGCTGTGCGGATGGTAGGGATGCCGACCCTA CTGAGGAGCAGATGGCAGAAACAGAGAGAAACGACGAGGAGCAGTTCGAATGCCAGGAACTGCTCGAGTG CCAGGTGCAGGTGGGGGCCCCCGAGGAGGAGGAGGAGGAGGAGGAGGACGCGGGCCTGGTGGCCGAGGCC GAGGCCGTGGCTGCCGGCTGGATGCTCGATTTCCTCTGCCTCTCTCTTTGCCGAGCTTTCCGCGACGGCC GCTCCGAGGACTTCCGCAGGACCCGCAACAGCGCAGAGGCTATTATTCATGGACTATCCAGTCTAACAGC TTGCCAGTTGAGAACGATATACATATGTCAGTTTTTGACAAGAATTGCAGCAGGAAAAACCCTTGATGCA CAGTTTGAAAATGATGAACGAATTACACCCTTGGAATCAGCCCTGATGATTTGGGGTTCAATTGAAAAGG AACATGACAAACTTCATGAAGAAATACAGAATTTAATTAAAATTCAGGCTATAGCTGTTTGTATGGAAAA TGGCAACTTTAAAGAAGCAGAAGAAGTCTTTGAAAGAATATTTGGTGATCCAAATTCTCATATGCCTTTC AAAAGCAAATTGCTTATGATAATCTCTCAGAAAGATACATTTCATTCCTTTTTTCAACACTTCAGCTACA ACCACATGATGGAGAAAATTAAGAGTTATGTGAATTATGTGCTAAGTGAAAAATCATCAACCTTTCTAAT GAAGGCAGCGGCAAAAGTAGTAGAAAGCAAAAGGACAAGAACAATAACTTCTCAAGATAAACCTAGTGGT AATGATGTTGAAATGGAAACTGAAGCTAATTTGGATACAAGAAAAAGTGTTAGTGACAAACAGTCTGCGG TAACTGAATCCTCAGAGGGTACAGTATCCTTATTGAGGTCTCACAAGAATCTTTTCTTATCTAAGTTGCA ACATGGAACCCAGCAACAAGACCTTAATAAGAAAGAAAGAAGAGTAGGAACTCCTCAAAGTACAAAAAAG AAAAAAGAAAGCAGAAGAGCCACTGAAAGCAGAATACCTGTTTCAAAGAGTCAGCCGGTAACTCCTGAAA AACATCGAGCTAGAAAAAGACAGGCATGGCTTTGGGAAGAAGACAAGAATTTGAGATCTGGCGTGAGGAA ATATGGAGAGGGAAACTGGTCTAAAATACTGTTGCATTATAAATTCAACAACCGGACAAGTGTCATGTTA AAAGACAGATGGAGGACCATGAAGAAACTAAAACTGATTTCCTCAGACAGCGAAGAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC217957 representing NM_017489
Red=Cloning site Green=Tags(s) MAEDVSSAAPSPRGCADGRDADPTEEQMAETERNDEEQFECQELLECQVQVGAPEEEEEEEEDAGLVAEA EAVAAGWMLDFLCLSLCRAFRDGRSEDFRRTRNSAEAIIHGLSSLTACQLRTIYICQFLTRIAAGKTLDA QFENDERITPLESALMIWGSIEKEHDKLHEEIQNLIKIQAIAVCMENGNFKEAEEVFERIFGDPNSHMPF KSKLLMIISQKDTFHSFFQHFSYNHMMEKIKSYVNYVLSEKSSTFLMKAAAKVVESKRTRTITSQDKPSG NDVEMETEANLDTRKSVSDKQSAVTESSEGTVSLLRSHKNLFLSKLQHGTQQQDLNKKERRVGTPQSTKK KKESRRATESRIPVSKSQPVTPEKHRARKRQAWLWEEDKNLRSGVRKYGEGNWSKILLHYKFNNRTSVML KDRWRTMKKLKLISSDSED TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_017489 |
| ORF Size | 1317 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_017489.3 |
| RefSeq Size | 2686 bp |
| RefSeq ORF | 1320 bp |
| Locus ID | 7013 |
| UniProt ID | P54274 |
| Domains | myb_DNA-binding |
| Protein Families | Transcription Factors |
| MW | 50.1 kDa |
| Gene Summary | This gene encodes a telomere specific protein which is a component of the telomere nucleoprotein complex. This protein is present at telomeres throughout the cell cycle and functions as an inhibitor of telomerase, acting in cis to limit the elongation of individual chromosome ends. The protein structure contains a C-terminal Myb motif, a dimerization domain near its N-terminus and an acidic N-terminus. Two transcripts of this gene are alternatively spliced products. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC217957L1 | Lenti ORF clone of Human telomeric repeat binding factor (NIMA-interacting) 1 (TERF1), transcript variant 1, Myc-DDK-tagged |
CNY 7,888.00 |
|
| RC217957L2 | Lenti ORF clone of Human telomeric repeat binding factor (NIMA-interacting) 1 (TERF1), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RC217957L3 | Lenti ORF clone of Human telomeric repeat binding factor (NIMA-interacting) 1 (TERF1), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC217957L4 | Lenti ORF clone of Human telomeric repeat binding factor (NIMA-interacting) 1 (TERF1), transcript variant 1, mGFP tagged |
CNY 7,888.00 |
|
| RG217957 | TERF1 (tGFP-tagged) - Human telomeric repeat binding factor (NIMA-interacting) 1 (TERF1), transcript variant 1 |
CNY 7,088.00 |
|
| SC308887 | TERF1 (untagged)-Human telomeric repeat binding factor (NIMA-interacting) 1 (TERF1), transcript variant 1 |
CNY 5,488.00 |
