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PRPF31 (NM_015629) Human Tagged ORF Clone
CAT＃: RC218307
PRPF31 (Myc-DDK-tagged)-Human PRP31 pre-mRNA processing factor 31 homolog (S. cerevisiae) (PRPF31)
ORF Plasmid: tGFP
"NM_015629" in other vectors (4)
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CNY 3,656.00
CNY 3,990.00
| Cited in 4 publications. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | NY-BR-99; PRP31; RP11; SNRNP61 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC218307 representing NM_015629
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTCTGGCAGATGAGCTCTTAGCTGATCTCGAAGAGGCAGCAGAAGAGGAGGAAGGAGGAAGCTATG GGGAGGAAGAAGAGGAGCCAGCGATCGAGGATGTGCAGGAGGAGACACAGCTGGATCTTTCCGGGGATTC AGTCAAGACCATCGCCAAGCTATGGGATAGTAAGATGTTTGCTGAGATTATGATGAAGATTGAGGAGTAT ATCAGCAAGCAAGCCAAAGCTTCAGAAGTGATGGGACCAGTGGAGGCCGCGCCTGAATACCGCGTCATCG TGGATGCCAACAACCTGACCGTGGAGATCGAAAACGAGCTGAACATCATCCATAAGTTCATCCGGGATAA GTACTCAAAGAGATTCCCTGAACTGGAGTCCTTGGTCCCCAATGCACTGGATTACATCCGCACGGTCAAG GAGCTGGGCAACAGCCTGGACAAGTGCAAGAACAATGAGAACCTGCAGCAGATCCTCACCAATGCCACCA TCATGGTCGTCAGCGTCACCGCCTCCACCACCCAGGGGCAGCAGCTGTCGGAGGAGGAGCTGGAGCGGCT GGAGGAGGCCTGCGACATGGCGCTGGAGCTGAACGCCTCCAAGCACCGCATCTACGAGTATGTGGAGTCC CGGATGTCCTTCATCGCACCCAACCTGTCCATCATTATCGGGGCATCCACGGCCGCCAAGATCATGGGTG TGGCCGGCGGCCTGACCAACCTCTCCAAGATGCCTGCCTGCAACATCATGCTGCTCGGGGCCCAGCGCAA GACGCTGTCGGGCTTCTCGTCTACCTCAGTGCTGCCCCACACCGGCTACATCTACCACAGTGACATCGTG CAGTCCCTGCCACCGGATCTGCGGCGGAAAGCGGCCCGGCTGGTGGCCGCCAAGTGCACACTGGCAGCCC GTGTGGACAGTTTCCACGAGAGCACAGAAGGGAAGGTGGGCTACGAACTGAAGGATGAGATCGAGCGCAA ATTCGACAAGTGGCAGGAGCCGCCGCCTGTGAAGCAGGTGAAGCCGCTGCCTGCGCCCCTGGATGGACAG CGGAAGAAGCGAGGCGGCCGCAGGTACCGCAAGATGAAGGAGCGGCTGGGGCTGACGGAGATCCGGAAGC AGGCCAACCGTATGAGCTTCGGAGAGATCGAGGAGGACGCCTACCAGGAGGACCTGGGATTCAGCCTGGG CCACCTGGGCAAGTCGGGCAGTGGGCGTGTGCGGCAGACACAGGTAAACGAGGCCACCAAGGCCAGGATC TCCAAGACGCTGCAGCGGACCCTGCAGAAGCAGAGCGTCGTATATGGCGGGAAGTCCACCATCCGCGACC GCTCCTCGGGCACGGCCTCCAGCGTGGCCTTCACCCCACTCCAGGGCCTGGAGATTGTGAACCCACAGGC GGCAGAGAAGAAGGTGGCTGAGGCCAACCAGAAGTATTTCTCCAGCATGGCTGAGTTCCTCAAGGTTAAG GGCGAGAAGAGTGGCCTTATGTCCACC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC218307 representing NM_015629
Red=Cloning site Green=Tags(s) MSLADELLADLEEAAEEEEGGSYGEEEEEPAIEDVQEETQLDLSGDSVKTIAKLWDSKMFAEIMMKIEEY ISKQAKASEVMGPVEAAPEYRVIVDANNLTVEIENELNIIHKFIRDKYSKRFPELESLVPNALDYIRTVK ELGNSLDKCKNNENLQQILTNATIMVVSVTASTTQGQQLSEEELERLEEACDMALELNASKHRIYEYVES RMSFIAPNLSIIIGASTAAKIMGVAGGLTNLSKMPACNIMLLGAQRKTLSGFSSTSVLPHTGYIYHSDIV QSLPPDLRRKAARLVAAKCTLAARVDSFHESTEGKVGYELKDEIERKFDKWQEPPPVKQVKPLPAPLDGQ RKKRGGRRYRKMKERLGLTEIRKQANRMSFGEIEEDAYQEDLGFSLGHLGKSGSGRVRQTQVNEATKARI SKTLQRTLQKQSVVYGGKSTIRDRSSGTASSVAFTPLQGLEIVNPQAAEKKVAEANQKYFSSMAEFLKVK GEKSGLMST TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_015629 |
| ORF Size | 1497 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_015629.4 |
| RefSeq Size | 2153 bp |
| RefSeq ORF | 1500 bp |
| Locus ID | 26121 |
| UniProt ID | Q8WWY3 |
| Domains | Nop |
| Protein Families | Druggable Genome |
| Protein Pathways | Spliceosome |
| MW | 55.3 kDa |
| Gene Summary | This gene encodes a component of the spliceosome complex and is one of several retinitis pigmentosa-causing genes. When the gene product is added to the spliceosome complex, activation occurs.[provided by RefSeq, Jan 2009] |
Citations (4)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
A CRISPR and high-content imaging assay compliant with ACMG/AMP guidelines for clinical variant interpretation in ciliopathies
,Nazlamova, L;Thomas, NS;Cheung, MK;Legebeke, J;Lord, J;Pengelly, RJ;Tapper, WJ;Wheway, G;,
Hum Genet
,PubMed ID 33095315
[PRPF31]
|
|
High-throughput PRPF31 variant characterisation pipeline consistent with ACMG/AMP clinical variant interpretation guidelines
,Nazlamova, LA;Cheung, MK;Legebeke, J;Lord, J;,
medRxiv
[PRPF31]
|
|
A Combined in silico, in vitro and Clinical Approach to Characterize Novel Pathogenic Missense Variants in PRPF31 in Retinitis Pigmentosa
,Wheway, G;Nazlamova, L;Meshad, N;Hunt, S;Jackson, N;Churchill, A;,
Front Genet
,PubMed ID 30967900
[PRPF31]
|
|
A combined in silico, in vitro and clinical approach to characterise novel pathogenic missense variants in PRPF31 in retinitis pigmentosa
,Wheway, G;Nazlamova, L;Meshad, N;Hunt, S;Jackson, N;Churchill, A;,
bioRxiv
[PRPF31]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC218307L3 | Lenti ORF clone of Human PRP31 pre-mRNA processing factor 31 homolog (S. cerevisiae) (PRPF31), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC218307L4 | Lenti ORF clone of Human PRP31 pre-mRNA processing factor 31 homolog (S. cerevisiae) (PRPF31), mGFP tagged |
CNY 5,890.00 |
|
| RG218307 | PRPF31 (tGFP-tagged) - Human PRP31 pre-mRNA processing factor 31 homolog (S. cerevisiae) (PRPF31) |
CNY 5,256.00 |
|
| SC114632 | PRPF31 (untagged)-Human PRP31 pre-mRNA processing factor 31 homolog (S. cerevisiae) (PRPF31) |
CNY 3,656.00 |
