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MURF3 (TRIM54) (NM_032546) Human Tagged ORF Clone
CAT＃: RC218075
TRIM54 (Myc-DDK-tagged)-Human tripartite motif containing 54 (TRIM54), transcript variant 1
ORF Plasmid: tGFP
"NM_032546" in other vectors (6)
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CNY 3,656.00
CNY 3,990.00
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | MURF; MURF-3; muRF3; RNF30 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC218075 representing NM_032546
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAACTTCACAGTGGGTTTCAAGCCGCTGCTAGGGGATGCACACAGCATGGACAACCTGGAGAAGCAGC TCATCTGCCCCATCTGCCTGGAGATGTTCTCCAAACCAGTGGTGATCCTGCCCTGCCAACACAACCTGTG CCGCAAATGTGCCAACGACGTCTTCCAGGCCTCGAATCCTCTATGGCAGTCCCGGGGCTCCACCACTGTG TCTTCAGGAGGCCGTTTCCGCTGCCCATCGTGCAGGCATGAGGTTGTCCTGGACAGACACGGTGTCTACG GCCTGCAGCGAAACCTGCTAGTGGAGAACATTATCGACATTTACAAGCAGGAGTCATCCAGGCCGCTGCA CTCCAAGGCTGAGCAGCACCTCATGTGCGAGGAGCATGAAGAAGAGAAGATCAATATTTACTGCCTGAGC TGTGAGGTGCCCACCTGCTCTCTCTGCAAGGTCTTCGGTGCCCACAAGGACTGTGAGGTGGCCCCACTGC CCACCATTTACAAACGCCAGAAGAAACAGGATCTCACTCTGTTGCCCAGGCTGGAGTGCAGTGGCACAAA CACAACTTACTGCAGCCTTGATCTCCCGAGCTCAAGTGATCCTCCCATCTTAGCCTCGCAGAACACTAAG ATTATAGATAGTGAGCTCAGCGATGGCATCGCGATGCTGGTGGCAGGCAATGACCGCGTGCAAGCAGTGA TCACACAGATGGAGGAGGTGTGCCAGACTATCGAGGACAATAGCCGGAGGCAGAAGCAGTTGTTAAACCA GAGGTTTGAGAGCCTGTGCGCAGTGCTGGAGGAGCGCAAGGGTGAGCTGCTGCAGGCGCTGGCCCGGGAG CAAGAGGAGAAGCTGCAGCGCGTCCGCGGCCTCATCCGTCAGTATGGCGACCACCTGGAGGCCTCCTCTA AGCTGGTGGAGTCTGCCATCCAGTCCATGGAAGAGCCACAAATGGCGCTGTATCTCCAGCAGGCCAAGGA GCTGATCAATAAGGTCGGGGCCATGTCGAAGGTGGAGCTGGCAGGGCGGCCGGAGCCAGGCTATGAGAGC ATGGAGCAATTCACCGTAAGGGTGGAGCACGTGGCCGAAATGCTGCGGACCATCGACTTCCAGCCAGGCG CTTCCGGGGAGGAAGAGGAGGTGGCCCCAGACGGAGAGGAGGGCAGCGCGGGGCCGGAGGAAGAGCGGCC GGATGGGCCT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC218075 representing NM_032546
Red=Cloning site Green=Tags(s) MNFTVGFKPLLGDAHSMDNLEKQLICPICLEMFSKPVVILPCQHNLCRKCANDVFQASNPLWQSRGSTTV SSGGRFRCPSCRHEVVLDRHGVYGLQRNLLVENIIDIYKQESSRPLHSKAEQHLMCEEHEEEKINIYCLS CEVPTCSLCKVFGAHKDCEVAPLPTIYKRQKKQDLTLLPRLECSGTNTTYCSLDLPSSSDPPILASQNTK IIDSELSDGIAMLVAGNDRVQAVITQMEEVCQTIEDNSRRQKQLLNQRFESLCAVLEERKGELLQALARE QEEKLQRVRGLIRQYGDHLEASSKLVESAIQSMEEPQMALYLQQAKELINKVGAMSKVELAGRPEPGYES MEQFTVRVEHVAEMLRTIDFQPGASGEEEEVAPDGEEGSAGPEEERPDGP TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_032546 |
| ORF Size | 1200 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_032546.4 |
| RefSeq Size | 1770 bp |
| RefSeq ORF | 1203 bp |
| Locus ID | 57159 |
| UniProt ID | Q9BYV2 |
| MW | 44.7 kDa |
| Gene Summary | The protein encoded by this gene contains a RING finger motif and is highly similar to the ring finger proteins RNF28/MURF1 and RNF29/MURF2. In vitro studies demonstrated that this protein, RNF28, and RNF29 form heterodimers, which may be important for the regulation of titin kinase and microtubule-dependent signal pathways in striated muscles. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC218075L1 | Lenti ORF clone of Human tripartite motif containing 54 (TRIM54), transcript variant 1, Myc-DDK-tagged |
CNY 6,056.00 |
|
| RC218075L2 | Lenti ORF clone of Human tripartite motif containing 54 (TRIM54), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RC218075L3 | Lenti ORF clone of Human tripartite motif containing 54 (TRIM54), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC218075L4 | Lenti ORF clone of Human tripartite motif containing 54 (TRIM54), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RG218075 | TRIM54 (tGFP-tagged) - Human tripartite motif containing 54 (TRIM54), transcript variant 1 |
CNY 5,256.00 |
|
| SC305470 | TRIM54 (untagged)-Human tripartite motif containing 54 (TRIM54), transcript variant 1 |
CNY 3,656.00 |
