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METTL9 (NM_001077180) Human Tagged ORF Clone
CAT＃: RC218283
METTL9 (Myc-DDK-tagged)-Human methyltransferase like 9 (METTL9), transcript variant 2
ORF Plasmid: tGFP
"NM_001077180" in other vectors (4)
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CNY 2,400.00
CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | CGI-81; DREV; DREV1; PAP1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC218283 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGACTGCTGGCGGGCTGGCTGTGCCTGAGCCTGGCGTCCGTGTGGCTGGCGCGGAGGATGTGGACGC TGCGGAGCCCGCTCACCCGCTCCCTGTACGTGAACATGACTAGCGGCCCGGGTGGGCCGGCGGCGGCCGC GGGCGGCAGGAAGGAGAACCACCAGTGGTATGTGTGCAACAGAGAGAAATTATGCGAATCACTCCAGGCT GTCTTTGTTCAGAGTTACCTTGATCAAGGAACACAGATCTTCTTAAACAACAGCATTGAGAAATCGGGCT GGCTATTTATCCAATTATATCATTCTTTTGTGTCATCTGTTTTTAGCCTGTTTATGTCTAGAACATCTAT CAATGGGTTGCTAGGAAGAGGCTCAATGTTTGTGTTTTCACCAGATCAGTTTCAGAGACTGCTTAAAATT AATCCAGACTGGAAAACCCACAGACTTCTTGATTTAGGTGCTGGAGATGGAGAAGTCACAAAAATCATGA GCCCTCATTTTGAAGAAATCTATGCCACTGAGCTTTCTGAAACTATGATATGGCAGCTTCAGAAAAAGAA ATACAGAGTCCTTGGTATAAATGAATGGCAGAATACGGGGTTCCAGTATGATGTCATCAGCTGCCTGAAC TTGCTGGACCGCTGTGATCAGCCCCTGACTTTGTTAAAAGATATCAGAAGTGTCTTGGAGCCAACTAGAG GCAGGGTCATCCTTGCCCTTGTCCTCCCCTTTCATCCCTATGTGGAAAACGTAGGTGGCAAGTGGGAGAA ACCATCAGAAATTTTGGAAATCAAAGGACAGAACTGGGAAGAACAAGTGAATAGTCTGCCTGAAGTTTTC AGAAAAGCTGGTTTTGTTATCGAAGCTTTCACCAGACTACCATACCTGTGTGAAGGCGACATGTATAATG ACTACTACGTTCTGGATGACGCTGTCTTTGTTCTCAAACCAGTA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC218283 protein sequence
Red=Cloning site Green=Tags(s) MRLLAGWLCLSLASVWLARRMWTLRSPLTRSLYVNMTSGPGGPAAAAGGRKENHQWYVCNREKLCESLQA VFVQSYLDQGTQIFLNNSIEKSGWLFIQLYHSFVSSVFSLFMSRTSINGLLGRGSMFVFSPDQFQRLLKI NPDWKTHRLLDLGAGDGEVTKIMSPHFEEIYATELSETMIWQLQKKKYRVLGINEWQNTGFQYDVISCLN LLDRCDQPLTLLKDIRSVLEPTRGRVILALVLPFHPYVENVGGKWEKPSEILEIKGQNWEEQVNSLPEVF RKAGFVIEAFTRLPYLCEGDMYNDYYVLDDAVFVLKPV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001077180 |
| ORF Size | 954 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq Size | 3264 bp |
| RefSeq ORF | 954 bp |
| Locus ID | 51108 |
| UniProt ID | Q9H1A3 |
| MW | 36.5 kDa |
| Gene Summary | Protein-histidine N-methyltransferase that specifically catalyzes 1-methylhistidine (pros-methylhistidine) methylation of target proteins (PubMed:33563959). Mediates methylation of proteins with a His-x-His (HxH) motif (where 'x' is preferably a small amino acid) (PubMed:33563959). Catalyzes methylation of target proteins such as S100A9, NDUFB3, SLC39A5, SLC39A7, ARMC6 and DNAJB12; 1-methylhistidine modification may affect the binding of zinc and other metals to its target proteins (PubMed:33563959). Constitutes the main methyltransferase for the 1-methylhistidine modification in cell (PubMed:33563959).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC218283L3 | Lenti ORF clone of Human methyltransferase like 9 (METTL9), transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC218283L4 | Lenti ORF clone of Human methyltransferase like 9 (METTL9), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
| RG218283 | METTL9 (tGFP-tagged) - Human methyltransferase like 9 (METTL9), transcript variant 2 |
CNY 4,370.00 |
|
| SC315418 | METTL9 (untagged)-Human methyltransferase like 9 (METTL9), transcript variant 2 |
CNY 6,270.00 |
