KALRN (NM_007064) Human Tagged ORF Clone
CAT#: RC218885
KALRN (Myc-DDK-tagged)-Human kalirin, RhoGEF kinase (KALRN), transcript variant 3
ORF Plasmid: tGFP
"NM_007064" in other vectors (6)
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CNY 8,976.00
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | ARHGEF24; CHD5; CHDS5; DUET; DUO; HAPIP; TRAD |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC218885 representing NM_007064
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGGGCGGCGACAGGGCTTACACCCGAGGTCCCTCTTTGGGGTGGCTCTTTGCTAAGTGCTGCTGTT GCTTCCCGTGTAGAGATGCATACTCTCATTCCTCAAGCGAGAATGGAGGCAAGTCCGAGTCCGTGGCCAA CCTGCAGGCCCAGCCCTCCCTGAACTCCATCCACAGTTCCCCGGGTCCCAAGCGCTCCACCAACACTCTT AAGAAGTGGCTGACGAGTCCTGTGCGTCGGCTTAACAGCGGGAAGGCAGATGGAAACATCAAAAAGCAGA AGAAAGTTCGCGATGGTCGGAAGAGCTTTGACCTGGGATCTCCCAAGCCTGGGGATGAAACAACCCCTCA GGGAGACAGCGCTGATGAGAAGAGCAAGAAAGGTTGGGGTGAAGATGAGCCGGATGAAGAGTCACACACA CCCCTCCCACCACCTATGAAGATTTTTGACAACGACCCTACACAGGATGAAATGTCCTCCTCTTTGCTAG CAGCCCGGCAGGCTTCCACTGAAGTACCTACTGCTGCAGACCTTGTCAATGCAATAGAAAAGTTGGTCAA AAACAAGCTGAGTCTAGAAGGAAGCTCATACCGGGGGAGCTTGAAAGACCCTGCAGGCTGCCTGAATGAG GGGATGGCCCCACCCACACCTCCTAAAAACCCAGAAGAAGAACAGAAAGCCAAGGCCCTGAGAGGCAGGA TGTTTGTCCTGAATGAGCTGGTACAGACAGAGAAAGACTATGTCAAGGATCTGGGCATTGTGGTGGAGGG CTTCATGAAGAGAATAGAAGAAAAGGGTGTCCCTGAGGATATGCGAGGAAAGGACAAAATCGTGTTTGGA AATATTCATCAGATTTATGACTGGCATAAGGATTTTTTCCTGGCGGAACTGGAAAAGTGTATCCAGGAGC AAGACAGATTGGCACAGCTCTTTATTAAGCACGAGCGGAAGCTGCACATCTACGTGTGGTATTGTCAGAA TAAGCCGCGCTCAGAGTACATCGTTGCTGAGTATGACGCCTACTTTGAGGAGGTAAAACAGGAGATAAAT CAGAGGCTGACACTGAGTGACTTCCTCATCAAGCCCATTCAGAGAATAACAAAATACCAGTTGCTCCTCA AGGACTTCCTGAGATACAGTGAGAAGGCTGGTTTGGAGTGTTCAGATATTGAGAAAGCAGTGGAGTTAAT GTGCCTTGTTCCCAAACGCTGCAATGACATGATGAATCTAGGACGTCTGCAGGGCTTTGAGGGCACTCTG ACTGCTCAGGGGAAGCTGCTGCAGCAGGACACATTCTATGTGATCGAGCTGGATGCAGGCATGCAGTCCC GGACCAAAGAGAGGCGCGTGTTCCTCTTCGAGCAGATTGTCATCTTCAGTGAACTGCTCAGGAAGGGATC CCTCACCCCTGGCTACATGTTCAAAAGGAGCATCAAGATGAATTACTTGGTCCTGGAGGAGAATGTGGAC AATGATCCCTGCAAGTTTGCACTCATGAACAGAGAGACTTCTGAGAGGGTTGTTCTGCAAGCCGCCAACG CTGACATCCAGCAGGCCTGGGTGCAGGACATCAATCAAGTCTTAGAAACACAGCGAGACTTTTTGAATGC ACTGCAATCGCCCATTGAGTATCAACGGAAAGAAAGGAGCACAGCTGTGATGAGGTCTCAACCTGCCAGG CTTCCCCAAGCCAGCCCCAGGCCCTACTCCTCTGTTCCTGCGGGCTCAGAGAAGCCCCCAAAGGGCTCCA GCTATAACCCACCTCTGCCTCCCCTGAAGATATCTACCTCCAATGGCAGTCCAGGGTTTGAATACCACCA GCCTGGGGACAAGTTCGAAGCCAGCAAGCAGAACGACCTGGGAGGCTGCAATGGGACCTCGTCCATGGCC GTGATCAAAGATTACTATGCACTGAAGGAGAATGAAATCTGTGTGAGCCAAGGTGAGGTGGTCCAGGTCC TCGCCGTCAACCAGCAGAACATGTGTCTGGTGTACCAGCCTGCCAGCGACCATTCCCCCGCCGCCGAGGG CTGGGTCCCAGGCAGCATCCTGGCGCCCCTCACCAAAGCCACAGCAGCAGAAAGTAGTGACGGGAGCATC AAGAAGTCATGTTCATGGCATACTCTACGCATGAGAAAGCGGGCGGAAGTGGAGAACACGGGTAAAAATG AAGCCACAGGGCCTCGTAAACCCAAGGATATTCTGGGCAACAAAGTCTCTGTTAAAGAGACGAACAGTTC CGAGGAATCAGAGTGTGATGATCTTGACCCTAATACTAGCATGGAGATCTTAAATCCAAATTTCATCCAA GAAGTGGCCCCAGAATTCCTTGTGCCCTTGGTGGATGTGACCTGCTTGCTTGGGGACACAGTGATACTGC AGTGCAAAGTCTGTGGGCGGCCAAAGCCCACCATCACTTGGAAGGGTCCAGACCAGAACATCCTTGACAC TGATAACAGCTCAGCCACATACACGGTCTCCTCTTGTGATTCTGGAGAAATCACCCTGAAGATCTGTAAT CTGATGCCCCAAGACAGTGGGATTTATACCTGCATAGCAACAAATGACCACGGGACCACATCAACGTCTG CAACAGTCAAAGTGCAAGGTGTTCCAGCAGCCCCTAACCGCCCCATTGCCCAGGAGAGAAGCTGCACCTC CGTGATTCTCCGCTGGCTGCCCCCCTCCAGCACAGGAAACTGCACTATTTCTGGTTACACTGTGGAGTAC AGAGAGGAAGGTTCTCAGATCTGGCAGCAGTCAGTGGCTTCGACCTTGGACACTTACCTCGTCATCGAAG ACCTTAGTCCCGGGTGTCCTTATCAGTTCAGAGTCAGTGCCAGTAACCCCTGGGGAATCAGCCTTCCCAG CGAGCCCTCGGAGTTTGTGCGACTTCCAGAATATGATGCTGCTGCTGATGGTGCCACCATTTCTTGGAAG GAAAATTTTGACTCAGCTTACACTGAGCTGAATGAAATTGGAAGAGGCCGTTTCTCTATAGTAAAGAAAT GCATTCACAAAGCTACCCGCAAAGATGTGGCTGTGAAATTTGTTAGCAAAAAAATGAAGAAGAAAGAACA GGCTGCCCACGAGGCTGCCCTGCTTCAGCACCTACAGCACCCCCAGTACATCACTCTCCATGACACCTAT GAGTCCCCCACATCCTACATCCTGATCTTGGAACTGATGGATGATGGCCGGCTCTTAGACTACCTTATGA ATCATGATGAACTGATGGAGGAAAAAGTAGCTTTCTATATCCGAGACATCATGGAGGCTCTGCAGTACCT TCACAACTGCAGGGTTGCACATTTGGACATAAAGCCTGAAAACCTGCTCATTGACCTACGGATTCCAGTG CCTCGAGTGAAGCTCATTGACTTGGAGGATGCTGTCCAGATCTCGGGTCACTTCCACATTCACCACCTGC TGGGGAACCCTGAGTTTGCTGCCCCAGAAGTCATTCAAGGCATCCCCGTCTCCCTGGGGACAGACATCTG GAGCATCGGGGTTCTGACATATGTCATGCTGAGTGGGGTCTCCCCCTTCTTGGATGAGAGCAAAGAGGAG ACATGTATCAACGTATGCAGGGTGGATTTCAGCTTCCCCCATGAATACTTCTGTGGTGTGAGCAATGCTG CCAGAGATTTCATCAATGTGATCTTACAGGAAGATTTTCGGAGGCGGCCCACAGCAGCCACATGCTTGCA GCATCCATGGCTGCAGCCCCATAATGGCAGCTACTCTAAGATCCCCCTGGACACCTCCCGCCTAGCATGC TTCATAGAACGTCGCAAGCACCAGAATGATGTGCGGCCTATTCCCAATGTCAAGAGCTACATTGTCAACC GGGTGAACCAAGGGACG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC218885 representing NM_007064
Red=Cloning site Green=Tags(s) MKGGDRAYTRGPSLGWLFAKCCCCFPCRDAYSHSSSENGGKSESVANLQAQPSLNSIHSSPGPKRSTNTL KKWLTSPVRRLNSGKADGNIKKQKKVRDGRKSFDLGSPKPGDETTPQGDSADEKSKKGWGEDEPDEESHT PLPPPMKIFDNDPTQDEMSSSLLAARQASTEVPTAADLVNAIEKLVKNKLSLEGSSYRGSLKDPAGCLNE GMAPPTPPKNPEEEQKAKALRGRMFVLNELVQTEKDYVKDLGIVVEGFMKRIEEKGVPEDMRGKDKIVFG NIHQIYDWHKDFFLAELEKCIQEQDRLAQLFIKHERKLHIYVWYCQNKPRSEYIVAEYDAYFEEVKQEIN QRLTLSDFLIKPIQRITKYQLLLKDFLRYSEKAGLECSDIEKAVELMCLVPKRCNDMMNLGRLQGFEGTL TAQGKLLQQDTFYVIELDAGMQSRTKERRVFLFEQIVIFSELLRKGSLTPGYMFKRSIKMNYLVLEENVD NDPCKFALMNRETSERVVLQAANADIQQAWVQDINQVLETQRDFLNALQSPIEYQRKERSTAVMRSQPAR LPQASPRPYSSVPAGSEKPPKGSSYNPPLPPLKISTSNGSPGFEYHQPGDKFEASKQNDLGGCNGTSSMA VIKDYYALKENEICVSQGEVVQVLAVNQQNMCLVYQPASDHSPAAEGWVPGSILAPLTKATAAESSDGSI KKSCSWHTLRMRKRAEVENTGKNEATGPRKPKDILGNKVSVKETNSSEESECDDLDPNTSMEILNPNFIQ EVAPEFLVPLVDVTCLLGDTVILQCKVCGRPKPTITWKGPDQNILDTDNSSATYTVSSCDSGEITLKICN LMPQDSGIYTCIATNDHGTTSTSATVKVQGVPAAPNRPIAQERSCTSVILRWLPPSSTGNCTISGYTVEY REEGSQIWQQSVASTLDTYLVIEDLSPGCPYQFRVSASNPWGISLPSEPSEFVRLPEYDAAADGATISWK ENFDSAYTELNEIGRGRFSIVKKCIHKATRKDVAVKFVSKKMKKKEQAAHEAALLQHLQHPQYITLHDTY ESPTSYILILELMDDGRLLDYLMNHDELMEEKVAFYIRDIMEALQYLHNCRVAHLDIKPENLLIDLRIPV PRVKLIDLEDAVQISGHFHIHHLLGNPEFAAPEVIQGIPVSLGTDIWSIGVLTYVMLSGVSPFLDESKEE TCINVCRVDFSFPHEYFCGVSNAARDFINVILQEDFRRRPTAATCLQHPWLQPHNGSYSKIPLDTSRLAC FIERRKHQNDVRPIPNVKSYIVNRVNQGT TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_007064 |
ORF Size | 3867 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_007064.5 |
RefSeq Size | 5729 bp |
RefSeq ORF | 3870 bp |
Locus ID | 8997 |
UniProt ID | O60229 |
Domains | RhoGEF, pkinase, TyrKc, PH, S_TKc, ig, IGc2, IG, FN3 |
Protein Families | Druggable Genome, Protein Kinase |
MW | 144.3 kDa |
Gene Summary | Huntington's disease (HD), a neurodegenerative disorder characterized by loss of striatal neurons, is caused by an expansion of a polyglutamine tract in the HD protein huntingtin. This gene encodes a protein that interacts with the huntingtin-associated protein 1, which is a huntingtin binding protein that may function in vesicle trafficking. [provided by RefSeq, Apr 2016] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC218885L1 | Lenti-ORF clone of KALRN (Myc-DDK-tagged)-Human kalirin, RhoGEF kinase (KALRN), transcript variant 3 |
CNY 11,376.00 |
|
RC218885L2 | Lenti-ORF clone of KALRN (mGFP-tagged)-Human kalirin, RhoGEF kinase (KALRN), transcript variant 3 |
CNY 14,730.00 |
|
RC218885L3 | Lenti-ORF clone of KALRN (Myc-DDK-tagged)-Human kalirin, RhoGEF kinase (KALRN), transcript variant 3 |
CNY 14,730.00 |
|
RC218885L4 | Lenti-ORF clone of KALRN (mGFP-tagged)-Human kalirin, RhoGEF kinase (KALRN), transcript variant 3 |
CNY 14,730.00 |
|
RG218885 | KALRN (tGFP-tagged) - Human kalirin, RhoGEF kinase (KALRN), transcript variant 3 |
CNY 14,160.00 |
|
SC309929 | KALRN (untagged)-Human kalirin, RhoGEF kinase (KALRN), transcript variant 3 |
CNY 24,890.00 |